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Random Amplified Polymorphic DNA Technique (0):
Recent article
Random Amplified Polymorphic DNA Technique
Abstract
Rodents are a potential source of pathogenic Yersinia enterocolitica and Y. pseudotuberculosis. In order to study this, 190 rodents were captured and sampled on seven pig farms (n=110), five chicken farms (n=55) and six other locations (n=25) in Sweden. Pigs from three of the pig farms were also samp...
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PMID: 21073763
PDF is available here.
PDF is available here.
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Abstract
To establish sequence characterized amplified region markers of Cornus officinalis and provide a scientific basis for molecular identification of C. officinalis.
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PMID: 21842638
PDF is available here.
PDF is available here.
Abstract
These results altogether indicated that L. borgpetersenii was the dominant species in these areas with serovar Javanica apparently derived from rats which provided an important source of infection in cattle resulting a high incidence of infertility, abortion and.still-birth in the Cauvery river vall...
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PMID: 21706947
PDF is available here.
PDF is available here.
Tropical biomedicine (28)1 2011
Abstract
Our study on genetic diversity among populations of Schistosoma in Saudi Arabia gave a clear picture about this population and subsequently the method for controlling the parasites. From the examination of 40 collected samples of stools and urine, all urine samples were shown to be negative for the...
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PMID: 21602774
PDF is available here.
PDF is available here.
Abstract
We present here data for the spread of Pseudomonas fluorescens caused by a contaminated drinking water dispenser in a bone marrow transplant unit. Over a 1-month period we observed a sharp increase in the isolation of P. fluorescens from weekly pharyngeal surveillance swabs. Environmental samples we...
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PMID: 21450958
PDF is available here.
PDF is available here.
Journal of Vector Ecology (36 Suppl 1) 2011
Abstract
An intraspecific study of Phlebotomus sergenti was performed on populations from Turkey, Syria, Israel, and Uzbekistan by four different approaches: geometric morphometrics, RAPD analysis, internal transcribed spacer 2 (ITS2) sequencing (nuclear marker), and cytochrome B sequencing (mitochondrial mar...
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PMID: 21366780
PDF is available here.
PDF is available here.
Abstract
The highly antagonistic lactobacillus strains isolated from the oral cavity of human individuals were genetically passported as L. fermentum 39, L. rhamnosus 50, and L. rhamnosus 24, by applying the RAPD-PCR technique with two types of primers (M13, MSP). These lactobacillus strains showed high degre...
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PMID: 21506386
PDF is available here.
PDF is available here.
Abstract
A simple protocol for obtaining pure, restrictable and amplifiable megabase genomic DNA from oil-free seed residue of Brassica napus, an important oil seed plant, has been developed. Oil from the dry seeds was completely recovered in an organic solvent and quantified gravimetrically...
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PMID: 21341211
PDF is available here.
PDF is available here.
Vegetative compatibility and genetic analysis of Colletotrichum lindemuthianum isolates from Brazil.
Abstract
We examined variability within and between Brazilian pathotypes of C. lindemuthianum through the identification of vegetative compatibility groups (VCGs) and by RAPD analysis. Two hundred and ninety-five nit mutants were obtained from 47 isolates of various pathotypes of the fungus collected from di...
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PMID: 21341215
PDF is available here.
PDF is available here.
Mikrobiyoloji bulteni (45)1 2011
Abstract
The aims of this study were to analyse the amphotericin B and fluconazole susceptibility and molecular epidemiology of Candida strains (Candida albicans, Candida tropicalis and Candida glabrata) isolated from the urine samples of patients hospitalized in the intensive care unit. Identification of the...
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PMID: 21341168
PDF is available here.
PDF is available here.
Abstract
These results suggest that populations of C. lacinia saundersii are genetically structured....
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PMID: 21152781
PDF is available here.
PDF is available here.
Journal of Insect Physiology (56)12 2010
Abstract
We report that the integrin β1 subunit from hemocytes of Ostrinia furnacalis (Ofint β1) plays an important role in regulating the spreading of plasmatocytes. The full length cDNA sequence (4477 bp) of Ofint β1 was cloned from hemocytes. Phylogenetic analysis showed that Ofint β1 belonged to the...
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PMID: 20708011
PDF is available here.
PDF is available here.
Abstract
We analyzed the genetic variability of Triatoma pseudomaculata in sylvatic and peridomestic ecotopes throughout three localities in the northeastern state of Bahia, Brazil. We studied polymorphisms generated by random amplified polymorphic DNA (RAPD) and isoenzyme electrophoresis analyses. Based on...
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PMID: 21175934
PDF is available here.
PDF is available here.
Abstract
Viral and prokaryotic abundance, production and diversity were determined throughout the water column of the subtropical Atlantic Ocean to assess potential variations in the relation between viruses and prokaryotes. Prokaryotic abundance and heterotrophic activity decreased by one and three orders o...
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PMID: 20485386
PDF is available here.
PDF is available here.
Abstract
Genetic analysis of 30 accessions of marvel grass (Dichanthium annulatum Forsk.), a tropical range grass collected from grasslands and open fields of drier regions, was carried out with the objectives of identifying unique materials that could be used in developing the core germplasm for such regions...
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PMID: 21506472
PDF is available here.
PDF is available here.
Biochemical Genetics (48)9-10 2010
Abstract
Fig, Ficus carica L., is a useful genetic resource for commercial cultivation. In this study, RAPD (60), ISSR (48), RAMPO (63), and SSR (34) markers were compared to detect polymorphism and to establish genetic relationships among Tunisian fig tree cultivars. The statistical procedures conducted on...
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PMID: 20628809
PDF is available here.
PDF is available here.
New Biotechnology (27)4 2010
Abstract
Fusarium wilt, caused by Fusarium oxysporum (Fo), is one of the most important fungal diseases worldwide. Like other plant pathogens, Fo displays specialized forms in association with its hosts. For example, F. oxysporum f. sp. niveum (Fon) is the damaging pathogen causing Fusarium w...
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PMID: 20471505
PDF is available here.
PDF is available here.
Abstract
Three strains of fructophilic lactic acid bacteria were isolated from flowers in South Africa. The isolates formed a subcluster in the Lactobacillus buchneri phylogenetic group, closely related to Lactobacillus fructivorans, Lactobacillus homohiochii, Lactobacillus lindneri and Lactobacillus sanfran...
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PMID: 19965998
PDF is available here.
PDF is available here.
Abstract
The present study was undertaken to evaluate genotoxic potential of two auxinic herbicides [2,4-dicholorophenoxy acetic acid (2,4-D) and 3,6-dichloro-2-methoxybenzoic acid (Dicamba)] in the roots of common bean (Phaseolus vulgaris L.) seedlings. Two-day-old etiolated seedlings were treated with 10 p...
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PMID: 20797789
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
We studied genomes of 22 cultivated flax varieties and 24 wild species from six sections of the genus Linum L. The chromosome numbers were exactly determined in the karyotypes of all studied species, and all individual chromosomes were identified by the C/DAPI-banding pattern and localization of 26S...
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PMID: 21254551
PDF is available here.
PDF is available here.
Abstract
Caryocar brasiliense (pequi), is one of the main species at the biome of the Brazilian savannah due to its use in culinary, popular medicine, industry in general, and iron and steel industry. At São José do Xingu (MT), a tree of C. brasiliense without thorn at the endocarp was found, which enables...
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PMID: 21562705
PDF is available here.
PDF is available here.
Food Microbiology (27)6 2010
Abstract
We selected representative and predominant strains as potential starter cultures for Hussuwa fermentation. These strains, L. fermentum strains BFE 2442 and BFE 2282 and P. acidilactici strain BFE 2300, were shown on the basis of RAPD-PCR fingerprinting to predominate in a model fermentation when use...
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PMID: 20630317
PDF is available here.
PDF is available here.
Journal of Parasitology (96)4 2010
Abstract
We used a PCR assay to obtain novel genome sequences from cercariae isolates of 3 European bird schistosome species ( Trichobilharzia franki , Trichobilharzia szidati , and Trichobilharzia regenti ) collected from freshwater ponds in Belorussia and Russia. We applied RAPD-fingerprinting using 1 rand...
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PMID: 20677938
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)8 2010
Abstract
Our result suggest effectiveness of AFLP** markers for characterization of the genetic structure and genetic relationships of the ginseng populations. These markers are recommended for use in large-scale population genetic studies of this species to develop measures of its conservation....
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PMID: 20873202
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)8 2010
Abstract
We estimated the genetic diversity within and among populations of wild Helianthus from five districts of Mozambique using RAPD markers. The 44 accessions studied grouped into four major clusters exhibiting structured variability with regard to geographic origin. A high level of genetic diversity (H...
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PMID: 20873205
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)8 2010
Abstract
The Giant river catfish, Sperata seenghala (Sykes) is commercially very important fish species of South Asia. Genetic variability between its populations collected from two rivers i.e. river Sutlej and river Beas of Indus river system in India were examined using randomly amplified polymorphic DNA a...
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PMID: 20873207
PDF is available here.
PDF is available here.
Abstract
In the last few decades, molecular typing has become an important tool in taxonomic, phylogenetic and identification studies of numerous groups of bacteria, including the yersiniae. In this study, Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR), Pulsed-Field Gel Electrophoresis (PFGE)...
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PMID: 20493215
PDF is available here.
PDF is available here.
Abstract
Cenchrus is an important component of major grass cover of world. Similar to the other major tropical grasses most of the species in genus Cenchrus are also apomictic in nature hence correct and precise identification of accessions and species are problematic and dubious. In the present study 187 de...
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PMID: 21186710
PDF is available here.
PDF is available here.
Current Microbiology (61)1 2010
Abstract
These results suggest that multidrug resistance of the strain M38100B may be caused by multiple mutations on its genomic DNA and a precursor stage for a homogeneous multidrug resistant population....
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PMID: 20039170
PDF is available here.
PDF is available here.
Abstract
Our observations suggest that DuCTLA-4 and its isoform DuCTLA-4DeltaTM evolved before the divergence of birds and mammals. Both DuCTLA-4 isoforms have significant structural homology to mammalian CTLA-4 proteins but their individual roles in the regulation of duck immune responses remains to be eluc...
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PMID: 20156479
PDF is available here.
PDF is available here.
Abstract
We then determined the full-length cDNA sequence of Sp-SPH, which contained 1298bp with an open reading frame of 1107bp encoding 369 amino acid residues. Multiple alignment analysis showed that the deduced amino acid sequences of Sp-SPH shared overall identity (83.8%) with those of SPH-containing pr...
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PMID: 20153768
PDF is available here.
PDF is available here.
Abstract
I crustins were both identified in CrusEs, suggesting that CrusEs is a new member of type I crustins. Quantitative real-time RT-PCR was employed to examine the expression of CrusEs, and its mRNA transcript was mainly detected in haemocytes and gill. The CrusEs mRNA transcript in haemocytes was down-...
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PMID: 20144896
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (98)1 2010
Abstract
A group of 67 Lactobacillus spp. strains containing Lactobacillus casei/paracasei, Lactobacillus fermentum, Lactobacillus gasseri, Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus salivarius species isolated from early childhood caries and identified to the species level in a previ...
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PMID: 20361257
PDF is available here.
PDF is available here.
Biochemical Genetics (48)5-6 2010
Molecular characterization of Fusarium oxysporum f. melongenae by ISSR and RAPD markers on eggplant.
Abstract
Fusarium oxysporum f. melongenae is a major soil-borne pathogen of eggplant (Solanum melongena). ISSR and RAPD markers were used to characterize Fusarium oxysporum f. melongenae isolates collected from eggplant fields in southern Turkey. Those isolates were not pathogenic to tomato. Pathogens were i...
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PMID: 20390339
PDF is available here.
PDF is available here.
Microbial Pathogenesis (48)6 2010
Abstract
The type III secretion system of Pseudomonas aeruginosa, responsible for acute infection, is composed of over twenty proteins that facilitate cytotoxin injection directly into host cells. Integral to this process is production and secretion of PcrV. Administration of a recently developed, anti-PcrV...
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PMID: 20211240
PDF is available here.
PDF is available here.
Abstract
We conducted a 6-year field experiment to investigate the effects of small-scale fragmentation on plant-pollinator interactions and genetic diversity in the self-compatible Betonica officinalis. We examined the abundance and composition of pollinators, the foraging behaviour of bumblebees and the pe...
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PMID: 20155288
PDF is available here.
PDF is available here.
Abstract
Among the Candida species causing bloodstream infections, Candida parapsilosis is one of the most frequently isolated. The objective of the present work was the identification of new microsatellite loci able to distinguish among C. parapsilosis isolates. DNA sequences with trinucleotide repeats were...
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PMID: 20220157
PDF is available here.
PDF is available here.
Abstract
To explore changes in the structure and diversity of activated sludge-derived microbial communities during adaptation to gradual increases in the concentration of wastewater, RAPD-PCR and the combination of PCR amplification of 16S rRNA genes with denaturing gradient gel electrophoresis (DGGE) analy...
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PMID: 20453903
PDF is available here.
PDF is available here.
Abstract
Abstract
Morphologically, nine different slow-growing protoclones were screened from regenerated protoplast of heterokaryotic Agaricus bisporus. The present study is the first report of fingerprinting on differentiating homo- and heterokaryotic protoclones using random amplified polymorphic DNA (RAPD) and in...
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PMID: 20467239
PDF is available here.
PDF is available here.
Abstract
Chuanniuxi (Radix Cyathule) is one of the most important geo-herb in Sichuan province, which adulterants are Hongniuxi (Cyathula capitata) and Huainiuxi (Achyranthes bidentata). In this paper Chuanniuxi and its adulterants were identified by SCAR markers. Nineteen populations from Tianquan, Baoxin,...
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PMID: 20617670
PDF is available here.
PDF is available here.
Mutation Research (697)1-2 2010
Abstract
The soil surface is a major natural system that accumulates pollutants and allows researchers to disclose the history and the present state of contamination of an area with toxic pollutants. This conclusion is based on the comparison of genotoxicity of aqueous extracts and DMSO extracts of topsoil f...
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PMID: 20152927
PDF is available here.
PDF is available here.
Abstract
A new test for pathogenic Leptospira isolates, based on RAPD-PCR and high-resolution melt (HRM) analysis (which measures the melting temperature of amplicons in real time, using a fluorescent DNA-binding dye), has recently been developed. A characteristic profile of the amplicons can be used to defi...
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PMID: 20406582
PDF is available here.
PDF is available here.
Abstract
To establish an effective way for rapid identification of Dendrobium candidum based on DNA molecular marker.
The genetic diversity among 11 wild species of Dendrobium was studied by using the random amplification polymorphism DNA. The special segment with random primer in Dendrobium candidum was rec...
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PMID: 20681295
PDF is available here.
PDF is available here.
Abstract
Biochemical Genetics (48)1-2 2010
Abstract
Biochemical Genetics (48)1-2 2010
Abstract
Genetic relationships were estimated among 24 accessions belonging to 11 species of Capsicum, using 2,760 RAPD markers based on touch-down polymerase chain reactions (Td-RAPD-PCR). These markers were implemented in analyses of principal coordinates, unweighted pair group mean average...
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PMID: 19916044
PDF is available here.
PDF is available here.
Biochemical Genetics (48)1-2 2010
Abstract
Random amplified polymorphic DNA markers were used to assess the genetic diversity within and among seven Tunisian diploid and polyploid populations of Teucrium polium L. from five bioclimatic areas. Out of the 141 bands generated from eight selected primers, 124 were polymorphic. Th...
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PMID: 20094842
PDF is available here.
PDF is available here.
Biochemical Genetics (48)1-2 2010
Abstract
Biochemical Genetics (48)1-2 2010
Abstract
Genetic relationships were estimated among 24 accessions belonging to 11 species of Capsicum, using 2,760 RAPD markers based on touch-down polymerase chain reactions (Td-RAPD-PCR). These markers were implemented in analyses of principal coordinates, unweighted pair group mean average...
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PMID: 19916044
PDF is available here.
PDF is available here.
Biochemical Genetics (48)1-2 2010
Abstract
Random amplified polymorphic DNA markers were used to assess the genetic diversity within and among seven Tunisian diploid and polyploid populations of Teucrium polium L. from five bioclimatic areas. Out of the 141 bands generated from eight selected primers, 124 were polymorphic. Th...
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PMID: 20094842
PDF is available here.
PDF is available here.
Biochemical Genetics (48)1-2 2010
Abstract
Biochemical Genetics (48)1-2 2010
Abstract
Genetic relationships were estimated among 24 accessions belonging to 11 species of Capsicum, using 2,760 RAPD markers based on touch-down polymerase chain reactions (Td-RAPD-PCR). These markers were implemented in analyses of principal coordinates, unweighted pair group mean average...
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PMID: 19916044
PDF is available here.
PDF is available here.
Biochemical Genetics (48)1-2 2010
Abstract
Random amplified polymorphic DNA markers were used to assess the genetic diversity within and among seven Tunisian diploid and polyploid populations of Teucrium polium L. from five bioclimatic areas. Out of the 141 bands generated from eight selected primers, 124 were polymorphic. Th...
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PMID: 20094842
PDF is available here.
PDF is available here.
Biochemical Genetics (48)1-2 2010
Abstract
Biochemical Genetics (48)1-2 2010
Abstract
Genetic relationships were estimated among 24 accessions belonging to 11 species of Capsicum, using 2,760 RAPD markers based on touch-down polymerase chain reactions (Td-RAPD-PCR). These markers were implemented in analyses of principal coordinates, unweighted pair group mean average...
|
PMID: 19916044
PDF is available here.
PDF is available here.
Biochemical Genetics (48)1-2 2010
Abstract
Random amplified polymorphic DNA markers were used to assess the genetic diversity within and among seven Tunisian diploid and polyploid populations of Teucrium polium L. from five bioclimatic areas. Out of the 141 bands generated from eight selected primers, 124 were polymorphic. Th...
|
PMID: 20094842
PDF is available here.
PDF is available here.
Biochemical Genetics (48)1-2 2010
Abstract
Biochemical Genetics (48)1-2 2010
Abstract
Genetic relationships were estimated among 24 accessions belonging to 11 species of Capsicum, using 2,760 RAPD markers based on touch-down polymerase chain reactions (Td-RAPD-PCR). These markers were implemented in analyses of principal coordinates, unweighted pair group mean average...
|
PMID: 19916044
PDF is available here.
PDF is available here.
Biochemical Genetics (48)1-2 2010
Abstract
Random amplified polymorphic DNA markers were used to assess the genetic diversity within and among seven Tunisian diploid and polyploid populations of Teucrium polium L. from five bioclimatic areas. Out of the 141 bands generated from eight selected primers, 124 were polymorphic. Th...
|
PMID: 20094842
PDF is available here.
PDF is available here.