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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Reverse Transcriptase Polymerase Chain Reaction (0):
Recent article
Reverse Transcriptase Polymerase Chain Reaction
Abstract
We have identified the high-affinity pyrimidine-preferring human concentrative nucleoside transporter (hCNT) 1 as the only hCNT-type protein expressed at both the basal and apical membranes. hCNT1 localization in lipid rafts is also dependent on its subcellular localization in the apical plasma memb...
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PMID: 21825094
PDF is available here.
PDF is available here.
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Reduction of isoprenaline-induced myocardial TGF-β1 expression and fibrosis in osthole-treated mice.
Abstract
We investigated the inhibitory effect of osthole on myocardial fibrotic formation in mice and its possible mechanisms. A mouse model with myocardial fibrosis was induced by hypodermic injection of isoprenaline while the mice were simultaneously treated with 40 and 80 mg/kg osthole for 40 days. After...
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PMID: 21854795
PDF is available here.
PDF is available here.
Developmental Cell (21)4 2011
Abstract
We show that Fgf8 and Sema3f, produced by early dental epithelium, respectively, attract and repulse mesenchymal cells, which cause them to pack tightly together during mouse tooth development. Resulting mechanical compaction-induced changes in cell shape induce odontogenic transcription factors (Pa...
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PMID: 21924961
PDF is available here.
PDF is available here.
Developmental Cell (21)4 2011
Abstract
We generated and characterized Atxn1L(-/-) and Atxn1(-/-); Atxn1L(-/-) mice. Atxn1L(-/-) mice have hydrocephalus, omphalocele, and lung alveolarization defects. These phenotypes are more penetrant and severe in Atxn1(-/-); Atxn1L(-/-) mice, suggesting that ATXN1 and ATXN1L are functionally redundan...
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PMID: 22014525
PDF is available here.
PDF is available here.
Developmental Cell (21)4 2011
Abstract
We identified an alternative mode of cytokinin action that uses endocytic trafficking as a means to direct plant organogenesis. This activity occurs downstream of known cytokinin receptors but through a branch of the cytokinin signaling pathway that does not involve transcriptional regulation. We sh...
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PMID: 21962902
PDF is available here.
PDF is available here.
Abstract
The present study aims to investigate the expression and significance of the anti-apoptotic gene Bag-1 in colorectal cancer and to evaluate the relationship between the gene and the disease.
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PMID: 21809033
PDF is available here.
PDF is available here.
Abstract
Gene expression profiles of paired hepatocellular carcinoma (HCC) and adjacent noncancerous liver tissue samples revealed preferential expression of midkine in HCC. This finding suggested the clinical usefulness of midkine measurement in serum for monitoring HCC treatment response, recurrence, and pr...
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PMID: 21917682
PDF is available here.
PDF is available here.
Abstract
We hypothesize that cell-scaffold interaction and mechanical deformation results in upregulation of genes related to osteogenic/chondrogenic differentiation and thus changes extracellular matrix (ECM) composition in human bone marrow mesenchymal stem cell (hBMSC)-derived tissue-engineered grafts. hB...
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PMID: 21554191
PDF is available here.
PDF is available here.
Abstract
We found previously that liver X receptor agonists, which regulate intracellular cholesterol homeostasis, augment PKA agonist- or high phosphate-induced osteogenic differentiation of vascular smooth muscle cells. Because cholesterol is an integral component of the matrix vesicles that nucleate calci...
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PMID: 21835914
PDF is available here.
PDF is available here.
Abstract
Dendritic cells (DCs) are critical in initiating immune responses by cross-priming of tumor Ags to T cells. Previous results showed that NK cells inhibited DC-mediated cross-presentation of tumor Ags both in vivo and in vitro. In this study, enhanced Ag presentation was observed in draining lymph nod...
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PMID: 21832159
PDF is available here.
PDF is available here.
Abstract
We aimed to clarify the underlying mechanisms of this cross talk. We found significantly higher titers of Ag-specific secretory IgA Ab in the vaginal wash after mucosal vaccination by both the intranasal (i.n.) and the intravaginal routes but not by the s.c. route. Interestingly, Ag-specific IgA Ab-...
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PMID: 21832166
PDF is available here.
PDF is available here.
Abstract
We present a mouse model in which a polyclonal sheep anti-mouse podocyte Ab caused subepithelial immune complex formation. Mice developed a nephrotic syndrome with severe edema, proteinuria, hypoalbuminemia, and elevated cholesterol and triglycerides. Development of proteinuria was biphasic: an init...
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PMID: 21844386
PDF is available here.
PDF is available here.
Abstract
We hypothesized that the NLRP3/caspase-1/IL-1β pathway is critical in DEP-induced lung inflammation. Upon DEP exposure, IL-1RI knockout mice had reduced pulmonary inflammation compared with wild-type mice. Similarly, treatment with rIL-1R antagonist (anakinra) and IL-1β neutralization impaired the...
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PMID: 21844393
PDF is available here.
PDF is available here.
Abstract
We report on a novel, highly sensitive IL-10 reporter mouse based on the reporter enzyme β-lactamase and the fluorescence resonance energy transfer substrate coumarin-cephalosporin-fluorescein (4). In contrast to an IL-10 reporter mouse model that we generated by using enhanced GFP as reporter and...
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PMID: 21844394
PDF is available here.
PDF is available here.
Abstract
We hypothesized that recombinant activated protein C would also suppress SLE and lupus nephritis. To test this concept, autoimmune female MRL-Fas(lpr) mice were injected with either vehicle or recombinant human activated protein C from week 14-18 of age. Activated protein C treatment significantly s...
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PMID: 21849682
PDF is available here.
PDF is available here.
Abstract
We found that Treg act on the developing immune response early postinfection by reducing the extent of dendritic cell costimulatory molecule expression. Due to this change and the lower IL-2 production that results, a substantial fraction of CD8(+) effector T cells lose CD25 expression several days...
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PMID: 21849683
PDF is available here.
PDF is available here.
Abstract
We report that CD86 is ubiquitinated in DCs via MARCH1 E3 ubiquitin ligase and that this ubiquitination plays a key role in CD86 regulation. Ubiquitination at lysine 267 played the most critical role for this regulation. CD86 is ubiquitinated in MARCH1-deficient DCs to a much lesser degree than in w...
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PMID: 21849678
PDF is available here.
PDF is available here.
Critical Care Medicine (39)9 2011
Abstract
We have recently shown that MFG-E8 treatment attenuates organ injury, inflammatory responses, and survival after sepsis through the enhancement of phagocytosis of apoptotic cells. The purpose of this study was to determine whether administration of MFG-E8 attenuates renal ischemia-reperfusion injury...
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PMID: 21666453
PDF is available here.
PDF is available here.
Critical Care Medicine (39)9 2011
Abstract
We have recently shown that MFG-E8 treatment attenuates organ injury, inflammatory responses, and survival after sepsis through the enhancement of phagocytosis of apoptotic cells. The purpose of this study was to determine whether administration of MFG-E8 attenuates renal ischemia-reperfusion injury...
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PMID: 21666453
PDF is available here.
PDF is available here.
Abstract
We examined the effect of PHD inhibition on the expression of angiotensin II type 1 receptor (AT(1)R). Hypoxia, cobalt chloride, and dimethyloxalylglycine, all known to inhibit PHD, reduced AT(1)R expression in vascular smooth muscle cells. Knockdown of PHD2, a major isoform of PHDs, by RNA interfer...
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PMID: 21825224
PDF is available here.
PDF is available here.
Abstract
Our results demonstrate that the levels of MMP-9 are increased in the heart of mdx mice. Genetic ablation of MMP-9 attenuated cardiac injury, left ventricle dilation, and fibrosis in 1-y-old mdx mice. Echocardiography measurements showed improved heart function in Mmp9-deficient mdx mice. Deletion o...
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PMID: 21810612
PDF is available here.
PDF is available here.
Abstract
We show that in both RAW264.7 macrophage cells and primary bone marrow-derived macrophages, the production of IFN-β and IL-6, but not TNF, in response to cyclic-di-AMP and cyclic-di-GMP requires MPYS (also known as STING, MITA, and TMEM173). Furthermore, expression of MPYS was required for IFN resp...
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PMID: 21813776
PDF is available here.
PDF is available here.
Abstract
We have engineered genetic ablation of Pax7(+) cells to test whether there is any significant contribution to muscle regeneration after acute injury from cells other than this source. We find that such elimination of Pax7(+) cells completely blocks regenerative myogenesis either following injury to...
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PMID: 21828092
PDF is available here.
PDF is available here.
Abstract
We previously reported that the class 3 semaphorin SEMA3A signals through its neuropilin receptors, NRP1 and NRP2, to organise the axons that guide migrating GnRH neurons from their birthplace into the brain. By combining analysis of genetically altered mice with in vitro models, we show here that t...
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PMID: 21828096
PDF is available here.
PDF is available here.
Abstract
We isolated a novel Drosophila male sterile mutant, garmcho (garm). Positional cloning indicated that garm encodes a subunit of the Drosophila THO complex, THOC5. Flies lacking THOC5 showed a meiotic arrest phenotype with severe nucleolar disruption in primary spermatocytes. A functional GFP-tagged...
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PMID: 21828100
PDF is available here.
PDF is available here.
Abstract
We previously reported that it also plays a key role in the tumorigenesis and metastasis of human osteosarcoma. In the present study, we constructed a recombinant encoding BLCAP cDNA. Overexpression of BLCAP resulted in growth inhibition and induced apoptosis of human TC-135 Ewing's sarcoma cells in...
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PMID: 21844121
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
These results highlight the fact that testing of properly frozen OP samples could add value to the understanding of the epidemiology and ecology of AIV in waterfowl populations....
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PMID: 22017043
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
We used real-time reverse transcriptase-polymerase chain reaction to evaluate the expression profiling of a panel of chicken genes in rMd5- and rMd5deltameq-infected chickens at 5, 14, 21, and 35 days postinfection (dpi). Although the transcriptional activities of several immune-related genes, inclu...
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PMID: 22017031
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
We present a novel detection and genotyping method based on the simultaneous characterization of both IBDV genomic segments followed by a simple restriction fragment length polymorphism (RFLP) assay. This single restriction enzyme, multiplex reverse transcriptase-PCR/RFLP diagnostic test not only di...
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PMID: 22017032
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
The very virulent form of infectious bursal disease virus (vvIBDV) causes an immunosuppressive disease that is further characterized by the rapid onset of morbidity and high mortality in susceptible chickens. In 2009, vvIBDV was first reported in California, U. S. A., and since that...
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PMID: 22017037
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
An avian influenza virus (AIV), A/Emu/Israel/552/2010/(H5N1), was isolated from a dead emu that was found in the Ein Gedi oasis near the Dead Sea. The virus molecular characterization was performed by reverse transcriptase-polymerase chain reaction (RT-PCR) and real-time RT-PCR using...
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PMID: 22017055
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
Tracheal and cloacal swabs as well as blood samples from 408 feral urban (Columba livia forma domestica) and 170 free-ranging wood pigeons (Columba palumbus) in Germany were tested for infection with avian influenza viruses (AIVs). Neither influenza A virus was isolated in the swab s...
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PMID: 22017045
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
Between 2006 and 2009, seven strains of infectious bronchitis (IB) virus (IBV) were isolated from vaccinated chicken flocks on different chicken farms in China. The pathogenic characters of seven IBV strains were assessed. Each of the seven strains was infective to the test chickens...
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PMID: 22017046
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
Infectious bronchitis virus (IBV) Cal99 variant was isolated from the kidneys of seven 2-5-mo-old game chickens with nephritis and respiratory disease. IBV Cal99 variant is usually associated with respiratory disease in broiler chickens in California. Macroscopically, the majority of...
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PMID: 22017040
PDF is available here.
PDF is available here.
Journal of Immunology (187)5 2011
Abstract
We demonstrated that STAT3 negatively regulates type I IFN-mediated response. STAT3 knockdown or knockout cells displayed enhanced gene expression and antiviral activity in response to IFN-α/β. Restoration of STAT3 to STAT3KO cells resulted in attenuation of the response. Upon viral infection, inc...
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PMID: 21810606
PDF is available here.
PDF is available here.
Development (138)17 2011
Abstract
We generated a model for the conditional depletion of satellite cells by expressing a human diphtheria toxin receptor under control of the murine Pax7 locus. Intramuscular injection of diphtheria toxin during muscle homeostasis, or combined with muscle injury caused by myotoxins or exercise, led to...
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PMID: 21828093
PDF is available here.
PDF is available here.
Abstract
Parvoviruses are small, 260-A-diameter, icosahedral, non-enveloped, single-stranded DNA viruses with a genome of approximately 5 kb. Non structural protein, (NS-1) is especially relevant, being both essential for virus replication and the main factor responsible for virus pathogenici...
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PMID: 21941937
PDF is available here.
PDF is available here.
Abstract
Coronary artery disease (CAD) is a common and multifactorial arterial disease that is mainly caused by atherosclerosis. Macrophages, lymphocytes and neutrophils have been implicated in atherosclerotic plaque development. Autophagy, a highly conserved cellular process for the removal...
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PMID: 21812771
PDF is available here.
PDF is available here.
Abstract
We concluded that ANG II stimulates directly and indirectly (via sympathostimulator pathway) the MMP-2 expression but seems unable to affect MMP-9 expression through direct pathway. Combined inhibition of SNS and ANG II were more efficient than a single inhibition in reducing MMP amounts in rat vess...
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PMID: 21952436
PDF is available here.
PDF is available here.
Abstract
We have examined possible roles of these genes in mycobacterial cell wall biosynthesis through inactivation of their respective orthologs in Mycobacterium smegmatis mc(2)155, phenotypic characterization of the mutant strains via metabolic labeling with [U-(14)C]-glucose, cell-free reactions with UDP...
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PMID: 21952433
PDF is available here.
PDF is available here.
Abstract
We also observed that, similarly to SRIF, the specific knockdown of CaMKIIβ induces a decrease of both GH content/secretion. These results raise the hypothesis that CaMKIIβ may mediate, at least in part, the SRIF-induced control of [Ca(2+)](i). In addition, CaMKIIβ seems to play a positive role i...
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PMID: 21952434
PDF is available here.
PDF is available here.
Abstract
We describe the main clinical signs and the laboratory results associated with the detection of a betanodavirus in hybrid striped bass x white bass (Morone saxatilis x Morone chrysops) and largemouth bass Micropterus salmoides, reared in a freshwater environment. We also detected the virus by real-t...
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PMID: 21991664
PDF is available here.
PDF is available here.
Abstract
These results indicate that estradiol and progesterone act in a synergistic manner to upregulate MMTV, which subsequently induces breast cancer in TA2 mice. Various members of the Wnt gene family may play specific roles in different stages of carcinogenesis in TA2 mice....
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PMID: 21852035
PDF is available here.
PDF is available here.
Genes & Development (25)16 2011
Abstract
We have determined the cistrome and transcriptome for the nuclear receptor liver receptor homolog-1 (LRH-1) in exocrine pancreas. Chromatin immunoprecipitation (ChIP)-seq and RNA-seq analyses reveal that LRH-1 directly induces expression of genes encoding digestive enzymes and secretory and mitochon...
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PMID: 21852532
PDF is available here.
PDF is available here.
British Journal of Cancer (105)4 2011
Abstract
Chronic treatment of SLK cells with one of the five HIV-PIs alone or together resulted in increased resistance to doxorubicin. Co-treatment with one of the HIV-PIs in combination with doxorubicin resulted in a synergistic increase in resistance to doxorubicin, and the degree of resistance was found t...
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PMID: 21829205
PDF is available here.
PDF is available here.
Abstract
Atherosclerosis is a chronic inflammatory process. Poly(ADP-ribose) polymerase-1 (PARP), a nuclear enzyme linked to DNA repair, has been shown to be involved in atherogenesis; however, the effects on dendritic cells, T cells and serum auto-antibody levels are not fully understood....
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PMID: 21813379
PDF is available here.
PDF is available here.
Abstract
We found that VPA potently induced hyperacetylation of histone H3 and H4, increased the expression of E-cadherin and inhibited cell migration in prostate cancer cells. Furthermore, knock-down of E-cadherin significantly restored the effects of VPA on cell migration, while over-expression of E-cadher...
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PMID: 21901986
PDF is available here.
PDF is available here.
Abstract
Changes in gene expression of beta1- and beta2-adrenoceptors (beta1 - and beta2-AR) in right and left atria and ventricles after fluoxetine treatment in stress-induced depression of adult rat males were studied. Elevated beta1-AR mRNA levels in the left atria and significantly higher...
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PMID: 21901985
PDF is available here.
PDF is available here.
Abstract
Leptin contributes to the pathogenesis of atherosclerosis. Ang II (angiotensin II), a proatherogenic cytokine, increases leptin synthesis in cultured adipocytes. Statin suppresses leptin expression in adipocytes and human coronary artery endothelial cells. However, the effect of Ang II and statin on...
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PMID: 21806545
PDF is available here.
PDF is available here.
Abstract
Bone marrow has been the elected cell source of studies published so far concerning bone and cartilage tissue-engineering approaches. Recent studies indicate that adipose tissue presents significant advantages over bone marrow as a cell source for tissue engineering. Most of these st...
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PMID: 21268288
PDF is available here.
PDF is available here.
Abstract
We performed a quantitative gene expression analysis of well-defined differentiation makers: myogenic factor 5 (MYF5), myogenin (MYOG), skeletal muscle αactin1 (ACTA1), embryonic (MYH3), perinatal (MYH8) and adult skeletal muscle myosin heavy chain (MYH1). Additionally, the fusion indices of formin...
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PMID: 21370490
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1812)8 2011
Abstract
We identified a hitherto unreported compound heterozygous mutation del exon2-3/del exon3 in the parkin gene, leading to the complete loss of the full-length protein. In both patients, but not in their heterozygous parental control, we observed severe ultrastructural abnormalities, mainly in mitochon...
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PMID: 21215313
PDF is available here.
PDF is available here.
Life Sciences (89)5-6 2011
Abstract
We measured concentrations of LPA and its precursor, lysophosphatidylcholine stored in the urinary bladder and present in the swollen pelvis of the ligated kidney as well as the corresponding blood plasma by liquid chromatography-tandem mass spectrometry.
We found that concentrations...
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PMID: 21693124
PDF is available here.
PDF is available here.
Life Sciences (89)5-6 2011
Abstract
We measured concentrations of LPA and its precursor, lysophosphatidylcholine stored in the urinary bladder and present in the swollen pelvis of the ligated kidney as well as the corresponding blood plasma by liquid chromatography-tandem mass spectrometry.
We found that concentrations...
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PMID: 21693124
PDF is available here.
PDF is available here.
Life Sciences (89)5-6 2011
Abstract
We measured concentrations of LPA and its precursor, lysophosphatidylcholine stored in the urinary bladder and present in the swollen pelvis of the ligated kidney as well as the corresponding blood plasma by liquid chromatography-tandem mass spectrometry.
We found that concentrations...
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PMID: 21693124
PDF is available here.
PDF is available here.
Abstract
To describe the clinical manifestations of patients affected with a novel influenza A (H1N1 2009) during the pandemic.
A retrospective study was conducted in patients with influenza-like illness receiving care at Chiang Mai University Hospital between June 1 and Septe...
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PMID: 21863671
PDF is available here.
PDF is available here.
Abstract
The present study was undertaken to clone, express rabies virus glycoprotein (RVG) and to identify potential T-cell epitopes on it. RVG gene (1590 bp) was amplified using gene specific primers. The amplified product was cloned into pTZ57R/T cloning vector by TA cloning. RVG gene was...
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PMID: 21870427
PDF is available here.
PDF is available here.
Abstract
Beta-arrestin 2 has been shown to participate in the pathogenesis of asthma by inducing Th2 cell migration to the lungs. Whether beta-arrestin 2 regulates cytokine production of CD4+ T cells is still unknown. The aim of the present study was to investigate the effect of beta-arrestin...
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PMID: 21870426
PDF is available here.
PDF is available here.
Abstract
We measured the insulin sensitivity index and determined the GLUT4 protein and mRNA contents of skeletal muscle by Western blotting and reverse transcription-polymerase chain reaction (RT-PCR), respectively, in normal people and liver cirrhosis patients. The results showed that the levels of glucose...
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PMID: 21796809
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1811)10 2011
Abstract
StarD4 is a member of the StarD4 subfamily of START domain proteins with a characteristic lipid binding pocket specific for cholesterol. The objective of this study was to define StarD4 subcellular localization, regulation, and function. Immunobloting showed that StarD4 is highly expressed in the mou...
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PMID: 21767660
PDF is available here.
PDF is available here.