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Polymerase Chain Reaction (4):
Recent article
Polymerase Chain Reaction
Orvosi Hetilap (152)45 2011
Abstract
Incidence of the JAK2 mutation in their patients with chronic myeloproliferative neoplasia subgroups mainly corresponds to the literary data. Thrombosis ensued both in JAK positive polycythemia vera and essential thrombocythemia cases occurred nearly in the same number, but the incidence of thrombos...
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PMID: 22011365
PDF is available here.
PDF is available here.
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Orvosi Hetilap (152)45 2011
Abstract
The results suggest that the prevalence of HPV in this study population exceeds the international data. The results attracts the attention the peak prevalence of the high risk types in the youngest age-group, and the higher risk of cervical abnormality in case of presence of two or more HPV types. T...
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PMID: 22011366
PDF is available here.
PDF is available here.
Abstract
We first conducted 4 association studies (2639 individuals) that showed a protective effect of the -308A allele (odds ratio [OR] = 0.77; P = .005). Next, results of a meta-analysis reinforced this association after inclusion of our new data (OR = 0.74; P = .04). Furthermore, a subgroup analysis incl...
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PMID: 21917899
PDF is available here.
PDF is available here.
Abstract
We demonstrate here the identification and phylogenetic characterization of Babesia microti (B. microti)-like parasite detected from a splenectomized Japanese macaque (Macaca fuscata fuscata) at a facility for laboratory animal science. On Day 133 after splenectomy, intra-erythrocytic parasites were...
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PMID: 21976563
PDF is available here.
PDF is available here.
Abstract
These results suggest the potential defined antigens for development of an accurate diagnostic test for schistosomiasis....
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PMID: 21976571
PDF is available here.
PDF is available here.
Abstract
We conducted a survey to describe infection prevalence of tick-borne pathogens in Amblyomma americanum and Dermacentor variabilis ticks collected in Kentucky. During 2007-2008, we collected 287 ticks (179 D. variabilis and 108 A. americanum) from canine, feral hog, horse, raccoon, white-tailed deer,...
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PMID: 21976578
PDF is available here.
PDF is available here.
Abstract
Abstract. Outbreaks of Mayaro fever have been associated with a sylvatic cycle of Mayaro virus (MAYV) transmission in South America. To evaluate the potential for a common urban mosquito to transmit MAYV, laboratory vector competence studies were performed with Aedes aegypti from Iquitos, Peru. Oral...
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PMID: 21976583
PDF is available here.
PDF is available here.
Abstract
We developed a luciferase-based assay system for measuring the frequency of chromosomal homologous recombination and SSA. An I-SceI-induced DSB, within a nonfunctional luciferase gene, could be efficiently repaired by HR. Additionally, the continuous expression of the I-SceI endonuclease in the HR r...
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PMID: 21842267
PDF is available here.
PDF is available here.
Abstract
We present a systematic review of published microsatellite primer notes and show that, on average, about half of all candidate loci are lost due to insufficient PCR amplification, monomorphism or multicopy status in the genome, no matter what isolation strategy is used. Thus, the screening of candid...
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PMID: 21847526
PDF is available here.
PDF is available here.
Abstract
The dipteran Chironomus riparius is found across the entire Palearctic region; its larvae are among the most abundant macroinvertebrates inhabiting inland waterbodies. Chironomid larvae have been extensively used in ecotoxicological and cytogenetic research, but relatively little is...
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PMID: 21901555
PDF is available here.
PDF is available here.
Abstract
We investigated the usefulness of cytologic direct smears as an alternative specimen source for mutation testing. Tumor cell-enriched areas from freshly prepared and archived rapid Romanowsky-stained direct smears in 33 cases of lung carcinoma were microdissected for DNA isolation and evaluated for...
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PMID: 21917678
PDF is available here.
PDF is available here.
Abstract
The ATHENA (Addressing THE Need for Advanced HPV Diagnostics) HPV study evaluated the clinical usefulness of the cobas HPV Test (Roche Molecular Systems, Pleasanton, CA) for high-risk human papillomavirus (HR-HPV) testing (14 HR types) and individual HPV-16/HPV-18 genotyping in women undergoing routi...
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PMID: 21917680
PDF is available here.
PDF is available here.
Ideggyogyaszati szemle (64)9-10 2011
Abstract
We summarize those epilepsies, in which genetic testing has clinical significance. Different types of genetic tests are presented. Na-channel epilepsies include different clinical entities, the exact genetic diagnosis is relevant in the prognosis, genetic counseling, as well in the therapeutic decis...
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PMID: 22059368
PDF is available here.
PDF is available here.
Abstract
We identify a promising targeted therapeutic strategy for two Ras-driven cancers: Nf1-deficient malignancies and Kras/p53 mutant lung cancer. We show that agents that enhance proteotoxic stress, including the HSP90 inhibitor IPI-504, induce tumor regression in aggressive mouse models, but only when...
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PMID: 21907929
PDF is available here.
PDF is available here.
Abstract
We report that there is a biased usage of TCR Vβ6.7 chain among TCRs reactive to the immunodominant DQ2-α-II gliadin epitope. We isolated Vβ6.7 and DQ2-αII tetramer-positive CD4(+) T cells from peripheral blood of gluten-challenged celiac patients and sequenced the TCRs of a large number of sing...
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PMID: 21849672
PDF is available here.
PDF is available here.
Abstract
We tested 10 diagnostic assays, including 1 commercial real-time polymerase chain reaction (qPCR) test for the laboratory detection of toxigenic C difficile on 1,000 stool samples. Sensitive culture for toxigenic C difficile using 2 types of media with broth enrichment defined the reference standard...
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PMID: 21846912
PDF is available here.
PDF is available here.
Abstract
We examine the role of S. aureus virulence factors for gut colonization. S. aureus isolated from quantitative stool cultures of 49 Swedish infants followed from birth to 12 months of age were assessed for 30 virulence-associated genes, spa type, and agr allele by serial polymerase chain reaction (PC...
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PMID: 21844297
PDF is available here.
PDF is available here.
Abstract
We found 117 type-specific HPV infections from the HIV-positive individuals, including 73 α-HPV, 33 β-HPV, and 11 γ-HPV infections; whereas, the distribution was 46 α-HPV, 108 β-HPV, and 14 γ-HPV infections from 168 type-specific infections from the 317 male participants. Conclusions. The or...
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PMID: 21844305
PDF is available here.
PDF is available here.
Abstract
We found that after injury satellite cells and fibroblasts rapidly proliferate in close proximity to one another. To test the role of satellite cells and fibroblasts in muscle regeneration in vivo, we created Pax7(CreERT2) and Tcf4(CreERT2) mice and crossed these to R26R(DTA) mice to genetically abl...
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PMID: 21828091
PDF is available here.
PDF is available here.
Abstract
These results provide convincing evidence that skeletal muscle fibers are capable of mounting a robust hypertrophic response to mechanical overload that is not dependent on satellite cells....
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PMID: 21828094
PDF is available here.
PDF is available here.
Abstract
KIT, a transmembrane receptor tyrosine kinase, is one of the specific targets for anti-cancer therapy. In humans, its expression and mutations have been identified in malignant melanomas and therapies using molecular-targeted agents have been promising in these tumours. As human malignant melanoma, c...
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PMID: 21848624
PDF is available here.
PDF is available here.
Abstract
Abstract
Expressing proteins of interest as fusion to proteins of bacterial envelope is a powerful technique for biotechnological and medical applications. The synthetic gene (VacII) encoding for T-cell epitopes of selected genes of Mycobacterium tuberculosis namely, ESAT6, MTP40, 38 kDa, and...
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PMID: 21941936
PDF is available here.
PDF is available here.
Abstract
These results were confirmed by direct DNA sequencing and by high-resolution melting analysis. The costs and time constraints of each detection method were compared at the same time. In conclusion, our newly designed multiplex SNaPshot assay is a fast, inexpensive, sensitive, and robust technique fo...
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PMID: 21742054
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
Toll-like receptors (TLRs), a family of transmembrane and cytosolic proteins, detect microbial patterns, initiating innate immune responses in various organisms. Although they are abundant, genetic characterization and functional differences of TLRs in economically important avian sp...
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PMID: 22017051
PDF is available here.
PDF is available here.
Avian Diseases (55)3 2011
Abstract
An outbreak of Mycobacterium avium subsp. avium infection was diagnosed in one breed of domestic pigeons (Columba livia f. domestica) in the Czech Republic. Nodular granulomatous lesions were found in 42 (9.7%) pigeons of the 435 examined; histopathologic examination of livers with g...
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PMID: 22017056
PDF is available here.
PDF is available here.
Abstract
Hexose-6-phosphate dehydrogenase (H6PD) is the main NADPH generating enzyme in the lumen of the endoplasmic reticulum. H6PD is regarded as an ancillary enzyme in prereceptorial glucocorticoid activation and probably acts as a nutrient sensor and as a prosurvival factor. H6PD expression was determined...
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PMID: 21620971
PDF is available here.
PDF is available here.
Molecular features of colorectal hyperplastic polyps and sessile serrated adenoma/polyps from Korea.
Abstract
We conclude that HPs and SSA/Ps in Korean patients share some, but not all, clinical and molecular characteristics to those that occur in Americans. The data support the theory that the right and left colon are biologically different with regard to susceptibility to serrated cancer, and that anatomi...
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PMID: 21836485
PDF is available here.
PDF is available here.
Molecular Immunology (48)15-16 2011
Abstract
We investigated gene regulation at the IL-3/GM-CSF gene cluster. We found BRG1, a SWI/SNF remodeling ATPase, bound a distal element, CNSa. BRG1 binding was strongest in differentiated, stimulated T helper cells, paralleling IL-3 and GM-CSF expression. Depletion of BRG1 reduced IL-3 and GM-CSF transc...
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PMID: 21831442
PDF is available here.
PDF is available here.
Journal of Cell Biology (194)3 2011
Abstract
We hypothesize that genes relevant for cardiac proliferation are targeted to heterochromatin by retinoblastoma (Rb) family members interacting with E2F transcription factors and recruiting heterochromatin protein 1 (HP1) proteins. To test this hypothesis, we created cardiac-specific Rb and p130 indu...
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PMID: 21825075
PDF is available here.
PDF is available here.
Abstract
Noninvasive prenatal determination of fetal sex using cell-free fetal DNA provides an alternative to invasive techniques for some heritable disorders. In some countries this testing has transitioned to clinical care, despite the absence of a formal assessment of performance....
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PMID: 21828326
PDF is available here.
PDF is available here.
Abstract
We show here a study on the pathogenesis and the physiopathology of ROCV disease in the central nervous system of a Balb/C young adult mice experimental model. The animals were intraperitoneally infected by ROCV and followed from 0 to 9 days after infection, when all of them died. Nervous tissue sam...
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PMID: 21813860
PDF is available here.
PDF is available here.
Abstract
Delivery of a high ratio of plasma to packed red blood cells to patients who require massive transfusion is associated with improved survival. Hemorrhagic shock causes increased production of pro-inflammatory cytokines. These are associated with late morbidity and mortality. The use of fresh frozen p...
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PMID: 21825929
PDF is available here.
PDF is available here.
Abstract
To determine the association of high-risk alleles in the complement factor H (CFH; Y402H, rs1061170) and age-related maculopathy susceptibility (ARMS2; A69S, rs10490924) genes with reticular macular disease (RMD), a major clinical subphenotype of age-related macular degeneration (AMD).
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PMID: 21825189
PDF is available here.
PDF is available here.
Abstract
Abstract
Ehrlichiosis is a clinically important, emerging zoonosis. Only Ehrlichia chaffeensis and E. ewingii have been thought to cause ehrlichiosis in humans in the United States. Patients with suspected ehrlichiosis routinely undergo testing to ensure proper diagnosis and to ascertain the cause.
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PMID: 21812671
PDF is available here.
PDF is available here.
Abstract
Characterization of features of influenza pandemic development in Russia in relation to global process.
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PMID: 21809641
PDF is available here.
PDF is available here.
Abstract
To study the protocol of construction of a KCNQ2-c.812G>T mutant and it's eukaryotic expression vector, the c.812G>T (p.G271V) mutation which was detected in a Chinese pedigree of benign familial infantile convulsions, and to examine the expression of mutant protein in human embyonic kidney (HEK) 293...
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PMID: 21849107
PDF is available here.
PDF is available here.
Abstract
The results of intraspecies heterogeneity analysis of 4 bifidobacterial species, B. longum, B. animalis subsp. lactics, B. bifidum and B. dentium colonizing intestines of people of different age groups are presented in the paper. A high level of intraspecies variability has been revealed for all spec...
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PMID: 21809682
PDF is available here.
PDF is available here.
Abstract
Abstract
γδ T-cell large granular lymphocytic (T-LGL) leukemia of the CD4-/CD8- subtype is rare, and data are limited in the literature. This study evaluated the clinical, morphologic, immunophenotypic, and molecular cytogenetic features of 7 cases of CD4-/CD8- γδ T-LGL leukemia. Although...
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PMID: 21757603
PDF is available here.
PDF is available here.
Abstract
We have generated type 1 diabetic Akita mice lacking the low density lipoprotein receptor (Ins2(Akita)Ldlr(-/-)). Ins2(Akita)Ldlr(-/-) mice were severely hyperglycemic with impaired glucose tolerance. Compared with Ldlr(-/-) mice, 20-week-old Ins2(Akita)Ldlr(-/-) mice fed a 0.02% cholesterol AIN76a...
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PMID: 21606463
PDF is available here.
PDF is available here.
Abstract
To investigate the possible influence of tumour necrosis factor-alpha (TNF), TNF receptor I (TNFRI) and TNF receptor II (TNFRII) gene polymorphisms on anti-TNF treatment responsiveness, stratified by autoantibody status.
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PMID: 21813066
PDF is available here.
PDF is available here.
Abstract
The cytochrome P450 (CYP) 2C19*2 loss-of-function allele has been associated with impaired clopidogrel response and worse prognosis in clopidogrel-treated patients. The benefit of tailored therapy according to platelet function test results remains unclear, and the potential effect o...
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PMID: 21803320
PDF is available here.
PDF is available here.
Abstract
We tested whether circulating miRNAs in the plasma of critically ill patients with acute kidney injury (AKI) at the inception of renal replacement therapy are deregulated and may predict survival.
We profiled miRNAs using RNA isolated from the plasma of patients with AKI and healthy...
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PMID: 21700819
PDF is available here.
PDF is available here.
Abstract
We analyzed TRPC6 in 33 Italian children with sporadic early-onset SRNS and three Italian families with adult-onset FSGS. Design, setting, participants, & measurements TRPC6 mutation analysis was performed through PCR and sequencing. The effects of the detected amino acid substitutions were analyzed...
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PMID: 21734084
PDF is available here.
PDF is available here.
Abstract
We compared the performance of Xpert MTB/RIF, MTBDRplus (Hain Lifescience), LightCycler Mycobacterium Detection (LCTB) (Roche), with acid fast bacilli (AFB) smear microscopy and liquid culture on a single sputum specimen....
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PMID: 21814495
PDF is available here.
PDF is available here.
Abstract
We examined a genetic marker on the CD86 gene in kidney transplant outcome.
In our retrospective study, 168 kidney allograft recipients were genotyped by direct sequencing. Patients were classified into 2 groups of 29 human leukocyte antigen (HLA)-identical haplotype allograft recipi...
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PMID: 21525579
PDF is available here.
PDF is available here.
Abstract
We analyzed the whole internal bacterial community of natural populations of D. gallinae originating from two types of poultry farm habitats (standard and free-range) in two regions of France (Brittany and the Rhone-Alpes). Total DNA was extracted from individual or pooled mites, and polymerase chai...
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PMID: 21845937
PDF is available here.
PDF is available here.
Abstract
These results indicated that infected birds with avian Plasmodium inhabited and direct contacts occurred between the infected birds and mosquitoes in Kushiro Wetland, Hokkaido, Japan....
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PMID: 21845952
PDF is available here.
PDF is available here.
Abstract
The feeding patterns of haematophagous arthropods are of major importance in the amplification and transmission of infectious disease agents to vertebrate hosts, including humans. The establishment of new vector populations in nonnative range might alter transmission networks. The Asian tiger mosquit...
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PMID: 21845962
PDF is available here.
PDF is available here.
New Microbiologica (34)3 2011
Abstract
Multiple sclerosis (MS) is the most common autoimmune disease characterized by multifocal areas of inflammatory demyelination within the central nervous system. Cytomegalovirus (CMV) has a complex pathobiology and in most cases is simply asymptomatic. There is some recent controversy over the role of...
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PMID: 21811746
PDF is available here.
PDF is available here.
Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG / World Association of Sarcoidosis and Other Granulomatous Disorders (28)1 2011
The investigation of HLA microsatellites influence in predisposition to sarcoidosis among Croatians.
Abstract
I loci (A, B) by serology, while PCR-SSP method was used for HLA class II loci (DRB1, DQB1). Five HLA microsatellites were analyzed by PCR and electrophoresis in an automated sequencer. No significant deviation in the distribution of frequencies at HLA class I alleles was observed between the two pa...
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PMID: 21796887
PDF is available here.
PDF is available here.
Oral Oncology (47)9 2011
Abstract
Nasopharyngeal carcinoma (NPC) is a solid tumor closely associated with Epstein-Barr virus (EBV) infection. The purpose of this investigation was to detect and quantify the EBV DNA level in salivary samples of NPC patients following treatment using real-time PCR. A total of 175 consecutive newly diag...
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PMID: 21767975
PDF is available here.
PDF is available here.
Abstract
Geomyces destructans produces the white fungal growth on the muzzle and the tacky white discoloration on wings and ears that characterize white-nose syndrome (WNS) in cave-hibernating bats. To test the hypothesis that postemergent WNS-infected bats recover from infection with G. destructans, 30 littl...
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PMID: 21719826
PDF is available here.
PDF is available here.
Abstract
The study was targeted to investigate the propagation of rubella virus in the cell cultures of various origins and with different cultivation methods. The high-yielding strain of rubella virus was produced. The "spinner-culture" cultivation method was applied and the strain's RNA was...
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PMID: 21899118
PDF is available here.
PDF is available here.
Abstract
Indoor air quality has become a critical issue because people spend most of their time in the indoor environment. The factors that influence indoor air quality are very important to environmental sanitation and air quality improvement. This study focuses on monitoring air quality, co...
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PMID: 21850827
PDF is available here.
PDF is available here.
Abstract
To evaluate the effectiveness of the flexible bronchoscopy in the diagnosis and treatment of refractory pneumonia among children.
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PMID: 21752319
PDF is available here.
PDF is available here.
Abstract
To determine the accuracy of alpha-thal immunochromatographic (IC) strip in diagnosis of alpha-thalassemia 1 carrier among pregnant women, using PCR for alpha-thalassemia 1 (SEA type) as a gold standard.
Asymptomatic pregnant women attending the antenatal care clinic...
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PMID: 21774279
PDF is available here.
PDF is available here.
Abstract
We collected 129 FFPE specimens, including 56 nontuberculosis cases, 63 MTB cases, and 10 nontuberculous mycobacteria (NTM) cases determined by acid-fast bacilli (AFB) culture. We performed AFB staining; nested MTB PCR, targeting the IS6110 gene; and real-time MTB PCR, targeting the senX3-regX3 inte...
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PMID: 21704272
PDF is available here.
PDF is available here.