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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Nucleic Acid Denaturation (0):
Recent article
Nucleic Acid Denaturation
ISME Journal (4)1 2010
Abstract
We used molecular analytical methods to study the community structure of the gut microbiota in irradiated male medflies. We find that the sterilizing irradiation procedure affects the gut bacterial community structure of the Mediterranean fruit fly. Although the Enterobacteriaceae family remains the...
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PMID: 19617877
PDF is available here.
PDF is available here.
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Abstract
A series of novel water-soluble beta-carbolines bearing a flexible amino side chain was designed, synthesized and evaluated as potent cytotoxic and DNA intercatalating agents. The N(9)-arylated alkyl substituted beta-carbolines represented the most interesting cytotoxic activities. The results sugge...
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PMID: 20606334
PDF is available here.
PDF is available here.
Abstract
We find that removal of the anionic C-terminal domain (CTD) of HTLV-1 NC results in a protein with chaperone properties comparable with that of other retroviral NCs. Increasing the ionic strength of the solution also improves the chaperone activity of full-length HTLV-1 NC. To determine how the CTD...
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PMID: 19887455
PDF is available here.
PDF is available here.
Abstract
We performed DHPLC and DNA sequencing to study exons 9, 11, 13, and 17 of c-KIT and exons 12 and 18 of PDGFRA in 54 consecutive cases of GIST collected from a single population. Most (40/54 [74%]) carried c-KIT mutations, and 7 (13%) carried PDGFRA mutations. These results were similar to those desc...
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PMID: 20023271
PDF is available here.
PDF is available here.
Abstract
Gastrointestinal stromal tumors (GISTs) are mesenchymal tumors characterized by mutations of KIT or PDGFRA. The objectives of this study were to evaluate BRAF mutations in GISTs and then to correlate BRAF mutational status in the tumor with clinical parameters, with B-raf expression,...
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PMID: 20023270
PDF is available here.
PDF is available here.
Physical Review Letters (103)24 2009
Abstract
We unzip DNA molecules using optical tweezers and determine the sizes of the cooperatively unzipping and zipping regions separating consecutive metastable intermediates along the unzipping pathway. Sizes are found to be distributed following a power law, ranging from one base pair up to more than a...
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PMID: 20366235
PDF is available here.
PDF is available here.
Abstract
We sought to streamline semiconductor nanocrystal synthesis and develop synthetic routes that would be accessible to scientists from all disciplines. In search of such an approach, we turned to nucleic acids as a programmable and versatile ligand set and found that these biomolecules are indeed appr...
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PMID: 19947595
PDF is available here.
PDF is available here.
Analytical Biochemistry (398)1 2009
Abstract
We report the utilization of HRM for genotyping of the 1kb insertion/deletion polymorphism, involving a problematic region of five consecutive Alu elements, which is associated with myotonic dystrophy type 1. We combined a three-primer PCR amplification approach with HRM using two primer sets. Analy...
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PMID: 19903450
PDF is available here.
PDF is available here.
Georgian medical news ()175 2009
Abstract
The influence of a new anticarcinogenic compound Cu(II)TOEPyP(4) (Cu(II)-Meso-tetra(4-N-oxyethylpyridyl) porphyrin) (the analogue of TMPy4[tetra(N-mrthyl-4-pyridyl)-porphyrin chloride] - an inhibitor of telomerase activity - on the thermostability of calf thymus DNA in vitro has been studied. It has...
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PMID: 19893128
PDF is available here.
PDF is available here.
Abstract
We report the three-dimensional structure of a DNA duplex with a 3'-terminal 2'-anthraquinoylamido-2'-deoxyuridine (Uaq) residue that was solved by NMR and restrained molecular dynamics. The Uaq residue binds the 5'-terminus of the target strand through a combination of pi-stacking, hydrogen bonding...
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PMID: 19722718
PDF is available here.
PDF is available here.
Abstract
We have examined the biological activity of 12 platinum(II)-based DNA intercalators of the type [Pt(I(L))(A(L))](2+), where I(L) is an intercalating ligand (1,10-phenanthroline or a methylated derivative) and A(L) is an ancillary ligand (diaminocyclohexane, diphenylethylenediamine or 1,2-bis(4-fluor...
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PMID: 19658404
PDF is available here.
PDF is available here.
Journal of Neuroscience Research (65)Pt 9 2009
Abstract
Locked nucleic acids (LNAs) are modified nucleic acids which contain a modified sugar such as beta-D-2'-O,4'-C methylene-bridged ribofuranose or other sugar derivatives in LNA analogues. The beta-D-2'-O,4'-C methylene ribofuranose LNAs in particular possess high stability and melting temperatures, wh...
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PMID: 19724123
PDF is available here.
PDF is available here.
Abstract
Microbial populations are primarily responsible for the decomposition of organic residues, the nutrients cycle, and the flow of energy inside of soil. The present study was undertaken to link soil microbiological and soil biochemical parameters with soil- and litter-quality conditions in the surface...
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PMID: 19898551
PDF is available here.
PDF is available here.
Abstract
We examined the methanotrophs in the Trail Road Landfill soils, Ottawa, Ontario, through cultivation-independent molecular assay and the culturing approach. Denaturing gradient gel electrophoresis (DGGE) analysis of amplified methanotroph-specific 16S rDNA gene fragments revealed a more diverse type...
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PMID: 19898553
PDF is available here.
PDF is available here.
Abstract
We report the DNA interactions and activity in living cells of six monosubstituted dirhodium(II,II) complexes of general formula [Rh(2)(mu-O(2)CCH(3))(2)(eta(1)-O(2)CCH(3))(L)(CH(3)OH)](+), where L = bpy (2,2'-bipyridine) (1), phen (1,10-phenanthroline) (2), dpq (dipyrido[3,2-f:2',3'-h]quinoxaline)...
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PMID: 19624128
PDF is available here.
PDF is available here.
Abstract
Study of interaction between DNA and intercalator at molecular level is important to understand the mechanisms of DNA replication and repair. A micro-fabricated local heating thermodevice was adapted to perform denaturation experiments of DNA with fluorescent intercalator on millisecond time scale....
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PMID: 19576472
PDF is available here.
PDF is available here.
Radiation Research (172)2 2009
Abstract
We re-examine here DSB repair in several D-NHEJ-deficient mutants using low-temperature lysis. The results demonstrate extensive but slow repair of DSBs after low-temperature lysis indicative of a robust function of B-NHEJ. At the same time, the results demonstrate a pronounced sensitivity of heat-l...
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PMID: 19630520
PDF is available here.
PDF is available here.
Abstract
We have designed a TFO with a platinum-modified base that can interact with and cross-link to a cytosine interruption in the polypurine tract of a target DNA duplex. The TFO contains an N(4)-(aminoalkyl)cytosine derivatized with cis-diamminediaquaplatinum(II) or trans-diamminediaquaplatinum(II). Whe...
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PMID: 19350290
PDF is available here.
PDF is available here.
Abstract
In the context of the design and synthesis of DNA ligands, some new hetarene annelated carbazoles were synthesized. As lead structure the intercalating tetracyclic systems pyrido[2,3-a]- and pyrido[4,3-a]-carbazoles and in one case a thieno[2,3-a]-carbazole were taken into account. A...
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PMID: 19386396
PDF is available here.
PDF is available here.
Abstract
Our study demonstrates that the use of different primer pairs generate significant differences in DGGE analysis of enterococcal population, consequently, appropriate primers regarding the purpose of analysis can be selected. For differentiation and identification of pure enterococcal isolates, prime...
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PMID: 19250575
PDF is available here.
PDF is available here.
Biophysical Journal (97)2 2009
Abstract
We have made these measurements using calorimetric and ultraviolet hypochromicity methods, as well as denaturing gradient gel electrophoretic methods. The results suggest that in relaxed conditions, a system that meets the pairing requirements for PX-DNA would prefer to form the PX motif relative to...
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PMID: 19619467
PDF is available here.
PDF is available here.
Abstract
Patients with acute myeloid leukemia treated with chemotherapy and prophylactic antibiotics are unable to maintain colonization resistance because of a decrease in anaerobic bacteria and an increase in potentially pathogenic aerobic enterococci. We hypothesize that this disturbance in the balance be...
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PMID: 19514856
PDF is available here.
PDF is available here.
Abstract
Combined heat and chemical denaturation of double-stranded DNA scaffold strands in the presence of staple strands, followed by a sudden temperature drop and then stepwise dialysis to remove the chemical denaturant, leads to self-assembly of two distinct DNA-origami structures.
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PMID: 19566089
PDF is available here.
PDF is available here.
Audio, Transactions of the IRE Professional Group on (Chapter 18) 2009
Abstract
We describe a set of improvements we have made to the standard Illumina Genome Analyzer protocols to make the sequencing process more reliable in a high-throughput environment, reduce amplification bias, narrow the distribution of insert sizes, and reliably obtain high yields of data....
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PMID: 19582764
PDF is available here.
PDF is available here.
Abstract
A series of amide-coupled bisanthrapyrazole derivatives of 7-chloro-2-[2-[(2-hydroxyethyl)methylamino]ethyl]anthra[1,9-cd]pyrazol-6(2H)-one (AP9) were designed using molecular modeling and docking and synthesized in order to develop an anticancer drug that formed a strongly binding b...
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PMID: 19457675
PDF is available here.
PDF is available here.