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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Nucleic Acid Hybridization (5):
Recent article
Nucleic Acid Hybridization
Abstract
We report SNP and CNV haplotype structures determined by analysis of 100 CHMs from Japanese subjects via high-density DNA arrays. The obtained haplotype map should be useful as a reference for the haplotype structure of Asian populations. We resolved common CNV regions (merged CNV segments across th...
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PMID: 20537301
PDF is available here.
PDF is available here.
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Analytical Biochemistry (401)2 2010
Abstract
The preparation of DNA-tagged liposomes containing an encapsulated prosthetic group tracer, pyrroloquinoline quinone (PQQ), and their application to the development of a sandwich-type hybridization assay for the visual detection of single-stranded DNA are described. Capture DNA is conjugated to the...
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PMID: 20206593
PDF is available here.
PDF is available here.
Abstract
Mitochondrial respiratory chain (RC) disorders (RCDs) are a group of genetically and clinically heterogeneous diseases because of the fact that protein components of the RC are encoded by both mitochondrial and nuclear genomes and are essential in all cells. In addition, the biogenesis, structure, a...
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PMID: 20818730
PDF is available here.
PDF is available here.
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (18)6 2010
Abstract
To establish a method for simultaneous detection of HBV resistant mutations associated with three kinds of nucleoside analogues.
According to 981 HBV complete sequences in GenBank, two pairs of conserved primers labeled with digoxigenin were synthesized to amplify the region of HBV reverse transcrip...
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PMID: 20587309
PDF is available here.
PDF is available here.
Abstract
It has been proposed that regions of microhomology in the human genome could facilitate genomic rearrangements, copy number transitions, and rapid genomic change during tumor progression. To investigate this idea, this study examines the role of repetitive sequence elements, and corresponding synten...
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PMID: 20453501
PDF is available here.
PDF is available here.
Abstract
DNA clones containing highly repetitive DNA sequences were selected from partial libraries of Lathyrus sativus and L. sylvestris. Two satellite DNA sequence families were isolated from the genome of the former species. A first family was made up of repeats that varied in length from 54-56 bp, and sh...
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PMID: 20424423
PDF is available here.
PDF is available here.
Antonie Van Leeuwenhoek (98)1 2010
Abstract
During the course of our research on new actinobacterial sources, a novel actinomycete strain YIM 67013(T) was isolated from rhizosphere soil of Tripterygium wilfordii Hook. f. collected from Yunnan province, south-west China. The strain formed well-differentiated aerial and substrate mycelia, grew...
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PMID: 20354787
PDF is available here.
PDF is available here.
Abstract
We screened 85 isolates of Bacillus thuringiensis (Berliner), making up 57 different subspecies, and two isolates of Bacillus sphaericus (Meyer and Neide) for activity against immature horn flies, Haematobia irritans (L.), and stable flies, Stomoxys calcitrans (L.). The majority of B. thuringiensis...
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PMID: 20568651
PDF is available here.
PDF is available here.
Nucleic Acids Research (38)11 2010
Abstract
We have recently described a Dilute-'N'-Go protocol that greatly simplifies preparation and sequencing of both strands of an amplicon generated using linear-after-the-exponential (LATE)-PCR, an advanced form of asymmetric PCR . The same protocol can also be used to sequence all limiting primer stran...
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PMID: 20189962
PDF is available here.
PDF is available here.
Nucleic Acids Research (38)11 2010
Abstract
We could successfully photoregulate their hybridization with mRNA and target RNA hydrolysis by RNase H with light activation....
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PMID: 20164090
PDF is available here.
PDF is available here.
Abstract
A sensitive and selective ligase-based signal-on electrochemical sensing method for adenosine-5'-triphosphate (ATP) detection had been developed using molecular beacon (MB)-like DNA. In this method, the biotin-tagged MB-like DNA was self-assembled onto a gold electrode to form a stem-loop structure...
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PMID: 20299199
PDF is available here.
PDF is available here.
Abstract
We detected the hybridization of HPV PCR products during temperature change process by a self-designed adjustable metal-clamping piezoelectric sensor. The study mainly involves sensor adjustment, probe immobilization and ice bath sample addition (at different concentrations and different volumes). T...
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PMID: 20303735
PDF is available here.
PDF is available here.
Abstract
An ultrasensitive assay for electrical biosensing of syphilis DNA was proposed by using target-guided formation of polyaniline (PANI) based on an enzymatically catalyzed method. The sensitive biodetection relies on the DNA hybridization and biotin-streptavidin interaction. After coupling of biotinyl...
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PMID: 20207129
PDF is available here.
PDF is available here.
Abstract
We report on a rapid method for DNA hybridisation and isolation within a microfluidic device, where all reaction and washing steps are performed in continuous flow in an automated fashion within less than two minutes. Magnetic particles were used as a solid support and manipulated through laminar fl...
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PMID: 20181469
PDF is available here.
PDF is available here.
Abstract
Target recognizing ternary mixed monolayer is constructed by simple sequential adsorption of thiol caped single stranded DNA (HS-ssDNA), 6-mercapto-1-hexanol (MCH) and 3-mercaptopropionic acid (MPA) diluents (abbreviated as HS-ssDNA/MPA/MCH) and applied for detection of DNA hybridization sequence by...
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PMID: 20303736
PDF is available here.
PDF is available here.
Chemical Communications (46)18 2010
Abstract
A signal amplification based on a combination of hybridization chain reaction (HCR) and enzyme-enhancement was applied in the study of a novel biosensor. The target sequence was added as catalyst which was simply transmitted but did not disappear as the DNA double strand grew on the surface of magne...
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PMID: 20424746
PDF is available here.
PDF is available here.
Abstract
We employed DNA-functionalized gold nanoparticles (AuNPs) as a model system and found that if the alcohol content is less than approximately 30%, more alcohol leads to a faster DNA hybridization, although with a decreased melting temperature. The generality of this observation was independently veri...
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PMID: 20402504
PDF is available here.
PDF is available here.
Abstract
A method for the electrical contacting of redox enzymes that obtain oxidizing or reducing equivalents from small electron-transfer proteins is demonstrated. The electrochemical contacting of redox enzymes through their immobilization onto electrode supports offers great potential for technological a...
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PMID: 20397667
PDF is available here.
PDF is available here.
Abstract
The main objective of the present work was to develop a wheat genotype containing both the recessive crossability alleles (kr1kr1kr2kr2), allowing high crossability between 6x wheat and diploid rye, and the 1BL.1RS wheat/rye translocation chromosome. This wheat genotype could be used as a recipient...
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PMID: 20145905
PDF is available here.
PDF is available here.
Abstract
The objective of the present paper is to develop and apply a multiplex polymerase chain reaction (mPCR) based reverse line blot (RLB) hybridization assay to facilitate the diagnosis of genital infections by detection of seven recognized or putative genital pathogens (Neisseria gonorrhoeae, Chlamydia...
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PMID: 20498099
PDF is available here.
PDF is available here.
Abstract
We summarize the rules to predict miRNA targets and introduce some computational algorithms that have been developed for miRNA target prediction and the application of the methods. In addition, the issue of target gene validation in an experimental way will be discussed....
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PMID: 20177143
PDF is available here.
PDF is available here.
Abstract
A new label-free, optical method was developed to detect sequence-specific DNA based on supramolecular self-assembly. A cationic phenylene ethynylene oligomer with two pairs of positively charged side chains (OPE-2) can form a J-dimer or J-aggregate with negatively charged DNA by a combination of el...
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PMID: 20030336
PDF is available here.
PDF is available here.
Abstract
We present an ultrasensitive electrochemical DNA sensor using (i) single DNA hybridization with peptide nucleic acid (PNA), (ii) selective binding of [Ru(NH(3))(6)](3+) to hybridized DNA, (iii) fast NaBH(4) electrooxidation mediated by [Ru(NH(3))(6)](3+), and (iv) low background currents of NaBH(4)...
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PMID: 20085331
PDF is available here.
PDF is available here.
Abstract
We present a new method to prepare inverse opaline photonic beads with good spherical shape and superior optical performance by simply introducing an interfacial tension system into a template replication method. When the scaffolds of these beads were composed of poly(ethylene glycol) diacrylate hyd...
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PMID: 20359181
PDF is available here.
PDF is available here.
Abstract
Persistent West Nile virus (WNV) infection in the mosquito Culex quinquefasciatus Say (Diptera: Culicidae) is associated with pathological changes in the salivary glands, including apoptotic cell death and a corresponding reduction in virus transmission over time. The vector host response to WNV inf...
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PMID: 20496590
PDF is available here.
PDF is available here.