Abstract
We summarized recent research progresses on avirulence genes, which are the most important effectors in M. grisea with the focus on chromosome mapping, cloning method, functional analysis, and evolution study of avirulence genes, and the possible hotspot of the research on avirulence genes in the fu...
|
PMID: 21684864
PDF is available here.
Abstract
The analysis of transgene inheritance is an important step in the molecular and genetic characterization of transgenes. In this manuscript, two approaches to characterize the inheritance of transgenes are described. The first approach is based on the expression of the transgene phenotype and the sec...
|
PMID: 19378007
PDF is available here.
Abstract
Backcross breeding enables breeders to transfer a desired trait such as a transgene from one variety (donor parent, DP) into the favored genetic background of another (recurrent parent, RP). If the trait of interest is produced by a dominant gene, this process involves four rounds of backcrossing wi...
|
PMID: 19378006
PDF is available here.
Abstract
Detection of osteogenic differentiation is crucial for bone tissue engineering. Despite established standard end point assays, there is increasing demand for methods allowing noninvasive kinetic differentiation monitoring. Reporter gene assays employing tissue-specific promoters and...
|
PMID: 21043997
PDF is available here.
David S P DS Tan,
Marjan M Iravani,
W Glenn WG McCluggage,
Maryou B K MB Lambros,
Fernanda F Milanezi,
Alan A Mackay,
Charles C Gourley,
Felipe C FC Geyer,
Radost R Vatcheva,
Joanne J Millar,
Karen K Thomas,
Rachael R Natrajan,
Kay K Savage,
Kerry K Fenwick,
Alistair A Williams,
Charles C Jameson,
Mona M El-Bahrawy,
Martin E ME Gore,
Hani H Gabra,
Stanley B SB Kaye,
Alan A Ashworth and
Jorge S JS Reis-Filho
Abstract
Ovarian clear cell carcinomas (OCCC) are a drug-resistant and aggressive type of epithelial ovarian cancer. We analyzed the molecular genetic profiles of OCCCs to determine whether distinct genomic subgroups of OCCCs exist.
|
PMID: 21411445
PDF is available here.
Abstract
For nearly 100 years, developmental biologists have utilized fate mapping to understand the contributions of progenitor populations to organogenesis. More recently, Cre-Lox technology has allowed genetic fate mapping in adult mice, clarifying cell hierarchies in adult kidney disease...
|
PMID: 20861816
PDF is available here.
Abstract
For nearly 100 years, developmental biologists have utilized fate mapping to understand the contributions of progenitor populations to organogenesis. More recently, Cre-Lox technology has allowed genetic fate mapping in adult mice, clarifying cell hierarchies in adult kidney disease...
|
PMID: 20861816
PDF is available here.
Abstract
We intended to explore a strategy to quickly identify somatic mutations in the entire mtDNA genome based on its phylogeny. The principal assumption for this strategy is that somatic mutations, as recently accumulated in cancerous tissue, have younger age and will be located in the terminal branches...
|
PMID: 21419139
PDF is available here.
Abstract
A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1-H13, H15, H16) and neuraminidase (N1-N9). Additional sets of probes are used to detect H1N1 sw...
|
PMID: 21559081
PDF is available here.
Abstract
Reaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonu...
|
PMID: 21225639
PDF is available here.
Abstract
We here describe RNA extraction from one or a few frozen tissue sections and subsequent analysis of structural RNA integrity by microcapillary gel electrophoresis....
|
PMID: 20949406
PDF is available here.
Abstract
We have used RNA interference to selectively suppress the expression of aggrecanase genes in human chondrocytes, in an attempt to determine which of these key enzymes have roles in arthritic cartilage destruction. This combination of gene targeting and tissue engineering we are using should be equal...
|
PMID: 21042968
PDF is available here.
Abstract
Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells can self-renew while maintaining their pluripotency to differentiate into virtually all cell types. In addition to their potential for regenerative medicine, hESCs and iPSCs can also serve a...
|
PMID: 21042988
PDF is available here.
Abstract
We review the protocols for a yeast-based assay to detect effective ZFNs. Additionally, we detail the procedures for synthesis and injection of ZFN-encoding mRNA into zebrafish embryos, screening of injected embryos for induced mutations in the soma, and recovery of germline mutations....
|
PMID: 21805278
PDF is available here.
Wulf Haubensak,
Prabhat S Kunwar,
Haijiang Cai,
Stephane Ciocchi,
Nicholas R Wall,
Ravikumar Ponnusamy,
Jonathan Biag,
Hong-Wei Dong,
Karl Deisseroth,
Edward M Callaway,
Michael S Fanselow,
Andreas Lüthi and
David J Anderson
Abstract
We use molecular genetic approaches to map the functional connectivity of a subpopulation of GABA-containing neurons, located in the lateral subdivision of the central amygdala (CEl), which express protein kinase C-δ (PKC-δ). Channelrhodopsin-2-assisted circuit mapping in amygdala slices and cell-...
|
PMID: 21068836
PDF is available here.
Abstract
Numerous molecular abnormalities have been described in lymphomas. They are of diagnostic and prognostic value and are taken into account for the WHO classification of these tumors. They also shed some light on the underlying molecular mechanisms involved in lymphomas. Overall, four types of molecul...
|
PMID: 21084243
PDF is available here.
Anthony A Clemons,
Morgan M Haugen,
Ellen E Flannery,
Michael M Tomchaney,
Kristopher K Kast,
Caitlin C Jacowski,
Christy C Le,
Akio A Mori,
Wendy W Simanton Holland,
Joseph J Sarro,
David W DW Severson and
Molly M Duman-Scheel
Abstract
Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimul...
|
PMID: 20889691
PDF is available here.
Abstract
Fig, Ficus carica L., is a useful genetic resource for commercial cultivation. In this study, RAPD (60), ISSR (48), RAMPO (63), and SSR (34) markers were compared to detect polymorphism and to establish genetic relationships among Tunisian fig tree cultivars. The statistical procedures conducted on...
|
PMID: 20628809
PDF is available here.
Abstract
Cells devote a significant amount of metabolism to maintaining the stability of their genome and to preventing inappropriate chromosomal rearrangements that are characteristic of many cancers. A simple genetic assay using haploid derivatives of the yeast Saccharomyces cerevisiae provides a means to...
|
PMID: 20810639
PDF is available here.
Abstract
The recent development of molecular biology methods has substantially improved the identification of many bacterial pathogens, both at the species and strain level. Microbial strain genotyping refers to the process of discriminating among individuals within particular species based on the detection...
|
PMID: 20931834
PDF is available here.
Abstract
At present, molecular typing methods of Mycobacterium tuberculosis have become increasingly integrated into the epidemiological studies of tuberculosis. Molecular typing is a process of discriminating between strains of tubercle bacilli, based on the detection of genomic DNA polymorphisms, most freq...
|
PMID: 20931835
PDF is available here.
Masatoshi Yamaguchi,
Nadia Goué,
Hisako Igarashi,
Misato Ohtani,
Yoshimi Nakano,
Jennifer C Mortimer,
Nobuyuki Nishikubo,
Minoru Kubo,
Yoshihiro Katayama,
Koichi Kakegawa,
Paul Dupree and
Taku Demura
Abstract
We previously showed that the VASCULAR-RELATED NAC-DOMAIN6 (VND6) and VND7 genes, which encode NAM/ATAF/CUC domain protein transcription factors, act as key regulators of xylem vessel differentiation. Here, we report a glucocorticoid-mediated posttranslational induction system of VND6 and VND7. In t...
|
PMID: 20488898
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific...
|
PMID: 20498459
PDF is available here.