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Genetic Techniques (28):
  • Nucleic Acid Amplification Techniques
  • Cytogenetic Analysis
  • Sequence Analysis
  • Sequence Alignment
  • Nuclease Protection Assays
  • Gene Expression Profiling
  • DNA Footprinting
  • Contig Mapping
  • Heterozygote Detection
  • Chromatin Immunoprecipitation
  • Restriction Mapping
  • In Situ Nick-End Labeling
  • Crosses, Genetic
  • Epidemiology, Molecular
  • Molecular Probe Techniques
  • Gene Transfer Techniques
  • Gene Targeting
  • Molecular Diagnostic Techniques
  • DNA Fingerprinting
  • Pedigree
  • Nucleic Acid Hybridization
  • Nuclear Transfer Techniques
  • Genetic Engineering
  • Cloning, Organism
  • Nucleic Acid Denaturation
  • Cloning, Molecular
  • Chromosome Mapping
  • Mutagenicity Tests
Home > Investigative Techniques > Genetic Techniques

Recent article

Genetic Techniques

Fungal Biology (115)8 2011
High-resolution melting analysis: a new molecular approach for the early detection of Diplodia pinea in Austrian pine.
Nicola Luchi, Nicola Pratesi, Lisa Simi, Mario Pazzagli, Paolo Capretti, Aniello Scala, Bernard Slippers and Pamela Pinzani
Abstract
We applied one of the most sensitive and rapid diagnostic screening method so far developed, called High-Resolution Melting Analysis (HRMA), to detect D. pinea in Austrian pine (Pinus nigra). HRMA exploits differences in the melting behaviour of PCR products to rapidly identify DNA sequence variants... | PMID: 21802051

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RNA (33)6 2011
[Progress on avirulence genes of the rice blast fungus Magnaporthe grisea].
Zhe Z Zhang, Hua H Jiang, Yan-Li YL Wang and Guo-Chang GC Sun
Abstract
We summarized recent research progresses on avirulence genes, which are the most important effectors in M. grisea with the focus on chromosome mapping, cloning method, functional analysis, and evolution study of avirulence genes, and the possible hotspot of the research on avirulence genes in the fu... | PMID: 21684864

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Xenobiotica (41)5 2011
Case report of extensive metabolism by aldehyde oxidase in humans: Pharmacokinetics and metabolite profile of FK3453 in rats, dogs, and humans
Takafumi Akabane, Kohichiro Tanaka, Megumi Irie, Shigeyuki Terashita and Toshio Teramura
Abstract
The analysis of transgene inheritance is an important step in the molecular and genetic characterization of transgenes. In this manuscript, two approaches to characterize the inheritance of transgenes are described. The first approach is based on the expression of the transgene phenotype and the sec... | PMID: 19378007

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Xenobiotica (41)5 2011
Case report of extensive metabolism by aldehyde oxidase in humans: Pharmacokinetics and metabolite profile of FK3453 in rats, dogs, and humans
Takafumi Akabane, Kohichiro Tanaka, Megumi Irie, Shigeyuki Terashita and Toshio Teramura
Abstract
Backcross breeding enables breeders to transfer a desired trait such as a transgene from one variety (donor parent, DP) into the favored genetic background of another (recurrent parent, RP). If the trait of interest is produced by a dominant gene, this process involves four rounds of backcrossing wi... | PMID: 19378006

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Tissue Engineering, Part C: Methods (17)4 2011
Enhanced reporter gene assay for the detection of osteogenic differentiation.
Georg A Feichtinger, Tatjana J Morton, Alice Zimmermann, Daniela Dopler, Asmita Banerjee, Heinz Redl and Martijn van Griensven
Abstract
Detection of osteogenic differentiation is crucial for bone tissue engineering. Despite established standard end point assays, there is increasing demand for methods allowing noninvasive kinetic differentiation monitoring. Reporter gene assays employing tissue-specific promoters and... | PMID: 21043997

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Clinical Cancer Research (17)6 2011
Genomic analysis reveals the molecular heterogeneity of ovarian clear cell carcinomas.
David S P DS Tan, Marjan M Iravani, W Glenn WG McCluggage, Maryou B K MB Lambros, Fernanda F Milanezi, Alan A Mackay, Charles C Gourley, Felipe C FC Geyer, Radost R Vatcheva, Joanne J Millar, Karen K Thomas, Rachael R Natrajan, Kay K Savage, Kerry K Fenwick, Alistair A Williams, Charles C Jameson, Mona M El-Bahrawy, Martin E ME Gore, Hani H Gabra, Stanley B SB Kaye, Alan A Ashworth and Jorge S JS Reis-Filho
Abstract
Ovarian clear cell carcinomas (OCCC) are a drug-resistant and aggressive type of epithelial ovarian cancer. We analyzed the molecular genetic profiles of OCCCs to determine whether distinct genomic subgroups of OCCCs exist. | PMID: 21411445

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Kidney International (79)5 2011
Origin of new cells in the adult kidney: results from genetic labeling techniques.
Jeremy S Duffield and Benjamin D Humphreys
Abstract
For nearly 100 years, developmental biologists have utilized fate mapping to understand the contributions of progenitor populations to organogenesis. More recently, Cre-Lox technology has allowed genetic fate mapping in adult mice, clarifying cell hierarchies in adult kidney disease... | PMID: 20861816

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Kidney International (79)5 2011
Origin of new cells in the adult kidney: results from genetic labeling techniques.
Jeremy S Duffield and Benjamin D Humphreys
Abstract
For nearly 100 years, developmental biologists have utilized fate mapping to understand the contributions of progenitor populations to organogenesis. More recently, Cre-Lox technology has allowed genetic fate mapping in adult mice, clarifying cell hierarchies in adult kidney disease... | PMID: 20861816

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Mutation Research (709-710) 2011
Rapid identification of mtDNA somatic mutations in gastric cancer tissues based on the mtDNA phylogeny.
Rui R Bi, Wen-Liang WL Li, Ming-Qing MQ Chen, Zhu Z Zhu and Yong-Gang YG Yao
Abstract
We intended to explore a strategy to quickly identify somatic mutations in the entire mtDNA genome based on its phylogeny. The principal assumption for this strategy is that somatic mutations, as recently accumulated in cancerous tissue, have younger age and will be located in the terminal branches... | PMID: 21419139

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PLoS ONE (6)4 2011
Universal oligonucleotide microarray for sub-typing of Influenza A virus.
Vladimir A Ryabinin, Elena V Kostina, Galiya A Maksakova, Alexander A Neverov, Konstantin M Chumakov and Alexander N Sinyakov
Abstract
A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1-H13, H15, H16) and neuraminidase (N1-N9). Additional sets of probes are used to detect H1N1 sw... | PMID: 21559081

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Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.] (Chapter 3) 2011
Endonucleases.
Nicole M Nichols
Abstract
Reaction conditions for a variety of endonucleases are detailed in this unit along with discussions of potential applications. Enzymes covered include BAL 31 nuclease, S1 nuclease, mung bean nuclease, micrococcal nuclease, and DNase I. A general discussion regarding the use of endonu... | PMID: 21225639

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Methods in Molecular Biology (675) 2011
Fresh frozen tissue: RNA extraction and quality control.
Johan Botling and Patrick Micke
Abstract
We here describe RNA extraction from one or a few frozen tissue sections and subsequent analysis of structural RNA integrity by microcapillary gel electrophoresis.... | PMID: 20949406

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Methods in Molecular Biology (695) 2011
Genetic modification of chondrocytes using viral vectors.
Teresa Coughlan, Aileen Crawford, Paul Hatton and Michael Barker
Abstract
We have used RNA interference to selectively suppress the expression of aggrecanase genes in human chondrocytes, in an attempt to determine which of these key enzymes have roles in arthritic cartilage destruction. This combination of gene targeting and tissue engineering we are using should be equal... | PMID: 21042968

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Methods in Molecular Biology (690) 2011
Manipulations of microRNA in human pluripotent stem cells and their derivatives.
Stephanie N Rushing, Anthony W Herren, Deborah K Lieu and Ronald A Li
Abstract
Human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) reprogrammed from somatic cells can self-renew while maintaining their pluripotency to differentiate into virtually all cell types. In addition to their potential for regenerative medicine, hESCs and iPSCs can also serve a... | PMID: 21042988

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Methods in Molecular Biology (770) 2011
Inducing high rates of targeted mutagenesis in zebrafish using zinc finger nucleases (ZFNs).
Jasmine M McCammon, Yannick Doyon and Sharon L Amacher
Abstract
We review the protocols for a yeast-based assay to detect effective ZFNs. Additionally, we detail the procedures for synthesis and injection of ZFN-encoding mRNA into zebrafish embryos, screening of injected embryos for induced mutations in the soma, and recovery of germline mutations.... | PMID: 21805278

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Nature (468)7321 2010
Genetic dissection of an amygdala microcircuit that gates conditioned fear.
Wulf Haubensak, Prabhat S Kunwar, Haijiang Cai, Stephane Ciocchi, Nicholas R Wall, Ravikumar Ponnusamy, Jonathan Biag, Hong-Wei Dong, Karl Deisseroth, Edward M Callaway, Michael S Fanselow, Andreas Lüthi and David J Anderson
Abstract
We use molecular genetic approaches to map the functional connectivity of a subpopulation of GABA-containing neurons, located in the lateral subdivision of the central amygdala (CEl), which express protein kinase C-δ (PKC-δ). Channelrhodopsin-2-assisted circuit mapping in amygdala slices and cell-... | PMID: 21068836

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Bulletin du cancer (97)11 2010
[Molecular abnormalities in lymphomas].
G Delsol
Abstract
Numerous molecular abnormalities have been described in lymphomas. They are of diagnostic and prognostic value and are taken into account for the WHO classification of these tumors. They also shed some light on the underlying molecular mechanisms involved in lymphomas. Overall, four types of molecul... | PMID: 21084243

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Audio, Transactions of the IRE Professional Group on (2010)10 2010
Aedes aegypti: an emerging model for vector mosquito development.
Anthony A Clemons, Morgan M Haugen, Ellen E Flannery, Michael M Tomchaney, Kristopher K Kast, Caitlin C Jacowski, Christy C Le, Akio A Mori, Wendy W Simanton Holland, Joseph J Sarro, David W DW Severson and Molly M Duman-Scheel
Abstract
Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimul... | PMID: 20889691

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Biochemical Genetics (48)9-10 2010
Development of molecular tools for characterization and genetic diversity analysis in Tunisian fig (Ficus carica) cultivars.
Khaled Chatti, Ghada Baraket, Ahmed Ben Abdelkrim, Olfa Saddoud, Messaoud Mars, Mokhtar Trifi and Amel Salhi Hannachi
Abstract
Fig, Ficus carica L., is a useful genetic resource for commercial cultivation. In this study, RAPD (60), ISSR (48), RAMPO (63), and SSR (34) markers were compared to detect polymorphism and to establish genetic relationships among Tunisian fig tree cultivars. The statistical procedures conducted on... | PMID: 20628809

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Audio, Transactions of the IRE Professional Group on (2010)9 2010
Determination of gross chromosomal rearrangement rates.
Christopher D CD Putnam and Richard D RD Kolodner
Abstract
Cells devote a significant amount of metabolism to maintaining the stability of their genome and to preventing inappropriate chromosomal rearrangements that are characteristic of many cancers. A simple genetic assay using haploid derivatives of the yeast Saccharomyces cerevisiae provides a means to... | PMID: 20810639

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Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego (29)171 2010
[Genotyping of Mycobacterium tuberculosis. An overview of the major research techniques (Part I)].
Tomasz T Jagielski, Ewa E Augustynowicz-Kopeć and Zofia Z Zwolska
Abstract
The recent development of molecular biology methods has substantially improved the identification of many bacterial pathogens, both at the species and strain level. Microbial strain genotyping refers to the process of discriminating among individuals within particular species based on the detection... | PMID: 20931834

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Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego (29)171 2010
[Genotyping of Mycobacterlum tuberculosis. An overview of the major research techniques (Part II)].
Tomasz T Jagielski, Ewa E Augustynowicz-Kopeć and Zofia Z Zwolska
Abstract
At present, molecular typing methods of Mycobacterium tuberculosis have become increasingly integrated into the epidemiological studies of tuberculosis. Molecular typing is a process of discriminating between strains of tubercle bacilli, based on the detection of genomic DNA polymorphisms, most freq... | PMID: 20931835

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Asian Journal of Andrology (12)5 2010
Novel approaches for the molecular classification of prostate cancer.
Robert H Getzenberg
Abstract
PMID: 20711216

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Plant Physiology (153)3 2010
VASCULAR-RELATED NAC-DOMAIN6 and VASCULAR-RELATED NAC-DOMAIN7 effectively induce transdifferentiation into xylem vessel elements under control of an induction system.
Masatoshi Yamaguchi, Nadia Goué, Hisako Igarashi, Misato Ohtani, Yoshimi Nakano, Jennifer C Mortimer, Nobuyuki Nishikubo, Minoru Kubo, Yoshihiro Katayama, Koichi Kakegawa, Paul Dupree and Taku Demura
Abstract
We previously showed that the VASCULAR-RELATED NAC-DOMAIN6 (VND6) and VND7 genes, which encode NAM/ATAF/CUC domain protein transcription factors, act as key regulators of xylem vessel differentiation. Here, we report a glucocorticoid-mediated posttranslational induction system of VND6 and VND7. In t... | PMID: 20488898

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Seikagaku. The Journal of Japanese Biochemical Society (82)7 2010
[Development of molecules binding to mismatched base pairs: application to the detection of gene mutation and PCR monitoring].
Kazuhiko K Nakatani
Abstract
PMID: 20715580

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Science (329)5987 2010
Genetic technologies. Synthetic "life," ethics, national security, and public discourse.
Mildred K Cho and David A Relman
Abstract
PMID: 20595601

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RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.
RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

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RNA (16)7 2010
Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs.
Huafeng Fang, Yuefei Shen and John-Stephen Taylor
Abstract
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific... | PMID: 20498459

Large_pdficon_selected PDF is available here.