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Immunologic Techniques (4):
Recent article
Immunologic Techniques
Investigacion clinica (52)1 2011
Abstract
In recent years, better diagnostics for tuberculosis (TB) has received increasing attention, especially the diagnosis of tuberculous pleural effusion, which is difficult and at present the main tool in TPE diagnostic is pleural effusion smear and culture, but unfortunately, sensitivi...
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PMID: 21612137
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Abstract
We prepared a polymer in which the Cu(II) was embedded. The Cu(II)-embedded polymer was tested for its binding with protein. It was found that Cu(II)-embedded polymer displayed a strong binding with bovine serum albumin (BSA). At 80% of methanol, no BSA was released from Cu(II)-embedded polymer. The...
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PMID: 20602958
PDF is available here.
PDF is available here.
Audio, Transactions of the IRE Professional Group on (Chapter 16) 2010
Abstract
The first issue in selecting a system for antigen-presentation experiments is to define the appropriate type of antigen-presenting cell (APC) to study. For some experiments, crude preparations such as splenocytes or peripheral blood mononuclear cells (PBMCs) may suffice to provide APC function for s...
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PMID: 20143315
PDF is available here.
PDF is available here.
Audio, Transactions of the IRE Professional Group on (Chapter 16) 2010
Abstract
Antigen processing and presentation experiments can be done with a wide variety of antigen-presenting cells (APCs). Most experiments will use one of the "professional" APC types: dendritic cells (DCs), macrophages, and B lymphocytes. Other types of cells may be used for antigen presentation in some...
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PMID: 20143316
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
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PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Transfusion (50)2 2010
Abstract
Since the first issue of TRANSFUSION in 1961, there has been a tremendous expansion in not only the number of blood group antigens identified but also in our knowledge of their biochemical basis, function, and more recently, associated DNA changes. As certain techniques became availa...
|
PMID: 19906040
PDF is available here.
PDF is available here.
Abstract
We recently reported that degenerating motor neurons of superoxide dismutase mutant 1 (SOD1) rodents exhibit immunoreactivity to P2X(4) antibodies. Neurons with strong P2X(4)-like immunoreactivity (P2X(4)-LIR) do not show an apoptotic phenotype and are often associated with microglial cells that dis...
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PMID: 20084016
PDF is available here.
PDF is available here.
Biotechnology Progress (26)6 2010
Abstract
We describe and demonstrate a technique for detection of cell surface antigens, with potential use in tissue antigen research. Briefly, a small volume of wetted chromatographic beads (specifically, 100 μL of Nickel-nitriloacetic acid (NTA) agarose) was bound to small quantities (specifically, ∼0....
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PMID: 20878645
PDF is available here.
PDF is available here.
Molecular Vision (16) 2010
Abstract
Aberrant T-cell-mediated processes may underlie ADNIV, and therapeutics directed at T cells may better manage inflammation in these patients. Genes related to T-cell function are high priority screening candidates....
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PMID: 20596252
PDF is available here.
PDF is available here.
Molecular Vision (16) 2010
Abstract
The extent of the variability of gene expression in RPE cells at the protein level can be quantified by LSC. Relative changes in the MFI, the CV, and/or percentage of RPE cells double labeled for a second antigen quantify the changes observed. The analysis of these data also suggest whether the effe...
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PMID: 20606706
PDF is available here.
PDF is available here.
Abstract
Staphylococcal enterotoxin B (SEB) is an extracellular pyrotoxin produced by Staphylococcus aureus, a known etiologic agent of food poisoning in humans. Lateral flow immunochromatographic devices (LFDs) designed for the environmental detection of SEB were adapted for use in this study to detect SEB...
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PMID: 20480905
PDF is available here.
PDF is available here.
Medicina nei secoli (22)1-3 2010
Abstract
I (1531-1587). These results are of particular interest not only because they confirm the historical data, but also because they demonstrate that immunological tests can be applied to bone samples, opening new paths of research for the screening of large skeletal series....
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PMID: 21563476
PDF is available here.
PDF is available here.
Parasitology (93 ( Pt 3))03 2009
Abstract
Adult Wuchereria bancrofti were recovered from infected Presbytis cristatus monkeys and radio-isotope labelled extrinsically with 125I and in vitro with [35S]methionine. 125I labelling of the surface of adult W. bancrofti permitted a comparison between the major surface antigens of this species and...
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PMID: 3540817
PDF is available here.
PDF is available here.
Parasitology (93 ( Pt 3))03 2009
Abstract
We reported the isolation of several Escherichia coli clones expressing fragments of Taenia taeniaeformis antigens as beta-galactosidase fused proteins (Bowtell, Saint, Rickard & Mitchell, 1984). Here we describe the isolation of additional antigen-expressing clones from a larval cDNA library and th...
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PMID: 3540818
PDF is available here.
PDF is available here.
Parasitology (93 ( Pt 2))02 2009
Abstract
Excretions and secretions (E-S) were collected from a series of developmental stages of Litomosoides carinii maintained in vitro. Measurement of the protein content of E-S obtained from each stage indicates that the rate of production of E-S varies enormously during development of the worm. E-S was...
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PMID: 3537924
PDF is available here.
PDF is available here.
Abstract
We evaluated simultaneous detection of 11 ANA and clinical utility as ANA screening test. We collected sera from 317 connective tissue disease (CTD) patients, 166 healthy subjects, and 105 sera in which anti dsDNA antibody(RIA) is requested. Detection of 11 ANA were measured by BioPlex2200 ANA Scree...
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PMID: 19928490
PDF is available here.
PDF is available here.
Biomaterials (30)22 2009
Abstract
These results demonstrate that cell surface coating promotes the attachment of MSCs to endothelial cells, and provides a methodology for the delivery of stem cells to sites of inflammation....
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PMID: 19375791
PDF is available here.
PDF is available here.
Current protocols in neuroscience / editorial board, Jacqueline N. Crawley ... [et al.] (Chapter 5) 2009
Abstract
Much of modern biology and biochemistry relies on the availability of highly specific antibodies for use in such ubiquitous techniques as immunohistochemistry, ELISAs, immunoprecipitation, and immunoblotting. Thus, the generation of large quantities of specific antibodies directed against proteins o...
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PMID: 19575471
PDF is available here.
PDF is available here.
Parasitology (92 ( Pt 3))03 2009
Abstract
Sera from 40 onchocerciasis patients from the Yemen Arab Republic with either mild localized forms of onchocerciasis, intermediate or severe localized forms of the disease or generalized forms of infection were studied with respect to their IgG and IgM response against Onchocerca volvulus antigens....
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PMID: 3526262
PDF is available here.
PDF is available here.
Parasitology (94 ( Pt 3))03 2009
Abstract
Laboratory studies on a group of 20 patients from the Rio Negro Valley, Colombia selected for detailed study showed that 14 gave antibody reactions on immunoassay consistent with Trypanosoma cruzi or T. rangeli infections. Four were diagnosed as having T. rangeli infection, 4 had mixed infections an...
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PMID: 2441341
PDF is available here.
PDF is available here.
Journal of Parasitology (95)3 2009
Abstract
An immunochromatographic assay (Chagas Stat-Pak) was evaluated for the detection of Trypanosoma cruzi antibodies in 4 species of wildlife reservoirs. Antibodies to T. cruzi were detected in raccoons (Procyon lotor) (naturally and experimentally infected) and degus (Octodon degu) (experimentally-infe...
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PMID: 19016578
PDF is available here.
PDF is available here.
Abstract
Abstract
Peripheral blood is the primary source of lymphoid cells for investigation of the human immune system. Its use is facilitated by Ficoll-Hypaque density gradient centrifugation-a simple and rapid method of purifying peripheral blood mononuclear cells (PBMC) that takes advantage of the density differe...
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PMID: 19347849
PDF is available here.
PDF is available here.
Parasitology (96 ( Pt 2))02 2009
Abstract
We isolated DNA from microfilaria of 6 filarial species (Acanthocheilonema viteae, Brugia malayi, Brugia pahangi, Dirofilaria immitis, Litomosoides carinii and Setaria digitatum), digested the DNA with several restriction endonucleases, prepared Southern blots and probed with 32P-labelled DNA probes...
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PMID: 2897655
PDF is available here.
PDF is available here.
Parasitology (94 ( Pt 1))01 2009
Abstract
Four monoclonal antibodies, raised against macroschizonts of Theileria parva, were studied to characterize their antigen binding specificity. The indirect fluorescent antibody test showed that the antigen(s) recognized were present in the macroschizont stage of the parasite life-cycle but not in pir...
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PMID: 3103046
PDF is available here.
PDF is available here.
Parasitology (94 ( Pt 1))01 2009
Abstract
A panel of immunologically and biochemically defined monoclonal antibody probes has been used in conjunction with immunocytochemical techniques to localize target antigens in sections of different life-cycle stages of Trichinella spiralis. Monoclonals that immunoprecipitate surface components from a...
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PMID: 3547257
PDF is available here.
PDF is available here.
Abstract
Our results demonstrate that the immunoaffinity column with immobilized BBA-2187 scFv antibody is efficient for the cleanup of BPA-contaminated water samples from different sources....
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PMID: 19102649
PDF is available here.
PDF is available here.
BMC Immunology (10)1 2009
Abstract
B cell enrichment from tonsils was achieved using RosetteSep with no modification to the whole blood procedure; however, the degree of purity depended on the extent of red blood cell contamination of the starting tonsil cell suspension. Addition of a 50-fold excess of allogeneic human red blood cell...
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PMID: 19473530
PDF is available here.
PDF is available here.
Abstract
We describe a novel and simple approach to purify intact vacuoles formed by L. pneumophila within Dictyostelium discoideum by using magnetic immunoseparation with an antibody against SidC, followed by density gradient centrifugation. To monitor LCV purification by fluorescence microscopy, we used Di...
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PMID: 18980612
PDF is available here.
PDF is available here.
Abstract
We describe here an example of LDA experiment assessing antigen-specific T cell proliferation of microcultures in the presence or absence of adjuvant and illustrate how to estimate the frequencies of precursor T cells using an online tool that we made publicly available....
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PMID: 19048215
PDF is available here.
PDF is available here.
Abstract
Abstract
We present assays to measure killing mediated by phagocytosis, by NETs, by a combination of both, and by granular extract. As examples, we use Candida albicans for fungal and Shigella flexneri for bacterial pathogens....
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PMID: 19089391
PDF is available here.
PDF is available here.