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Indicator Dilution Techniques (3):
Recent article
Indicator Dilution Techniques
Abstract
Cardiac output (CO) measurement is becoming increasingly important in the field of pediatric intensive care medicine and pediatric anesthesia. In the past few decades, various new technologies have been developed for the measurement of CO. Some of these methods are applicable to pediatric patients a...
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PMID: 20583887
PDF is available here.
PDF is available here.
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Analytical Biochemistry (401)2 2010
Abstract
Interassay variation of antibody-based routine tests hampers comparability of measurement results for growth hormone (GH) between different laboratories and decision making in clinical practice. Here it is demonstrated that quantification of GH by isotope dilution mass spectrometry (IDMS) constitute...
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PMID: 20226156
PDF is available here.
PDF is available here.
British Journal of Nutrition (103)12 2010
Abstract
The objective was to quantify the vitamin A equivalency of beta-carotene in two diets using a dual-isotope dilution technique and the apparent beta-carotene absorption as measured by the oral-faecal balance technique. Seventeen healthy adults with an ileostomy completed the 4-week diet-controlled, c...
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PMID: 20132586
PDF is available here.
PDF is available here.
Abstract
We evaluated whether early VABF may predict long-term vascular access (VA) patency in incident HD patients. We enrolled 57 patients, of whom 27 were starting HD with arteriovenous fistulas (AVFs) and 30 with arteriovenous grafts (AVGs). The patients' VABF was measured monthly with the ultrasound dil...
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PMID: 20436709
PDF is available here.
PDF is available here.
Animal Reproduction Science (119)1-2 2010
Abstract
Despite considerable cryobiology research there is no industry standard for the concentration to which ram spermatozoa should be diluted before freezing. Ram semen is highly concentrated and often frozen at a high sperm concentration, necessitating the use of small laparoscopic insemination doses. T...
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PMID: 20060663
PDF is available here.
PDF is available here.
Abstract
This study assessed the spectrum of activity of torezolid (TR-700), the active moiety of torezolid phosphate (TR-701), and proposes tentative MIC and disk diffusion breakpoints as well as quality control ranges. The in vitro susceptibilities of 1,096 bacterial isolates, representing 23 different spe...
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PMID: 20231392
PDF is available here.
PDF is available here.
Abstract
In the setting of airflow obstruction, Pleth systematically overestimates lung volume relative to He or thoracic imaging despite adherence to current recommendations for proper measurement technique....
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PMID: 20022972
PDF is available here.
PDF is available here.
Clinical Biochemistry (43)6 2010
Abstract
HbA(1c) results became falsely elevated when samples were diluted in hemolysis reagent, but not in water. QC-procedures failed to detect this error as calibrator and QC samples were manually diluted in water, according to manufacturer's instructions, whereas patient samples were automatically dilute...
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PMID: 20060821
PDF is available here.
PDF is available here.
Abstract
Comparability of obtained results confirmed the applicability of recently recommended "common" reference intervals to the Croatian population for all laboratories measuring serum creatinine concentrations using enzymatic methods traceable to the IDMS method and NIST SRM 967....
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PMID: 19943808
PDF is available here.
PDF is available here.
ASAIO Journal (56)4 2010
Abstract
BMC Pulmonary Medicine (10)1 2010
Abstract
Total protein and capture FRET activity assays were developed that were sensitive enough to detect MMP-12 in 37 of 38 donor sputum samples. A comparison of results between the two assays shows that a majority of sputum MMP-12 is in the active form. No differences were seen between normal, asthmatic,...
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PMID: 20678199
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
This study compared nine susceptibility testing methods and 12 endpoints for anidulafungin, caspofungin, and micafungin with the same collection of blinded FKS hot spot mutant (n = 29) and wild-type isolates (n = 94). The susceptibility tests included EUCAST Edef 7.1, agar dilution, Etest, and disk...
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PMID: 19884370
PDF is available here.
PDF is available here.
Abstract
We determine the Taylor dispersion coefficient that describes the degree of axial mixing for the MID models, while accounting for the presence of red blood cells in the capillaries. The capillaries are treated as well mixed with no radial concentration gradients. The concentration in tissue is treat...
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PMID: 19318591
PDF is available here.
PDF is available here.
Abstract
TG-ROC analysis can provide important information about diagnostic tests, in addition to offering suggestions on cut-off points that can improve test sensitivity and specificity estimates and assessing these tests in terms of the best cost-benefit ratio....
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PMID: 19851639
PDF is available here.
PDF is available here.
Abstract
British Journal of Nutrition (102)12 2009
Abstract
Gambian infants show growth faltering, but the underlying body composition is unknown. The present study aimed to compare body composition in Gambian and UK infants using 2H dilution; and to evaluate accuracy of bioelectrical impedance analysis (BIA) and creatinine excretion for estimating lean mass...
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PMID: 19682404
PDF is available here.
PDF is available here.
Analytical Biochemistry (398)2 2009
Abstract
New stable isotope dilution assays were developed for the simultaneous quantitation of the folates 5-methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid, tetrahydrofolic acid, 10-formylfolic acid and folic acid as well as for their catabolites para-aminobenzoylglutamate (pABG) a...
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PMID: 19903444
PDF is available here.
PDF is available here.
Analytical Biochemistry (395)1 2009
Abstract
A gas chromatography-mass spectrometry (GC-MS) method for the simultaneous measurement of isotopic enrichment and concentration of guanidinoacetate (GAA) and creatine in plasma sample for kinetic studies is reported. The method, based on preparation of the bis(trifluoromethyl)pyrimidine methyl ester...
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PMID: 19646413
PDF is available here.
PDF is available here.
Abstract
A sphingosine kinase inhibitor reduced cell colony formation and activated caspase-3 in both TMZ-sensitive and resistant GBM cells. CONCLUSION: Addition of a sphingosine kinase inhibitor to the standard chemotherapy regimen against GBM may be beneficial....
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PMID: 19597728
PDF is available here.
PDF is available here.
Analytical Biochemistry (395)1 2009
Abstract
We developed a protein isoform analysis method utilizing Stable Isotope Labeling Tandem Mass Spectrometry (SILT MS). ApoE isoforms are quantitated using the intensities of the b and y ions of the (13)C-labeled tryptic isoform-specific peptides versus unlabeled tryptic isoform-specific peptides. The...
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PMID: 19653990
PDF is available here.
PDF is available here.
Decoding the key aroma compounds of a Hungarian-type salami by molecular sensory science approaches.
Abstract
Application of aroma extract dilution analysis on an extract/distillate prepared from a Hungarian-type salami and subsequent identification experiments led to the identification of 51 of 55 odor-active compounds detected in the flavor dilution (FD) factor range of 16-4096. Nineteen of these compounds...
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PMID: 19358532
PDF is available here.
PDF is available here.
Microcirculation (16)3 2009
Abstract
Our analysis raises two questions about 1) the estimation of the concentration of the tracer (dextran 40) at zero time and 2) the estimation of plasma volume, both of which can be answered by changes in experimental protocol. A third question, concerning the partition coefficient of the tracer betwe...
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PMID: 19184776
PDF is available here.
PDF is available here.
Abstract
The antibacterial and synergistic activity of the ethanol extract from Hyptis martiusii Benth. was assayed by microdillution. The growth of two isolates of Escherichia coli tested was inhibited by the extract. The minimum inhibitory and minimum bactericidal concentrations (MIC and MBC) values ranged...
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PMID: 19675386
PDF is available here.
PDF is available here.
Lab On a Chip (9)5 2009
Abstract
We propose a generalized serial dilution module for universal microfluidic concentration gradient generators including N cascaded-mixing stages in a stepwise manner. Desired concentrations were generated by means of controlled volumetric mixing ratios of two merging solutions in each stage. The flow...
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PMID: 19224022
PDF is available here.
PDF is available here.
Intensive Care Medicine (35)3 2009
Abstract
To achieve a good precision with this technique, three lithium dilution measurements should be averaged. This will allow changes in cardiac output of more than 14% to be reliably detected. The understanding of the precision of this technique allows the user to know when a real change has happened to...
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PMID: 18802681
PDF is available here.
PDF is available here.
Annals of Hematology (88)2 2009
Abstract
We detected typical NPM1 mutations (types A, B, D) in untreated Chinese AML patients using real-time quantitative polymerase chain reaction (RQ-PCR) followed by sequence analysis. The detection rate of NPM1 mutations in 220 AML patients was 16.4%, including 107 (14.2%) with the French-American-Briti...
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PMID: 18726096
PDF is available here.
PDF is available here.
Abstract
An isotope dilution method using [1-(13)C]sodium (Na) acetate was conducted to determine the effect of feeding hop (Humulus lupulus L.) residues on plasma acetate metabolism in six adult crossbred sheep. The sheep were fed 63 g/kg BW(0.75)/d of either mixed hay (MH-diet) of orchardgrass (Dactylis gl...
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PMID: 19365576
PDF is available here.
PDF is available here.
Abstract
The Journal of the Louisiana State Medical Society : official organ of the Louisiana State Medical Society (161)5 2009
Abstract
This study compared the accuracy of a formula calculating cardiac output utilizing a patient size modification of a pulse pressure formula of Starr with that measured with a standard thermal dilution technique. During a six-month period 111 patients in the intensive care unit (ICU) on the cardiothor...
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PMID: 19927944
PDF is available here.
PDF is available here.
BJU International (102)11 2008
Abstract
There is significant variation in the BSA calculated by commonly used formulae. This variation is carried over and further magnified in the sequential calculation of PV and PSA mass. Hence arbitrary selection of BSA and PV formulae is likely to affect inferences....
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PMID: 18691174
PDF is available here.
PDF is available here.
Abstract
An isotope dilution method has been developed for the speciation analysis of chromium in natural waters which accounts for species interconversions without the requirement of a separation instrument connected to the mass spectrometer. The method involves (i) in-situ spiking of the sample with isotop...
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PMID: 18804619
PDF is available here.
PDF is available here.
Abstract
A biosensor for determination of oxalate concentration in urine has been developed by immobilisation of oxalate oxidase and peroxidase on the surface of an interdigitated gold electrode. Enzyme immobilisation was performed using BSA and glutaraldehyde. Biamperometric measurements were made in flow c...
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PMID: 18804624
PDF is available here.
PDF is available here.
Abstract
Abstract
Twenty-five Ab were Hr-CP (growth of resistant colonies within the inhibition area of disks and E-test strips). Agreement for the MICs (+/- 1log(2)) in Hr-CP colonies was as follows: Wider vs. MD (96% IMP, 100% MPM), Wider vs. E-test (50% IMP, 64% MPM), MD vs. E-test (64% IMP, 60% MPM). Major errors...
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PMID: 19094860
PDF is available here.
PDF is available here.
Abstract
The Fick and thermodilution (TD) methods are two currently popular techniques for determination of cardiac output (CO) in adult horses. To our knowledge, a comparison of these two techniques has not been reported. Six healthy, resting, fit, adult horses of either sex and weighing 516.5+/-33.2 kg (me...
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PMID: 18093624
PDF is available here.
PDF is available here.
Abstract
An analytical method for the measurement of five naturally occurring bromophenols of sensory relevance in seafood (barramundi and prawns) is presented. The method combines simultaneous distillation-extraction followed by alkaline back extraction of a hexane extract and subsequent acetylation of the...
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PMID: 18754626
PDF is available here.
PDF is available here.
Abstract
To provide a brief overview of the main techniques to measure muscle blood flow in humans and highlight some of the strengths and weaknesses associated with each technique.
Peak muscle blood flow values of 300 ml/min per 100 g are possible in humans during heavy exercise performed with small muscle...
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PMID: 18685453
PDF is available here.
PDF is available here.
Abstract
Polyaromatic hydrocarbons (PAHs) are a group of environmental toxicants mainly emitted from diffuse sources. Fixed wavelength fluorescence of fish bile is a simple screening method that allows environmental monitoring of PAH exposure to fish. One drawback with this method is that the results can be...
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PMID: 17899412
PDF is available here.
PDF is available here.
Clinical Chemistry (54)9 2008
Abstract
Abstract
A method for the simultaneous quantitation of total glutathione and total cysteine in wheat flour by a stable isotope dilution assay using high-performance liquid chromatography/tandem mass spectrometry (HPLC-MS/MS) was developed. As internal standards, L-[(13)C3, (15)N]cysteine and L-gamma-glutamyl...
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PMID: 18656940
PDF is available here.
PDF is available here.
Abstract
Dithiocarbamates (DTCs) can be formed by the in situ condensation of polar alkylamines with CS 2, and assembled into dithiocarbamate-anchored monolayers (DAMs) on Au substrates in aqueous solutions. Primary and secondary amines can both be used to prepare DTCs, but have significant differences in th...
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PMID: 18616309
PDF is available here.
PDF is available here.
Abstract
We report the effect of surface coverage and sequence on the performance of 5' thiolated, 3' fluorophore-labeled DNA hairpin probes bound to Au/Ag striped ("barcoded") metal nanowires. Coverage was controlled by varying probe concentration, buffer ionic strength, and by addition of short hydroxy-ter...
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PMID: 18624416
PDF is available here.
PDF is available here.
Analytica Chimica Acta (623)2 2008
Abstract
We describe a robust and simple method to measure dissolved iron (DFe) concentrations in seawater down to <0.1 nmol L(-1) level, by isotope dilution multiple collector inductively coupled plasma mass spectrometry (ID-MC-ICP-MS) using a (54)Fe spike and measuring the (57)Fe/(54)Fe ratio. The method p...
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PMID: 18620916
PDF is available here.
PDF is available here.
Abstract
On the basis of a stable isotope dilution assay and derivatization with 2-mercaptobenzoic acid, the presence of the carcinogenic glycidamide ( 2) in processed foods was verified for the first time. Using (13)C-labeled 2 as the internal standard and the formation of the thioether derivatives, a new s...
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PMID: 18624425
PDF is available here.
PDF is available here.
Abstract
The isotope dilution headspace solid-phase microextraction and gas chromatography-mass spectrometry method for bisphenol A (BPA) developed previously was used successfully in a BPA migration study at 70 degrees C of polycarbonate baby and reusable water bottles recently sold in Canada by using the w...
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PMID: 18636679
PDF is available here.
PDF is available here.
Abstract
I (5-MTHF >95%; THF <5%; 5,10-MTHF <5%), type II (5-MTHF <95%; THF 5% to 20%; 5,10-MTHF <5%), type III (5-MTHF >55%; THF >20%; 5,10-MTHF >5%), and type IV (5-MTHF <55%; THF >20%; 5,10-MTHF >5%).
Copyright (c) 2008 John Wiley & Sons, Ltd....
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PMID: 18634122
PDF is available here.
PDF is available here.
Abstract
Overall, although good between-method agreement was found across the 3 groups, the degree of agreement varied according to subject characteristics, particularly at the extremes of ECW and body weight. We advance a possible mechanism that may link subject characteristics with the degree of agreement...
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PMID: 18689366
PDF is available here.
PDF is available here.
Abstract
A micro-dilution technique with changes in optical density (OD) used to measure bacterial growth over 72 h in culture media containing 0, 5, 10, 20, 40, 80 and 160 microg OTC (oxytetracycline)ml(-1) was applied to determine growth inhibition (GI) in mixed bacterial strains cultured from samples of m...
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PMID: 18589456
PDF is available here.
PDF is available here.
Clinical Chemistry (54)8 2008
Abstract
This study demonstrated fairly good accuracy and standardization of the tested ID-LC-MS/MS procedures. Performance differences between procedures were evident in some instances, due to improper calibration and between-run calibration control. This emphasizes the need for thorough validation, includi...
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PMID: 18556330
PDF is available here.
PDF is available here.
Abstract
Thirty-one of the 45 odor-active compounds previously identified by us in an American Bourbon whisky were quantified by stable isotope dilution assays. Also for this purpose, new synthetic pathways were developed for the synthesis of the deuterium-labeled whisky lactone as well as for gamma-nona- an...
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PMID: 18582086
PDF is available here.
PDF is available here.
Abstract
A new isotope dilution gas chromatography/chemical ionization/tandem mass spectrometric method was developed for the analysis of carcinogenic hydrazine in drinking water. The sample preparation was performed by using the optimized derivatization and multiple liquid-liquid extraction techniques. Usin...
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PMID: 18564853
PDF is available here.
PDF is available here.
Abstract
Abstract
A method of calibrating unquantified samples was developed in order to conduct tests from approximately 65 microl or less of self-collected whole blood from the finger. In this method, the dilution ratio is estimated by absorption analysis of an internal standard substance in the blood dilution buff...
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PMID: 18709989
PDF is available here.
PDF is available here.
Analytical Biochemistry (378)1 2008
Abstract
We describe an algorithm, Q-Anal, that blends elements from current methods to by-pass assumptions regarding PCR efficiency and improve the threshold selection process to minimize error in comparative qPCR analysis. This algorithm uses iterative linear regression to identify the exponential phase fo...
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PMID: 18420021
PDF is available here.
PDF is available here.
Analytical Biochemistry (378)1 2008
Abstract
An electrochemical immunosensor is reported by using aptamer-based enzymatic amplification with immunoglobulin E (IgE) as the model analyte. In this method, the IgE antibody is covalently immobilized as the capture probe on the gold electrode via a self-assembled monolayer of cysteamine. After the t...
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PMID: 18423388
PDF is available here.
PDF is available here.
Analytical Biochemistry (378)1 2008
Abstract
A simple electrochemiluminescence-based assay for RNA N-glycosidase activity has been modified to permit its use with authentic extracts of Ricinus communis (castor beans) and Abrus precatorius (jequirity seeds)--the natural sources of ricin and abrin. Modifications include the addition of an RNase...
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PMID: 18394420
PDF is available here.
PDF is available here.
Abstract
A series of equations are developed for the study of the effects of cosolvents on the solubility of a solute in mixed solutions where the solute displays a finite solubility. The equations differ depending on the scale used for the solute (and cosolvent) concentrations. The expressions use Kirkwood-B...
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PMID: 18529024
PDF is available here.
PDF is available here.
Abstract
Direct monitoring of primary molecular-binding interactions without the need for secondary reactants would markedly simplify and expand applications of high-throughput label-free detection methods. A simple interferometric technique is presented that monitors the optical phase difference resulting f...
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PMID: 18523019
PDF is available here.
PDF is available here.