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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Micromanipulation (3):
Recent article
Micromanipulation
Abstract
We propose to use a robot-tweezer manipulation system to solve the problem of automatic transportation of biological cells, where optical tweezers function as special robot end effectors. Dynamics equation of the cell in optical tweezers is analyzed. A closed-loop controller is designed for transpor...
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PMID: 21764021
PDF is available here.
PDF is available here.
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Abstract
The production of acoustical vortices-based potential wells for particle trapping is not only restricted to the use of a Laguerre-Gaussian beam. Other useful types of vortex beams include an r-vortex beam, a non-diffracting high-order Bessel and Bessel-Gauss beam, a fractional (diffr...
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PMID: 21429858
PDF is available here.
PDF is available here.
Optics Letters (35)12 2010
Abstract
We develop light-driven optoelectronic tweezers based on the organic photoconductive material titanium oxide phthalocyanine. These tweezers function based on negative dielectrophoresis (nDEP). The dynamic manipulation of a single microparticle and cell patterning are demonstrated by using this light...
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PMID: 20548352
PDF is available here.
PDF is available here.
Lab On a Chip (10)11 2010
Abstract
This paper presents a handheld mechanical cell lysis chip with ultra-sharp nano-blade arrays fabricated by simple and cost effective crystalline wet etching of (110) silicon. The ultra-sharp nano-blade array is simply formed by the undercutting of (110) silicon during the crystalline wet etching pro...
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PMID: 20480109
PDF is available here.
PDF is available here.
Lab On a Chip (10)11 2010
Abstract
We provide successful examples of biomechanical and biochemical assays on living human leukocytes passing through 4 mum wide capillaries. The blebbing process dynamics are monitored by conventional 24 fps videomicroscopy and subcellular cytoskeleton organization is imaged by on-chip immunostaining....
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PMID: 20480111
PDF is available here.
PDF is available here.
Lab On a Chip (10)11 2010
Abstract
We present an extended computer simulation method which allows in silico design and testing of guiding tracks for molecular shuttles powered by kinesin motors. The simulation reproduced molecular shuttle movements under external forces and dissociation of shuttles from guiding track surfaces. The si...
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PMID: 20480110
PDF is available here.
PDF is available here.
Lab On a Chip (10)11 2010
Abstract
We present a microfluidic sample introduction approach based on a novel flow control mechanism, hydrodynamic gated injection. It has the advantages of easy and flexible flow control, similar to its analog, electrokinetic gated injection, but exhibits the unique properties of strong driving force and...
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PMID: 20480113
PDF is available here.
PDF is available here.
Abstract
We propose an acoustics-vortex- based trapping model of acoustic tweezers. A four-element 1-MHz planar transducer was used to generate 1-MHz sine waves at 1 MPa, with adjacent elements being driven with a pi/2-rad phase difference. Each element was a square with a side length of 5.08 mm, with kerfs...
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PMID: 20529720
PDF is available here.
PDF is available here.
Nanotechnology (21)16 2010
Abstract
Size-based separations technologies will inevitably benefit from advances in nanotechnology. Direct-write nanofabrication provides a useful mechanism for depositing/etching nanoscale elements in environments otherwise inaccessible to conventional nanofabrication techniques. Here, electron beam induc...
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PMID: 20351412
PDF is available here.
PDF is available here.
Abstract
In this paper, methods to measure viscoelastic properties of time-dependent materials are proposed using sharp, flat, and buckling tips inside an environmental SEM. Single W303 yeast cells were employed in this study. Each of the tips was used to indent single cells in a nanoindentat...
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PMID: 19887332
PDF is available here.
PDF is available here.
Abstract
Mechanical signal is important for regulating cellular activities, including proliferation, metabolism, matrix production, and orientation. Bioreactors with loading functions can be used to precondition cells in three-dimensional (3D) constructs so as to study the cellular responses to mechanical st...
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PMID: 19368498
PDF is available here.
PDF is available here.
Orvostorteneti kozlemenyek (56)1-4 2010
Abstract
Tibor Péterfi (1883-1953) was an eminent and internationally renowned biologist. He made great advances in the field of experimental physiology focusing his cytological research on microscopic examination of living cells. For this task, he created a tool named micromanipulator basin...
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PMID: 21661264
PDF is available here.
PDF is available here.
Abstract
Atomic force microscope (AFM) has been used incrementally over the last decade in cell biology. Beyond its usefulness in high resolution imaging, AFM also has unique capabilities for probing the viscoelastic properties of living cells in culture and, even more, mapping the spatial distribution of ce...
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PMID: 20463929
PDF is available here.
PDF is available here.
Abstract
Abstract
We analyze the impact of the specific manipulation of the intracellular refractive index by microinjection on the DHM phase contrast. Glycerol is chosen as osmolyte, which combines high solubility in aqueous solutions and biological compatibility. We show that the intracellular injection of glycerol...
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PMID: 20799787
PDF is available here.
PDF is available here.
Abstract
We present a review of optical manipulation, emphasizing its impact on the areas of single-molecule studies and single-cell biology, and indicating some of the key experiments in the fields....
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PMID: 20799781
PDF is available here.
PDF is available here.
Abstract
We demonstrate a single-beam, high-throughput method where optical forces are applied anisotropically to stretch swollen erythrocytes in microfluidic flow. We also present numerical simulations of model spherical elastic cells subjected to optical forces and show that dual, opposing optical traps ar...
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PMID: 20799841
PDF is available here.
PDF is available here.
Abstract
The detection and trapping of single fluorescent molecules in solution within a nanochannel is studied using numerical simulations. As optical forces are insufficient for trapping molecules much smaller than the optical wavelength, a means for sensing a molecule's position along the nanochannel and...
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PMID: 20799801
PDF is available here.
PDF is available here.
Methods in Enzymology (476) 2010
Abstract
Fertilization by intracytoplasmic sperm injection (ICSI) is a powerful technique that can be used to understand better the biology of fertilization, in addition to a form of assisted reproduction both in humans and in endangered species. Mouse is often the model organism of choice to study mammalian...
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PMID: 20691870
PDF is available here.
PDF is available here.
Methods in Enzymology (476) 2010
Abstract
Many experimental approaches to answer questions about mammalian development begin with the isolation of mouse gametes and/or embryos. Here, methods used to isolate and store growing and fully-grown oocytes, ovulated (unfertilized) eggs, cleavage-stage embryos, and sperm from mice are described. Pro...
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PMID: 20691861
PDF is available here.
PDF is available here.
Methods in Enzymology (476) 2010
Abstract
We describe a new, more efficient mouse cloning protocol using histone deacetylase inhibitor (HDACi), which increases the success rates of cloned mice or establish rate of ES cells to fivefold.
Copyright (c) 2010 Elsevier Inc. All rights reserved....
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PMID: 20691865
PDF is available here.
PDF is available here.
Methods in Enzymology (476) 2010
Abstract
We review technical aspects of both in utero and exo utero surgical approaches and how these surgeries are used in conjunction with other experimental applications.
Copyright (c) 2010 Elsevier Inc. All rights reserved....
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PMID: 20691868
PDF is available here.
PDF is available here.
Abstract
The results of pioneering studies on the development of radically new noninvasive methods for the transplantation of mammalian somatic cell nuclei with the use of optical laser manipulations are presented, and their comparison with traditional invasive methods is performed. It is shown that all the...
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PMID: 20586321
PDF is available here.
PDF is available here.
Abstract
We can expect that after fertilization, the embryos arising from these defective oocytes may not develop or that their development might eventually be compromised. For this reason, an increased effort to determine how to repair oocytes is evident in the literature. In general, abnormalities (defects...
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PMID: 20075602
PDF is available here.
PDF is available here.
Abstract
We discuss what entails a quiet environment--what sources of noise are relevant and how they affect the instrument--and provide guidelines for choosing an appropriate location for a high-resolution instrument....
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PMID: 20147037
PDF is available here.
PDF is available here.
Abstract
We present the design of a high-resolution optical tweezers, which is designed to minimize sensitivity to environmental noise. When the tether is attached to a stage, resolution is typically limited by drift and fluctuations in the relative position of the stage and trap-forming objective, cued by e...
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PMID: 20147038
PDF is available here.
PDF is available here.
Abstract
We focus on the ways in which the design and implementation of an experiment--the choice of bead size, tether length, and even the method in which the motions of the beads are monitored--can have a large impact on the fundamental resolution of the measurement. By investigating how the Brownian fluct...
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PMID: 20147040
PDF is available here.
PDF is available here.
Abstract
We briefly review the basic concepts and components of optical traps and the single-molecule experiments in which they are used....
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PMID: 20147062
PDF is available here.
PDF is available here.
Optics Express (17)20 2009
Abstract
This paper presents a decent polymer material for fabricating optically-induced dielectrophoretic (ODEP) devices, which can manipulate microparticles or cells by using moving light patterns. A thin film of a bulk-heterojunction (BHJ) polymer, a mixture of regioregular poly(3-hexylthiophene) and [6,6...
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PMID: 19907545
PDF is available here.
PDF is available here.
Abstract
We report the rotational maneuver of ferromagnetic nanowires and their applications in cell manipulation. The rotational maneuver is studied under two different suspension conditions. The rotation of nanowires in the fluid is analyzed using Stokes flow assumption. Experimental results show that when...
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PMID: 20051338
PDF is available here.
PDF is available here.
Abstract
We have used live-cell imaging and micromanipulation to determine precisely the karyotype of the orb-weaving spider Neoscona arabesca (Walckenaer). We have found that N. arabesca has a sex determination mechanism of XO (male)-XX (female) with 2n = 20 autosomes. Staining of female tissues revealed a...
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PMID: 19765098
PDF is available here.
PDF is available here.
Abstract
We propose a microelectrode array (MEA) featuring thickened microelectrodes with vertical sidewalls (VSW) to focus electrical fields horizontally on targets positioned in between paired electrodes. These microelectrodes were fabricated using gold electroplating that was molded by photolithographical...
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PMID: 19535240
PDF is available here.
PDF is available here.
Optics Express (17)16 2009
Abstract
Optical tweezers provide a versatile tool in biological and physical researches. Optical tweezers based on optical fibers are more flexible and ready to be integrated when compared with those based on microscope objectives. In this paper, the three-dimensional (3D) trapping ability of an inclined du...
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PMID: 19654770
PDF is available here.
PDF is available here.
Abstract
Studies of single cells via microscopy and microinjection are a key component in research on gene functions, cancer, stem cells, and reproductive technology. As biomedical experiments become more complex, there is an urgent need to use robotic systems to improve cell manipulation and microinjection...
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PMID: 19605307
PDF is available here.
PDF is available here.
British Journal of Nutrition (102)1 2009
Abstract
The present experiment aimed to study the influence of six sources of non-digestible carbohydrates (NDC) on the mucous layer in the colon of rats. The NDC sources used were as follows: cellulose (C); pectin (P); inulin; resistant starch (RS); barley hulls. The diets contained 108-140 g NDC/kg DM. A...
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PMID: 19138435
PDF is available here.
PDF is available here.
Nanotechnology (20)21 2009
Abstract
We introduce optimized handling conditions and investigate the physical properties of the cantilever-tip-sample ensemble, which is essential for the interpretation of the experimental data gained from this technique. In contrast to conventional AFM methods, TREC is based on a more sophisticated feed...
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PMID: 19423924
PDF is available here.
PDF is available here.
Nanotechnology (20)21 2009
Abstract
A conventional atomic force microscope (AFM) has been successfully applied to manipulating nanoparticles (zero-dimensional), nanowires (one-dimensional) or nanotubes (one- or two-dimensional) by widely used pushing or pulling operations on a single surface. However, pick-and-place nanomanipulation i...
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PMID: 19423927
PDF is available here.
PDF is available here.
Nanotechnology (20)18 2009
Abstract
We explore a new type of DNA translocation. We dub it 'reverse DNA translocation', in which the DNA is pulled through a nanopore mechanically by a magnetic bead, driven by a magnetic-field gradient. This technique is compatible with simultaneous ionic current measurements and is suitable for multipl...
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PMID: 19420602
PDF is available here.
PDF is available here.
Optics Express (17)8 2009
Abstract
We describe open-loop and closed-loop multiplexed force measurements using holographic optical tweezers. We quantify the performance of our novel video-based control system in a driven suspension of colloidal particles. We demonstrate our system's abilities with the measurement of the mechanical cou...
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PMID: 19365444
PDF is available here.
PDF is available here.
Proceedings of the Institution of Mechanical Engineers, Part H: Journal of Engineering in Medicine (223)3 2009
Abstract
Atomic force microscopy (AFM) techniques have been increasingly used for investigating the mechanical properties of articular cartilage. According to the previous studies reporting the microscale Young's modulus under AFM indentation tests, the Hertz contact model has been employed with a sharp coni...
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PMID: 19405439
PDF is available here.
PDF is available here.
Biomedical Microdevices (11)2 2009
Abstract
This paper reports a monolithic, force-feedback MEMS (microelectomechanical systems) microgripper and its application to micro-scale compression testing of swollen hydrogel microcapsules at wet state during manipulation. The single-chip microgripper integrates an electrothermal microactuator and two...
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PMID: 19015993
PDF is available here.
PDF is available here.
Optics Express (17)7 2009
Abstract
We suggest a novel approach in all-optical trapping employing a photophoretic force for manipulation of absorbing particles in open air. We demonstrate experimentally the robust three-dimensional guiding, over the distances of a few millimeters, of agglomerates of carbon nanoparticles with the size...
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PMID: 19333344
PDF is available here.
PDF is available here.
Optics Express (17)7 2009
Abstract
We describe an approach that applies optical scattering forces to translate varied sized particles differentially from a surface followed by the introduction of an optical standing wave to maintain and tighten the positional tolerance of the differentiated particles in the medium. Numerical simulati...
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PMID: 19333297
PDF is available here.
PDF is available here.
Optics Express (17)7 2009
Abstract
We present trap profile measurements for HeLa cells in Optoelectronic Tweezers (OET) based on a data projector. The data projector is used as a light source to illuminate amorphous Si creating virtual electrodes which are used to trap particles through dielectrophoresis. We show that although the tr...
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PMID: 19333286
PDF is available here.
PDF is available here.
Methods in Molecular Biology (530)530 2009
Abstract
We describe the steps and procedures required to generate the chimeric mice leading to the transfer of a genetic mutation to the mouse germline....
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PMID: 19266343
PDF is available here.
PDF is available here.
Biology of Reproduction (80)3 2009
Abstract
It is still difficult to successfully cryopreserve in vitro-produced (IVP) swine embryos, as they are sensitive to chilling due to the abundance of intracellular lipids. Mechanical delipation through micromanipulation is successful, but this method increases the potential of pathogen transmission be...
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PMID: 19038857
PDF is available here.
PDF is available here.
Abstract
A new self-calibration method for a piezoelectric actuator-based vision-guided cell micromanipulation system is proposed. This method consolidates all the system parameters' uncertainties into a matrix instead of classifying into intrinsic and extrinsic. The position difference of the micromanipulat...
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PMID: 19220701
PDF is available here.
PDF is available here.
Physical Review E (79)2 Pt 1 2009
Abstract
We report an alternative microfluidic approach to studying the motion of single DNA molecules in an electric field. Making use of a closely fitting droplet in a microchannel, DNA molecules can be confined within the submicrometer film beneath the droplet. Several dynamic events at the single-molecul...
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PMID: 19391772
PDF is available here.
PDF is available here.
Abstract
Ultrasonics (49)1 2009
Abstract
Handling of micrometer sizes particles, such as biological cells or coated beads, plays a relevant role in the field of life science. A number of devices have been presented in the last years, in which acoustic forces generated by coupling the vibration of a solid structure excited by a piezoelectri...
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PMID: 18590923
PDF is available here.
PDF is available here.
Ultrasonics (49)1 2009
Abstract
We demonstrate and investigate multiple localized ultrasonic manipulation functions in series in microfluidic chips. The manipulation functions are based on spatially separated and confined ultrasonic primary radiation force fields, obtained by local matching of the resonance condition of the microf...
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PMID: 18701122
PDF is available here.
PDF is available here.
Abstract
We have developed a software to assist the operator in the accurate assembly of microstructures in a SEM, in which computer-generated outlines of the target structure [graphical templates (GTs)] are superimposed on the monitor. The displayed GTs are distorted on the basis of the image properties of...
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PMID: 19191465
PDF is available here.
PDF is available here.
Abstract
We demonstrate the use of patterning flows produced by two different sorts of grooved surfaces: single slanted groove series (for enrichment patterns) and V-shaped groove series (for focusing patterns), into a microfluidic device to continuously manipulate the flowing particles, including microbeads...
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PMID: 19640141
PDF is available here.
PDF is available here.
International Conference of the IEEE Engineering in Medicine and Biology Society. Proceedings (2009) 2009
Abstract
Tissue engineering has the potential to improve the current methods for replacing organs and tissues and for investigating cellular process within the scope of a tissue test system. Bioprinting technology can aid in the difficult task of arranging live mammalian cells and biomaterial...
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PMID: 19963663
PDF is available here.
PDF is available here.
Integrative Biology (1)3 2009
Abstract
Bio-electrospraying, a recently discovered, direct electric field driven cell engineering process, has been demonstrated to have no harmful effects on treated cells at a molecular level. Although several cell types from both immortalized and primary cultures have been assessed post-t...
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PMID: 20023737
PDF is available here.
PDF is available here.
Abstract
We use micropipette aspiration to induce large, local deformations in the nucleus, and microscopy to image nuclear shape as well as the response of fluorescently labeled components in the inner nucleus (chromatin and nucleoli) and the nuclear envelope (lamins and membranes). By monitoring the respon...
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PMID: 18951176
PDF is available here.
PDF is available here.
Abstract
Although a common approach in large vertebrate embryos such as chick or frog, manipulation at the tissue level is only rarely applied to zebrafish embryos. Despite its relatively small size, the zebrafish embryo can be readily used for micromanipulations such as tissue and organ primordium transplan...
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PMID: 19378104
PDF is available here.
PDF is available here.
Abstract
We reconfigured for nondescanned 2-P imaging, a dedicated electrophysiology microscope, the Nikon FN1. This microscope is compact, with retractable objectives, allowing more stage space. The instrument is fitted with long-working-distance objectives (2.5- to 3.5-mm WD) with a narrow bore, high NA, a...
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PMID: 19405761
PDF is available here.
PDF is available here.
Abstract
This paper reports a new microfluidic device capable of performing optically induced flow cytometry (OIFC). This enables it to continuously count and to sort microparticles based on optically induced dielectrophoretic (ODEP) forces. Gravity was used to drive the particles instead of using syringe pu...
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PMID: 18635347
PDF is available here.
PDF is available here.
Abstract
Uniform patterning of cells is highly desirable for most cellular studies involving cell-cell interactions but is often difficult in an in vitro environment. This paper presents the development of a collagen-coated planar interdigitated ring electrode (PIRE) array utilizing positive dielectrophoresi...
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PMID: 18760911
PDF is available here.
PDF is available here.