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Biosensing Techniques (1):
Recent article
Biosensing Techniques
Abstract
A sensitive enzymeless organophosphate pesticides (OPs) sensor is fabricated by using Au nanoparticles (AuNPs) decorated graphene nanosheets (GNs) modified glassy carbon electrode as solid phase extraction (SPE). Such a nanostructured composite film, combining the advantages of AuNPs...
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PMID: 21807193
PDF is available here.
PDF is available here.
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Biomacromolecules (12)9 2011
Abstract
We propose substrate-independent modification for creating a protein-repellent surface based on dopamine-melanin anchoring layer used for subsequent binding of poly(ethylene oxide) (PEO) from melt. We verified that the dopamine-melanin layer can be formed on literally any substrate and could serve a...
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PMID: 21823677
PDF is available here.
PDF is available here.
Abstract
We show that a phosphorylation gradient is produced by Aurora B concentration and activation at centromeres and release and diffusion to reach substrates at a distance. Kinase concentration, either at centromeres or at another chromosomal site, is necessary for activity globally. By experimentally m...
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PMID: 21844210
PDF is available here.
PDF is available here.
Abstract
We previously reported (Koshi, Y.; Nakata, E.; Miyagawa, M.; Tsukiji, S.; Ogawa, T.; Hamachi, I. J. Am. Chem. Soc.2008, 130, 245.), here we have developed a new organocatalyst-based approach that allows specific chemical acylation of a receptor protein on the surface of live cells. The catalysts con...
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PMID: 21761940
PDF is available here.
PDF is available here.
Abstract
We proposed a "solidified liquid layer" model that enabled QCM to be used as a biomolecular ruler. Here we applied five kinds of proteins with significant differences in their sizes and shapes to further validate this model. The effective thickness (T(eff)) of surface immobilized, hydrated proteins...
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PMID: 21093226
PDF is available here.
PDF is available here.
Abstract
We successfully detected a low concentration of cocaine (300 ng/mL, the drug test cutoff limit) within 60 s using a biological nanopore embedded in a microchip....
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PMID: 21553872
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
We describe a novel, sensitive, homogenous high throughput reporter-based in vitro assay for SUMO protease activity developed by Progenra, Inc. A reporter construct was created by fusing His(6)-tagged small ubiquitin-like modifier (SUMO) to the amino terminus of the reporter enzyme phospholipase A(2...
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PMID: 19107424
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
The immobilization procedure to a biosensor surface has a major influence on the measurement results. To characterize the immobilization onto various biolayers, the interaction of DNA anti-thrombin aptamer with the protein thrombin was used as a model system. The aptamer was immobilized to a two-dim...
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PMID: 19377993
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Many biosensors depend on molecular recognition reagents to achieve highly sensitive and specific detection levels of an analyte of interest. Although new and improved detection platforms continue to be developed, improvements in the affinity and specificity of the molecular recognition reagents oft...
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PMID: 19159106
PDF is available here.
PDF is available here.
Abstract
A novel method for high-speed fabrication of large scale periodic arrays of nanoparticles (diameters 40-200 nm) is developed. This method is based on a combination of nanosphere lithography and laser-induced transfer. Fabricated spherical nanoparticles are partially embedded into a p...
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PMID: 21539373
PDF is available here.
PDF is available here.
Abstract
We propose the functionalization of MNPs via a two-steps strategy that takes advantage of the ionic reversible interactions between the Ab and the MNP. These interactions make possible the orientation of the Ab on the MNP surface before being attached in an irreversible way via covalent bonds. Three...
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PMID: 21526783
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)9 2011
Abstract
Multivalent protein binding plays an important role not only in biological recognition but also in biosensor preparation. Infrared reflection absorption spectroscopy and surface plasmon resonance techniques have been used to investigate concanavalin A (Con A) binding to binary monolayers composed of...
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PMID: 21640072
PDF is available here.
PDF is available here.
Abstract
I monoclonal antibodies (Mabs) were prepared by hybridoma technique and firstly used to develop a Sudan I immunosensor by immobilizing the Mabs on a gold electrode. o-Mercaptobenzoic acid (MBA) was covalently conjugated on the gold electrode to form a self-assembled monolayer (SAM). The immobilizati...
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PMID: 21315921
PDF is available here.
PDF is available here.
Abstract
We investigated whether heparin interferes with HMGB1/RAGE interaction and prevents the cytokine activity. We used fluorescence spectrometry, circular dichroism spectrometry and SPR biosensor technique to evaluate the effect. After treatment of HMGB1 with different concentrations of heparin (0, 50,...
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PMID: 21095260
PDF is available here.
PDF is available here.
Abstract
We investigated whether heparin interferes with HMGB1/RAGE interaction and prevents the cytokine activity. We used fluorescence spectrometry, circular dichroism spectrometry and SPR biosensor technique to evaluate the effect. After treatment of HMGB1 with different concentrations of heparin (0, 50,...
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PMID: 21095260
PDF is available here.
PDF is available here.
Abstract
We investigated whether heparin interferes with HMGB1/RAGE interaction and prevents the cytokine activity. We used fluorescence spectrometry, circular dichroism spectrometry and SPR biosensor technique to evaluate the effect. After treatment of HMGB1 with different concentrations of heparin (0, 50,...
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PMID: 21095260
PDF is available here.
PDF is available here.
Abstract
Abstract
When CdSe/ZnS-polyethyleneimine (PEI) quantum dots (QDs) are microencapsulated in polymeric microcapsules, human fibroblasts are protected from acute cytotoxic effects. Differences in cellular morphology, uptake, and viability were assessed after treatment with either microencapsulated or unencapsula...
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PMID: 21814567
PDF is available here.
PDF is available here.
Abstract
We investigate the feasibility of using MEAs to record from skeletal myotubes derived from primary myoblasts as a way of introducing a third electrogenic cell type and expanding the potential end applications for MEA-based biosensors. We find that the extracellular action potentials (EAPs) produced...
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PMID: 21574266
PDF is available here.
PDF is available here.
Abstract
A comparative H2O2-luminol- and Fe(II)-induced chemiluminescence analysis of extracts of two strains of marine oil oxidizing bacteria Actinetobacter calcoaceticus cultivated either in the presence or absence of oil was carried out. Effects of these extracts on E. coli MG1655 biosensor (pSoxS-lux) wer...
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PMID: 21950119
PDF is available here.
PDF is available here.
Abstract
The contamination, survival, and possible foodborne disease outbreaks are major issues confronting the food industry. However, from a microbial perspective, any food whether natural or processed is just another environmental niche that is available for colonization. Quorum sensing or cell-cell commu...
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PMID: 21031305
PDF is available here.
PDF is available here.
Abstract
We describe two methods for AQ detection and quantification that employ thin layer chromatography (TLC) and microtitre plate assays in combination with a lux-based Pseudomonas aeruginosa AQ biosensor strain. For TLC detection, organic solvent extracts of bacterial cells or spent culture supernatants...
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PMID: 21031301
PDF is available here.
PDF is available here.
Bioassays of quorum sensing compounds using Agrobacterium tumefaciens and Chromobacterium violaceum.
Abstract
We describe several commonly used approaches incorporating C. violaceum and A. tumefaciens that can be used to detect acyl HSLs and QS inhibition....
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PMID: 21031300
PDF is available here.
PDF is available here.
Abstract
We developed an in vitro protein-based assay system for the rapid detection and quantification of the furanosyl borate diester (BAI-2) subclass of autoinducer-2 (AI-2) QS molecules. The biosensor is based on the interaction of BAI-2 with the Vibrio harveyi QS receptor LuxP. Conformation changes asso...
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PMID: 21031302
PDF is available here.
PDF is available here.
Abstract
The detection of bacterial signaling molecules in liquid or gaseous environments has been occurring in nature for billions of years. More recently, man-made materials and systems has also allowed for the detection of small molecules in liquid or gaseous environments. This chapter will outline some e...
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PMID: 21031306
PDF is available here.
PDF is available here.
Abstract
We find useful for Biacore-based studies. Many kinetic-based screening assays require rigorous sample preparation and purification prior to analysis. To highlight these procedures, this protocol describes the kinetic characterisation of single chain Fv (scFv) antibody fragments from crude bacterial...
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PMID: 20978978
PDF is available here.
PDF is available here.
Abstract
Molecular dynamics simulations (90 ns) of different DNA complexes attached to a functionalized substrate in solution were performed in order to clarify the behavior of mismatched DNA sequences captured by a tethered DNA probe (biochip). Examination of the trajectories revealed that the substrate infl...
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PMID: 21660324
PDF is available here.
PDF is available here.
Abstract
We present the method for developing a custom CHO-HCP AMMP assay for bioprocess development. Developing AMMP assays or modifying existing assays into AMMP assays can provide better performance (reproducibility, sensitivity) in complex samples and reduced operator time. The protocol shows the steps f...
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PMID: 21339717
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
Oxygen tension is critical in a number of cell pathways but is often overlooked in cell culture. One reason for this is the difficulty in modulating and assessing oxygen tensions without disturbing the culture conditions. Toward this end, a simple method to generate oxygen-sensitive microwells was d...
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PMID: 20938500
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
We demonstrate for the first time continuous DNA extraction from cell lysate on a microfluidic chip. After mixing bacterial Escherichia coli culture with superparamagnetic bead suspension, lysis and binding buffers, DNA is released from cells and captured by the beads. These DNA carrying beads are c...
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PMID: 20938545
PDF is available here.
PDF is available here.
Lab On a Chip (10)23 2010
Abstract
Stretchable electronics is a revolutionary technology that will potentially create a world of radically different electronic devices and systems that open up an entirely new spectrum of possibilities. This article proposes a microfluidic based solution for stretchable radio frequency (RF) electronic...
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PMID: 20877884
PDF is available here.
PDF is available here.
Cytometry Part A (77)12 2010
Discovering the unknown: detection of emerging pathogens using a label-free light-scattering system.
Abstract
Our study uses a Bayesian approach to learning with a nonexhaustive training dataset to allow for the automated detection of unknown bacterial classes.
© 2010 International Society for Advancement of Cytometry....
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PMID: 21108360
PDF is available here.
PDF is available here.
Acta Biomaterialia (6)12 2010
Abstract
We propose that these materials function by retarding cytokine diffusion, with the non-crosslinked polymers being capable of retarding the diffusion of cytokines in the extracellular matrix and preventing engagement with receptors. In contrast, crosslinked materials have long diffusion lengths into...
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PMID: 20601239
PDF is available here.
PDF is available here.
Abstract
We report for the first time a soluble PQQ-glucose dehydrogenase that is twice more active than the wild type for glucose oxidation and was obtained by combining site directed mutagenesis, modelling and steady-state kinetics. The observed enhancement is attributed to a better interaction between the...
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PMID: 21036156
PDF is available here.
PDF is available here.
Chemical Communications (46)42 2010
Abstract
A promising one-step homogeneous fluoroimmunoassay based on nanoscale-graphene sheets as powerful fluorescence acceptors and CdTe quantum dots as vigorous donors was designed to detect trace biomarker protein with distance-independent quenching efficiency, which significantly broke the distance limi...
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PMID: 20856985
PDF is available here.
PDF is available here.
Abstract
FASEB Journal (24)11 2010
Abstract
We have probed at the single-molecule level the interaction of 1-deoxy-D-xylulose 5-phosphate synthase (DXS), which catalyzes the first step of the MEP pathway, with its two substrates, pyruvate and glyceraldehyde-3-phosphate. The data obtained in this pioneering SMFS analysis of a bisubstrate enzym...
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PMID: 20634351
PDF is available here.
PDF is available here.
Abstract
In this study, a 10-channel up-converting phosphor technology-based lateral flow (TC-UPT-LF) assay was developed to profile antibodies against Yersinia pestis. Ten expressed Y. pestis proteins were covalently conjugated with an up-converting phosphor particle to develop double-antigen sandwich immun...
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PMID: 20801166
PDF is available here.
PDF is available here.
Chemical Communications (46)40 2010
Abstract
Lanthanide-based luminescent probes TPR1 and TPR2 were developed for the detection of hydrogen peroxide (H(2)O(2)) in living systems. The chemoselective reaction of these boronate-protected probes with H(2)O(2) resulted in an enhanced lanthanide sensitization and a 6-fold increase in luminescent int...
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PMID: 20838673
PDF is available here.
PDF is available here.
Chemical Communications (46)40 2010
Abstract
Luminol functionalized gold nanoparticles were used as labels for electrochemiluminescence signal amplification and an ultrasensitive, highly selective, convenient, low cost DNA detection strategy was developed.
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PMID: 20852765
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)11 2010
Abstract
Our model predicts two regimes for biomolecule detection: For classical biosensors with ρ(MP) ≤ ρ(T), the response time t(s) proportional to 1/ρ(T); while for MP-based biosensors with ρ(MP) > ρ(T), t(s) proportional to 1/ρ(MP). The theory (i) explains the performance improvement of MP-sensor...
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PMID: 20820498
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)11 2010
Abstract
We present a review of the current literature concerning the electrochemical applications and advancements of graphene, starting with its use as a sensor substrate through to applications in energy production and storage, depicting the truly remarkable journey of a material that has just come of age...
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PMID: 20890532
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)11 2010
Abstract
This paper describes the use of microarray-based biosensor system for the determination of phenol. Microarrays based on poly(ethylene glycol)(PEG) hydrogel were prepared by photopatterning of a solution containing PEG diacrylate (PEG-DA), photoinitiator, tyrosinase, and CdSe/ZnS quantum dots (QDs)....
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PMID: 20852777
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)11 2010
Abstract
Herein, a sensitive and selective sensor for mercury(II) and glutathione based on the aggregation-induced emission (AIE) of a tetraphenylethene derivative stimulated by Hg(2+)-DNA complexes is reported. Aggregation complexes of AIE probes, quaternized tetraphenylethene salt and anti-Hg(2+) aptamer s...
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PMID: 20877906
PDF is available here.
PDF is available here.
Physiological Measurement (31)11 2010
Abstract
Local pulse-wave velocity (PWV) is an accurate indicator of the degree of arteriosclerosis (stiffness) in an artery, providing a direct characterization of the properties of its wall. Devices currently available for local PWV measurement are mainly based on ultrasound systems and have not yet been g...
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PMID: 20855927
PDF is available here.
PDF is available here.
Abstract
We describe the immobilisation of pyranose-2-oxidase (P2Ox) and the catalytic response to D-glucose on such a nanopatterned surface, which provides optimum access to the active centres of the enzyme....
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PMID: 20835468
PDF is available here.
PDF is available here.
Chemical Communications (46)38 2010
Abstract
A highly sensitive and selective 1,8-naphthyridine-based fluorescent chemodosimeter for Zn(2+) has been designed, synthesized, and applied to the detection of intracellular Zn(2+).
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PMID: 20820508
PDF is available here.
PDF is available here.
Chemical Communications (46)38 2010
Abstract
An alkaline phosphatase activity detection system was constructed based on the different quenching effect of the enzyme substrate and product on the β-CD-functionalized CdTe QDs.
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PMID: 20717601
PDF is available here.
PDF is available here.
Abstract
Lincomycin-treated pumpkin leaves were illuminated with either continuous light or saturating single-turnover xenon flashes to study the dependence of photoinactivation of photosystem II (PSII) on the mode of delivery of light. The flash energy and the time interval between the flashes were varied b...
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PMID: 20643811
PDF is available here.
PDF is available here.
Abstract
MG7-Ag is a kind of gastric cancer-specific tumor-associated antigen and has been investigated to serve as a marker of gastric cancer for early diagnosis.
Surface plasmon resonance (SPR) sensor was used for the detection of MG7-Ag in the sera of gastric cancer patients to develop an innovative, simp...
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PMID: 20887942
PDF is available here.
PDF is available here.
Abstract
We have developed an immunomicroarray for systematic studies of the binding properties of 10 different micro-/nano-sized streptavidin-functionalized beads to a biotin substrate immobilized on SiO(2) with or without surface modification. SiO(2) surface cleaning, immobilized substrate concentration an...
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PMID: 20417734
PDF is available here.
PDF is available here.
Bioresource Technology (101)19 2010
Abstract
For the sensitive determination of Pb(II) from aqueous solutions, a new voltammetric biosensor based on carbon paste electrode modified with Rhizopus arrhizus was developed. The preconcentrated ions at open circuit were reduced by using differential pulse stripping voltammetry techni...
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PMID: 20576569
PDF is available here.
PDF is available here.
Optics Express (18)20 2010
Abstract
We demonstrate label-free optical biosensing using a horizontal air-slotted silicon-rich silicon nitride (SiNx) microdisk resonator. Due to the strong confinement of light in the air-slot, a large resonance shift of 6.2 nm is observed upon reaction of the biotin-functionalized disk with a streptavid...
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PMID: 20940958
PDF is available here.
PDF is available here.
Abstract
We have developed a unique metal nanoparticle (mNPs)-based assay to detect sequence-specific interactions between transcription factor and its corresponding DNA-binding elements. This assay exploits the interparticle-distance dependent optical properties of noble mNPs as sensing element and utilizes...
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PMID: 20704275
PDF is available here.
PDF is available here.
Lab On a Chip (10)18 2010
Abstract
We report an approach utilizing a suspended nanoparticle crystal (S-NPC) as an electrical read-out biosensor based on a nanofluidic electrokinetics principle. As a preliminary demonstration, streptavidin-modified S-NPC with a particle diameter of 520 nm was used to detect biotin in a PBS buffer. The...
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PMID: 20544114
PDF is available here.
PDF is available here.
Chemico-Biological Interactions (187)1-3 2010
Abstract
This paper reviews our previously published data and presents new results on biosensor assay of blood esterases. Tyrosinase and choline oxidase biosensors based on nanostructured polyelectrolyte films were developed for these purposes. Experiments were performed on the quantitative determination of...
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PMID: 20097186
PDF is available here.
PDF is available here.
Abstract
Metal nanowire patterning in the form of grating structures has been carried out using a wide range of lithography techniques, and many hybrid methods derived from them. The challenge is to achieve sub-100 nm linewidths with controllable spacing and thickness over large areas of substrates with high...
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PMID: 20945550
PDF is available here.
PDF is available here.
Nanotechnology (21)35 2010
Abstract
We demonstrated enhanced localized surface plasmon resonance (SPR) biosensing based on subwavelength gold nanoarrays built on a thin gold film. Arrays of nanogratings (1D) and nanoholes (2D) with a period of 200 nm were fabricated by electron-beam lithography and used for the detection of avian infl...
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PMID: 20693616
PDF is available here.
PDF is available here.
Abstract
We compared the efficiencies of biosensors that use gold-coated single-crystal silicon and amorphous glass substrates. The reflectivity of light over a wide range of wavelengths was higher from gold layer coated single-crystal silicon substrates than from glass substrates. Furthermore, the efficienc...
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PMID: 20628872
PDF is available here.
PDF is available here.
Abstract
We aimed to develop molecular imaging procedures for faster, earlier, and easier recognition of aortic aneurysms. We show that in vivo coregistration of MMP activity by noninvasive tomographic imaging methods allows the detection of increased MMP activity, even before the aneurysm has actually forme...
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PMID: 20592247
PDF is available here.
PDF is available here.