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Epitope Mapping (0):
Recent article
Epitope Mapping
Abstract
We isolated a murine monoclonal antibody (mAb) against A13 from a mouse that had been infected with VACV. The anti-A13 mAb bound to recombinant A13 protein with an affinity of 3.4nM and neutralized VACV mature virions. Passive immunization of mice with the anti-A13 mAb protected against intranasal V...
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PMID: 21810533
PDF is available here.
PDF is available here.
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New England Journal of Medicine (364)22 2011
Abstract
We searched for antibodies against bovine serum albumin and circulating bovine serum albumin by means of enzyme-linked immunosorbent assay and Western blotting in serum specimens obtained from 50 patients with membranous nephropathy and 172 controls. The properties of immunopurified circulating bovi...
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PMID: 21631322
PDF is available here.
PDF is available here.
Abstract
Open reading frame 2 (ORF2) of porcine circovirus type 2 (PCV2) codes for the 233 amino acid capsid protein (CP). Baculovirus-based vaccines that express only ORF2 are protective against clinical disease following experimental challenge or natural infection. The goal of this study wa...
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PMID: 21430122
PDF is available here.
PDF is available here.
Abstract
Although pecans are associated with IgE-mediated food allergies, the allergens responsible remain to be identified and characterized. The 2S albumin gene was amplified from the pecan cDNA library. Dot-blots were used to screen the recombinant protein with pecan allergic patients' serum. The affinity...
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PMID: 21395309
PDF is available here.
PDF is available here.
Abstract
Three pairs of specific primers were designed to amplify F2-1, F2-2, and XF2-2 truncated capsid protein genes of porcine circovirus type 2 (PCV-2). Amplified sequences were subcloned to pET-32a(+) vectors and expressed in Rosetta (DE3) Escherichia coli by induction of isopropy-β-D-thiogalactoside (I...
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PMID: 21461197
PDF is available here.
PDF is available here.
Abstract
We describe a novel series of 10 antibodies towards GPA, recognizing four extra- and intracellular peptide epitopes of this molecule (defined by epitope mapping) and one mixed peptide/carbohydrate epitope. All antibodies bind better to the desialylated than to the fully sialylated molecule, includin...
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PMID: 20727998
PDF is available here.
PDF is available here.
Abstract
We have highlighted their use in celiac disease (CD). The available techniques, which include two-dimensional (2D) gel electrophoresis, mass spectrometry, and antibody and tissue arrays, have been used to identify proteins or changes in protein expression specific to gut tissue from patients with CD...
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PMID: 20632103
PDF is available here.
PDF is available here.
Nature Biotechnology (28)11 2010
Abstract
We apply the latter strategy to antibody discovery, using a library of ∼10,000 human germline antibody Fabs created by de novo DNA synthesis and automated protein expression and purification. In multiplexed screening assays, we obtained specific hits against seven of nine antigens. Using sequence-...
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PMID: 20972421
PDF is available here.
PDF is available here.
Abstract
The hallmark of adaptive immunity is its ability to recognise a wide range of antigens and technologies that capture this diversity are therefore of substantial interest. New methods have recently been developed that allow the parallel analysis of T cell reactivity against vast numbe...
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PMID: 20177676
PDF is available here.
PDF is available here.
Immunotherapy (2)5 2010
Abstract
This meeting was dedicated to various autoimmune diseases and their mechanisms, diagnosis and therapies. The autoimmunity-promoting factors included genetic variations and environmental injuries. A broad array of cytokines, including the B-cell activating factor, and autoantibodies,...
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PMID: 20874644
PDF is available here.
PDF is available here.
Abstract
Newcastle disease (ND) caused by virulent Newcastle disease virus (NDV) is a highly contagious viral disease of poultry. Virulent NDVs characteristically have a multibasic amino acid sequence (virulence motif) such as (112)RRQKRF(117) at the cleavage site of the precusor fusion (F0) protein. The ant...
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PMID: 20706027
PDF is available here.
PDF is available here.
Abstract
We have generated a panel of novel monoclonal antibodies (MAbs) against VLY. For the generation of MAbs, we have used recombinant VLY expressed in Escherichia coli. The functional activity of recombinant VLY was confirmed by an in vitro hemolytic assay using human erythrocytes. The MAbs raised again...
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PMID: 20298711
PDF is available here.
PDF is available here.
Virus Research (151)2 2010
Abstract
We identified a continuous epitope (55)WIRNNF(60) that was recognized by MAb N2C3. Subsequent studies found that this MAb could recognize several different strains of Noroviruses, not just GII/4. In the present study, we used homology modeling to confirm that the epitope of N2C3 was exposed on the s...
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PMID: 20417671
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
The spread of the recently emerged, highly pathogenic H5N1 avian influenza virus has raised concern. Preclinical studies suggest that passive immunotherapy could be a new form of treatment for H5N1 virus infection. Here, a neutralizing monoclonal antibody (MAb) against the hemagglutinin (HA) of the...
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PMID: 20519402
PDF is available here.
PDF is available here.
Abstract
The development of Goodpasture's disease may be considered an autoimmune "conformeropathy" that involves perturbation of the quaternary structure of the alpha345NC1 hexamer, inducing a pathogenic conformational change in the alpha3NC1 and alpha5NC1 subunits, which in turn elicits an autoimmune respo...
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PMID: 20660402
PDF is available here.
PDF is available here.
Advances in Therapy (27)7 2010
Abstract
We identified several high-affinity fully human monoclonal antibodies with inhibitory activity against both human and rodent IGF.1Rs.
These candidate therapeutic antibodies recognized several distinct epitopes and effectively blocked ligand-mediated receptor signal transduction and cellular prolifer...
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PMID: 20574692
PDF is available here.
PDF is available here.
Abstract
We hope to provide an overview of the techniques used in the construction of these tetramers, the problems and solutions associated with them, and the methods used in the study of antigen-specific T cells. Understanding how the antigen-specific cells develop and function in different circumstances a...
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PMID: 20624049
PDF is available here.
PDF is available here.
Abstract
HLA-A 0201-restricted virus-specific CD8(+) CTL do not appear to control HIV effectively in vivo. To enhance the immunogenicity of a highly conserved subdominant epitope, TV9 (TLNAWVKVV, p24 Gag(19-27)), mimotopes were designed by screening a large combinatorial nonapeptide library w...
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PMID: 20432235
PDF is available here.
PDF is available here.
Abstract
We confirmed that the initial DDDAM regimen, which mimics one of the clinical schedules (DDDCM), is highly immunogenic in macaques. Furthermore, adjuvanted synthetic long peptides divided into sub-pools and delivered into anatomically separate sites induced T-cell responses that were markedly broade...
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PMID: 20468055
PDF is available here.
PDF is available here.
Abstract
We previously constructed a humanized antibody, HuS10, by grafting the complementarity-determining regions (CDRs) of a parental murine monoclonal antibody into the homologous human antibody sequences. This process is termed CDR grafting. Some residues that were thought to affect the CDR loops and st...
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PMID: 20399745
PDF is available here.
PDF is available here.
Clinica Chimica Acta (411)9-10 2010
Abstract
The antibody used for this ELISA had the same cross-reactivity with MT in humans and experimental animals. NH2 terminal peptide of MT with acetylated methionine was proved to be the epitope of this antibody. The reactivity of this ELISA system with liver, kidney and brain in MT1/2 knock-out mice was...
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PMID: 20184868
PDF is available here.
PDF is available here.
Microbes and Infection (12)5 2010
Abstract
We identified a HLA-A*0201-restricted epitope, LigA(305-313) KLIVTPAAL in Leptospira LigA. CTLs specific for LigA(305-313) were elicited both in HLA-A2.1/K(b) transgenic mice and in patients with a clinical and/or laboratory diagnosis of leptospirosis. Staining of the HLA-A*0201-LigA(305-313) tetram...
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PMID: 20123035
PDF is available here.
PDF is available here.
Abstract
The Antibody Engineering & Design 2010 conference, held in Frankfurt, included topics covering new developments in the field of therapeutic antibodies. This conference report highlights selected presentations on 'fit for purpose' biologics, improving the pharmaceutical properties of antibodies, the...
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PMID: 20432182
PDF is available here.
PDF is available here.
Veterinary Pathology (47)3 2010
Abstract
Eyes and nuclei of the visual pathways in the brain were examined in 30 Rocky Mountain elk (Cervus elaphus nelsoni) representing 3 genotypes of the prion protein gene PRNP (codon 132: MM, ML, or LL). Tissues were examined for the presence of the abnormal isoform of the prion protein associated with...
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PMID: 20382822
PDF is available here.
PDF is available here.
Anticancer Research (30)5 2010
Abstract
A monoclonal antibody against the potential tumor suppressor kinase-enhanced protein phosphatase 1 (PP1) inhibitor KEPI (PPP1R14C) was generated and characterized. Human KEPI was expressed in Escherichia coli and used to immunize Balb/c mice. Using hybridoma technology, one clone, G18AF8, was isolat...
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PMID: 20592344
PDF is available here.
PDF is available here.
Abstract
Two genotypes of porcine circovirus (PCV) have been described, the non-pathogenic PCV1 and the pathogenic PCV2 in pigs. PCV-ORF1 encodes Rep and Rep' proteins which have identical N-terminal sequence (RepN) within each PCV strain, but RepN has only 88% similarity between PCV1 and PCV2. Purified RepN...
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PMID: 20152863
PDF is available here.
PDF is available here.
Abstract
We identified two overlapping neutralizing epitopes within residues 151 to 172 of the central unglycosylated region of the respiratory syncytial virus (RSV) attachment protein. In approximately 40% of hospitalized and outpatient adults infected with RSV subtype A, these contiguous residues are the t...
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PMID: 20164253
PDF is available here.
PDF is available here.
Abstract
I HLA. These results reveal that of all RD proteins, a significant number of these peptides are predicted to be high-affinity HLA binders (T (1/2) >or= 100 min), irrespective of the length of the protein, and 67% of the peptides predicted to bind are mono-allelic in their binding. Pathogen peptides...
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PMID: 19609720
PDF is available here.
PDF is available here.
Abstract
The autoimmune response to PSTI protein may induce a failure of PSTI activity, resulting in the activation of trypsinogen and the subsequent disease progression....
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PMID: 19812526
PDF is available here.
PDF is available here.
Abstract
CD132 is the common gamma chain to a number of cytokine receptor complexes including that for IL-2. To identify the functional domain of chicken CD132 (chCD132), the cDNA of chCD132 was cloned, and a neutralizing monoclonal antibody, raised against a recombinant chCD132 protein, was identified by in...
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PMID: 19819255
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)3 2010
Abstract
The antigenic epitopes of 2-G1 mAb were localized within amino acids (aa) 76-90 and 109-123 in APE1 redox domain, belonging to the conformational epitope category. The antigenic epitope of 4-F6 mAb was within aa 109-147 in DNA repair endonuclease domain. In the range of 8.0 microg/L to 200 microg/L,...
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PMID: 20230692
PDF is available here.
PDF is available here.
Abstract
The Crimean-congo hemorrhagic fever virus (CCHFV) is a geographically widespread fatal pathogen. Identification of the epitope regions of the virus is important for the diagnosis and epidemiological studies of CCHFV infections. In this study, expression vectors carrying series truncated fragments of...
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PMID: 20960283
PDF is available here.
PDF is available here.
Human Immunology (71)2 2010
Abstract
We have characterized 25 antigens as a vaccine candidate and screened the potential T-lymphocyte epitopes presented by human leukocyte antigen (HLA)-A, -B, and -DR molecules based on the proteomic data of Plasmodium falciparum. Of the 25 proteins, 22 were predicted as probable antigens and two were...
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PMID: 19945493
PDF is available here.
PDF is available here.
Abstract
We mapped the major immunodeterminants of the E. chaffeensis 200-kDa ankyrin protein (Ank200) and the minor immunodeterminants in the N- and C-terminal regions of E. chaffeensis TRP47. Major antibody epitopes of Ank200 were localized to four polypeptide regions (18-mer, 20-mer, 20-mer, and 21-mer, r...
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PMID: 19955322
PDF is available here.
PDF is available here.
Thrombosis and Haemostasis (103)1 2010
Abstract
We characterised, for the first time, a unique anti-FVIII monoclonal antibody, mAb216, that enhanced FVIII coagulant activity. The mAb216 shortened the activated partial thromboplastin time and specifically increased FVIII activity by approximately 1.5-fold dose-dependently. FXa generation and throm...
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PMID: 20062922
PDF is available here.
PDF is available here.
Abstract
We used a DiscoTope 1.2 (http://www.cbs.dtu.dk/services/DiscoTope/), Epitopia (http://epitopia.tau.ac.il/) and EPCES (http://www.t38.physik.tu-muenchen.de/programs.htm) algorithms to map discontinuous B-cell epitopes in HIV1 gp120. The most mutable nucleotides in HIV genes are guanine (because of G...
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PMID: 20653424
PDF is available here.
PDF is available here.
Abstract
We analysed MSI-High colorectal cancer cell lines for examples of naturally expressed mutant proteins arising from frameshift mutations in coding microsatellites (cMS) by immunoprecipitation and Western Blot experiments. Detected mutant protein bands from NMD-resistant transcripts were further valid...
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PMID: 21209843
PDF is available here.
PDF is available here.
BMC Structural Biology (10 Suppl 1) 2010
Abstract
The proposed method leads to a new way of identifying B-cell epitopes. Besides, this antibody-specified epitope prediction can provide more precise and helpful information for wet-lab experiments....
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PMID: 20487513
PDF is available here.
PDF is available here.
Abstract
We present SAROTUP, a free web tool for scanning, reporting and excluding possible target-unrelated peptides from real mimotopes. Preliminary tests show that SAROTUP is efficient and capable of improving the accuracy of mimotope-based epitope mapping. It is also helpful for the development of mimoto...
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PMID: 20339521
PDF is available here.
PDF is available here.
Abstract
We have established "IMMUNOCAT", an interactive data management system for the epitope discovery research projects conducted by our group. The system provides functions to store, query, and analyze clinical and experimental data, enabling efficient, systematic, and integrative data management. We de...
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PMID: 20490281
PDF is available here.
PDF is available here.
Abstract
Two immunodominant T-cell-reactive regions of Pru p 3 were identified: Pru p 3(12-27) and Pru p 3(57-72). These peptides showed a differential ability to elicit a Th2 response. Taken together, our results provide a better understanding of the immunological T-cell reactivity against Pru p 3.
Copyrigh...
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PMID: 20357479
PDF is available here.
PDF is available here.
Abstract
We have been able to visualize conformational epitopes on allergens.
Copyright 2009 S. Karger AG, Basel....
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PMID: 19940500
PDF is available here.
PDF is available here.
Abstract
PBMCs from 12 of 35 subjects responded to M. tuberculosis-specific antigens ESAT-6 and CFP10 as well as to MPT64, which suggested that they were infected with M. tuberculosis. Ten of twelve T-cell lines established from these donors responded to MPT64, and nine T-cell lines responded to 1 or more of...
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PMID: 20134175
PDF is available here.
PDF is available here.
Abstract
Long-term SCIT (>1 year; cumulative dose >1,000,000 SQ units) induced more pronounced IgG1, IgG2 and IgG4 responses to Bet v 1 and Bet v 1-related allergens according to the degree of sequence homology (Bet v 1>Aln g 1>Cor a 1>Mal d 1) than short-term SCIT (<1 year; cumulative dose <1,000,000 SQ uni...
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PMID: 19672093
PDF is available here.
PDF is available here.
Abstract
Immunogenicity is a major limitation to therapy with certain monoclonal antibodies and proteins. A major driver for immunogenicity is the presence of human T-cell epitopes within the protein sequence which can activate helper T-cells resulting in the sustained production of antibodies and neutraliza...
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PMID: 19252848
PDF is available here.
PDF is available here.
Abstract
DNA Abeta42 trimer immunization resulted in antibody titers with a mean of 15 microg per milliliter of plasma. The isotype profile of the antibodies differed markedly. A predominant IgG1 antibody response was found in the DNA-immunized mice, indicating a T(H)2 type of immune response (IgG1/IgG2a rat...
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PMID: 19861672
PDF is available here.
PDF is available here.
Abstract
The genome of rice dwarf phytoreovirus (RDV) is composed of 12 double-stranded RNA segments, of which segment S6 encodes a non-structural protein Pns6 identified as the movement protein. In this report, Pns6 with a 6-histidine tag at the N-terminal was expressed in E. coli after induction under low...
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PMID: 19911132
PDF is available here.
PDF is available here.
Abstract
A series of DNA vaccines based on the bcr-abl fusion gene were developed and tested in mice. Two mouse (BALB/c) bcr-abl-transformed cell lines, B210 and 12B1, which both expressed p210bcr-abl and were oncogenic for syngeneic animals but differed in some other respects, were used as a model system. I...
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PMID: 19724931
PDF is available here.
PDF is available here.
Abstract
We developed a new experimental model for studying the mechanism of formalin fixation and antigen retrieval. Epitope mapping studies on nine antibodies useful for FFPE tissues revealed that each consisted of a contiguous stretch of amino acids in the native protein (linear epitope). Small peptides r...
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PMID: 19886757
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)37 2009
Abstract
We report the cloning and functional characterization of this Ae. aegypti Cry11Aa-ALP receptor. Of three ALP's cDNA clones, the recombinant produced ALP1 isoform was shown to bind Cry11Aa and P1.BBMV peptide phage that specifically binds the midgut ALP-Cry11Aa receptor. An anti-ALP1 antibody inhibit...
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PMID: 19697959
PDF is available here.
PDF is available here.
Analytical Biochemistry (396)1 2009
Abstract
Monoclonal antibodies (mAbs) and antibody fragments have become an emerging class of therapeutics since 1986. Their versatility enables them to be engineered for optimal efficiency and decreased immunogenicity, and the path to market has been set by recent regulatory approvals. One o...
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PMID: 19720041
PDF is available here.
PDF is available here.
Abstract
We discuss in this Review, the arrival of the genome era has revolutionized vaccine development and catalyzed a shift from conventional culture-based approaches to genome-based vaccinology. The availability of complete bacterial genomes has led to the development and application of high-throughput a...
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PMID: 19729849
PDF is available here.
PDF is available here.
Abstract
Our results show that epitopes identified by the peptide microarray were mostly consistent with those identified previously by SPOT membrane technology, but with specific binding to a few newly identified epitopes of milk allergens. Data from replicate arrays were reproducible (r > or = 0.92) regard...
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PMID: 19577281
PDF is available here.
PDF is available here.
Abstract
Combinatorial phage display libraries of random peptides can be used to discover the epitopes of antibodies through a procedure termed "biopanning." The affinity isolation of phage-displayed epitope peptidomimetics allows molecular definition of the epitopes of monoclonal antibodies (MAbs). Panels o...
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PMID: 19653209
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)30 2009
Abstract
We generated a fully human high-affinity antibody against FasL that binds to and neutralizes the activity of both soluble and membrane-associated human FasL. In order to elucidate the mechanism of function of this antibody, we have mapped the region and critical residues involved in the recognition...
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PMID: 19588926
PDF is available here.
PDF is available here.
Abstract
Antibodies against MICA antigens have been determined either using cells transfected with MICA alleles or recombinant MICA antigens. MICA epitopes were characterized by empirical study of human sera and by correlation with MICA polymorphic amino acids. Sera were absorbed with cells transfected with...
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PMID: 19610173
PDF is available here.
PDF is available here.
Abstract
HLAMatchmaker is a computer algorithm that considers small configurations of polymorphic residues referred to as eplets as essential components of HLA epitopes. This overview describes recent developments that have increased our understanding of structural epitope antigenicity, that is, reactivity w...
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PMID: 19417654
PDF is available here.
PDF is available here.
Journal of Autoimmunity (33)1 2009
Abstract
Approximately 1% of hybridomas utilizing different TCR beta chains paired with the conserved TRAV5D-4*04 containing alpha chains respond to insulin peptide B:9-23. Therefore, TCR beta chain sequences make an important contribution to insulin B:9-23 peptide recognition but multiple beta chain sequenc...
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PMID: 19286348
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (284)31 2009
Abstract
We applied a novel approach to select monoclonal phosphospecific antibodies directly from the full-length protein and without up-front phosphoamino acid identification. We chose as antigen GRASP65, a well studied Golgi phosphoprotein. Bacterially produced full-length protein was first incubated with...
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PMID: 19473967
PDF is available here.
PDF is available here.
Abstract
We separated CD34 into 2 glycoforms, the L-selectin-binding and nonbinding glycoforms, L-B-CD34 and L-NB-CD34, respectively, and analyzed released O- and N-glycans from both forms. L-B-CD34 is relatively minor compared with L-NB-CD34 and represented less than 10% of total tonsillar CD34. MECA-79, a...
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PMID: 19359410
PDF is available here.
PDF is available here.