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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Protein Interaction Mapping (3):
Recent article
Protein Interaction Mapping
Journal of General Virology (91)Pt 8 2010
Abstract
We observed that UL114 was much less abundant than UL44. Interaction of UL114 with UL54, independent of the UL54 carboxyl terminus, but not with UL44 was detected in vitro. Our data are consistent with a direct UL114-UL54 interaction, and suggest that UL114 and UL54 act in concert during base excisi...
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PMID: 20410316
PDF is available here.
PDF is available here.
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Journal of General Virology (91)Pt 8 2010
Abstract
We conducted a yeast two-hybrid screening. As a result, we identified a cellular protein termed bovine NIK- and IKKbeta-binding protein (NIBP), which is involved in protein trafficking and nuclear factor kappa B (NF-kappaB) signalling in cells. The interaction of NS5A with NIBP was confirmed both in...
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PMID: 20444997
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We showed that SUMO binding by IE2 is necessary for its efficient transactivation function and for viral growth. IE2 bound physically to SUMO-1 through a SUMO-interacting motif (SIM). Mutations in SIM (mSIM) or in both SUMOylation sites and SIM (KR/mSIM), significantly reduced IE2 transactivation ef...
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PMID: 20519406
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We show here that another Nedd4-like ubiquitin ligase, Nedd4-1, corrected release defects resulting from the disruption of PTAP (PTAP(-)), suggesting that HIV-1 Gag also recruits Nedd4-1 to facilitate virus release. Notably, Nedd4-1 remediation of HIV-1 PTAP(-) budding defects is independent of cell...
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PMID: 20519395
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
Retroviral integrases associate during the early viral life cycle with preintegration complexes that catalyze the integration of reverse-transcribed viral cDNA into the host chromosomes. Several cellular and viral proteins have been reported to be incorporated in the preintegration complex. This stu...
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PMID: 20519390
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We investigated the role of an SH3 domain binding site centered on a PPLP motif in K15. We screened libraries of cellular SH3 domains to identify signaling molecules interacting with the KSHV PPLP motif. We found its affinities for two Src kinase family members, Lyn and Hck, to exceed those of other...
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PMID: 20534855
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We report that EV71 suppresses the induction of antiviral immunity by targeting the cytosolic receptor retinoid acid-inducible gene I (RIG-I). In infected cells, EV71 inhibits the expression of IFN-beta, IFN-stimulated gene 54 (ISG54), ISG56, and tumor necrosis factor alpha. Among structural and non...
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PMID: 20519382
PDF is available here.
PDF is available here.
Virus Research (151)2 2010
Abstract
We performed a yeast-2-hybrid screen to identify cellular proteins that interact with EIAV S2. The screen identified two human cellular proteins, amplified in osteosarcoma (OS-9) and proteasome 26S ATPase subunit 3 (PSMC3) that interact with S2. The equine homologues of these proteins were cloned an...
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PMID: 20417672
PDF is available here.
PDF is available here.
Virus Research (151)2 2010
Abstract
Hantaviral N and Gn proteins were shown to interact, thus providing the long-awaited evidence for one of the crucial steps in the virus replication at which RNPs are directed to the site of the virus assembly. Using pull-down assay and point mutagenesis it was demonstrated that intact, properly fold...
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PMID: 20566401
PDF is available here.
PDF is available here.
Biology of the Cell (102)8 2010
Abstract
We have determined the intracellular localization of the protein components of the 42S particle by expressing fluorescent protein-tagged fusions in transparent previtellogenic oocytes. 42Sp50 and its isoforms (EF1A-S and EF1A-O) were excluded from the nuclei and distributed uniformly throughout the...
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PMID: 20415665
PDF is available here.
PDF is available here.
Abstract
We attempted to identify profiles of proteins with altered expression levels in rats following unilateral stereotaxic injection of 6-hydroxydopamine into the SNc. Protein expression profiles of these proteins in the substantia nigra and the striatum were made using two-dimensional gel electrophoresi...
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PMID: 20403401
PDF is available here.
PDF is available here.
Trends in Biotechnology (28)8 2010
Abstract
We discuss how new methodological strategies derived from non-invasive fluorescence- and luminescence-based approaches (FRET, BRET, BiFC and BiLC), when applied to the study of G protein-coupled receptor (GPCR) oligomerization, can be used to detect specific PPIs in live cells. These technologies al...
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PMID: 20542584
PDF is available here.
PDF is available here.
Abstract
The peroxisome proliferator-activated receptor-gamma (PPAR-gamma) coactivator-1 beta (PGC-1 beta) is a well-established regulator of the beta-oxidation of fatty acids and the oxidative phosphorylation in mitochondria. However, the underlying mechanism of PGC-1 beta action remains elusive. This study...
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PMID: 20561910
PDF is available here.
PDF is available here.
Abstract
Specific protein-protein interaction is essential for the function of life systems. A variety of computational methods are being extensively used now-a-days to investigate this interaction and to identify structural features of binding sites. In this paper, the informational structure analysis metho...
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PMID: 20476797
PDF is available here.
PDF is available here.
Abstract
We identified several highly connected proteins: 14-3-3 Zeta, 14-3-3 epsilon, actin, aldolase A, calmodulin, cofilin 1, cystatin C, fatty acid-binding protein 4, profilin 1, prolyl 4-hydrolase, peptidylprolyl isomerase, and transgelin. This study provides the first detailed description of protein ch...
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PMID: 20160674
PDF is available here.
PDF is available here.
Abstract
CD (circular dichroism) spectroscopy is a well-established technique in structural biology. SRCD (synchrotron radiation circular dichroism) spectroscopy extends the utility and applications of conventional CD spectroscopy (using laboratory-based instruments) because the high flux of a synchrotron en...
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PMID: 20658968
PDF is available here.
PDF is available here.
Abstract
We commonly accept that many, if not all, enzymes act in close association with others. Co-precipitation using an antibody against a test protein is the standard assay for the identification of members of protein complexes [Musso, Zhang and Emili (2007) Chem. Rev. 107, 3585-3600]. The introduction o...
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PMID: 20658978
PDF is available here.
PDF is available here.
Abstract
We describe the two standard high-throughput methods for identification of protein complexes: two-hybrid screens and TAP (tandem affinity purification) tagging. These methods have been used to characterize the interactome of Saccharomyces cerevisiae, showing that the majority of proteins are part of...
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PMID: 20658969
PDF is available here.
PDF is available here.
Abstract
We really able to understand about complex formation and can any of this information provide a useful tool to aid drug development? Correlations between thermodynamic data and structural detail have been investigated, allowing insight into ways in which these can be used to understand protein-ligand...
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PMID: 20658972
PDF is available here.
PDF is available here.
Abstract
We review some of the advances that have been made in the two different types of sedimentation experiment--sedimentation equilibrium and sedimentation velocity--for the analysis of protein-protein interactions and indicate how major complications such as thermodynamic and hydrodynamic non-ideality c...
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PMID: 20658974
PDF is available here.
PDF is available here.
Abstract
We review our current understanding of their geometry and evolution, including aspects of the symmetry of these complexes and their interaction interfaces. Also, we briefly discuss the pathway of their assembly in solution....
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PMID: 20658970
PDF is available here.
PDF is available here.
Abstract
Most cellular processes are carried out by a multitude of proteins that assemble into multimeric complexes. Thus a precise understanding of the biological pathways that control cellular events relies on the identification and on the biochemical characterization of the proteins involved in such multi...
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PMID: 20658971
PDF is available here.
PDF is available here.
Abstract
We discuss some of our recent research in the development of a scalable assay to identify this class of protein interaction and some of the findings from its application in the construction of extracellular protein interaction networks....
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PMID: 20658977
PDF is available here.
PDF is available here.
Abstract
We show that CRP unexpectedly binds to CFH at two non-contiguous sites and explain its relevance to age-related macular degeneration....
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PMID: 20658973
PDF is available here.
PDF is available here.
Abstract
I band (approx. 45 cm(-1) towards lower frequency) upon (13)C labelling of one of the proteins reveals the amide I band of the unlabelled protein, enabling it to be used as a probe for monitoring conformational changes. With site-specific isotopic labelling, structural resolution at the level of ind...
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PMID: 20658981
PDF is available here.
PDF is available here.