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Molecular Probe Techniques (9):
Recent article
Molecular Probe Techniques
Proteomics (11)4 2011
Abstract
We review several emerging targeted technologies to chart both N-terminal acetylation as well as acetylation at the lysine side chain, on a proteome-wide scale, highlighting in particular studies that have expanded the biological knowledge on the appearance and function of these common but functiona...
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PMID: 21246731
PDF is available here.
PDF is available here.
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Abstract
We implemented a strategy for the visualization of morphological changes of dendritic spines over tens of minutes at a lateral resolution of 25 to 65 nm. We have generated a low-affinity photoconvertible probe, capable of reversibly binding to actin and thus allowing long-term photoactivated localiz...
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PMID: 21264214
PDF is available here.
PDF is available here.
Abstract
Quantum dots (QDs) are new generation of fluorophores with superior optical properties. For biological applications of QDs, proper surface modification and further conjugation with biomolecules are necessary to make these nanocrystals biocompatible as well as target-recognizable. Preparation of QDs...
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PMID: 20497111
PDF is available here.
PDF is available here.
Abstract
Recent clinical practice for the management for cancer patients has begun to change from a statistical "one-size fits all" approach to medicine to more individualized care. Pre-treatment biomarkers (i.e. genetically and histologically based) have a growing role in providing guidance related to the a...
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PMID: 20504274
PDF is available here.
PDF is available here.
Abstract
Fluorescence-based molecular imaging in small animals is having a major impact on drug development and disease research and the ability to detect multiple molecular species at once is becoming increasingly important. Unlike bioluminescence, in fluorescence, ubiquitous autofluorescent signals from th...
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PMID: 20497114
PDF is available here.
PDF is available here.
Abstract
Hyperpolarized (HP) (13)C labeled compounds can be used as MR contrast agents to investigate metabolic pathways in vivo in almost real time. To date, a high proportion of reported studies have utilized HP 1-(13)C pyruvate to investigate intracellular metabolism in tumors and other tissues. The long...
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PMID: 20497107
PDF is available here.
PDF is available here.
Abstract
Molecular imaging has become a research focus in recent years, which provides an effective information acquisition, analysis and processing methodology at cellular and molecular levels for biomedical study. As an important molecular imaging technique, optical molecular imaging, especially fluorescen...
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PMID: 20497115
PDF is available here.
PDF is available here.
Abstract
The prospect of mobilizing the power of the immune system in the fight against cancer has fascinated generations of immunologists. After decades of sporadic successes scattered amongst frequent failures, the field of tumor immunology is now poised to take advantage of a greatly improved understandin...
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PMID: 20497110
PDF is available here.
PDF is available here.
Abstract
We will introduce the current state of development of dual-targeted molecular imaging probes and summarize the various dual-targeted molecular probes for in vivo cancer imaging....
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PMID: 20497116
PDF is available here.
PDF is available here.
Abstract
We have previously shown that channels formed by the linear gramicidins are suitable probes for changes in lipid bilayer properties, as experienced by bilayer-spanning proteins. We now report a gramicidin-based fluorescence assay for changes in bilayer properties. The assay is based on measuring the...
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PMID: 20233091
PDF is available here.
PDF is available here.
Abstract
Noninvasive cardiovascular imaging techniques are well-established for studying cardiovascular anatomy and physiology. Over the past decade contrast enhanced imaging techniques have been developed that are also able to characterize the molecular constituents of cardiovascular disease. In this regard...
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PMID: 20467852
PDF is available here.
PDF is available here.
Abstract
A water-soluble, biodegradable and fluorescent hyperbranched poly(amidoamine) with mannose groups on their surface (M-HPAMAM) has been successfully prepared, and the synthetic strategy includes Michael addition polymerization of diacrylamide with 1-(2-aminoethyl)piperazine and, subsequently, surface...
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PMID: 20527872
PDF is available here.
PDF is available here.
Lab On a Chip (10)11 2010
Abstract
We will review the implementation of various microfluidic assays which employ FRET for diverse applications in the biomedical field, along with the advantages and disadvantages of the various approaches. The future prospects for development of microfluidic devices incorporating FRET detection will b...
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PMID: 20480105
PDF is available here.
PDF is available here.
Der Internist (51)6 2010
Abstract
Molecular imaging uses the molecular signature of cells for targeted minimally-invasive detection and characterization of gastrointestinal pathologies. Exogenous fluorescent agents serve as molecular beacons for visualization of specific surface markers or metabolic activity in the target tissue. Mo...
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PMID: 20424814
PDF is available here.
PDF is available here.
Abstract
Genetics in Medicine (12)6 2010
Abstract
We identified four unbalanced rearrangements: one del 18pter/amp 5pter, one del 9pter, one 15q11q13 microdeletion, and one 22q11 microdeletion with atypical presentation. After genetic counseling, two of the pregnancies were terminated. CONCLUSION: Multiplex ligation probe amplification analysis was...
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PMID: 20453657
PDF is available here.
PDF is available here.
Nanotechnology (21)17 2010
Abstract
We report the synthesis and characterization of a dual-modality nanoprobe that couples the magnetic properties of ultrasmall superparamagnetic iron oxide nanoparticles (USPIOs) with the near infrared fluorescence of Cy5.5. The fluorophore is encapsulated in a biocompatible shell of silica surroundin...
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PMID: 20368682
PDF is available here.
PDF is available here.
Abstract
Overhauser-enhanced magnetic resonance imaging (OMRI) studies of a membrane-permeable nitroxyl spin probe, (2)H-enriched 3-methoxycarbonyl-2,2,5,5-tetramethyl-pyrrolidine-1-oxyl (MC-PROXYL), used in simultaneous molecular imaging is reported. Phantom imaging was performed with liposomal solutions of...
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PMID: 20226702
PDF is available here.
PDF is available here.
Abstract
A novel strategy for the development of molecular aptamer beacon for a signal transduction protein, myotonic dystrophy kinase-related Cdc42-binding kinase (MRCK) was proposed in this work. MRCK is an important downstream effector protein of Cdc42 that phosphorylates proteins involved in organizing a...
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PMID: 20188990
PDF is available here.
PDF is available here.
Avian Pathology (39)2 2010
Abstract
We report the development and validation of a duplex real-time polymerase chain reaction (PCR) assay with an internal control using TaqMan-labelled probes for the detection of Mycoplasma gallisepticum and Mycoplasma synoviae (duplex MGMS PCR). The MGMS PCR was highly specific with a sensitivity of 7...
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PMID: 20390544
PDF is available here.
PDF is available here.
Der Radiologe (50)4 2010
Abstract
Optics Letters (35)7 2010
Imaging of molecular probe activity with Born-normalized fluorescence optical projection tomography.
Abstract
We demonstrate its capability to tomographically visualize molecular activity in whole organs of mice. In particular, eosinophil activity in asthmatic lungs is resolved using a Born-normalized fluorescence optical projection tomography and employing a near-IR molecular probe. The possibility to achi...
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PMID: 20364226
PDF is available here.
PDF is available here.
Abstract
Atherosclerotic plaque disruption accounts for the major part of cardiovascular mortality and the risk of disruption appears to depend on plaque composition. Carotid plaques in patients, scheduled for endarterectomy, have been successfully characterised with MRI. MRI has the advantage of combining i...
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PMID: 20112134
PDF is available here.
PDF is available here.
Abstract
Atherosclerotic plaque disruption accounts for the major part of cardiovascular mortality and the risk of disruption appears to depend on plaque composition. Carotid plaques in patients, scheduled for endarterectomy, have been successfully characterised with MRI. MRI has the advantage of combining i...
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PMID: 20058083
PDF is available here.
PDF is available here.
Investigative Radiology (45)2 2010
Abstract
VEGFR2-targeted ultrasound contrast agents such as BR55 will likely prove useful in human for the early detection of tumors as well as for the assessment of response to specific treatments....
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PMID: 20027118
PDF is available here.
PDF is available here.
Abstract
We developed a common oligonucleotide reference standard (CORS) method to address this problem. A unique 50-mer reference oligonucleotide probe was selected to co-spot with gene probes for each array feature. The complementary sequence was synthesized and labeled for use as the reference target, whi...
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PMID: 20038701
PDF is available here.
PDF is available here.
Abstract
We review these chemical tools and their application to unraveling the molecular basis of synaptic plasticity....
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PMID: 20081822
PDF is available here.
PDF is available here.
Abstract
We developed multiplexed assays for rapid, simultaneous pathogen detection and characterization of ciprofloxacin and doxycycline resistance in Bacillus anthracis, Yersinia pestis, and Francisella tularensis. These assays are SNP-based and use Multiplexed Oligonucleotide Ligation-PCR (MOL-PCR). The M...
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PMID: 20865530
PDF is available here.
PDF is available here.
Abstract
Our results suggest that the common submicroscopic "genomic disorders" (microdeletion and microduplication syndromes) would not be frequently detected in the first trimester anomalies screening.
Copyright 2009 Elsevier Masson SAS. All rights reserved....
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PMID: 20004752
PDF is available here.
PDF is available here.
Abstract
We have performed array-CGH analysis of 36 Swedish bladder exstrophy patients. The analysis revealed a similar and approximately 3 Mb duplication, consistent with the recently described 22q11.2 microduplication syndrome, in two unrelated cases with bladder exstrophy and hearing impairment. This find...
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PMID: 20045748
PDF is available here.
PDF is available here.
Abstract
We identified one CBE patient with an overlapping 22q11.21 duplication in whom the duplication had been transmitted from the unaffected mother. Chromosomal region 22q11 is well known for its susceptibility to genomic rearrangements, and these are associated with various syndromes including the velo-...
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PMID: 20060941
PDF is available here.
PDF is available here.
Abstract
Radiation therapy is one of the effective curative treatments for uterine cervical cancer. However poor clinical results for the advanced stages require further improvement of the treatment. Intensive studies on basic and clinical research have been made to improve local control, primarily important...
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PMID: 20173313
PDF is available here.
PDF is available here.
Abstract
Atomic force microscope (AFM) has been used incrementally over the last decade in cell biology. Beyond its usefulness in high resolution imaging, AFM also has unique capabilities for probing the viscoelastic properties of living cells in culture and, even more, mapping the spatial distribution of ce...
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PMID: 20463929
PDF is available here.
PDF is available here.
Abstract
Abstract
We present a review of optical manipulation, emphasizing its impact on the areas of single-molecule studies and single-cell biology, and indicating some of the key experiments in the fields....
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PMID: 20799781
PDF is available here.
PDF is available here.
Abstract
We exploit the sensitivity offered by surface-enhanced Raman scattering (SERS) for the direct detection of thrombin using the thrombin-binding aptamer (TBA) as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding...
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PMID: 20799837
PDF is available here.
PDF is available here.
Bioconjugate Chemistry (21)1 2010
Abstract
We use surface plasmon resonance (SPR) to directly and quantitatively study the affinity and binding kinetics of nanoparticles that display small molecules conjugated to their surface. We studied the interaction between a single protein target and a structurally related series of targeting ligands w...
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PMID: 20028085
PDF is available here.
PDF is available here.
Abstract
Molecular imaging is a new and evolving field that employs a targeted approach to noninvasively assess biologic processes in vivo. By assessing key elements in specific cellular processes prior to irreversible end-organ damage, molecular tools will allow for earlier detection and intervention, impro...
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PMID: 20012514
PDF is available here.
PDF is available here.
Optics Express (17)26 2009
Abstract
We examined. However, in the absence of modeling inaccuracies and low noise levels, the maximum likelihood estimator is more accurate than the nonlinear least squares estimator, as measured by the standard deviations of its estimates, and attains the best possible accuracy achievable for the sets of...
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PMID: 20052043
PDF is available here.
PDF is available here.
Abstract
Abstract
An aptamer-participated haprin structure was designed by employing cellular prion protein (PrP(C)) as a model protein, and thus an aptamer-mediated turn-on fluorescence assay for proteins was developed in this contribution. The designed aptamer-participated haprin structure consists of three segment...
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PMID: 19635360
PDF is available here.
PDF is available here.
Abstract
The present study suggests that apoptosis and MMP are interrelated in atherosclerotic lesions and the targeting of more than one molecular candidate is feasible by molecular imaging....
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PMID: 19662466
PDF is available here.
PDF is available here.
Abstract
We will discuss the optical properties of pyrene excimer and summarize recent progresses on pyrene excimer signaling nucleic acid probes for the signaling of biomolecules and their interactions in complex biological samples....
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PMID: 20201423
PDF is available here.
PDF is available here.
Journal of Biophotonics (2)10 2009
Abstract
We investigated the potential of green fluorescent nanodiamonds as biomarkers in living cells. Nanodiamonds were functionalized by attaching antibodies that target intracellular structures such as actin filaments and mitochondria. Then, the nanodiamond conjugates were transfected into HeLa cells. Tr...
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PMID: 19504515
PDF is available here.
PDF is available here.
Optics Express (17)20 2009
Abstract
We present experimental and theoretical results of label-free molecular sensing using the transverse magnetic mode of a 0.22 mum thick silicon slab waveguide with a surface grating implemented in a guided mode resonance configuration. Due to the strong overlap of the evanescent field of the waveguid...
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PMID: 19907628
PDF is available here.
PDF is available here.
Euro surveillance : bulletin europeen sur les maladies transmissibles = European communicable disease bulletin (14)38 2009
Abstract
In 2007 in The Netherlands, 30% of all human isolates of methicillin-resistant Staphylococcus aureus (MRSA) sent to the National Institute for Public Health and the Environment could not be typed by pulsed-field gel electrophoresis (non-typable (NT)-MRSA). Molecular characterisation of the NT-MRSA i...
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PMID: 19814956
PDF is available here.
PDF is available here.
Abstract
We labeled two populations of beta-cells within the developing zebrafish pancreas. These populations originate in distinct pancreatic buds and exhibit gene expression profiles suggesting distinct functions during development. We find that the dorsal bud derived beta-cells are quiescent and exhibit a...
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PMID: 19706417
PDF is available here.
PDF is available here.
Molecular Biotechnology (43)1 2009
Abstract
Macromolecular drugs hold great promise as novel therapeutics of several major disorders, such as cancer and cardiovascular disease. However, their use is limited by lack of efficient, safe, and specific delivery strategies. Successful development of such strategies requires interdisciplinary collab...
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PMID: 19475521
PDF is available here.
PDF is available here.
Nature Methods (6)10 2009
Abstract
We developed genetically encoded fluorescence resonance energy transfer (FRET)-based sensors that display a large ratiometric change upon Zn(2+) binding, have affinities that span the pico- to nanomolar range and can readily be targeted to subcellular organelles. Using this sensor toolbox we found t...
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PMID: 19718032
PDF is available here.
PDF is available here.
Molecular and Cellular Biochemistry (328)1-2 2009
Abstract
We investigated the applicability of fluorescent probes for their detection (i) in rabbit lens epithelial cells during ageing in culture, and (ii) in thin sections of rat heart. We used dihydroethidium (DHE), dichlorofluorescin (DCFH), and dihydrorhodamine 123 (DHR) together with detection of autofl...
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PMID: 19301099
PDF is available here.
PDF is available here.
Abstract
A solid-state electrochemiluminescence (ECL) biosensing switch system based on special ferrocene-labeled molecular beacon aptamer (Fc-MBA) has been developed successfully for thrombin detections. Such special switch system includes two main parts, an ECL substrate and an ECL intensit...
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PMID: 19442509
PDF is available here.
PDF is available here.
Abstract
In this paper, a microarray-based surface-enhanced Raman spectroscopic (SERS) assay for detection of kinase functionality and inhibition has been reported. Biotinylated anti-phosphoserinen antibodies mark the phosphorylation and inhibition events and gold nanoparticles are attached t...
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PMID: 19464160
PDF is available here.
PDF is available here.
Abstract
At this time, the pathophysiology of macrophage involvement and their role in stroke progression are poorly understood. Recently, T2- and T2*-weighted magnetic resonance imaging (MRI), after intravenous administration of iron-oxide particles, have been used to understand the inflammatory cascade. Ea...
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PMID: 19417758
PDF is available here.
PDF is available here.
Abstract
We explored whether MMP activity can be visualized by noninvasive near-infrared fluorescence (NIRF) imaging using an MMP-activatable probe in a mouse model of stroke. C57Bl6 mice were subjected to transient middle cerebral artery occlusion (MCAO) or sham operation. Noninvasive NIRF imaging was perfo...
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PMID: 19417756
PDF is available here.
PDF is available here.
Abstract
We report the results of SPECT/CT of CXCR4 expression levels in experimental brain tumors using (125)I-labeled anti-CXCR4 monoclonal antibodies (mAbs)....
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PMID: 19525448
PDF is available here.
PDF is available here.
Abstract
The tracer was eliminated quickly from the blood and normal tissues, providing high tumor-to-blood and tumor-to-muscle ratios as early as 20 min after injection. The high-contrast images between normal and tumor tissue were recorded for BT474 and MCF7/clone18 tumors. Low but still detectable uptake...
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PMID: 19525458
PDF is available here.
PDF is available here.
Oncology Reports (22)1 2009
Abstract
Our findings demonstrate the effectiveness and feasibility of preparing an effective mAb-based dual functional imaging agent for PET and NIRF targeting the CD20 expression in lymphoma....
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PMID: 19513512
PDF is available here.
PDF is available here.
Analytical Biochemistry (393)2 2009
Abstract
We have developed an ultrasensitive analytical method based on surface-enhanced Raman spectroscopy (SERS) exploiting a Raman probe covalently bound to gold nanoparticles. The biological marker to be detected was adsorbed on functionalized gold nanoparticles. The capture of these nanoparticles via a...
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PMID: 19563767
PDF is available here.
PDF is available here.
Der Orthopäde (38)6 2009
Abstract
The diagnosis of infections in patients with arthritis and/or joint prostheses requires interdisciplinary cooperation and the use of up-to-date methods. Massive bacterial infection can be identified by bacterial culture, and minimal infection can be detected by molecular pathological methods. These...
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PMID: 19455307
PDF is available here.
PDF is available here.
Pediatric Radiology (39 Suppl 3)s3 2009
Abstract