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All › Diagnosis › Diagnostic Techniques and Procedures › Diagnostic Imaging › Microscopy › Microscopy, Fluorescence

Microscopy, Fluorescence (1)

    Articles on Microscopy, Fluorescence

    1. Quantitative single-cell characterization of bacterial interactions reveals type VI secretion is a double-edged sword.
      Michele M Leroux, Justin A JA De Leon, ... Joseph D JD Mougous

      Proc Natl Acad Sci U S A 109(48):19804-9 (2012) PMID 23150540

      We developed time-lapse fluorescence microscopy methods for quantitation of interbacterial interactions and applied these to the characterization of type VI secretion (T6S) in Pseudomonas aeruginosa. Our analyses allowed a direct determination of the efficiency of recipient cell lysis catalyzed by t...
    2. Control of solid tumor growth in mice using EGF receptor-targeted RNA replicase-based plasmid DNA.
      B Leticia BL Rodriguez, Xinran X Li, ... Zhengrong Z Cui

      Nanomedicine (Lond) 7(4):475-91 (2012) PMID 22296186

      Delivery of pSIN-β using the EGF receptor-targeted liposome more effectively controlled the growth of MDA-MB-468 tumors (and human epidermoid carcinoma A431 tumors) in mice than using untargeted liposome. The pSIN-β carried by the EGF receptor-targeted liposome caused the complete regression of MD...
    3. 3D coupling of fibronectin fibril arrangement with topology of ventral plasma membrane.
      Hiroaki H Hirata, Chwee Teck CT Lim, and Hidetake H Miyata

      Cell Commun Adhes 19(2):17-23 (2012) PMID 22409236

      We report a novel and simple method based on total internal reflection fluorescence microscopy to quantify the distance between the ventral plasma membrane and the glass substratum. We observe that the distance varies from  80 nm in other regions. Furthermore, by applying this novel method, we sho...
    4. An ICT-based strategy to a colorimetric and ratiometric fluorescence probe for hydrogen sulfide in living cells.
      Fabiao F Yu, Peng P Li, ... Keli K Han

      Chem Commun (London) 48(23):2852-4 (2012) PMID 22293939

      We present a colorimetric and ratiometric fluorescent probe Cy-N(3) that exhibits a selective response to H(2)S. The probe employs a near-infrared cyanine as a fluorophore, and is equipped with an operating azide unit. It is readily employed for assessing intracellular H(2)S levels, and confocal rat...
    5. Modulation of noncanonical TGF-β signaling prevents cleft palate in Tgfbr2 mutant mice.
      Jun-ichi Iwata, Joseph G Hacia, ... Yang Chai

      J Clin Invest 122(3):873-85 (2012) PMID 22326956

      We show that loss of Tgfbr2 in mouse cranial neural crest cells results in elevated expression of TGF-β2 and TGF-β receptor type III (TβRIII); activation of a TβRI/TβRIII-mediated, SMAD-independent, TRAF6/TAK1/p38 signaling pathway; and defective cell proliferation in the palatal mesenchyme. St...
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    6. A caged, localizable rhodamine derivative for superresolution microscopy.
      Sambashiva S Banala, Damien D Maurel, ... Kai K Johnsson

      ACS Chem Biol 7(2):289-93 (2012) PMID 22026407

      We demonstrate here....
    7. Origin of the brush cell lineage in the mouse intestinal epithelium.
      Matthew M Bjerknes, Cyrus C Khandanpour, ... Hazel H Cheng

      Dev Biol 362(2):194-218 (2012) PMID 22185794

      We report that brush cell numbers increase dramatically following conditional Atoh1-deletion, demonstrating that brush cell production, determination, differentiation and survival are Atoh1-independent. We also report that brush cells are derived from Gfi1b-expressing progenitors. These and related...
    8. A chromenoquinoline-based fluorescent off-on thiol probe for bioimaging.
      Dnyaneshwar D Kand, Arunasree Marasanapalli AM Kalle, ... Pinaki P Talukdar

      Chem Commun (London) 48(21):2722-4 (2012) PMID 22301487

      A new chromenoquinoline-based fluorescent off-on thiol probe 2 is reported. In aqueous buffer solutions at physiological pH, the probe exhibited 223-fold enhancement in fluorescence intensity by a Michael addition of cysteine to the maleimide appended to a chromenoquinoline. Cell per...
    9. Role of urokinase plasminogen activator receptor-associated protein in mouse lung.
      Michael M MM Bundesmann, Teresa E TE Wagner, ... Lynn M LM Schnapp

      Am J Respir Cell Mol Biol 46(2):233-9 (2012) PMID 21940816

      We analyzed the pulmonary mechanics of uPARAP(-/-) and wild-type mice at baseline and examined their response after bleomycin instillation. We compared collagen internalization in primary mouse lung fibroblasts (MLFs) from wild-type and uPARAP(-/-) mice using flow cytometry and fluorescent microscop...
    10. Avian SERPINB11 gene: a marker for ovarian endometrioid cancer in chickens.
      Whasun Lim, Ji-Hye Kim, ... Gwonhwa Song

      Exp Biol Med (Maywood) 237(2):150-9 (2012) PMID 22289513

      We identified SERPINB11 as a novel estrogen-induced gene in chickens during oviduct development. The chicken is a unique animal model for research on human ovarian cancer, because it spontaneously develops epithelial cell-derived ovarian cancer as in women. Therefore, this study investigated the exp...