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Chloramphenicol Resistance (0):
Recent article
Chloramphenicol Resistance
Abstract
We have investigated the properties of a suspected hotspot for the integration of the mariner Mos1 element, namely the Tn9 cat gene that encodes a chloramphenicol acetyl transferase. Using in vitro and bacterial transposition assays, we confirmed that the cat gene is a preferential target for MOS1 i...
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PMID: 19112581
PDF is available here.
PDF is available here.
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BMC Microbiology (9)1 2009
Abstract
The SMc02161 locus in S. meliloti shows similarities with members of the Major Facilitator Superfamily (MFS) of transporters. A S. meliloti null-mutant shows increased sensitivity to chloramphenicol. This indication led us to rename the locus tep1 for transmembrane efflux protein. The lack of tep1 d...
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PMID: 19173735
PDF is available here.
PDF is available here.
Abstract
Chloramphenicol-resistant gene was cloned and analyzed by constructing genomic DNA library of Serratia marcescens KMR-3. It showed that cloned chloramphenicol-resistant gene encoded a protein product of 397 amino acids. The protein belonged to PRK10473 protein, and it showed 92% similarity to drug r...
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PMID: 18807985
PDF is available here.
PDF is available here.
Extremophiles (12)3 2008
Abstract
We developed an electroporation protocol and transformed seven isolates related to members of the Arthrobacter, Microbacterium, Curtobacterium, and Rhodoglobus genera with pSVJ21. Plasmid stability was demonstrated by successive transformation into Escherichia coli and four Gram-positive isolates, g...
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PMID: 18335166
PDF is available here.
PDF is available here.
Biomedical Research (29)1 2008
Abstract
We constructed a disrupted mutant of the put A gene, which encodes the proline utilization A (Put A) flavin-linked enzyme, in order to examine the role of put A in the gastric colonization of H. pylori. The put A disrupted mutant, DeltaputA, was constructed by inserting a chloramphenicol resistant g...
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PMID: 18344593
PDF is available here.
PDF is available here.
Abstract
Vaccination has not been used widely because of the interference in the discrimination between infected and vaccinated animals in immune-screening procedures. In the present study, chloramphenicol resistance gene (Cm(r)) was cloned into the genomic DNA of brucella suis S2 strain by homologous recomb...
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PMID: 18271249
PDF is available here.
PDF is available here.
Abstract
This method can be used for rapid construction of Camp. jejuni deletion mutants. SIGNIFICANCE AND IMPACT OF THE STUDY: The use of this method should facilitate functional characterization of various Camp. jejuni genes....
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PMID: 17908230
PDF is available here.
PDF is available here.
Abstract
In recent years mef genes, encoding efflux pumps responsible for M-type macrolide resistance, have been investigated extensively for streptococci. mef(I) is a recently described mef variant detected in particular isolates of Streptococcus pneumoniae instead of the more common mef(E) and mef(A). This...
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PMID: 17709462
PDF is available here.
PDF is available here.
Abstract
Our goal was to assess whether UPEC isolates resistant to chloramphenicol, tetracycline and streptomycin also exhibit reduced virulence potential. Among 110 human UPEC isolates, the prevalences of the virulence factors fimH, papC, papGII, papGIII, sfa/focDE, afa, hlyA, cnf1, usp, ibeA, fyuA, iroN, i...
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PMID: 17719751
PDF is available here.
PDF is available here.
Abstract
This is to the best of our knowledge the first report of a floR gene in B. bronchiseptica isolates. The identification of the first member of a new subclass of cml genes, cmlB1 from B. bronchiseptica, extends our knowledge on specific chloramphenicol exporters....
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PMID: 17224413
PDF is available here.
PDF is available here.
Molecular Microbiology (63)2 2007
Abstract
Enterococcus faecalis, a leading cause of nosocomial antibiotic resistant infections, frequently possesses a 150 kb pathogenicity island (PAI) that carries virulence determinants. The presence of excisionase and integrase genes, conjugative functions and multiple insertion sequence elements suggests...
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PMID: 17163979
PDF is available here.
PDF is available here.
Journal of Medical Microbiology (55)Pt 12 2006
Abstract
Salmonella enterica subspecies enterica serovar Paratyphi B [O1,4,(5),12 : Hb : 1,2] can cause either an enteric fever (paratyphoid fever) or self-limiting gastroenteritis in humans. The d-tartrate non-fermenting variant S. enterica subsp. enterica serovar Paratyphi B dT- (S. Paratyphi B) is the cau...
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PMID: 17108269
PDF is available here.
PDF is available here.
Abstract
We have modified this method by developing a set of 3 plasmid derivatives from the previously described suicide vector for allelic exchange, pKAS32, to make antibiotic marker exchange easier and thus accelerate the entire process. Briefly, the construction of each single gene knockout mutant is carr...
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PMID: 16750581
PDF is available here.
PDF is available here.
Abstract
These observations suggest that eefABC activation can occur in vitro independently of the H-NS, MarA or RamA global regulators....
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PMID: 16606635
PDF is available here.
PDF is available here.
Abstract
Molecular Microbiology (60)5 2006
Abstract
Four mutations resulting in opal stop codons were individually engineered into a plasmid-borne chloramphenicol-resistance (cat) gene driven by the lac promoter. These four mutations were located at different sites in secondary structures. The mutations were analysed with the computer program mfg, wh...
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PMID: 16689800
PDF is available here.
PDF is available here.
Revista Medica de Chile (134)4 2006
Abstract
As CM and CAF are active "in vitro", high rates of clinical response should be expected. In contrast, PNC is less effective, especially against pigmented Prevotella....
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PMID: 16758082
PDF is available here.
PDF is available here.
Abstract
We have constructed a live vaccine candidate, IEM108, which carries an El Tor-derived rstR gene. This gene encodes a repressor and can render bacterial resistance to CTXPhi infection. In this study, we evaluated the resistance of IEM108 against CTXPhi infection by using a CTXPhi marked for chloramph...
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PMID: 16343705
PDF is available here.
PDF is available here.
Abstract
The CP acetyltransferase gene (ie, cat1) was identified as the predominant CP-resistance gene in strains isolated from cattle, and the cat1and nonenzymatic CP-resistance gene (ie, cmlA) were the predominant CP-resistance genes in strains isolated from pigs. Additionally, strains with cat1 isolated f...
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PMID: 16454626
PDF is available here.
PDF is available here.
Abstract
We previously reported construction of a shuttle plasmid, pHS17, using the native fusobacterial plasmid pFN1 and an erythromycin resistance cassette. However, the host range of pHS17 was restricted to F. nucleatum, ATCC 10953, and the transformation efficiency was limited. This study was undertaken...
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PMID: 16115683
PDF is available here.
PDF is available here.
Abstract
Influence of chloramphenicol on electrophysiologic charateristics of Escherichia coli strains susceptible (K-12 strain) and resistant (pBR-325 strain) to it has been studied. It has been shown that incubation of susceptible bacteria with chloramphenicol leads to significant change of magnitute of el...
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PMID: 17297873
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (175)1 2006
Abstract
The open reading frame (ORF) in the Pseudomonas aeruginosa chromosome, whose product resembles the chloramphenicol acetyltransferases (CAT) belonging to the CATB family, was cloned and shown to confer resistance to chloramphenicol (Cm) in Escherichia coli. The determinant was therefore named catB7 an...
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PMID: 10361706
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (79)2-3 2006
Abstract
We successfully transformed Enterococcusfaecium, E. hirae, E. malodoratus and E. mundtii....
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PMID: 1905659
PDF is available here.
PDF is available here.
Abstract
A 5.1-kb plasmid, designated pSCS12, isolated from a naturally occurring Staphylococcus sciuri conferred resistance to chloramphenicol (CmR) and streptomycin (SmR). Restriction endonuclease analyses of pSCS12 revealed partial structural homologies to the CmR-plasmids pC221 from S. aureus and pSCS1 fr...
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (170)1 2006
Abstract
Epidemic strain Salmonella typhimurium DT104 is characterized by various multiresistance patterns. At least some of the resistance genes are organized as integrons. Resistance genes of DT104 isolates can be efficiently transduced by P22-like phage ES18 and by phage PDT17 which is released by all DT10...
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PMID: 9919675
PDF is available here.
PDF is available here.
Abstract
Transformation studies were carried out on 16 clinically isolated Cm-resistant (250 microg/mL of Cm) E coli strains by using pEGFP-C1-EGScat1cat2 recombinant plasmid. Transformants were screened on LB-agar plates containing Km after the transformation using EGS. Of the 16 tested strains, 4 strains w...
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PMID: 16437645
PDF is available here.
PDF is available here.
Abstract
A strain of Aeromonas hydrophila producing copolyesters of 3-hydroxybutyrate and 3-hydroxyhexanoate, abbreviated as PHBHHx, was successfully transformed by electroporation. The plasmid used was a broad host range plasmid pBBR1MCS. Electroporation conditions were varied systemically to develop an ele...
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PMID: 15967502
PDF is available here.
PDF is available here.
Abstract
This is the first report of trimethoprim, chloramphenicol and sulphonamide resistance genes and class 1 integrons in B. bronchiseptica isolates....
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PMID: 16046466
PDF is available here.
PDF is available here.
Abstract
Lactobacillus johnsonii FI9785, a strain originally isolated from poultry gastrointestinal tract for its probiotic function as a competitive excluder of pathogens, was found to contain two cryptic plasmids of 3.5 and 25.6 kb. Nucleotide sequence analysis of the entire small plasmid, designated p9785...
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PMID: 16122563
PDF is available here.
PDF is available here.
Abstract
Antimicrobial resistance patterns of Salmonella enterica serovar Typhimurium isolates obtained during the study period were examined. The molecular epidemiology and the mechanisms of resistance to ampicillin, chloramphenicol and tetracycline were investigated. Resistance to ampicillin increased from...
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PMID: 16122913
PDF is available here.
PDF is available here.
Veterinary Microbiology (109)1-2 2005
Abstract
An active chloramphenicol efflux system was demonstrated in a multiresistant E. coli isolated from poultry carcass. The effect of different concentrations of chloramphenicol on the original strain and on the plasmid-cured strain was determined in the presence and in the absence of CCCP, an uncoupler...
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PMID: 15963664
PDF is available here.
PDF is available here.
Abstract
We confirmed a computer-predicted transmembrane topological model of FloR, using the phoA gene fusion method, and classified this protein in the DHA12 family (containing 12 transmembrane domains) of MF efflux transporters. We also showed that FloR is a transporter specific for structurally associate...
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PMID: 15980376
PDF is available here.
PDF is available here.
Abstract
The florfenicol/chloramphenicol resistance gene floR was found to be part of the novel 4,284-bp transposon TnfloR from Escherichia coli. TnfloR consists of the gene floR, a putative regulatory gene, and the transposase gene tnpA. A circular form of TnfloR was detected and suggested the potential mob...
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PMID: 15855539
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (245)2 2005
Abstract
Escherichia coli isolates from calf diarrhea cases (n=22) in the Beijing surrounding region in China were characterized for disease serotype, virulence factors, antimicrobial susceptibility pattern and class 1 integrons. 59% (n=13) of the isolates were positive for the int I1 gene. The presence and...
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PMID: 15837385
PDF is available here.
PDF is available here.
Abstract
Abstract
The MICs for 162 diarrheagenic Escherichia coli strains and 28 Shigella strains were determined on the basis of NCCLS guidelines. More than 75% of the strains were resistant to ampicillin, chloramphenicol (53.6% of Shigella strains), and trimethoprim-sulfamethoxazole. Multiresistance was detected in...
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PMID: 15673777
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (243)1 2005
Abstract
These results suggest that in the absence of specific chloramphenicol selection pressure, the cmlA gene is maintained by virtue of gene linkage to genes encoding resistance to antimicrobials that are currently approved for use in food animals....
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PMID: 15668031
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (240)2 2004
Abstract
Mycobacterium tuberculosis and Mycobacterium bovis are inhibited by chloramphenicol. Chloramphenicol acetyltransferase (CAT) converts chloramphenicol to inactive diacetyl chloramphenicol, but a mycobacterial carboxylesterase hydrolyzes the diacetyl product to active chloramphenicol. The esterase act...
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PMID: 15522506
PDF is available here.
PDF is available here.
Microbiology (150)Pt 11 2004
Abstract
The nucleotide sequences of the broad-host-range antibiotic resistance plasmids pB2 (61 kb) and pB3 (56 kb), which were isolated from a wastewater treatment plant, were determined and analysed. Both have a nearly identical IncP-1beta backbone, which diverged early from the sequenced IncP-1beta plasm...
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PMID: 15528648
PDF is available here.
PDF is available here.
Journal of Medical Microbiology (53)Pt 11 2004
Abstract
Recent studies have shown that Clostridium difficile strains with variant toxins and those with resistance to macrolide-lincosamide-streptogramin B (MLSB) are increasingly causing severe disease and outbreaks in hospital settings. Here, the pathogenicity locus (PaLoc), the acquisition of binary toxi...
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PMID: 15496392
PDF is available here.
PDF is available here.
Abstract
We present an overview of the different mechanisms responsible for resistance to Cm and Ff with particular focus on the two different types of chloramphenicol acetyltransferases (CATs), specific exporters and multidrug transporters. Phylogenetic trees of the different CAT proteins and exporter prote...
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PMID: 15539072
PDF is available here.
PDF is available here.
Abstract
This study evaluated the relationship between florfenicol resistance and flo genotypes in 1,987 Escherichia coli isolates from cattle. The flo gene was detected in 164 isolates, all of which expressed resistance to florfenicol at MICs of >/=256 microg/ml. The florfenicol MICs for all isolates that l...
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PMID: 15388477
PDF is available here.
PDF is available here.
Abstract
We assessed the mechanisms of resistance to macrolide-lincosamide-streptogramin B (MLS(B)) antibiotics and related antibiotics in erythromycin-resistant viridans group streptococci (n = 164) and Gemella spp. (n = 28). The macrolide resistance phenotype was predominant (59.38%); all isolates with thi...
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PMID: 15328112
PDF is available here.
PDF is available here.
Pediatrics in Review (25)8 2004
Abstract
Molecular Microbiology (53)4 2004
Abstract
Site-specific recombination is an important mechanism for genetic exchange. Insertional recombination mediated by the recently delineated large resolvase or serine recombinase proteins is unique within the resolvase family as integration was thought to be a reaction catalysed only by members of the...
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PMID: 15306021
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (236)2 2004
Abstract
A chloramphenicol acetyltransferase (catB7) gene containing two point mutations, 181A/G and 314A/G, has been recently reported to be a determinant for high-level chloramphenicol resistance phenotype in a Pseudomonas aeruginosa strain PAhcr1. The mutant CATB7 was further characterized in vitro and in...
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PMID: 15251197
PDF is available here.
PDF is available here.
Molecular Microbiology (51)6 2004
Abstract
Chloramphenicol resistance in Clostridium perfringens and Clostridium difficile is often encoded by catP genes located within the 6.3 kb integrative mobilizable elements Tn4451 and Tn4453 respectively. This family of transposons is capable of being mobilized into a recipient cell in the presence of...
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PMID: 15009902
PDF is available here.
PDF is available here.
Abstract
Development of resistance of viridans streptococci in the normal flora to vancomycin and chloramphenicol during prophylactic use with uneventful cataract surgery is unlikely; the effect on resistance patterns of other antimicrobials is minor. Routine use of prophylactic vancomycin is discouraged, ho...
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PMID: 15030817
PDF is available here.
PDF is available here.
Abstract
We show that SetR, an SXT encoded repressor, represses the expression of activators of SXT transfer. The 'SOS response' to DNA damage alleviates this repression, increasing the expression of genes necessary for SXT transfer and hence the frequency of transfer. SOS is induced by a variety of environm...
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PMID: 14688795
PDF is available here.
PDF is available here.
Abstract
This cloth-based hybridization array system (CHAS) provides a simple, cost-effective tool for monitoring S. Typhimurium DT104 in foods and their production environment. SIGNIFICANCE AND IMPACT OF THE STUDY: The CHAS is a simple and cost-effective tool for the simultaneous detection of amplicons gene...
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PMID: 15214723
PDF is available here.
PDF is available here.
Abstract
We describe two distinct serogroup B strains isolated in Australia in 1994 and 1997, and found among approximately 1400 invasive meningococcal isolates examined in Australia over a 9 year period. Both were phenotypically chloramphenicol resistant on disc, Etest and agar inclusion MIC and acetylated...
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PMID: 14563894
PDF is available here.
PDF is available here.
Abstract
We focus on the molecular biology of antimicrobial resistance mediated by specific efflux systems and highlight the association of the respective resistance genes with mobile genetic elements and their distribution across species and genus borders....
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PMID: 13678822
PDF is available here.
PDF is available here.
The Lancet (361)9368 2003
Abstract
Abstract
pMC524/MBM, which codes for C resistance could be transferred into some C sensitive clinical strains of S. aureus. The size and the RE digestion patterns of the plasmids isolated from the S. aureus transformants were identical to those of pMC524/MBM. INTERPRETATION & CONCLUSION: These results sugges...
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PMID: 14604302
PDF is available here.
PDF is available here.
Abstract
A new transposon, designated TnSs1, which contains a chloramphenicol acetyltransferase gene flanked by direct repeats of an IS6-family element was found in a field isolate of Streptococcus suis. Polymerase chain reaction and hybridization analyses indicated that another field isolate carried the sam...
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PMID: 12726767
PDF is available here.
PDF is available here.
Abstract
Transferable resistance to various drugs was investigated in Photobacterium damselae subsp. piscicida from Japan. Drug resistances were transferred via plasmids of 100, 50, and 40 kb. Resistance to chloramphenicol (Cmr) was transferred on plasmids of all 3 sizes. The Cmr gene (cat) was cloned from t...
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PMID: 12650243
PDF is available here.
PDF is available here.
La Tunisie medicale (81)2 2003
Abstract
676 E. faecalis strains were listed over a period of 2 years from the Charles Nicolle hospital of Tunis (167 strains), the Habib Bourguiba hospital of Sfax (350 strains) and the National Centre of Bone marrow Transplantation of Tunis (159 strains). Antibiotic sensibility study was realized by the me...
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PMID: 12708176
PDF is available here.
PDF is available here.
Abstract
A high rate of antibiotic resistance was associated with the Salmonella isolates and the genes responsible for the resistance are located on conjugative plasmids. Also, there appears to be minimal diversity associated with the isolates. SIGNIFICANCE AND IMPACT OF THE STUDY: As a result of the increa...
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PMID: 12534821
PDF is available here.
PDF is available here.
Abstract
In a search for non-Shine-Dalgarno (non-SD) translational initiators, two combinatorial expression libraries (denoted R(1) and R(2)) were constructed containing randomized decanucleotide regions placed at either 6 (R(1)) or 11 (R(2)) nucleotides upstream of a modified chloramphenicol acetyltransfera...
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PMID: 12766344
PDF is available here.
PDF is available here.
Abstract
The plasmid-encoded R/M system LlaAI significantly improves the bacteriophage resistance of L. lactis during milk fermentations. SIGNIFICANCE AND IMPACT OF THE STUDY: It is essential to determine the potential of a phage defence mechanism in L. lactis starter culture strains during growth in milk be...
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PMID: 12680948
PDF is available here.
PDF is available here.