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Kanamycin Resistance (0):
Recent article
Kanamycin Resistance
Journal of wound care (19)7 2010
Abstract
Approximately 51% of total bacteria in the wound exudate with identical colony morphotype were resistant to 100 microg/ml of ampicillin. A single colony from this population also demonstrated resistance to 50 microg/ml of kanamycin on kanamycin-supplemented agar. Further antimicrobial sensitivity te...
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PMID: 20616773
PDF is available here.
PDF is available here.
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Abstract
We report on the cloning and characterization of a novel B. oleracea PMEI gene, BoPMEI1. Heterologously expressed BoPMEI1 showed PMEI activity. RT-PCR studies of different tissues and promoter-GUS fusions confirmed that BoPMEI1 was specifically expressed in mature pollen grains and pollen tubes. Bas...
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PMID: 20229192
PDF is available here.
PDF is available here.
Abstract
Many microorganisms produce extracellular polymers referred to collectively as "slime" or glycocalyx, and form biofilms on solid surfaces in natural ecosystems. Campylobacter jejuni, one of the most important foodborne pathogens, also has the ability to form biofilm on stainless steel, glass, or pol...
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PMID: 20009353
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)1 2010
Abstract
We screened 125 DNA bar-coded mutants to identify novel cell envelope stress and acute acid shock phenotypes associated with deletions of genes coding for sRNAs and small proteins. Nine deletion mutants (ssrA, micA, ybaM, ryeF, yqcG, sroH, ybhT, yobF, and glmY) were sensitive to cell envelope stress...
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PMID: 19734312
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)1 2010
Abstract
These results show that gonococcal MMC repairs mismatches and small insertion/deletions in DNA and also affects the recombination events underlying pilin antigenic variation. The differential effects of MMC and uvrD in gonococci unexpectedly reveal that MMC can function independently of uvrD in this...
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PMID: 19854909
PDF is available here.
PDF is available here.
FASEB Journal (23)1 2009
Abstract
We have used in vitro transcription of the kan gene by the T7 RNA polymerase (RNAP) in the presence of AID and a genetic reversion assay for kanamycin-resistance to investigate the causes of multiple clustered mutations (MCMs) during somatic hypermutations. We find that, depending on transcription c...
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PMID: 18772346
PDF is available here.
PDF is available here.
Abstract
Attenuated B. pertussis strains 5 and 35 containing mutant operon ptx and gene of resistance to canamycin were engineered. Recombinant bacteria retained marker of resistance to canamycin as well as structure of mutant operon during cultivation on growth media and long-term survival in lung of labora...
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PMID: 20095432
PDF is available here.
PDF is available here.
Abstract
We developed a Himar1-based random mutagenesis system for B. pseudomallei (HimarBP). The transposons contain a Flp recombinase-excisable, approved kanamycin resistance selection marker and an R6K origin of replication for transposon rescue. In vivo mutagenesis of virulent B. pseudomallei strain 1026...
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PMID: 18952878
PDF is available here.
PDF is available here.
Mutation Research (643)1-2 2008
Abstract
These results support a two-tiered model where RecQ facilitates fork progression through triplex-forming tracts and, failing processivity, RecG is critical for replication fork restart....
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PMID: 18582477
PDF is available here.
PDF is available here.
Journal of Bacteriology (190)14 2008
Abstract
We have compared the transcriptome and proteome profiles of the wild-type (WT) and mutant PrrA2 cells grown anaerobically in the dark with dimethyl sulfoxide as an electron acceptor. Approximately 25% of the genes present in the PrrA2 genome are regulated by PrrA at the transcriptional level, either...
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PMID: 18487335
PDF is available here.
PDF is available here.
Abstract
A locus (kmr) responsible for aminoglycosides-resistance of Sorangium cellulosum was cloned and characterized in Myxococcus xanthus. The gene kmr encodes a putative rRNA methyltransferase. Expression of the complete ORF endowed the Myxococcus transformants with the resistance to aminoglycosidic anti...
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PMID: 18355448
PDF is available here.
PDF is available here.
Tuberculosis (88)3 2008
Abstract
In sequel to previous report [Srivastava V, Rouanet C, Srivastava R, Ramalingam B, Locht C, Srivastava BS. Macrophage-specific Mycobacterium tuberculosis genes: identification by green fluorescent protein and kanamycin resistance selection. Microbiology 2007;153:659-66], the genes of Mycobacterium t...
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PMID: 18054522
PDF is available here.
PDF is available here.
Abstract
We have constructed a temperature-sensitive Tn5-based transposon delivery system that is capable of generating chromosomal reporter fusions with lacZ or luxCDABE, enabling us to monitor gene expression. Transposition is catalyzed by the hyperactive Tn5 transposase, whose expression is driven by the...
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PMID: 18344342
PDF is available here.
PDF is available here.
Molecular Microbiology (67)5 2008
Abstract
We show that, by two mechanisms, during natural transformation of Acinetobacter baylyi two IR events can integrate DNA segments. One mechanism is double illegitimate recombination (DIR) acting in the absence of any homology (frequency: 7 x 10(-13) per cell). It occurs about 10(10)-fold less frequent...
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PMID: 18194157
PDF is available here.
PDF is available here.
Abstract
Peanut allergy is one of the most life-threatening food allergies and one of the serious challenges facing the peanut and food industries. Current proposed solutions focus primarily on ways to alter the immune system of patients allergic to peanut. However, with the advent of genetic engineering nov...
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PMID: 17784907
PDF is available here.
PDF is available here.
Abstract
Conformational analysis predicts that 18 triplets cannot form the correct structure to function as the -1 triplet of tmRNA. We tested the tmRNA activity of all possible -1 triplet mutants using a genetic assay in Escherichia coli. While many mutants displayed reduced activity, our findings do not ma...
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PMID: 18590561
PDF is available here.
PDF is available here.
Abstract
We have devised a simple and rapid subcloning strategy to transfer any DNA fragment of interest from an entry clone into an expression vector, without this shortcoming. The strategy is based on the use of type IIs restriction enzymes, which cut outside of their recognition sequence. With proper desi...
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PMID: 18985154
PDF is available here.
PDF is available here.
Mikrobiologiia (77)3 2008
Abstract
The effect of kanamycin on the electrophysical parameters of cell suspensions of Escherichia coli K-12 and pMMB33 was investigated. Incubation of the sensitive K-12 strain with kanamycin resulted in significant changes in the orientation spectra (OS) of the cell suspensions; these changes were not r...
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PMID: 18683656
PDF is available here.
PDF is available here.
Mutation Research (624)1-2 2007
Abstract
We show that the hot gene is not essential. Based on its promoter structure, hot has been previously classified as a "late" phage gene, a property that is not easily reconciled with a presumed replication function. Here, we clarify this issue by demonstrating that P1 hot is actively expressed both d...
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PMID: 17482649
PDF is available here.
PDF is available here.
Plant Cell Reports (26)9 2007
Abstract
The monocot family Lemnaceae (duckweed) is composed of small, edible, aquatic plants. Spirodela oligorrhiza SP is a duckweed with a biomass doubling time of about 2 days under controlled, axenic conditions. Stably transformed Spirodela plants were obtained following co-cultivation of regenerative ca...
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PMID: 17492286
PDF is available here.
PDF is available here.
Abstract
A spontaneous rpsL mutant of Thermus thermophilus was isolated in a search for new selection markers for this organism. This new allele, named rpsL1, encodes a K47R/K57E double mutant S12 ribosomal protein that confers a streptomycin-dependent (SD) phenotype to T. thermophilus. Models built on the a...
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PMID: 17601820
PDF is available here.
PDF is available here.
Abstract
We focused on the identification of the CMN biosynthetic gene cluster in this bacterium. Here, we describe the cloning and sequence analysis of the CMN biosynthetic gene cluster from S. mutabilis subsp. capreolus ATCC 23892. We provide evidence for the heterologous production of CMN in the genetical...
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PMID: 17496129
PDF is available here.
PDF is available here.
Abstract
We previously identified a 477 base pair (bp) region of DNA 147 bp upstream of lipF that is transcriptionally upregulated by exposure to growth media at pH 4.5 [Saviola, B., Woolwine, S., Bishai, W. R., 2003. Isolation of acid-inducible genes of Mycobacterium tuberculosis with the use of recombinase...
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PMID: 17434691
PDF is available here.
PDF is available here.
Journal of Molecular Biology (369)1 2007
Abstract
We use site-specific mutagenesis, coupled with calorimetric, NMR, and enzymological techniques, to define the key interactions that govern the binding of the aminoglycoside antibiotics neomycin and kanamycin B to APH(3')-IIIa (an antibiotic phosphorylating enzyme that confers resistance). Our mutati...
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PMID: 17418235
PDF is available here.
PDF is available here.
Abstract
A pair of bifunctional expression vectors, pBL-WZX and pHY-WZX, for Escherichia coli and Bacillus licheniformis was constructed to express interesting genes in a secretory manner. The vectors contain an expression cassette consisted of the promoter and signal peptide region of B. licheniformis amyL...
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PMID: 17256153
PDF is available here.
PDF is available here.
Microbiology (153)Pt 3 2007
Abstract
Mycobacterium tuberculosis survives and multiplies inside macrophages of its host by modulating the expression of several genes essential for in vivo survival. An in vivo expression system has been developed, based on green fluorescent protein and kanamycin resistance, to identify M. tuberculosis ge...
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PMID: 17322185
PDF is available here.
PDF is available here.
Molecular Immunology (44)5 2007
Abstract
Activation-induced cytidine deaminase (AID) is likely responsible for DNA cytidine deamination, although it may also act as an RNA deaminase. It functions on single-stranded DNA, the non-template strand in double-stranded DNA during transcription, or both strands in supercoiled DNA. To ask whether A...
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PMID: 16697045
PDF is available here.
PDF is available here.
Abstract
Our observations suggest the contents of the GIT shield or adsorb DNA, preventing detectable exposure of feed-derived DNA fragments to competent bacteria....
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PMID: 17961488
PDF is available here.
PDF is available here.
Abstract
We tested four newly prepared constructs based on the pGreen vector system containing the selection gene nptII or bar under the 35S or nos promoter. All these vectors carried gus under 35S. We also tested the pDM805 vector, with the bar and gus genes respectively under the Ubi1 and Act1 promoters, w...
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PMID: 17160584
PDF is available here.
PDF is available here.
Abstract
We were able to demonstrate before that the repair of a single double-strand break (DSB) in a plant genome can be mutagenic due to insertions and deletions. However, during replication or due to irradiation several breaks might be induced simultaneously. To analyze the mutagenic potential of such a...
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PMID: 17057227
PDF is available here.
PDF is available here.
Abstract
We have modified this method by developing a set of 3 plasmid derivatives from the previously described suicide vector for allelic exchange, pKAS32, to make antibiotic marker exchange easier and thus accelerate the entire process. Briefly, the construction of each single gene knockout mutant is carr...
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PMID: 16750581
PDF is available here.
PDF is available here.
FEBS Letters (580)21 2006
Abstract
Perturbation of the aerobic steady-state in a chemostat culture of the ethanol-producing bacterium Zymomonas mobilis with a small pulse of ethanol causes a burst of ethanol oxidation, although the reactant ratio of the alcohol dehydrogenase (ADH) reaction ([NADH][acetaldehyde][H(+)])/([ethanol][NAD(...
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PMID: 16950260
PDF is available here.
PDF is available here.
Plant Cell Reports (25)8 2006
Abstract
A protocol for Agrobacterium-mediated transformation with either kanamycin or mannose selection was developed for leaf explants of the cultivar Prunus dulcis cv. Ne Plus Ultra. Regenerating shoots were selected on medium containing 15 muM kanamycin (negative selection), while in the positive selecti...
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PMID: 16534597
PDF is available here.
PDF is available here.
Abstract
Acinetobacter sp. BD413 was transformed with DNA derived from GM potato and GM papaya, in the presence of an inactivated nptII gene on a plasmid. However, the frequency of such events in the natural environment on wild-type strains, while evidently low, remains unknown. SIGNIFICANCE AND IMPACT OF TH...
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PMID: 16869908
PDF is available here.
PDF is available here.
Abstract
A sarA disrupted strain named S. epidermidis RP62Adelta sarA was constructed, which was completely defective in biofilm formation, while the sarA complement strain RP62Adelta sarA (pHPS9sarA) restored the biofilm formation phenotype. Additionally, the knockout of sarA resulted in decreased erythromy...
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PMID: 16810749
PDF is available here.
PDF is available here.
Abstract
To investigate the putative five-gene operon at 24.9 min on the Escherichia coli genome, which comprises the genes pabC, yceG, tmk, holB and ycfH, a method for the construction of an in frame deletion strain of the essential E. coli holB gene was developed. HolB, also referred to as delta prime or d...
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PMID: 16083981
PDF is available here.
PDF is available here.
Abstract
This research reports the sensitivity of a clinical isolate of Enterococcus faecalis to sodium N-lauroylsarcosinate (sarkosyl) and sodium dodecyl sulfate (SDS), as well as the efficiency of these detergents in curing the strain. Compared to Escherichia coli, Enterococcus faecalis was very sensitive...
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PMID: 15988583
PDF is available here.
PDF is available here.
Abstract
We report a 32-year-old woman who presented with infectious keratitis in the right eye 3 weeks after laser in situ keratomileusis (LASIK). On microbiological investigations, the microorganism isolated was Pseudomonas aeruginosa that was sensitive to ciprofloxacin. To our knowledge, this is the only...
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PMID: 16631068
PDF is available here.
PDF is available here.
Abstract
Nocardia keratitis may not respond to 2% topical amikacin and fortified topical 5% amikacin may cause a strong toxic-allergic reaction. A commercially available combination of chloramphenicol, tetracycline, and colistin, with ofloxacin, may be effective but the treatment must be continued for severa...
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PMID: 16496263
PDF is available here.
PDF is available here.
Nucleic Acids Research (34)13 2006
Abstract
We have suggested previously that a possible way in which cells may prevent or reduce this chemical reaction is through the binding of proteins to DNA. We use a genetic reversion assay to show that a restriction enzyme, PspGI, protects cytosines within its cognate site, 5'-CCWGG (W is A or T), again...
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PMID: 16893959
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (72)1-2 2006
Abstract
The restriction deficient mutant 8195 of Acholeplasma laidlawii strain JA1 was transformed by the promiscuous streptococcal plasmid vector pNZ18 at a frequency of 4 × 1022124/cfu. The plasmid was maintained without structural rearrangements but was lost in the absence of a selection pressure, i.e. k...
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PMID: 2283030
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (69)1-2 2006
Abstract
The chimeric plasmid pMY10 containing the origin of replication of pAL5000, the origin of replication of pBR322, the origin of transfer of pRK2 and a kanamycin resistance gene was constructed and succesfully transferred by conjugation from Escherichia coli harbouring the helper plasmid pRK4.24 into M...
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PMID: 2199300
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (182)1 2006
Abstract
An aerotaxis gene, aer, was cloned from Pseudomonas putida PRS2000. A P. putida aer mutant displayed an altered aerotactic response in a capillary assay. Wild-type P. putida clustered at the air/liquid interface. In contrast, the aer mutant did not cluster at the interface, but instead formed a diffu...
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PMID: 10612751
PDF is available here.
PDF is available here.
Biochemistry (Washington) (44)50 2005
Abstract
We investigate a possible heme-propionate involvement in redox-coupled proton transfer by a combination of specific (13)C-heme propionate labeling and Fourier transform infrared (FTIR) difference spectroscopy. The labeling was achieved by creating a W. succinogenes mutant that was auxotrophic for th...
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PMID: 16342962
PDF is available here.
PDF is available here.
Abstract
We investigate the roles of transcription and DNA sequence in cytosine deamination. Strikingly different results are found with different substrates. Depending on the target sequence, the transcribed DNA strand is targeted as well as or better than the nontranscribed strand. The preferential targeti...
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PMID: 16314506
PDF is available here.
PDF is available here.
Abstract
We have developed a gene disruption cassette, which incorporates the Kanr marker, and direct repeat sequences designed from the target gene to enable the deletion of the gene without the introduction of added DNA sequences. We report on the disruption of the HO gene as a test case, using the hodr-Ka...
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PMID: 15949856
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (251)1 2005
Abstract
We report the identification and nucleotide sequence analysis of a cryptic plasmid pMIDG2830 from the Gram-negative bacterium Neisseria flavescens. The largest open reading frame encodes a protein similar to the replication protein, RepA, found in pAB49 from Acinetobacter baumannii and pNI10 from Ps...
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PMID: 16112819
PDF is available here.
PDF is available here.
Abstract
We isolated a small multicopy cryptic plasmid, pNHK101, from Thermus sp. TK10 for use as a replicon of a Thermus expression vector. The nucleotide sequence of pNHK101 revealed that this plasmid was 1564bp long, with a total G+C content of 66.8%, which was in agreement with that of Thermus genomic DN...
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PMID: 15907540
PDF is available here.
PDF is available here.
Chromosoma (114)1 2005
Abstract
We report the successful induction of chromosome translocations in unmodified yeast cells via targeted DNA integration of the KAN(R) selectable marker flanked by sequences homologous to two chromosomal loci randomly chosen on the genome. Using this bridge-induced translocation system, 2% of the inte...
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PMID: 15843952
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (245)2 2005
Abstract
A procedure for high-efficiency gene inactivation in Bacillus anthracis has been developed. It is based on a highly temperature-sensitive plasmid vector carrying kanamycin resistance cassette surrounded by DNA fragments flanking the desired insertion site. The approach was tested by constructing glu...
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PMID: 15837388
PDF is available here.
PDF is available here.
Abstract
Nucleic Acids Research (33)5 2005
Abstract
We developed a set of cassettes and plasmids useful for creating targeting vectors to modify the mammalian genome. A positive selection marker cassette (PGK/EM7p-npt), which included dual prokaryotic and eukaryotic promoters to permit consecutive selection for recombination in Escherichia coli and t...
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PMID: 15784610
PDF is available here.
PDF is available here.
Analytical Biochemistry (335)2 2004
Abstract
New Tn10 minitransposons were constructed to functionally map long-range transcription regulatory sequences in bacterial artificial chromosomes (BACs) and P1-derived artificial chromosomes (PACs). Each contained a wild-type loxP site but, significantly, contained no mammalian or bacterial genes and/...
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PMID: 15556570
PDF is available here.
PDF is available here.
Archives of Microbiology (182)6 2004
Abstract
The KgmB methylase (the kanamycin-gentamicin resistance methylase from Streptomyces tenebrarius) acts at G-1405 of 16S rRNA within the sequence CGUCA that is also found 6 bp in front of ribosomal binding site of the kgmB gene. The kgmBColon, two colonslacZ gene and operon fusions were used in order...
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PMID: 15578257
PDF is available here.
PDF is available here.
Veterinary Microbiology (104)1-2 2004
Abstract
We have constructed attenuated, capsule-deficient mutants of A. pleuropneumoniae that contain a kanamycin resistance (Kn(R)) gene within the capsule locus of the genome. Following intranasal or intratracheal challenge of pigs the encapsulated parent strains colonized the challenge pigs, and were tra...
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PMID: 15530740
PDF is available here.
PDF is available here.
Journal of Biotechnology (114)3 2004
Abstract
Several non-Watson Crick DNA structures have been discovered to date, which may be incorporated into future plasmid constructs for gene therapy and DNA vaccine products. In this study, intrinsic DNA structures were included at a defined point in a 2.9 kb plasmid, and their effects on cell growth rat...
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PMID: 15522434
PDF is available here.
PDF is available here.
Journal of Bacteriology (186)22 2004
Abstract
The expression of an antisense RNA revealed that an Mn-catalase was required in Thermus thermophilus for aerobic but not for anaerobic growth. The antisense system is based on the constitutive expression of a "bicistronic" transcript consisting of the kanamycin resistance gene mRNA followed by the a...
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PMID: 15516595
PDF is available here.
PDF is available here.
Plant Physiology (135)2 2004
Abstract
We describe a reliable method for the screening and selection of Arabidopsis transgenic seeds within minutes without germination. Expression of the Aspergillus niger beta-glucosidase gene BGL1 in the plant's endoplasmic reticulum was used as a visual marker, together with 4-methylumbelliferyl-beta-D...
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PMID: 15208418
PDF is available here.
PDF is available here.
Abstract
We have generated non-polar mutants in each of the genes by inserting an aphA3 cassette encoding kanamycin resistance. All three mutants show similar phenotypes: the mutants are unable to grow on minimal medium under iron deficiency conditions. Furthermore, regardless of iron conditions, all mutants...
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PMID: 15142249
PDF is available here.
PDF is available here.
Abstract
Colonization with Helicobacter pylori always results in chronic gastritis, which is controlled by infiltration of mononuclear cells and the subsequent release of cytokines like interleukin (IL)-12. To identify H. pylori factors involved in inducing cytokine production in mononuclear cells, a random...
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PMID: 15145461
PDF is available here.
PDF is available here.