Abstract
We show that the combined action of Isw1 and Chd1 nucleosome-spacing enzymes is required to maintain this organization. In the absence of these enzymes, regular positioning of the majority of nucleosomes is lost. Exceptions include the region upstream of the promoter, the +1 nucleosome, and a subset...
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PMID: 21940898
PDF is available here.
Abstract
We used mathematical modeling and quantitative in vivo experiments to define the contributions of individual components of the G₁-S network downstream of Hog1 to this stress-induced delay in the cell cycle. The length of the arrest depended on the degree of stress and the temporal proximity of the...
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PMID: 21954289
PDF is available here.
Abstract
We describe 31 dominant-negative, secreted variant isoforms of receptor tyrosine kinases (RTKs) that are produced by activation of intronic poly(A) sites. We show that blocking U1-snRNP can activate IPA, indicating a larger role for U1-snRNP in RNA surveillance. Moreover, we report the development o...
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PMID: 21925381
PDF is available here.
Abstract
RNA molecules play diverse functional roles in natural biological systems. There has been growing interest in designing synthetic RNA counterparts for programming biological function. The design of synthetic RNA molecules that exhibit diverse activities, including sensing, regulatory...
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PMID: 21925380
PDF is available here.
Victor T Tchemtchoua,
Ganka Atanasova,
Abdel Aqil,
Patrice Filée,
Nancy Garbacki,
Olivier Vanhooteghem,
Christophe Deroanne,
Agnès Noël,
Christine Jérome,
Betty Nusgens,
Yves Poumay and
Alain Colige
Abstract
The final goal of the present study was the development of a 3-D chitosan dressing that would shorten the healing time of skin wounds by stimulating migration, invasion, and proliferation of the relevant cutaneous resident cells. Three-dimensional chitosan nanofibrillar scaffolds pro...
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PMID: 21761871
PDF is available here.
Abstract
I and elastin was significantly up-regulated when compared with TCPS control, whereas collagen III was down-regulated on PHBV fibers and 2-D film after 14 days in culture. The less ductile PHBV 2-D films showed higher levels of elastin expression. Furthermore, the PHBV fibers in the presence and abs...
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PMID: 21800891
PDF is available here.
Abstract
We show that protein arginine methylation limits the ERK1/2 signal elicited by particular growth factors in different cell types from various species. We found that this restriction in ERK1/2 phosphorylation depended on methylation of RAF proteins by protein arginine methyltransferase 5 (PRMT5). PRM...
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PMID: 21917714
PDF is available here.
Abstract
We describe a protocol to differentiate murine Tol-DCs from bone marrow precursors in vitro. Importantly, Tol-DCs actively suppress T cells stimulated with immunogenic allogeneic DCs. Indeed, Tol-DCs generated using this approach can be useful in studying and characterising the immunoregulatory path...
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PMID: 20941609
PDF is available here.
Abstract
We have shown, both in vitro and in vivo, that NF-κB blockade on DCs by andrographolide or rosiglitazone can significantly enhance the tolerogenic capacity of DCs. Furthermore, we have observed that expression ratio of the activating FcγRIII or the inhibitory FcγRIIb is determinant for the tolero...
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PMID: 20941620
PDF is available here.
Circulation (124)11 Suppl 2011
Abstract
We previously showed that adenoviral-delivered overexpression of an endogenous inhibitor, the tissue inhibitor of metalloproteinases-3 (TIMP-3), significantly retarded intimal thickening in short-term autologous porcine arteriovenous interposition grafts (28 days). However, it is essential to determ...
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PMID: 21911803
PDF is available here.
Circulation (124)11 Suppl 2011
Mei Huang,
Patricia Nguyen,
Fangjun Jia,
Shijun Hu,
Yongquan Gong,
Patricia E de Almeida,
Li Wang,
Divya Nag,
Mark A Kay,
Amato J Giaccia,
Robert C Robbins and
Joseph C Wu
Abstract
We performed a cell trafficking experiment, which demonstrated significantly greater recruitment of bone marrow cells to the ischemic myocardium in the double-knockdown group. Fluorescence-activated cell sorting showed significantly higher activation of endogenous c-kit(+) cardiac progenitor cells....
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PMID: 21911818
PDF is available here.
Circulation (124)11 Suppl 2011
Abstract
We used bioinformatics analysis and experimental validation assays to show that Bim, a critical apoptotic activator, is an important target gene of the microRNA cocktail, which collectively can bind to the 3'UTR region of Bim and suppress its expression.
We have demonstrated that a m...
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PMID: 21911815
PDF is available here.
Circulation (124)11 Suppl 2011
Abstract
We investigated the effect of epidermal growth factor-like domain 7 (Egfl7) on nuclear factor-κB activation, intercellular adhesion molecule-1 expression, and neutrophil adhesion to human coronary artery endothelial cells after calcineurin-inhibition-induced injury.
Human coronary e...
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PMID: 21911813
PDF is available here.
Abstract
We recently characterized the interaction between the intraviral domains of envelope glycoproteins (Gn and Gc) and ribonucleoprotein (RNP) of Puumala and Tula hantaviruses (genus Hantavirus, family Bunyaviridae). Herein we report a direct interaction between spike-forming glycoprotein and nucleic ac...
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PMID: 21807393
PDF is available here.
Abstract
We used functional multineuron calcium imaging to monitor the spike outputs from thousands of CA1 neurons in response to the stimulation of two independent sites of the dentate gyrus in rat hippocampal networks ex vivo. Only pyramidal cells were analyzed based on post hoc immunostaining. Some CA1 py...
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PMID: 21917800
PDF is available here.
Abstract
We used two-photon RuBi-Glutamate uncaging to optically map how the largest population of cortical interneurons, the parvalbumin-positive cells (PV+), are connected to pyramidal cells (PCs) in mouse neocortex. We found locally dense connectivity from PV+ interneurons onto PCs across cortical areas a...
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PMID: 21917809
PDF is available here.
Abstract
We contrasted the connection patterns of parvalbumin-immunoreactive cells in two functionally distinct cortical regions: the tonotopic, narrowly frequency-tuned module [central narrow band (cNB)] of cat central primary auditory cortex (AI) and the nontonotopic, broadly tuned second auditory field (A...
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PMID: 21917816
PDF is available here.