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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Biochemistry (7):
Recent article
Biochemistry
Biophysics (40)1 2011
Abstract
I was able to participate in the great adventure of the last half of the twentieth century: the revolution in biology that advanced the field of protein chemistry to so great an extent. The future is bright and limitless, with greater understanding of biology yet to come....
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PMID: 21545283
PDF is available here.
PDF is available here.
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Abstract
Analytical Biochemistry (411)1 2011
Abstract
Guanidinium thiocyanate-phenol-chloroform extraction (GTPC extraction) is widely used in molecular biology for isolating DNA, RNA, and proteins. Protein isolation by commercially available GTPC solutions is time consuming and the resulting pellets are only incompletely soluble. In th...
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PMID: 21094121
PDF is available here.
PDF is available here.
Abstract
Nature Methods (8)2 2011
Abstract
We introduce the Network-Free Stochastic Simulator (NFsim), a general-purpose modeling platform that overcomes the combinatorial nature of molecular interactions. Unlike standard simulators that represent molecular species as variables in equations, NFsim uses a biologically intuitive representation...
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PMID: 21186362
PDF is available here.
PDF is available here.
Current protocols in protein science / editorial board, John E. Coligan ... [et al.] (Chapter 11) 2011
Abstract
Two enzymatic methods commonly used in N-terminal sequence analysis of blocked proteins are presented: one uses pyroglutamate aminopeptidase for N(α)-pyrrolidone carboxyl-proteins in solution or blotted onto a membrane, and the other uses acylaminoacyl-peptide hydrolase for N(α)-ac...
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PMID: 21400688
PDF is available here.
PDF is available here.
Abstract
Abstract
Biochimica et Biophysica Acta (1808)3 2011
Abstract
These results show the new detergents as promising tools to expand the arsenal for membrane protein studies....
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PMID: 21130727
PDF is available here.
PDF is available here.
Abstract
Abstract
We performed purification before renaturation in the prokaryotic expression system instead of using the traditional renaturation-before-purification approach. rhBMP-2 was separated on a Sephacryl S-300 HR column and eluted from a DEAE-Sepharose Fast Flow column. The collected protein was refolded by...
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PMID: 19823948
PDF is available here.
PDF is available here.
Kidney International (78)10 2010
Abstract
Kidney International (76) 2010
Abstract
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Optics Express (18)18 2010
Abstract
We report a novel high-speed Fourier Fluorescence Excitation Emission spectrometer, which simultaneously measures three projections of EEM from a FRET sample, which are excitation, emission and excitation-emission cross-correlation spectra. We demonstrate that these three EEM projections can be meas...
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PMID: 20940777
PDF is available here.
PDF is available here.
Optics Express (18)18 2010
Abstract
We demonstrate an optical gain of ~200 cm(-1) for the mode under consideration, which corresponds to ~32% compensation of SPP loss....
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PMID: 20940755
PDF is available here.
PDF is available here.
Abstract
Chemical biology contributions will become increasingly important as we enter the second decade of the postgenomic era.
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PMID: 20720543
PDF is available here.
PDF is available here.
Abstract
Abstract
Excitatory synapses are located in confined chemical spaces called the dendritic spines. These are atypical femtoliter-order microdomains where the behavior of even single molecules may have important biological consequences. Powerful chemical biological techniques have now been deve...
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PMID: 20644538
PDF is available here.
PDF is available here.
Abstract
We report that PKSE chemistry does not direct 9- versus 10-membered enediyne core biosynthetic divergence as revealed by comparing the products from three 9-membered and two 10-membered PKSE-TE systems under identical conditions using robust in vivo assays. Three independent experiments support a co...
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PMID: 20534556
PDF is available here.
PDF is available here.
Abstract
We present a theory that generalizes the Arrhenius equation to include static disorder of conformational degrees of freedom as a function of an external perturbation to fully account for a diverse set of transition states. The effect of a perturbation on static disorder is best examined at the singl...
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PMID: 20534507
PDF is available here.
PDF is available here.
Abstract
We have compared the subunit composition of Mediator in three different structural states: bound to the activator SREBP-1a, VP16, or an activator-free state. As expected, consensus Mediator subunits were similarly represented in each sample. However, we identify a set of cofactors that interact spec...
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PMID: 20534441
PDF is available here.
PDF is available here.
Abstract
In this protocol, active anaphase promoting complex/cyclosome (APC/C) and its inhibitor, mitotic checkpoint complex (MCC), are purified from HeLa cell extracts. The two complexes are first separated by gel filtration and ion exchange chromatography. The MCC is further purified by an additional gel f...
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PMID: 20516187
PDF is available here.
PDF is available here.
Abstract
After any RNA purification by any method, it is necessary to ascertain both the amount (yield) and the integrity (quality) of the RNA obtained. This is essential for any subsequent analysis and critical for any comparative analyses. This article discusses several methods for RNA quantitation and pro...
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PMID: 20516190
PDF is available here.
PDF is available here.
Abstract
Biochimica et Biophysica Acta (1803)6 2010
Abstract
Ribosome biogenesis is a fundamental process that provides cells with the molecular factories for cellular protein production. Accordingly, its misregulation lies at the heart of several hereditary diseases (e.g., Diamond-Blackfan anemia). The process of ribosome assembly comprises the processing an...
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PMID: 19879902
PDF is available here.
PDF is available here.
Abstract
Slowing down of the relaxation of the fluctuations around equilibrium is investigated both by stochastic simulations and by analysis of master equation of reversible reaction networks consisting of reactions between a pair of resource and the corresponding high-energy product that works as a catalys...
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PMID: 20866274
PDF is available here.
PDF is available here.
Abstract
We discuss the biochemical three-dimensional reaction-diffusion model, which does not provide temporal self-sustained oscillations via reaction terms. However, the self-sustained oscillations and waves could be obtained using the proper boundary conditions for systems with a finite thickness. We hav...
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PMID: 20866284
PDF is available here.
PDF is available here.
Abstract
We report the purification of the TSC1 complex under physiological conditions using a proteomic strategy. We purified the TSC1 protein complex using a tandem affinity purification method and identified a protein complex containing 139 components. Two known binding proteins of TSC1 (TSC2 and DOCK7) w...
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PMID: 20383465
PDF is available here.
PDF is available here.
Journal of Biochemistry (147)4 2010
Abstract
Esmond E. Snell (1914-2003) was a giant of B-vitamin and enzyme research. His early research in bacterial nutrition had lead to the discovery of vitamins such as lipoic acid and folic acid, and an anti-vitamin avidin. He developed microbiological assay methods for riboflavin and other vitamins and a...
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PMID: 20353954
PDF is available here.
PDF is available here.
Chemical Communications (46)10 2010
Abstract
We summarize the recent progress in the development of bioorthogonal reactions and their applications in various biological systems. A major emphasis has been placed on the mechanistic and kinetic studies of these reactions with the hope that continuous improvements can be made with each reaction in...
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PMID: 20177591
PDF is available here.
PDF is available here.
Audio, Transactions of the IRE Professional Group on (Chapter 15) 2010
Quantitative analysis of endocytosis and turnover of epidermal growth factor (EGF) and EGF receptor.
Abstract
Binding of epidermal growth factor (EGF) to the EGF receptor (EGFR) initiates signal transduction, ultimately leading to altered gene expression. Ligand-activated EGFR is also rapidly internalized and then targeted to lysosomes for degradation or recycled back to the plasma membrane. Endocytosis is...
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PMID: 20235100
PDF is available here.
PDF is available here.
Abstract
Abstract
We introduce several methods to examine the interaction of transcriptional factors with and their sequestration by expanded polyglutamine proteins in vitro and in vivo....
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PMID: 20700715
PDF is available here.
PDF is available here.
Abstract
We describe new methods for the detection and analyses of N-linked, and the recently discovered free oligosaccharides (fOS), from whole cell lysates of Campylobacter jejuni using non-specific pronase E digestion and permethylation followed by mass spectrometry. We also describe the expression and im...
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PMID: 19882132
PDF is available here.
PDF is available here.
Abstract
We have developed a new glycomics strategy based on lectin-affinity capture of lipid-linked oligosaccharides (LLOs) coupled to capillary electrophoresis mass spectrometry. The LLO intermediates of the C. jejuni Pgl pathway were used to validate the methodology and to better characterize the bacteria...
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PMID: 19882129
PDF is available here.
PDF is available here.
Abstract
We describe a synthetic approach to polyacrylamide-based glycoconjugates with biotin tag, with special emphasis to development of carbohydrate biosensors and arrays....
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PMID: 19882121
PDF is available here.
PDF is available here.
Abstract
We sequenced and characterized the O-antigen biosynthetic gene cluster of Escherichia coli serotype O86. Four glycosyltransferases encoded by the genes within the cluster were cloned and overexpressed. In vitro reconstitution of the O-repeating unit of E. coli 086 was achieved via using these enzyme...
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PMID: 19882123
PDF is available here.
PDF is available here.
Abstract
Glycomics which is the study of saccharides and genes responsible for their formation requires the continuous development of rapid and sensitive methods for the identification of glycan structures. It involves glycoanalysis which relies upon the development of methods for determining the structure a...
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PMID: 19882127
PDF is available here.
PDF is available here.
Abstract
We have shown that respiratory defects cause oxidative damage in Afg3l2 mutants, indicating that oxidative stress is crucial in the pathogenesis of m-AAA deficiency....
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PMID: 20700718
PDF is available here.
PDF is available here.
Abstract
We detected significant levels of Bad in the mitochondria of monocytes/macrophages and found that these signaling pathways converge in Bad phosphorylation, and thus in its cytosolic accumulation. Phosphorylation inhibits binding of Bad to Bcl-2, or BclXL, and promotes its translocation to the cytoso...
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PMID: 20700721
PDF is available here.
PDF is available here.
Abstract
Genome Biology (11)1 2010
Abstract
We have developed NetPath as a resource of curated human signaling pathways. As an initial step, NetPath provides detailed maps of a number of immune signaling pathways, which include approximately 1,600 reactions annotated from the literature and more than 2,800 instances of transcriptionally regul...
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PMID: 20067622
PDF is available here.
PDF is available here.
Genome Biology (11)1 2010
Abstract
We could demonstrate that, depending on disease type, independent information from cell culture studies contrasts with conclusions drawn from patient studies....
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PMID: 20089154
PDF is available here.
PDF is available here.
Genome Biology (11)1 2010
Abstract
We analyzed four divergent Arabidopsis accessions and found that intersection of absent short read coverage with weak tiling array hybridization signal reliably flags deletions. Interestingly, individual deletions were frequently observed in two or more of the accessions examined, suggesting that va...
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PMID: 20067627
PDF is available here.
PDF is available here.
Abstract
We describe a case of a 69-year-old man with a pain at the base of right chest. On the basis of clinical evaluation, biochemical analysis, as well as imaging procedures, a diagnosis of right adrenocortical carcinoma was made. The patient underwent medical treatment....
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PMID: 20396632
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (82)1 2010
Abstract
Professor Olena Yakivna Rashba, doctor of science in biology, was born in Kyiv, to the physician's family; graduated from the Medical-Prophylactic Faculty of the First Medical Institute (1927-1931). Since 1933 she had been working at the Institute of Biochemistry of the Academy of Sciences of Ukr.SS...
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PMID: 20684238
PDF is available here.
PDF is available here.
Abstract
I had the time, I couldn't have done the experiments, because I don't know how. It's very much a team effort."...
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PMID: 20072108
PDF is available here.
PDF is available here.