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All › Investigative Techniques › Chemistry, Analytical › Chromatography › Chromatography, Liquid › Countercurrent Distribution

Countercurrent Distribution (0)

      Articles on Countercurrent Distribution

      1. Quality characteristics and fertilizing ability of ram sperm subpopulations separated by partition in an aqueous two-phase system.
        N N Mendoza, A A Casao, ... R R Pérez-Pé

        BIOMED APPL 880(1):74-81 (2012) PMID 22153331

        We have compared sperm quality parameters and the fertilizing ability of sperm subpopulations separated by the CCCD process from ram semen samples maintained at 20°C or cooled down to 5°C. Total and progressive sperm motility was evaluated by computer-assisted analysis using a CASA system and memb...
      2. Evaluation of the performance of protein separation in figure-8 centrifugal counter-current chromatography.
        Yi Y Yang, Dongyu D Gu, ... Yoichiro Y Ito

        BIOMED APPL 879(32):3802-5 (2011) PMID 22100551

        The performance of protein separation using the figure-8 column configuration in centrifugal counter-current chromatography was investigated under various flow rates and revolution speeds. The separation was performed with a two-phase solvent system composed of polyethylene glycol 10...
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      3. An efficient combination of supercritical fluid extraction and high-speed counter-current chromatography to extract and purify (E)- and (Z)-...
        Daijie D Wang, Yanling Y Geng, ... Luqi L Huang

        J Sep Sci 34(23):3339-43 (2011) PMID 22038778

        In this study, supercritical fluid extraction (SFE) was used to extract essential oil from Acorus tatarinowii Schott under the pressure of 25 MPa and temperature of 35°C. Two (E)- and (Z)-diastereomers of α-asarone and β-asarone were separated and purified by high-speed counter-cu...
      4. Preparative isolation and purification of antioxidative diarylheptanoid derivatives from Alnus japonica by high-speed counter-current chroma...
        Soon Sung SS Lim, Min Young MY Lee, ... Sang Hoon SH Jung

        J Sep Sci 34(23):3344-52 (2011) PMID 22083971

        This study employed the online HPLC-2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS)(+) bioassay to rapidly determine the antioxidant compounds occurring in the crude extract of Alnus japonica. The negative peaks of the ABTS(+) radical scavenging detection system, which...
      5. Preparative isolation and purification of senkyunolide-I, senkyunolide-H and ferulic acid from Rhizoma Chuanxiong using counter-current chro...
        Yun Y Wei, Jia J Hu, ... Jiangang J Liu

        J Sep Sci 34(23):3426-32 (2011) PMID 22052814

        Three active compounds, senkyunolide-I, senkyunolide-H and ferulic acid (FA), were successfully isolated and purified from the extracts of Rhizoma Chuanxiong by counter-current chromatography (CCC). Based on the principle of the partition coefficient values (k) for target compounds a...
      6. Extraction and preparative purification of tanshinones from Salvia miltiorrhiza Bunge by high-speed counter-current chromatography.
        Ailing Sun, Yongqing Zhang, ... Renmin Liu

        BIOMED APPL 879(21):1899-904 (2011) PMID 21621488

        A method for extraction and preparative separation of tanshinones from Salvia miltiorrhiza Bunge was successfully established in this paper. Tanshinones from Salvia miltiorrhiza Bunge were extracted using ethyl acetate as the extractant under reflux. The extracts were then purified b...
      7. [Simultaneous isolation and purification of gallic acid and brevifolincarboxylic acid from Polygonum capitatum by high-speed counter-current...
        Xinxia X Chen, Liyan L Zhang, ... Yu Y Xie

        Zhongguo Zhong Yao Za Zhi 35(15):1957-60 (2010) PMID 20931845

        To isolate and purify gallic acid and brevifolincarboxylic acid simultaneously by high-speed counter-current chromatography (HSCCC) from a crude extract of Polygonum capitatum. The biphasic solvent system composed of ethyl acetate-n-butanol-0.44% acetic acid (3:1:5) was used at a flow rate of 2.0 mL...
      8. Development of a fast and convenient method for the isolation of triterpene saponins from Actaea racemosa by high-speed countercurrent chrom...
        Serhat Sezai SS Cicek, Stefan S Schwaiger, ... Hermann H Stuppner

        Planta Med 76(5):467-73 (2010) PMID 19847744

        In the present work, a fast and simple method for the separation and purification of triterpene saponins from Actaea racemosa was successfully established. Accelerated solvent extraction was used for defatting and extracting of the subaerial parts, giving a triterpene enriched crude extract. Size ex...
      9. Isolation and purification of macrocyclic components from Penicillium fermentation broth by high-speed counter-current chromatography.
        Xiang X Gao, Rongqiang R Zhuang, ... Yufen Y Zhao

        J Sep Sci 33(2):277-84 (2010) PMID 20041450

        In this paper, high-speed counter-current chromatography (HSCCC), assisted with ESI-MS, was first successfully applied to the preparative separation of three macrolide antibiotics, brefeldin A (12.6 mg, 99.0%), 7'-O-formylbrefeldin A (6.5 mg, 95.0%) and 7'-O-acetylbrefeldin A (5.0 mg...
      10. [Research on technological process of two-pot countercurrent extraction of hydroxysafflor yellow A].
        Yerui Li, Yong Chen, ... Wenjie Yan

        Zhongguo Zhong Yao Za Zhi 34(21):2743-7 (2009) PMID 20209905

        OBJECTIVE: To study the optimum technical conditions of extracting Hydroxysafflor yellow A (HSYA) from Carthmus tinctorius by multi-stage countercurrent extraction technology. METHOD: The effects of extraction time of each stage, extraction temperature, ethanol concentration and solid-liquid ratio (...