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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Nucleic Acid Precursors (1):
Recent article
Nucleic Acid Precursors
Molecular Cell (38)4 2010
Abstract
We dissected this pathway in cells. We show that protein synthesis inhibition impairs the SMN complex, revealing discrete SMN and Gemin subunits and accumulating an snRNA precursor (pre-snRNA)-Gemin5 intermediate. By high-throughput sequencing of this transient intermediate's RNAs, we discovered the...
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PMID: 20513430
PDF is available here.
PDF is available here.
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Abstract
We succeeded in synthesizing DNA probes incorporating these thermo-removable protecting groups and developed a new system for molecular switching by changing the protection- and deprotection-modes using simple heating and re-carbamoylation with isocyanates. This reversible process enabled us to cont...
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PMID: 19194584
PDF is available here.
PDF is available here.
FEMS Yeast Research (8)8 2008
Abstract
We review eukaryotic pyrimidine degradation with emphasis on the contributions made with S. kluyveri and how this increases our understanding of human disease. Additionally, we discuss the possibilities and limitations of this nonconventional yeast as a laboratory organism....
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PMID: 18823354
PDF is available here.
PDF is available here.
Abstract
Mitochondrial DNA depletion syndrome (MDS), a reduction of mitochondrial DNA copy number, often affects muscle or liver. Mutations in enzymes of deoxyribonucleotide metabolism give MDS, for example, the mitochondrial thymidine kinase 2 (TK2) and deoxyguanosine kinase (dGK) genes. Sixteen TK2 and 22...
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PMID: 18600543
PDF is available here.
PDF is available here.
Abstract
FASEB Journal (21)10 2007
Abstract
Among the human diseases that result from abnormalities in mitochondrial genome stability or maintenance are several that result from mutations affecting enzymes of deoxyribonucleoside triphosphate (dNTP) metabolism. In addition, it is evident that the toxicity of antiviral nucleoside analogs is det...
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PMID: 17403938
PDF is available here.
PDF is available here.
Trends in Genetics (23)8 2007
Abstract
We understand which enzymes are involved in the primary metabolism of the cell? A recent study using comparative genomics and postgenomics approaches revealed a novel pathway in the most studied organism, Escherichia coli. The analysis of a new operon consisting of seven previously uncharacterized g...
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PMID: 17555842
PDF is available here.
PDF is available here.
BMC Evolutionary Biology (7 Suppl 2)Suppl 2 2007
Abstract
We review evidence showing that formamide in the presence of largely available catalysts and by moderate heating yields the complete set of nucleic bases necessary for the formation of nucleic acids. Formamide also favours the formation of acyclonucleosides and the phosphorylation and trans-phosphor...
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PMID: 17767725
PDF is available here.
PDF is available here.
Human Molecular Genetics (15)17 2006
Abstract
Our studies reveal a critical role of NR2E3 in establishing functional specificity of NRL-expressing photoreceptor precursors during retinal neurogenesis....
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PMID: 16868010
PDF is available here.
PDF is available here.
Abstract
Mammals have four deoxyribonucleoside kinases, the cytoplasmic (TK1) and mitochondrial (TK2) thymidine kinases, and the deoxycytidine (dCK) and deoxyguanosine (dGK) kinases, which salvage the precursors for nucleic acids synthesis. In addition to the native deoxyribonucleoside substrates, the kinase...
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PMID: 15896737
PDF is available here.
PDF is available here.
Abstract
We asked whether mitochondrial dNTP pools are asymmetric with respect to each other. We report here that the concentrations of the four dNTPs are not equal in mitochondria isolated from several tissues of both young and old rats. In particular, in most tissues examined, mitochondrial dGTP concentrat...
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PMID: 15784738
PDF is available here.
PDF is available here.
Chemical Society Reviews (33)4 2004
Abstract
Deoxyribonucleotides, monomers of macromolecular DNA and the chemical matter of genes, have received surprisingly little attention among chemists and molecular biologists alike, although their origin, properties, and mechanism of enzyme-catalyzed formation bear unique chemical traits which are the b...
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PMID: 15103404
PDF is available here.
PDF is available here.
FEBS Letters (560)1-3 2004
Abstract
Deoxyribonucleoside kinases, which catalyse the phosphorylation of deoxyribonucleosides, are present in several copies in most multicellular organisms and therefore represent an excellent model to study gene duplication and specialisation of the duplicated copies through partitioning of substrate sp...
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PMID: 14987989
PDF is available here.
PDF is available here.
Abstract
In addition to the primary and secondary structural elements in RNA, protein binding to substrate RNA can be a determinant of the RNase III cleavage site....
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PMID: 12814522
PDF is available here.
PDF is available here.
Gravitational and space biology bulletin : publication of the American Society for Gravitational and Space Biology (16)2 2003
Abstract
Exposure to the types of ionizing radiation encountered during space travel may cause a number of health-related problems, but the primary concern is related to the increased risk of cancer induction in astronauts. The major types of radiation considered to be of importance during space travel are p...
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PMID: 12959130
PDF is available here.
PDF is available here.
Trends in Genetics (19)6 2003
Abstract
We discuss the findings and what might be learned from this kind of approach....
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PMID: 12801718
PDF is available here.
PDF is available here.
Abstract
The new hypothesis of evolution establishes a contiguity of life sciences with cosmology, physics, and chemistry, and provides a basis for the search for life on other planets. Chemistry is the sole driving force of the assembly of life, under the subtle guidance exerted by bonding orbital geometry....
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PMID: 12382315
PDF is available here.
PDF is available here.
Abstract
Crosslinking of three different model nucleobases (9-ethyladenine, 9-EtA; 9-ethylguanine, 9-EtGH; 1-methyluracil, 1-MeU) by two linear trans-aPtII (a = NH3 or CH3NH2) entities leads to a flat metal-modified base triplet, trans,trans-[(NH3)2Pt(1-MeU-N3)(mu-9-EtA-N7,N1)Pt(CH3NH2)2(9-EtGH-N7)]3+ (4b)....
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PMID: 11405476
PDF is available here.
PDF is available here.
Molecular Microbiology (40)3 2001
Abstract
DNA uptake by naturally competent bacteria provides cells with both genetic information and nucleotides. In Haemophilus influenzae, competence development requires both cAMP and an unidentified signal arising under starvation conditions. To investigate this signal, competence induction was examined...
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PMID: 11359575
PDF is available here.
PDF is available here.
EMBO Reports (1)4 2000
Abstract
We constructed a network of nested introns. Introns were inserted in the 5' splice site and/or branchpoint of a synthetic yeast intron interrupting a reporter gene. The inserted introns mask the recipient intron from the cellular machinery until they are removed by splicing. Production of functional...
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PMID: 11269499
PDF is available here.
PDF is available here.
Journal of Molecular Biology (294)4 1999
Abstract
We show that P13 and P14 are the only native loop-loop interactions among six possible combinations. Other base-pairing interactions of the loop sequences stabilize misfolded and inactive pre-RNAs. Mismatches in P13 or P14 raised the midpoints and decreased the cooperativity of the Mg(2+)-dependent...
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PMID: 10588899
PDF is available here.
PDF is available here.
Biochemistry (Washington) (38)22 1999
Abstract
These results show that the diazoate is generated as a stable intermediate in the reactions of dCyd with HNO2 and NO and that the major product is the diazoate but not dUrd in the initial stage of the reactions. Thus, once formed in vivo, the diazoate persists for long time in DNA and may act as a m...
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PMID: 10353825
PDF is available here.
PDF is available here.
Journal of Molecular Biology (284)4 1998
Abstract
We have searched for yeast snoRNAs which are affected by the depletion of the yeast ortholog of bacterial RNase III, Rnt1. In a yeast strain inactivated for RNT1, almost half of the snoRNAs tested are depleted with significant accumulation of monocistronic or polycistronic precursors. snoRNAs from b...
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PMID: 9837720
PDF is available here.
PDF is available here.
Abstract
I polymorphism at intron 2 (the polymorphic site is identical for Hpa II restriction enzyme) or both Sma I and Sca I polymorphism at exon 3 of ANP precursor gene and essential hypertension. The aim of our study was to optimize the methods for Sma I and Sca I analysis in the ANP precursor gene (PCR f...
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PMID: 10085711
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (273)18 1998
Abstract
We have developed a new strategy for the evaluation of the mutagenicity of a damaged DNA precursor (deoxyribonucleoside 5'-triphosphate) in Escherichia coli. 8-Hydroxydeoxyguanosine triphosphate (8-OH-dGTP) and 2-hydroxydeoxyadenosine triphosphate (2-OH-dATP) were chosen for this study because they...
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PMID: 9556591
PDF is available here.
PDF is available here.
Abstract
Taq and Tth DNA polymerases catalyzed polymerization of dATP and dTTP into poly d(A-T) without requiring added primer/template (Hanaki et al., Biochem. Biophys. Res. Commun. 238, 113-118), while the Stoffel fragment of Taq DNA polymerase and delta Tth DNA polymerase with respective deletions of ca....
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PMID: 9514904
PDF is available here.
PDF is available here.
Abstract
A procedure was developed for mapping origins of DNA replication in mammalian cell chromosomes based on determining the relative abundance of nascent DNA strands throughout a specific genomic region. The method entails purification of short strands of nascent DNA derived from recently activated orig...
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PMID: 9441856
PDF is available here.
PDF is available here.
Abstract
We give a detailed description of the theory behind the method and a full recipe for its application. Advantages and limitations of the procedure are discussed....
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PMID: 9441855
PDF is available here.
PDF is available here.
Journal of Molecular Biology (270)2 1997
Abstract
The effects of deoxynucleoside triphosphate (dNTP) imbalances on the fidelity of human immunodeficiency virus type 1 (HIV-1) replication were investigated. Using detergent permeabilized virions and biased dNTP concentrations different types of hypermutants were readily produced. However, the mutant...
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PMID: 9236117
PDF is available here.
PDF is available here.
Abstract
We tested whether HRS expression correlates with the appearance of alternatively spliced fibronectin transcripts during liver growth. HRS was highly expressed during the proliferative phase of liver development, correlating with expression of the fibronectin EIIIB alternative exon. In regenerating l...
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PMID: 9199345
PDF is available here.
PDF is available here.
Abstract
These results suggest that Prp31p is involved in recruiting the U4/U6 x U5 tri-snRNP to prespliceosome complexes or in stabilizing these interactions....
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PMID: 9199293
PDF is available here.
PDF is available here.
Abstract
Ribosome biogenesis in eucaryotes involves many small nucleolar ribonucleoprotein particles (snoRNP), a few of which are essential for processing pre-rRNA. Previously, U8 snoRNA was shown to play a critical role in pre-rRNA processing, being essential for accumulation of mature 28S and 5.8S rRNAs. H...
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PMID: 9199304
PDF is available here.
PDF is available here.
Abstract
Regulation of both transcription and RNA splicing requires enhancer elements, that is, cis-acting DNA or RNA sequences that promote the activities of linked promoters or splice sites, respectively. Both types of enhancer associate with regulatory proteins to form multicomponent enhancer complexes th...
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PMID: 9159075
PDF is available here.
PDF is available here.
Molecular Microbiology (24)4 1997
Abstract
We show that DiSSU1 is mobile when haploid intron-containing and intron-less amoebae are mated. The mobility process is fast, being completed in 5-10 nuclear cycles after mating in the developing zygote and plasmodia. Analyses of progeny from genetic crosses confirm intron mobility. DiSSU1 is the fi...
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PMID: 9194701
PDF is available here.
PDF is available here.
Abstract
The activity of thymidine kinase, thymidine phosphorylase, adenosine deaminase and 5'-nucleotedase of AMP was studied in tissues, blood serum and lymphocytes of 60 healthy females and 50 females with fibrocavernous mastopathy aged 23-70. It was revealed that age-related changes in the activity of th...
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PMID: 9312943
PDF is available here.
PDF is available here.
Abstract
We report the purification of a 90-kDa double-stranded RNA-specific adenosine deaminase from HeLa cell nuclear extract that specifically edits the glutamine codon at position 586 in the pre-mRNA of the glutamate receptor B subunit. Site-specific deamination of an adenosine to an inosine converts the...
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PMID: 8995285
PDF is available here.
PDF is available here.
Abstract
We investigated ribonucleases from Saccharomyces cerevisiae which are active in pre-tRNA 3'-processing in vitro. Two pre-tRNA 3'-exonucleases with molecular masses of 33 and 60 kDa, two pre-tRNA 3'-endonucleases with molecular masses of 45 kDa/60 kDa and 55 kDa and 70-kDa 3'-pre-tRNase were purified...
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PMID: 9022706
PDF is available here.
PDF is available here.
Journal of Molecular Biology (264)5 1996
Abstract
The virulent Bacillus subtilis bacteriophage SPP1 packages its DNA from a precursor concatemer by a headful mechanism. Following disruption of mature virions with chelating agents the chromosome end produced by the headful cut remains stably bound to the phage tail. Cleavage of this tail-chromosome...
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PMID: 9000623
PDF is available here.
PDF is available here.
Abstract
It is very difficult to have a clear and homogeneous idea of the embryo metabolism. In fact it may vary from one species to another and also according to the embryonic stage: i.e. before and after genomic activation. Basic compounds such as glucose may be toxic, but obviously, it is more the problem...
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PMID: 8998511
PDF is available here.
PDF is available here.
Abstract
We have analyzed Chinese hamster ovary (CHO) cell mutants bearing nonsense codons in four of the five exons of the adenine phosphoribosyltransferase (aprt) gene and have found a pattern of mRNA reduction similar to that seen in systems studied previously: a decrease in steady-state mRNA levels of 5-...
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PMID: 8754843
PDF is available here.
PDF is available here.
EMBO Journal (15)11 1996
Abstract
tRNA-guanine transglycosylases (TGT) are enzymes involved in the modification of the anticodon of tRNAs specific for Asn, Asp, His and Tyr, leading to the replacement of guanine-34 at the wobble position by the hypermodified base queuine. In prokaryotes TGT catalyzes the exchange of guanine-34 with...
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PMID: 8654383
PDF is available here.
PDF is available here.
Abstract
To clarify the character of the anticoccidial activity of diclazuril a series of battery trials was conducted. Diclazuril showed excellent anticoccidial activity in the infection of chickens with Eimeria tenella, E. necatrix or E. acervulina at the feeding level of 0.1 ppm. When diclazuril was admin...
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PMID: 8672582
PDF is available here.
PDF is available here.
Abstract
The antagonistic phenomena to anticoccidial activities of 6-azauracil (AzU) induced by certain nucleic acid precursors were examined in battery experiments. Each nucleic acid precursor, orotate, orotidine, uracil, uridine, adenine and adenosine was mixed in feed in combination with effective levels...
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PMID: 8672581
PDF is available here.
PDF is available here.
Abstract
RNA editing corrects a 4C-A69 mismatch to a conventional 4T-A69 Watson-Crick base pair in the acceptor stem of the mitochondrially encoded tRNAPhe in plants. In vitro processing of edited and unedited Oenothera tRNA Phe precursor RNAs with pea mitochondrial protein extracts shows a significant effec...
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PMID: 8567636
PDF is available here.
PDF is available here.
Molecular Immunology (32)4 1995
Abstract
We show that RNA processing of chimeric mu alpha genes containing modifications in the C alpha 3 exon size and the C alpha 3-alpha m intron size respond to these modifications as predicted by previous mu gene studies. In addition, RNA expression ratios from the chimeric mu alpha genes are regulated...
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PMID: 7723773
PDF is available here.
PDF is available here.
Abstract
We report here that mutations within polyadenylation sequences that either decrease or increase the efficiency of RNA 3'-end formation have no effect on the efficiencies with which either the 3'-terminal or the penultimate intron is removed by splicing. Northern (RNA) blot hybridization, RNase mappi...
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PMID: 7799958
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (269)38 1994
Abstract
The involvement of exon sequences in splice site selection was studied in vivo in HeLa cells transfected with a series of model three exon-two intron pre-mRNAs which differed only in the sequence of their internal exons. When the majority of the human globin-derived 175-nucleotide internal exon (DUP...
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PMID: 8089127
PDF is available here.
PDF is available here.
Abstract
The translation of human triosephosphate isomerase (TPI) mRNA normally terminates at codon 249 within exon 7, the final exon. Frameshift and nonsense mutations of the type that cause translation to terminate prematurely at or upstream of codon 189 within exon 6 reduce the level of nuclear TPI mRNA t...
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PMID: 8065363
PDF is available here.
PDF is available here.
Abstract
In an effort to understand the mechanisms by which nonsense codons affect RNA metabolism in mammalian cells, nonsense mutations were generated within the gene for the secretory major urinary protein (MUP) of mice. The translation of MUP mRNA normally begins within exon 1 and terminates within exon 6...
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PMID: 8065364
PDF is available here.
PDF is available here.
Abstract
Ribozyme kinetics and binding studies of a two-piece group II intron were used to mechanistically characterize a reaction analogous to the first step of RNA splicing. Domain 5 RNA (D5) catalyzes specific hydrolysis of an RNA substrate (exD123) composed of sequences surrounding the 5' exon/intron bou...
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PMID: 8117737
PDF is available here.
PDF is available here.
Abstract
We describe apolipoprotein II (apoII) gene expression in cell lines derived by stable expression of the chicken estrogen receptor in LMH chicken hepatoma cells. In cell lines expressing high levels of receptor (LMH/2A), apoII gene expression is increased by estrogen 300-fold compared with levels in...
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PMID: 8114707
PDF is available here.
PDF is available here.
Abstract
We have investigated the function of different structural domains of the Drosophila splicing regulator Transformer 2 (Tra2). We find that the ribonucleoprotein consensus sequence (RNP-CS) of Tra2 is required for male fertility and positive and negative control of alternative splicing in transgenic f...
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PMID: 8124712
PDF is available here.
PDF is available here.
Abstract
We characterized the order by which the three introns are spliced from the primary transcript and processing intermediates to give rise to a mature mRNA and evaluated the potential role of gene transcription and pre-mRNA processing in the control of proGnRH mRNA levels in vivo. Nuclear and cytoplasm...
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PMID: 8307966
PDF is available here.
PDF is available here.
Nucleic Acids Research (22)3 1994
Abstract
We found that when the nascent RNA species could fold itself to form the rodlike structure, the HDV polyadenylation was suppressed 3 to 5 fold by the HDAg. In addition, we observed that the small and the large HDAg exerted a similar suppressive effect on the HDV polyadenylation, though they played d...
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PMID: 8127676
PDF is available here.
PDF is available here.
Abstract
EMBO Journal (12)13 1993
Abstract
We analyze the role of the conserved nucleotides at the first and penultimate positions (G1 and A886) of a group II self-splicing intron. We show that the G1 nucleotide is essential for the efficiency of both the first and the second splicing steps, while substitutions at the penultimate nucleotide...
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PMID: 8262060
PDF is available here.
PDF is available here.
EMBO Journal (12)13 1993
Abstract
We have analyzed base pairing interactions between the U5 snRNA and 5' exon sequences during pre-mRNA splicing in a mammalian in vivo system. We constructed synthetic U5 genes with mutations that alter four bases (C3, U4, U5 and U6) within the invariant 9 nt U5 sequence GCCUUUUAC; transient transfec...
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PMID: 8262061
PDF is available here.
PDF is available here.
EMBO Journal (12)13 1993
Abstract
The human U1A protein-U1A pre-mRNA complex and the relationship between its structure and function in inhibition of polyadenylation in vitro were investigated. Two molecules of U1A protein were shown to bind to a conserved region in the 3' untranslated region of U1A pre-mRNA. The secondary structure...
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PMID: 8262062
PDF is available here.
PDF is available here.
Biochemistry (Washington) (32)48 1993
Abstract
Truncated precursor tRNAs lacking the D arm or anticodon arm were studied in vitro as substrates for RNase P enzymes from Escherichia coli, Thermus thermophilus (eubacteria), and HeLa. Deletion of the D arm still allowed 5'-processing by E. coli RNase P, but strongly impaired maturation by T. thermo...
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PMID: 7694652
PDF is available here.
PDF is available here.