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DNA Transposable Elements (0):
Recent article
DNA Transposable Elements
Abstract
The dipteran Chironomus riparius is found across the entire Palearctic region; its larvae are among the most abundant macroinvertebrates inhabiting inland waterbodies. Chironomid larvae have been extensively used in ecotoxicological and cytogenetic research, but relatively little is...
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PMID: 21901555
PDF is available here.
PDF is available here.
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Abstract
We evaluated 12 DNA extraction methods and developed a highly sensitive protocol for mycobacterial DNA extraction directly from sputa using surface-coated magnetic particles. We have also developed a novel multiplex real-time PCR for simultaneous identification of M. tuberculosis complex and the Bei...
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PMID: 21593264
PDF is available here.
PDF is available here.
Hereditas (Beijing) (33)5 2011
Abstract
Transgenic technique provides a new way for fish breeding. Stable lines of growth hormone gene transfer carps, salmon and tilapia, as well as fluorescence protein gene transfer zebra fish and white cloud mountain minnow have been produced. The fast growth characteristic of GH gene transgenic fish wil...
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PMID: 21586396
PDF is available here.
PDF is available here.
Abstract
We provide the first genome sequence for S. agalactiae isolated from a cow diagnosed with clinical mastitis (strain FSL S3-026). Comparison to eight S. agalactiae genomes obtained from human disease isolates revealed 183 genes specific to the bovine strain. Subsequent polymerase chain reaction (PCR)...
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PMID: 21536150
PDF is available here.
PDF is available here.
Abstract
We describe a novel gene involved in adherence to epithelial cells. Transformation of C. diphtheriae 225, biotype gravis, ribotype St-Petersburg by EZ:TN(KAN-2)Tnp Transposome was undertaken. A C. diphtheriae 225 Tn5 insertion library of 2800 mutants was created. Five hundred and eighty five transfo...
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PMID: 21111065
PDF is available here.
PDF is available here.
Abstract
Epidemiological studies of the naturally transformable bacterial pathogen Streptococcus pneumoniae have previously been confounded by high rates of recombination. Sequencing 240 isolates of the PMEN1 (Spain(23F)-1) multidrug-resistant lineage enabled base substitutions to be distingu...
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PMID: 21273480
PDF is available here.
PDF is available here.
Abstract
We show that the Drosophila nucleolus contains CTCF, which is bound to transposable element sequences within the rDNA. Reduction in CTCF gene activity results in nucleolar fragmentation and reduced rDNA silencing, as does disruption of poly-ADP-ribosylation thought to be necessary for CTCF nucleolar...
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PMID: 21283722
PDF is available here.
PDF is available here.
American Surgeon (11) 2011
Abstract
Abstract
We explored the chromosomally-encoded determinants that contribute to the phenotype of susceptibility of P. aeruginosa to ceftazidime, imipenem and carbapenem. Using a comprehensive library of transposon-tagged insertion mutants, we found 37 loci in the chromosome of P. aeruginosa that contributed t...
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PMID: 21304266
PDF is available here.
PDF is available here.
Abstract
We speculate that intrastrain transposition of the three retroelements studied is controlled predominantly at the post-transcriptional level....
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PMID: 21779346
PDF is available here.
PDF is available here.
Abstract
We present a series of computer simulations on the genetic modification of disease vectors. We compared the effectiveness of two techniques of genetic modification, transposable elements and maternal effect dominant embryonic arrest (MEDEA). A gene drive mechanism based on MEDEA is introduced in the...
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PMID: 21431574
PDF is available here.
PDF is available here.
Methods in Cell Biology (104) 2011
Abstract
We describe both autonomous and non-autonomous transposable elements, and we compare and contrast popular transposon systems. The Tol2 system is a robust gene transfer tool and has been selected as the primary transposon platform, facilitating the development of an array of reagents readily shared w...
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PMID: 21924161
PDF is available here.
PDF is available here.
Abstract
We investigated the influence of minocycline ointment use on oral bacteria, using supragingival plaque samples from adults who had not taken any antibiotics for 6 months. Initially we investigated the effect of topical minocycline administration on the emergence of tetracycline-resistant oral bacter...
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PMID: 21519133
PDF is available here.
PDF is available here.
Abstract
We have developed a single-tube nested real-time PCR (STN-RT PCR) assay using the repetitive, transposon-like element IS1111 as the DNA target to facilitate early diagnosis of acute Q fever. The use of our proposed diagnostic procedures, including IgM detection by serology and the STN-RT PCR assay,...
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PMID: 21519134
PDF is available here.
PDF is available here.
Abstract
Cytogenetic and Genome Research (132)1-2 2011
Abstract
We aimed to characterize TEs to help elucidate their impact on the genomic architecture, diversity and evolution of chromosomes in the D. buzzatii cluster of species (repleta group).
A full TE element of D. buzzatii, named PERI, was identified in a BAC clone available in GenBank. PERI was further an...
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PMID: 20938165
PDF is available here.
PDF is available here.
Abstract
Epigenetic modifications of the genome are generally stable in somatic cells of multicellular organisms. In germ cells and early embryos, however, epigenetic reprogramming occurs on a genome-wide scale, which includes demethylation of DNA and remodeling of histones and their modifications. The mecha...
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PMID: 21030646
PDF is available here.
PDF is available here.
Cold Spring Harbor Protocols (2010)11 2010
Abstract
RNA interference (RNAi) is an evolutionarily conserved process that silences gene expression through double-stranded RNA species in a sequence-specific manner. Small interfering RNAs (siRNAs) can promote sequence-specific degradation and/or translational repression of target RNA by a...
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PMID: 21041394
PDF is available here.
PDF is available here.
EMBO Journal (29)20 2010
Abstract
We show in Arabidopsis that the exclusion depends on a histone demethylase gene, IBM1 (increase in BONSAI methylation). Loss-of-function ibm1 mutation results in ectopic H3K9me and non-CG methylation in thousands of genes. The ibm1-induced genic H3K9me depends on both histone methylase KYP/SUVH4 and...
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PMID: 20834229
PDF is available here.
PDF is available here.
Abstract
We report a clinical isolate of Enterococcus faecium in which three distinct and excision-proficient copies of Tn916-like elements are present in the genome. All of the elements contain tet(M) genes, at least one of which confers resistance to tetracycline and minocycline. Two elements (Tn6085a, Tn6...
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PMID: 20600284
PDF is available here.
PDF is available here.
Abstract
While morphology, immunophenotyping, cytogenetics, and clinical history continue to play an important role, an increasing number of molecular tests are now required to properly classify these cases....
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PMID: 20923295
PDF is available here.
PDF is available here.
Molecular Therapy (18)10 2010
Abstract
We compared the genomic integration efficiencies and transposition site preferences of Sleeping Beauty (SB or SB11), Tol2, and piggyBac (PB) transposon systems in primary T cells derived from peripheral blood lymphocytes (PBL) and umbilical cord blood (UCB). We found that PB demonstrated the highest...
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PMID: 20606646
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
After the radiation of primates and rodents, the evolution of X-chromosome inactivation centers in human and mouse (XIC/Xic) followed two different directions. Human XIC followed the pathway towards transposon accumulation (the repeat proportion in the center constitutes 72%), especially LINEs, whic...
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PMID: 21254562
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
The functional organization of particular chromosome regions is tightly associated with their function in eukaryotic cells. Details of this association are among the most topical problems of modem genetics. The paper characterizes the results of recent research of the specifics of the genetic organi...
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PMID: 21254569
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
The Xist gene belongs to the class of long noncoding regulatory RNA genes which play a key role in the process of inactivation of one of the X chromosomes in females of placental mammals. Based on interspecific comparative sequence analysis performed using a set ofbioinformatic programs and approach...
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PMID: 21254561
PDF is available here.
PDF is available here.
Abstract
Our results indicate that PCR amplification and sequencing of the mycobacterial hsp65 gene is a sensitive assay for identification of NTM species in FFPE materials. However, consistent results of PCR analysis, microbiology study, histologic manifestations, radiology, and clinical presentation are im...
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PMID: 20850247
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)10 2010
Abstract
We performed an 8-year retrospective study of all hospitalized patients with recurrent VRE colonization after they were documented as being clear of VRE and compared the primary colonization isolates and recolonization isolates by pulsed-field gel electrophoresis and Tn1546 typing. Review of the med...
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PMID: 20109265
PDF is available here.
PDF is available here.
Abstract
We investigated 16 blaKPC-2-harboring K. pneumoniae isolates from 5 countries. The isolates were multidrug resistant, possessed the blaKPC-2 gene, and differed by additional Beta-lactamase content. They harbored a naturally chromosome-encoded bla gene (blaSHV-1 [12.5%], blaSHV-11 [68.7%], or blaOKP-...
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PMID: 20735917
PDF is available here.
PDF is available here.
Abstract
A microsphere-based multiplex assay was developed, by using the xMAP technology, for the simultaneous rapid detection of the Mycobacterium tuberculosis complex (MTC) and the differentiation of M. tuberculosis and M. bovis. The assay simultaneously detected 4 target sequences, including specific inse...
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PMID: 20736748
PDF is available here.
PDF is available here.
Abstract
We investigate the role of recombination in transposable element (TE) proliferation in the cyclical parthenogen, Daphnia pulex. Recombination provides a mechanism by which the rate of both TE gain and loss can be accelerated, a duality that has long intrigued many biologists interested in the influe...
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PMID: 20356890
PDF is available here.
PDF is available here.
Abstract
We describe a link between transposition of IS608 and ISDra2, both members of the IS200/IS605 family, which uses obligatory single-stranded DNA intermediates, and the host replication fork. Replication direction through the IS plays a crucial role in excision: activity is maximal when the "top" IS s...
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PMID: 20691900
PDF is available here.
PDF is available here.
Abstract
We explore the consequences of genomic merger and doubling on copia and gypsy-like Gorge3 long terminal repeat (LTR) retrotransposons as well as on non-LTR long interspersed nuclear elements (LINEs) in allotetraploid cotton, Gossypium hirsutum. Using phylogenetic and quantitative methods, we describ...
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PMID: 20725147
PDF is available here.
PDF is available here.
Abstract
We found a novel piggyBac-like DNA transposon that potentially encodes an intact transposase (610 amino acid residues), which is flanked by 16-bp perfect inverted terminal repeats and a duplicated TTAA target site. Interestingly, we also identified another intact copy of this transposon on an autoso...
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PMID: 20725145
PDF is available here.
PDF is available here.
Journal of Virology (84)16 2010
Abstract
We performed random mutagenesis of PrV pUL36 by transposon-mediated insertion of a 15-bp linker. By this approach, 26 pUL36 insertion mutants were selected and tested in transient transfection assays for their ability to complement one-step growth and/or viral spread of a PrV UL36 null mutant. Ten i...
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PMID: 20534865
PDF is available here.
PDF is available here.
Nature Reviews: Genetics (11)8 2010
Abstract
Most of the human genome consists of non-protein-coding DNA. Recently, progress has been made in annotating these non-coding regions through the interpretation of functional genomics experiments and comparative sequence analysis. One can conceptualize functional genomics analysis as involving a sequ...
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PMID: 20628352
PDF is available here.
PDF is available here.
Abstract
A collection of 4117 fertile T-DNA lines has been generated by Agrobacterium-mediated transformation of the diploid community standard line Bd21 of Brachypodium distachyon. The regions flanking the T-DNA left and right borders of the first 741 transformed plants were isolated by adap...
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PMID: 20374523
PDF is available here.
PDF is available here.
Nucleic Acids Research (38)14 2010
Abstract
We have created a universal, sequence-verified TagModule collection. A hallmark of the 4280 TagModules is that they are cloned into a Gateway entry vector, thus facilitating rapid transfer to any compatible genetic system. Here, we describe the application of the TagModules to rapidly generate tagge...
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PMID: 20494978
PDF is available here.
PDF is available here.
Cancer Letters (294)1 2010
Abstract
We report that in glioblastoma, EGFR/EGFRvIII paradoxically co-expresses with p53-upregulated modulator of apoptosis (PUMA), a proapoptotic member of the Bcl-2 family of proteins primarily located on the mitochondria. EGFR/EGFRvIII binds to PUMA constitutively and under apoptotic stress, and subsequ...
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PMID: 20153921
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (309)1 2010
Abstract
Tn6000 (formerly EfcTn1) from Enterococcus casseliflavus strain 664.1H1 (previously Enterococcus faecium 664.1H1) is a tetracycline resistance-encoding conjugative transposon of the Tn916-like family of mobile genetic elements. Sequence analysis of Tn6000 shows that it has a novel mo...
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PMID: 20528943
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (309)1 2010
Abstract
The Lancefield group C alpha-hemolytic Streptococcus dysgalactiae ssp. dysgalactiae (GCSD) causes systemic granulomatous inflammatory disease and high mortality rates in infected fish. Superantigen and streptolysin S genes are the most important virulence factors contributing to an i...
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PMID: 20528946
PDF is available here.
PDF is available here.
Infection and Immunity (78)8 2010
Abstract
We have identified several loci that are required for C. jejuni efficient entry and survival within epithelial cells. Among these loci, insertional mutations in aspA, aspB, and sodB resulted in drastic reduction in C. jejuni entry and/or survival within host cells and a severe defect in colonization...
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PMID: 20515930
PDF is available here.
PDF is available here.
Genetics Research (92)4 2010
Abstract
TP5, a P element inserted in the telomere-associated sequences of the X chromosome, represses the excision of other P elements in the germ line through a combination of maternal and zygotic effects. The maternal component of this repression is impaired by heterozygous mutations in the aubergine and...
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PMID: 20943007
PDF is available here.
PDF is available here.
Molecules and Cells (30)1 2010
Abstract
We investigated the number of transposable element (TE)-derived dual coding genes in human, chimpanzee and mouse genomes. TE fusion exons appeared in the dual coding regions of 309 human genes. Functional protein domain alterations by TE-derived dual coding events were observed in 129 human genes. C...
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PMID: 20652499
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
This review summarizes older as well as recent data about the model dioecious plant Silene latifolia. This plant has been the subject of more than one hundred years of research efforts and its most conspicuous property is huge and well differentiated heteromorphic sex chromosomes, XX in females and...
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PMID: 20551610
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
We have examined chloroplast DNA insertions in maize chromosomes using fluorescence in situ hybridization (FISH). Probes to detect the nuclear-plastid sequences (NUPTs) were generated using 14 overlapping fragments of the chloroplast genome. Using current FISH methods, detection of NUPTs on mitotic...
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PMID: 20628248
PDF is available here.
PDF is available here.
Trends in Parasitology (26)7 2010
Abstract
Transposable elements (TEs) are dynamic elements that can reshape host genomes by generating rearrangements with the potential to create or disrupt genes, to shuffle existing genes, and to modulate their patterns of expression. In the genomes of parasites that infect mammals several TEs have been id...
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PMID: 20444649
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
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PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
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PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
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PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
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PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
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PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
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PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
|
PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
|
PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
|
PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
|
PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
|
PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
|
PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
|
PMID: 20409008
PDF is available here.
PDF is available here.
Plant Journal (63)1 2010
Abstract
We describe a new method that can substantially increase the throughput of linking a disrupted gene to a known phenotype in high-copy Mutator (Mu) transposon lines in maize. The approach uses the Illumina platform to obtain sequences flanking Mu elements in pooled, bar-coded DNA samples. Insertion s...
|
PMID: 20409008
PDF is available here.
PDF is available here.