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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
DNA, Antisense (1):
Recent article
DNA, Antisense
Abstract
We have investigated the evolution of myocardial leptin expression following myocardial infarction (MI) and evaluated the consequences of specific cardiac leptin inhibition on subsequent LV dysfunction. Anaesthetized rats were subjected to temporary coronary occlusion. An antisense oligodesoxynucleo...
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PMID: 20731748
PDF is available here.
PDF is available here.
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Anticancer Research (30)9 2010
Abstract
Immunohistochemical investigation demonstrated increased expression of SMAD2, -3 and -4, and decreased expression of SMURF2. TGF-β1 antisense therapy significantly down-regulated SMAD2 and SMAD4, up-regulated SMURF2 and showed no effect on SMAD3 and SMAD6. CONCLUSION: TGF-β1 led to elevated levels...
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PMID: 20944123
PDF is available here.
PDF is available here.
Abstract
We characterize the expression of a KLK4 cis sense-antisense chimeric transcript, and show other examples in prostate cancer. Using non-protein-coding microarray analyses, we initially identified an androgen-regulated antisense transcript within the 3' untranslated region of the KLK4 gene in LNCaP c...
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PMID: 20406994
PDF is available here.
PDF is available here.
Biophysical Journal (98)8 2010
Abstract
We developed a single-molecule tethered particle motion (TPM) method to investigate the unfolding process of G4s in the human telomeric sequence AGGG(TTAGGG)3 in real time. The TPM method monitors the DNA tether length change caused by formation of the G4, thus allowing the unfolding process and str...
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PMID: 20409481
PDF is available here.
PDF is available here.
Abstract
The developmental changes in the hepatic cytochrome P450 (CYP) content, mRNA expression of 12 hepatic CYP subtypes, and the enzyme activities of 5 hepatic CYP subfamilies in rats were investigated using non-treated male and female Sprague-Dawley rats of ages postnatal day (PD) 4, 16, 30 and 8 and 12...
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PMID: 20032627
PDF is available here.
PDF is available here.
Cell Research (20)4 2010
Abstract
We found that a protein encoded by the antisense gene ADF1 acts as a transcription suppressor, regulating the expression of sense gene MDF1 in Saccharomyces cerevisiae. Based on the evolutionary, genetic, cytological and biochemical evidence, we show that the protein-coding sense gene MDF1 most like...
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PMID: 20195295
PDF is available here.
PDF is available here.
Plant Physiology (152)3 2010
Abstract
The alternative oxidase (AOX) is a cyanide-resistant oxidase that provides an alternative outlet for electrons from the respiratory electron transport chain embedded in the inner membrane of plant mitochondria. Examination of soybean (Glycine max) plants carrying a GmAOX2b antisense gene showed AOX...
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PMID: 20097793
PDF is available here.
PDF is available here.
Abstract
Chitosan/alginate nanoparticles which had been optimized in our previous study using two different N/P ratios were chosen and their ability to release epidermal growth factor receptor (EGFR) antisense was investigated. In addition, the stability of these nanoparticles in aqueous medium and after fre...
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PMID: 20957167
PDF is available here.
PDF is available here.
Abstract
To gain a deeper understanding of the physicochemical phenomenon of self-assembled nanoparticles of different generations and ratios of poly (amidoamine) dendrimer (PAMAM) dendrimer and a short-stranded DNA (antisense oligonucleotide), multiple methods were used to characterize these nanoparticles i...
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PMID: 20517481
PDF is available here.
PDF is available here.
Cancer Investigation (27)10 2009
Abstract
We have investigated the correlation between hARD1 and breast cancer. Analysis with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry (FCM) demonstrated that overexpression of hARD1 was associated with increased proliferation of MCF-7 cell, a human breast cancer c...
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PMID: 19909012
PDF is available here.
PDF is available here.
Journal of Immunology (183)3 2009
Abstract
We now show that switching/sucrose nonfermenting (SWI/SNF) chromatin remodeling complexes are recruited to the Igh locus by an enhancer-dependent process and that these complexes are essential for generating recombinase accessibility throughout the locus. Depletion of SWI/SNF in pro-B cells also inh...
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PMID: 19596997
PDF is available here.
PDF is available here.
Cancer Research (69)15 2009
Abstract
We have shown for the first time that Drg1 directly binds to the BH3-only proapoptotic protein Bim. Depletion of Drg1 by small interfering RNA induced up-regulation of Bim and its accumulation in the mitochondria, which correlated with loss of mitochondrial membrane potential and induction of apopto...
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PMID: 19622774
PDF is available here.
PDF is available here.
Abstract
Chemiluminescence detection of reactive oxygen species (ROS) triggered in tobacco BY-2 cells by the fungal elicitor cryptogein was previously demonstrated to be abolished in cells transformed with an antisense construct of the plasma membrane NADPH oxidase, NtrbohD. Here, using electron microscopy,...
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PMID: 19522569
PDF is available here.
PDF is available here.
Gene Therapy (16)7 2009
Abstract
We examined the changes in ERCC1 expression, as well as the functional significance of ERCC1 in the rat brain following middle cerebral artery occlusion (MCAO). The results were as follows: (1) ERCC1 immunopositive cells were widely distributed in various brain regions. ERCC1 expression was localize...
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PMID: 19440222
PDF is available here.
PDF is available here.
Abstract
We constructed cationic liposome loaded with the proapoptotic peptide, d-(KLAKLAK)(2) and the Bcl-2 antisense oligodeoxynucleotide, G3139, and determined whether the combination of the proapoptotic macromolecules in a single cationic liposome can enhance antitumor efficacy. Advantage was taken of al...
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PMID: 19317442
PDF is available here.
PDF is available here.
Journal of Antibiotics (62)5 2009
Abstract
Treatment of drug-resistant bacteria is a significant unmet medical need. This challenge can be met only by the discovery and development of new antibiotics. Antisense technology is one of the newest discovery tools that provides enhanced sensitivity for detection of antibacterials, and has led to t...
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PMID: 19343064
PDF is available here.
PDF is available here.
Abstract
Comparing with the blank group, the proliferation inhibition rate of the Sw626 and A2780 cells transfected with anti-sense PCDGF was 72.9% and 70.9%, respectively, and the invasion ability was inhibited by 62.9% and 59.0%, respectively. The levels of cyclin D1 and CDK4 protein expression in antisens...
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PMID: 19538881
PDF is available here.
PDF is available here.
Abstract
In the present work, inductively coupled plasma atomic emission spectrometry (ICP-AES) was used to determine the content of Zn, Na and K and the ratio of Na/K in antisense ACS transgenic tomato fruit (in which the antisense ACC synthase gene construct was inserted) and in normal tomato fruit of the...
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PMID: 19445235
PDF is available here.
PDF is available here.
Abstract
We review the current understanding of the extent, functions and significance of antisense transcription. We critically discuss results from genome-wide studies and documented examples of individual antisense transcripts. So far, the regulatory potential of gene overlaps has been demonstrated only i...
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PMID: 18791843
PDF is available here.
PDF is available here.
Abstract
Over expression of pkv in Nicotiana tabacum cv. Xanthi (tobacco) resulted in stunting, reduced root formation, and delay in flowering, phenotypes similar to symptoms of PSTVd infection of tomato. In addition, homozygous T2 tobacco plants over expressing PKV were male sterile. Antisense expression of...
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PMID: 19689802
PDF is available here.
PDF is available here.
BMC Genomics (10)1 2009
Abstract
On a spontaneous sweet orange bud mutant with abnormal lycopene accumulation in fruits and its wild type, comparative transcripts profiling was performed using Massively Parallel Signature Sequencing (MPSS). A total of 6,877,027 and 6,275,309 reliable signatures were obtained for the wild type (WT)...
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PMID: 19922663
PDF is available here.
PDF is available here.
Abstract
Custirsen is well tolerated in humans and biologically active in inhibiting expression of clusterin in patients with cancer. Randomized trials of custirsen in combination with chemotherapy are planned in patients with castration-resistant prostate cancer....
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PMID: 19012510
PDF is available here.
PDF is available here.
Antiviral Research (80)3 2008
Abstract
We first established that sequence and translational activity of HCV IRESs cloned from six patients, whether responders or not to combination therapy, were conserved. We then tested the hypothesis that antisense molecules, i.e. short peptide nucleic acids (PNA), could inhibit HCV translation by bind...
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PMID: 18625270
PDF is available here.
PDF is available here.
Journal of Molecular Biology (383)5 2008
Abstract
We show that the silkmoth chromo-helicase/ATPase-DNA binding protein 1 (CHD1) ortholog is responsible for repositioning of nucleosomes on chorion promoters, where the factor binds specifically. Chorion genes, occupying a single chromosomal locus, rely on an almost identical set of cis elements for t...
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PMID: 18817785
PDF is available here.
PDF is available here.
Abstract
We present evidence that the open reading frames on the antisense strand of HSP70 genes are spurious, and we identify a more probable candidate for the gene encoding the oomycete NAD-GDH enzyme. These results cast extensive doubt on the Rosetta Stone argument....
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PMID: 19037009
PDF is available here.
PDF is available here.
Human T-cell leukemia virus type-1 antisense-encoded gene, Hbz, promotes T-lymphocyte proliferation.
Abstract
Our data indicate that Hbz expression enhances the proliferative capacity of HTLV-1-infected T cells, playing a critical role in cell survival and ultimately HTLV-1 tumorigenesis in the infected host....
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PMID: 18689544
PDF is available here.
PDF is available here.
Abstract
We hypothesized that coupling these two treatments would result in increased killing of prostate cancer cells. In this study, the effect of Ad-E2F1 and AS-MDM2 in combination with radiation was investigated in three prostate cancer cell lines: LNCaP cells, LNCaP-Res cells [androgen insensitive with...
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PMID: 19010821
PDF is available here.
PDF is available here.
Cancer Investigation (26)8 2008
Abstract
Our results suggested that antisense Ki-67 cDNA vector treatment might be an important potential option in the anticancer therapy....
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PMID: 18798062
PDF is available here.
PDF is available here.
Abstract
We prepared the SPIO-labeled ASODN probe successfully. It can effectively transfect SK-Br3 oncocyte and enter SK-Br3 oncocyte, and thus reduce the signal intension in MRI....
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PMID: 19024385
PDF is available here.
PDF is available here.
Journal of Biochemistry (144)4 2008
Abstract
These results suggest that the sense ssDNA fragments are useful for the correction of mutated genes. The variation in the correction efficiency may depend on the sequence of the target position in double-stranded DNA....
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PMID: 18586771
PDF is available here.
PDF is available here.
Human Molecular Genetics (17)18 2008
Abstract
We analyze microindels in human cancers using an endogenous and transcribed gene, the TP53 gene. Microindel frequency, the enhancement of 1-2 microindels and other features are generally similar to that observed in the non-transcribed lacI gene in normal mouse tissues. The current larger sample of s...
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PMID: 18632684
PDF is available here.
PDF is available here.
Abstract
PNA/DNA interstrand cross-links (ICLs) were observed when peptide nucleic acids (PNAs) containing modified thymine derivatives were hybridized with the complementary or one-base mismatched DNA upon photolysis or treatments of oxidative agent. PNA/DNA ICL formation provides a useful method for biolog...
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PMID: 18715781
PDF is available here.
PDF is available here.
Abstract
We use high-throughput sequencing to analyze Argonaute-associated small RNAs (sRNAs) in both the presence and absence of Cid14. Most sRNAs in fission yeast start with a 5' uracil, and we argue these are loaded most efficiently into Argonaute. In wild-type cells most sRNAs match to repeated regions o...
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PMID: 18776903
PDF is available here.
PDF is available here.
Analytical Biochemistry (380)1 2008
Abstract
We found that by increasing the probe surface density from 10(11) to 10(12) molecules/cm(2), the hybridization rate and efficiency are suppressed while the melting temperature of the DNA duplex increases. The maximum fraction of hybridized capture probes at equilibrium did not exceed 50% for hybridi...
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PMID: 18555787
PDF is available here.
PDF is available here.
Cancer Letters (267)1 2008
Abstract
We found that the expression of beta1,6-linked GlcNAc-bearing N-glycans on cell surface protein was gradually decreased after induction of apoptosis by As2O3-treatment. And, As2O3 significantly decreased the protein expression level of beta1,4GalT V, which effectively galactosylates the beta1,6-GlcN...
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PMID: 18439752
PDF is available here.
PDF is available here.
Chinese Medical Journal (121)15 2008
Abstract
We found the expression of the mRNA and protein levels of CLB1 decrease in these transfectants. The inhibition of CLB1 caused prominent G1 arrest, abnormal morphology, retarded cell growth and an increase in apoptosis. In AS-mCLB1-expressing LL/2 and CT-26 transfectants implanted mice, tumorigenicit...
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PMID: 18959122
PDF is available here.
PDF is available here.
Abstract
We generated transgenic zebrafish with cardiac-specific expression of CBD1 linked to yellow and cyan fluorescent protein. Ca(2+) binding to CBD1 induces conformational changes, as detected by fluorescence resonance energy transfer. With this transgenic fish model, we were able to monitor conformatio...
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PMID: 18550703
PDF is available here.
PDF is available here.
Abstract
We developed 18F-fluorodeoxygalactose(18F-FDGal), 18F-SAV03M, and 18F-FRP170 as tracers of the second category. 18F-FDGal is metabolized and trapped in the tissue by liver-specific galactose metabolic enzymes. It is used for detecting well-differentiated hepatocellular carcinoma which originated fro...
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PMID: 18701838
PDF is available here.
PDF is available here.
Molecular Cell (31)2 2008
Abstract
We show that ectopic expression of a long hairpin RNA bypasses the requirement for chromatin-dependent steps in siRNA generation. The ability of hairpin-produced siRNAs to silence homologous sequences in trans is subject to local chromatin structure, requires HP1, and correlates with antisense trans...
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PMID: 18657501
PDF is available here.
PDF is available here.
Abstract
CHD5 is the strongest candidate tumor suppressor gene that is deleted from 1p36.31 in neuroblastomas, and inactivation of the second allele may occur by an epigenetic mechanism....
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PMID: 18577749
PDF is available here.
PDF is available here.
Plant Cell Reports (27)7 2008
Abstract
Mitosis and cell wall synthesis in the legume root cap meristem can be induced and synchronized by the nondestructive removal of border cells from the cap periphery. Newly synthesized cells can be examined microscopically as they differentiate progressively during cap development, and ultimately det...
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PMID: 18347802
PDF is available here.
PDF is available here.
Transgenic Research (17)3 2008
Abstract
We show for the first time that changes in lignin content and composition can be achieved in a conifer by taking a transgenic approach. Lignin content and composition have been altered in five-year-old transgenic plants of Norway spruce (Picea abies [L.] Karst) expressing the Norway spruce gene enco...
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PMID: 17610137
PDF is available here.
PDF is available here.
Microvascular Research (76)1 2008
Abstract
Constitutive activation of the HIF-1 alpha C-TAD, and not merely stabilization of the HIF-1 alpha molecule, is essential for optimal HIF-mediated transcriptional and angiogenic effects. This finding could have important implications for therapeutic angiogenesis using HIF-1 alpha....
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PMID: 18440561
PDF is available here.
PDF is available here.
Fertility and Sterility (89)5 Suppl 2008
Abstract
I collagen, type III collagen, fibronectin, and transforming growth factor-beta (TGF-beta1) in fibroblasts obtained from normal peritoneal and adhesion tissues. DESIGN: Prospective experimental study. SETTING: University medical center. PATIENT(S): Fibroblasts established from peritoneal and adhesio...
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PMID: 17624339
PDF is available here.
PDF is available here.
Abstract
Cell models (TwistS-MKN28) that steadily expressed high Twist protein were obtained. Compared with MKN28 and pcDNA3-MKN28 cells, adherence, migration and invasion ability of TwistS-MKN28 cells were clearly raised. The number of cancer nodules was increased significantly in the abdominal cavity and l...
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PMID: 18442194
PDF is available here.
PDF is available here.
Medicina Clínica (130)13 2008
Abstract
Difficulties derived from the big complexity of the human genome (because of the great amount of genes) and difficulties at the time of performing a correct molecular diagnosis have led to the utilization of advanced technologies such as DNA arrays. By this technique, it is possible to analyze in a...
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PMID: 18423170
PDF is available here.
PDF is available here.
Abstract
Intranasal administration of gd21 prevented airway inflammation and mucus production and inhibited development of airway hyperresponsiveness to methacholine in models of acute allergic airway inflammation. Similar effects were also detected in a model of chronic experimental asthma. Interestingly, g...
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PMID: 18325571
PDF is available here.
PDF is available here.
Abstract
We have designed and performed a large-scale antisense-based genetic screen in primary mouse embryo fibroblasts (MEFs). Out of this screen, we have identified five different genes through which loss of function partially bypasses senescence. These genes belong to very different biochemical families:...
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PMID: 17968325
PDF is available here.
PDF is available here.
Abstract
Abnormal wound healing processes can result in hypertrophic scars and keloids. Transforming growth factor-beta1 (TGF-beta1) and hepatocyte growth factor/scatter factor (HGF/SF) are biphasic growth factor cytokines in physiologic and pathophysiologic conditions. Findings have shown TGF-beta1 to be pi...
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PMID: 18087663
PDF is available here.
PDF is available here.
Abstract
This study is to investigate the expression of CyclinD1 in asthmatic rats and construct expression plasmids of sense and antisense CyclinD1 gene and transfect them to asthmatic airway smooth muscle cell to study the effects of CyclinD1 on the proliferation of airway smooth muscle cells in asthmatic...
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PMID: 18630259
PDF is available here.
PDF is available here.
Abstract
The interaction with DNA of a peptide nucleic acid (PNA) oligomer (16nt) conjugated with a nuclear localization signal (NLS) peptide, which was previously found to be able to inhibit tumor cell proliferation through block of transcription of the MYCN oncogene, was studied by UV and CD spectroscopy....
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PMID: 17963203
PDF is available here.
PDF is available here.
Abstract
We generated transgenic tobacco plants in which the amount of endogenous rbcS mRNA can be reduced by inducible expression of antisense rbcS mRNA with dexamethasone (DEX) treatment at later stages of growth. In already expanded leaves, after DEX treatment, the level of photosynthesis, RuBisCO quantit...
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PMID: 18178584
PDF is available here.
PDF is available here.
Abstract
We found that PKC alpha was highly expressed in the poor-differentiated HCC cell lines (SK-Hep-1 and HA22T/VGH) as compared with that in the well-differentiated HCC cell lines (PLC/PRF/5, Hep3B, and HepG2). When treated with PKC alpha antisense oligonucleotides (ODN), both HA22T/VGH and SK-Hep-1 cel...
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PMID: 17486587
PDF is available here.
PDF is available here.
Abstract
Culture of human RS cells with pancreatic cancer KP-4 cell-conditioned medium resulted in increased UV mutagenicity, possibly via the down-regulation of GRP78/BiP....
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PMID: 18192872
PDF is available here.
PDF is available here.
Development (135)2 2008
Abstract
We recently demonstrated that Tsix silences Xist through modification of the chromatin structure in the Xist promoter region. This finding prompted us to investigate the role of antisense transcription across the Xist promoter in Tsix-mediated silencing. Here, we prematurely terminated Tsix transcri...
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PMID: 18057104
PDF is available here.
PDF is available here.
Abstract
The protein level of vimentin was higher in PC-3M-1E8 cells with high metastatic potential than in PC-3M-2B4 cells with low metastatic potential. Eukaryotic vectors expressing antisense-and sense-vimentin were constructed successfully and transfected into PC-3M-1E8 and PC-3M-2B4 cells separately. Th...
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PMID: 18184460
PDF is available here.
PDF is available here.
Abstract
We focus on drug development using ASO and siRNA strategies to inhibit picornavirus infections. The picornavirus genome organization and life cycle is described, followed by discussion of design considerations, chemical modifications and drug delivery approaches. Recent studies using antisense again...
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PMID: 18508540
PDF is available here.
PDF is available here.
Abstract
Homologous recombination is the most precise way to manipulate the genome. As a tool it has been used extensively in bacteria, yeast, murine embryonic stem cells, and a few other specialized cell lines but has not been available to researchers in other systems, such as for mammalian somatic cell gen...
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PMID: 18370067
PDF is available here.
PDF is available here.
Abstract
These findings indicate that CTGF is involved in ECM production in SCFs activated by exogenous TGFbeta1 in vitro. Inhibition of CTGF expression may be effective in preventing undesirable scar formation during healing following filtration surgery....
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PMID: 18369694
PDF is available here.
PDF is available here.
Plant Molecular Biology (65)6 2007
Abstract
We investigate the relationships between systemin and the tomato HypSys peptides in regulating wound signaling in tomato plants. Analysis of transgenic tomato plants over-expressing sense and antisense constructs of the tomato HypSys precursor under the 35S CaMV promoter show that the transgenic pla...
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PMID: 17899396
PDF is available here.
PDF is available here.