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DNA, Catalytic (0):
Recent article
DNA, Catalytic
Abstract
An efficient pyrene-assisted method has been developed for the photolysis of disulfide bonds, with 77% of disulfides cleaved after only 20 min of irradiation (0.3W) at 350 nm. By employing a DNA framework, it was possible to observe both a distance-dependent cleavage pathway and a ra...
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PMID: 21080636
PDF is available here.
PDF is available here.
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Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology (18)8 2010
Abstract
Nanotechnology (21)20 2010
Abstract
Using unimolecular copper(II)-dependent self-cleaving deoxyribozymes (DNAzymes), a label-free colorimetric biosensor for copper(II) ions (Cu2+) has been developed based on the sequence-length-dependent adsorption of single-stranded deoxyribonucleic acid (ssDNA) on unmodified gold nanoparticles (AuNP...
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PMID: 20418604
PDF is available here.
PDF is available here.
Chemical Communications (46)18 2010
Abstract
A novel amplification strategy based on DNAzyme functionalized gold nanoparticles was employed to enhance the sensitivity of an electrochemical sensor for detection of Pb2+. The detection limit is 0.028 nM, which is much lower than for other electrochemical DNAzyme sensors.
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PMID: 20361096
PDF is available here.
PDF is available here.
Abstract
We have previously reported the photochemical activation of DNAzymes and antisense agents through the preparation of a caged DNA phosphoramidite and its site-specific incorporation into oligonucleotides. The presence of the caging group disrupts either DNA:RNA hybridization or catalytic activity unt...
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PMID: 20392038
PDF is available here.
PDF is available here.
Abstract
We investigate the construction of a novel ssDNA expression vector in this study. In order to improve the expression efficiency, the mmlv-rt gene and ODN-PMT (an oligodeoxynucleotide including other essential sequences for generating ssDNA) were cloned into a single plasmid under the control of 2 se...
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PMID: 20059315
PDF is available here.
PDF is available here.
Abstract
The oligonucleotide Dz13, a DNA enzyme that cleaves c-Jun mRNA, is capable of inhibiting various model tumors in mice. However, to date, a thorough examination of its target specificity in tumor cells has not been performed. In addition, an evaluation of its safety in a mammalian whole organism syst...
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PMID: 20148692
PDF is available here.
PDF is available here.
Abstract
We here show that when a panel of human tumor cells that naturally propagate in bone are challenged with Dz13, the tumor suppressor E2F1 is upregulated regardless of cellular p53 status. The piddosomal components, p53-induced protein with a death domain and caspase-2, were translocated to the nucleu...
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PMID: 20406096
PDF is available here.
PDF is available here.
Chemical Communications (46)13 2010
Abstract
New deoxyribozymes are shown to catalyze reactions of serine side chains, forming nucleopeptide linkages and discriminating between serine and tyrosine or between two competing serines.
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PMID: 20234910
PDF is available here.
PDF is available here.
Chemical Communications (46)8 2010
Abstract
The activities of Mg(2+)-dependent DNAzymes are reversibly switched by pH stimuli using the i-motif as an activating motif.
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PMID: 20449252
PDF is available here.
PDF is available here.
Abstract
The aim of this review is to provide the reader with an overview on the potential of dendritic polymers in the antisense delivery technology. Special emphasis has been placed on the different types of dendritic structures that have been reported and the modifications performed to increase their effi...
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PMID: 20380642
PDF is available here.
PDF is available here.
Abstract
The discovery of catalytic nucleic acids (CNAs) has provided scientists with valuable tools for the identification of new therapies for several untreated diseases through down regulation or modulation of endogenous gene expression involved in these ailments. These CNAs aim either towards the elimina...
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PMID: 20714308
PDF is available here.
PDF is available here.
Abstract
Rapid increase in drug-resistant influenza virus isolates, and pandemic threat posed by highly pathogenic avian influenza A and swine flu viruses provide clear and compelling reasons for fast tracking development of novel antiviral drugs. Nucleic acid-based drugs represent a promisin...
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PMID: 20036985
PDF is available here.
PDF is available here.
Abstract
The peroxidase activities of the complexes of hemin and intermolecular four-stranded G-quadruplexes formed by short-stranded X(n)G(m)X(p) sequences (X=A, T or C), especially T(n)G(m)T(p) sequences, were compared. The results, combining with those of circular dichroism (CD) spectra and acid-base tran...
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PMID: 19836504
PDF is available here.
PDF is available here.
Zhonghua Yixue Zazhi (89)38 2009
Abstract
A novel expression vector capable of generating specific 10-23DZ in cells has been successfully constructed. And the intracellular product 10-23DZ may inhibit the expression of any target gene of interest....
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PMID: 20137274
PDF is available here.
PDF is available here.
Abstract
We introduced diverse chemical modifications within Dz1023-434 catalytic core to produce a stable locked nucleic acid (LNA)-modified DXZ (Dz434-LNA) with significant cleavage activity of full E6/E7 transcripts. Cell culture testing of Dz434-LNA produced a sharp decrement of E6/E7 mRNA levels in HPV-...
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PMID: 19732021
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)31 2009
Abstract
We seek to deduce the properties and sequence requirements of this enzyme. This deoxyribozyme was found to cleave a 23-nt chimeric DNA-RNA substrate, which contains a single ribonucleotide flanked by fluorophore- and quencher-modified nucleotides on each side of the cleavage junction. Extensive nucl...
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PMID: 19583262
PDF is available here.
PDF is available here.
Oncology Reports (22)1 2009
Abstract
We found it strongly inhibited MMP-9 mRNA and protein expression in the NSCLC cell line A549. Inhibition of cell proliferation, adhesion, migration and invasion was also demonstrated. Thus, DNAzyme targeting MMP-9 may be a promising anti-invasion and anti-metastasis strategy for cancer gene therapy...
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PMID: 19513513
PDF is available here.
PDF is available here.
Abstract
Our increasing understanding of the regulatory mechanisms involved in the pathogenesis of disease is opening up opportunities for therapeutic intervention. To tackle the unmet disease burden, the last decade has seen the emergence of gene-targeting small-molecule nucleic acid-based strategies, such...
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PMID: 19402864
PDF is available here.
PDF is available here.
ChemBioChem (10)3 2009
Abstract
Uranyl ion-specific DNAzyme: A DNAzyme (lower strand) cleaves the substrate (upper strand) in the presence of the uranyl ion. The enzyme folds into a bulged three-way-junction structure with catalytically important nucleotides residing in the bulge. A highly conserved GA mismatch is also crucial for...
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PMID: 19142882
PDF is available here.
PDF is available here.
Abstract
These results offer a potential of using allele-specific 10-23 DNAzyme in combination with other cancer therapies to achieve better effectiveness on cancer treatment....
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PMID: 19014906
PDF is available here.
PDF is available here.
Abstract
Cancer is a disease state in which the cells in our body undergo mutations at the genetic level and are transformed, acquiring the ability to replicate limitlessly. Conventional cancer treatment involves the use of surgery and cytotoxic chemotherapy and/or radiotherapy, which have the potential of h...
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PMID: 19126291
PDF is available here.
PDF is available here.
Abstract
Two states, two applications! An Ag(+)-mediated DNAzyme switch has been designed to detect Ag(+) and cysteine with high sensitivity and selectivity. In the closed state, Ag(+) turns on the switch through the formation of cytosine-Ag(+)-cytosine base pairs, whereas adding cysteine turns off the open...
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PMID: 19222076
PDF is available here.
PDF is available here.
Abstract
Base pairs, magic hands: An additional base-pairing duplex is utilized to control the folding topologies of a bimolecular G-quadruplex formed by two G-rich single-stranded DNAs (see picture), which is dependent on the position of base pairs. This study clearly reveals an important intrinsic role of...
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PMID: 19137559
PDF is available here.
PDF is available here.
Abstract
Two G-quadruplex DNAs are able to form peroxidase-like DNAzymes selectively promoted by K(+), which provides a label-free approach to colorimetric detection of K(+) with the highest sensitivity ever reported.
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PMID: 19283297
PDF is available here.
PDF is available here.
Abstract
Many important cellular processes are regulated by small endogenous noncoding RNAs known as microRNAs (miRNAs). The precise molecular function of many miRNAs is unknown; different loss-of-function methods are required to gain insight into the biology of these small RNA molecules. Nucleic acid enzyme...
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PMID: 19229913
PDF is available here.
PDF is available here.
Abstract
A method for the amplified analysis of a gene sequence by a Fok I/DNA machine is described. A tailored nucleic acid that includes a recognition sequence for the analyzed gene and a hybridized sequence of the gene itself acting as the "fuel" substrate of the "machine". Upon recognition of the gene by...
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PMID: 19049271
PDF is available here.
PDF is available here.
Abstract
We describe experiments to reveal design aspects of the selection strategy that favor site-selective and regioselective synthesis of native 3'-5' RNA linkages. The results also reveal that an explicit selection pressure for RNA substrate sequence generality must be developed if the deoxyribozymes ar...
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PMID: 19005599
PDF is available here.
PDF is available here.
Abstract
With a designed high-activity DNAzyme as the catalytic label, an ultrasensitive chemiluminescence thrombin aptasensor is developed, enabling a 10- to 100-fold improvement in the detection sensitivity as compared with previous counterparts.
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PMID: 18997938
PDF is available here.
PDF is available here.
Abstract
Colorimetric uranium sensors based on uranyl (UO2(2+)) specific DNAzyme and gold nanoparticles (AuNP) have been developed and demonstrated using both labeled and label-free methods. In the labeled method, a uranyl-specific DNAzyme was attached to AuNP, forming purple aggregates. The presence of uran...
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PMID: 18837498
PDF is available here.
PDF is available here.
Journal of Molecular Biology (381)4 2008
Abstract
We report a systematic replacement of a number of bases within the magnesium-folded DNAzyme-substrate complex with thio- and halogen-substituted base analogues, which were then photochemically activated to generate contact cross-links within the complex. Mapping of the cross-links revealed a strikin...
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PMID: 18586041
PDF is available here.
PDF is available here.
Abstract
In order to develop the oligonucleotides to abolish an expression of TEL-AML1 chimeric RNA, which is a genetic aberration that causes the acute lymphoblastic leukemia (ALL), hammerhead ribozymes and deoxyoligoribozymes that can specifically cleave TEL-AML1 fusion RNA were designed. Constructs of the...
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PMID: 18627769
PDF is available here.
PDF is available here.
Abstract
DNA aptamers carrying Pt nanoparticles were prepared by the reaction of DNA aptamers (without functionalization with biotin, thiol, or other reactive groups) with K 2[PtCl 4] in solution at 60-90 degrees C. The DNA-Pt complexes possessed peroxidase enzymatic activity while retaining the specific bin...
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PMID: 18665606
PDF is available here.
PDF is available here.
Abstract
DNAzymes are oligonucleotides capable of specific catalysis of target mRNA. To date, a delivery vehicle for DNAzymes has not been developed. Chitosan is a biomaterial obtained abundantly in nature. A biocompatible c-jun (an oncogene) DNAzyme nanoparticle formulated from chitosan was found to be more...
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PMID: 18465314
PDF is available here.
PDF is available here.
Abstract
An electrochemical DNAzyme sensor for sensitive and selective detection of lead ion (Pb(2+)) has been developed, taking advantage of catalytic reactions of a DNAzyme upon its binding to Pb(2+) and the use of DNA-Au bio-bar codes to achieve signal enhancement. A specific DNAzyme for Pb(2+) is immobil...
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PMID: 18627134
PDF is available here.
PDF is available here.
Abstract
The discovery of naturally occurring catalytic RNA (RNA enzymes, or ribozymes) in the 1980s immediately revised the view of RNA as a passive messenger that solely carries information from DNA to proteins. Because DNA and RNA differ only by the absence or presence of a 2'-hydroxyl group on each ribos...
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PMID: 18654692
PDF is available here.
PDF is available here.
Abstract
We tested whether 2'-O-methyl-modified Dz, designed to recognize a highly-conserved RNA sequence located within the core-E1 coding region, could recognize and cleave its target sequence in the structural context of a functional HCV RNA molecule. Dz858-4-OMe contains four 2'-O-methyl nucleotide deriv...
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PMID: 18514249
PDF is available here.
PDF is available here.
Cancer Gene Therapy (15)8 2008
Abstract
We therefore hypothesized that Aurora kinase A might work as a valuable target for prostate cancer treatment. To test this hypothesis, we used DNAzyme technology to inhibit Aurora kinase A expression and evaluated the effects of DNAzymes as therapeutic agents to treat prostate cancer. In an in vitro...
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PMID: 18404163
PDF is available here.
PDF is available here.
Abstract
Although much work has concentrated on using CDzs for regulating gene expression, their potential as nucleic acid medicines has diminished substantially, supplanted by simple antisense oligonucleotides and, more recently, by small interfering RNAs (siRNAs). It seems unlikely that CDzs will have clin...
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PMID: 18613760
PDF is available here.
PDF is available here.
Abstract
The oncogene c-Jun has been found to be up-regulated in a variety of cancers including osteosarcoma. DNA enzymes (DNAzymes) are oligonucleotides capable of specific catalysis of target mRNA. A c-Jun DNAzyme inhibited the growth and metastasis of osteosarcoma in an orthotopic spontaneously metastasiz...
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PMID: 18708361
PDF is available here.
PDF is available here.
Cancer Letters (265)2 2008
Abstract
We have designed several LMP1-specific DNAzymes and tested their effect on cell proliferation and apoptosis in LMP1-positive cells. Here, we show that active DNAzymes down-regulated the expression of the EBV oncoprotein LMP1 and inhibited cellular signal transduction pathways abnormally activated by...
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PMID: 18353539
PDF is available here.
PDF is available here.
Journal of General Virology (89)Pt 7 2008
Abstract
We have designed several Dzs, targeting different regions of HCV IRES specific for 1b and also sequences conserved across genotypes. The RNA cleavage and translation inhibitory activities of these molecules were tested in a cell-free system and in cell culture using transient transfections. The majo...
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PMID: 18559927
PDF is available here.
PDF is available here.
Virus Research (135)1 2008
Abstract
These results suggested that pk1 regulates polyhedrin promoter driven transcription of AcNPV, and the effect of one gene expression on that of other can be studied by DNAzyme knockdown....
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PMID: 18353480
PDF is available here.
PDF is available here.
Abstract
We report that DNA hybridization can be utilized for sequence-specific reversible self-assembly of well-ordered 2D colloidal arrays. Two essential indicators of DNA-hybridization mediated assembly were confirmed: thermal reversibility and sequence specificity. The arrays melted at 50 degrees C and r...
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PMID: 18540602
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (133)7 2008
Abstract
The amplified detection of DNA or of single-base mismatches in DNA is achieved by the use of nucleic acid-functionalized magnetic particles that separate the recognition duplexes and, upon amplification, yield chemiluminescence-generating DNAzymes as reporter units. The analysis of M13 phage ssDNA i...
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PMID: 18575646
PDF is available here.
PDF is available here.
Abstract
Deoxyribozymes (DNA enzymes; DNAzymes) are catalytic DNA sequences. Using the technique of in vitro selection, individual deoxyribozymes have been identified that catalyze RNA cleavage, RNA ligation, and a growing range of other chemical reactions. DNA enzymes have been used in vitro for application...
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PMID: 18373062
PDF is available here.
PDF is available here.
Abstract
The oncogene c-Jun has been found to be up-regulated in a variety of cancers, including osteosarcoma. Doxorubicin is a frontline chemotherapeutic against osteosarcoma, but is limited by toxicity. DNAzymes are oligonucleotides capable of specific catalysis of target mRNA. A biocompatible c-Jun DNAzym...
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PMID: 18645001
PDF is available here.
PDF is available here.
Abstract
We have thus sought to firstly provide background into the construct and mechanism of action of DNAzymes, with a discussion of recent improvements in design. Secondly, we have examined the effects of DNAzyme-mediated gene inhibition in in vitro studies of both endothelial and smooth muscle migration...
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PMID: 18454170
PDF is available here.
PDF is available here.
Abstract
Recent studies have demonstrated the potential of DNAzymes for therapy of various diseases via mRNA target-specific cleavage. One such target, the basic region-leucine zipper protein c-Jun, has been targeted and efficacy seen in such pathologies as cancer, ocular neovascularisation, arterial thicken...
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PMID: 18604982
PDF is available here.
PDF is available here.
Chemical Society Reviews (37)6 2008
Abstract
Catalytic nucleic acids (DNAzymes or ribozymes) are selected by the systematic evolution of ligands by exponential enrichment process (SELEX). The catalytic functions of DNAzymes or ribozymes allow their use as amplifying labels for the development of optical or electronic sensors. The use of cataly...
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PMID: 18497928
PDF is available here.
PDF is available here.
Abstract
These results suggest that LL-37 may modulate the expression of VEGF and c-IAP-2 via HIF-1alpha in human keratinocytes....
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PMID: 18412861
PDF is available here.
PDF is available here.
Lab On a Chip (8)5 2008
Abstract
Due to the numerous toxicological effects of lead, its presence in the environment needs to be effectively monitored. Incorporating a biosensing element within a microfluidic platform enables rapid and reliable determinations of lead at trace levels. A microchip-based lead sensor is described here t...
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PMID: 18432350
PDF is available here.
PDF is available here.
Abstract
DNAzyme cascades activated by Pb(2+)- or L-histidine-dependent DNAzymes yield the horseradish peroxidase-mimicking catalytic nucleic acids that enable the colorimetric or chemiluminescence detection of Pb(2+) or L-histidine.
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PMID: 18354802
PDF is available here.
PDF is available here.
Abstract
Intranasal administration of gd21 prevented airway inflammation and mucus production and inhibited development of airway hyperresponsiveness to methacholine in models of acute allergic airway inflammation. Similar effects were also detected in a model of chronic experimental asthma. Interestingly, g...
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PMID: 18325571
PDF is available here.
PDF is available here.
Abstract
These results suggest that cellular targeting using colloidal gold appears feasible for DNAzyme delivery....
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PMID: 18444511
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)5 2008
Abstract
We systematically mutated the catalytic core of 8-17 and measured the cleavage activity of each mutant deoxyribozyme against all 16 possible chimeric (RNA/DNA) dinucleotide junctions. We observed sequence-function relationships that suggest how the following non-conserved positions in the catalytic...
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PMID: 18203744
PDF is available here.
PDF is available here.
Journal of Molecular Biology (375)4 2008
Abstract
We report a deoxyribozyme with a unique structure that contains a two-tiered guanine quadruplex interlinked to a Watson-Crick duplex. Through in vitro selection, sequence mutation, and methylation interference, we show the presence of both the two-tiered guanine-quadruplex and two helical regions co...
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PMID: 18054790
PDF is available here.
PDF is available here.
ChemBioChem (9)2 2008
Abstract
A bifunctional aptamer that includes two aptamer units for cocaine and adenosine 5'-monophosphate (AMP) is blocked by a nucleic acid to form a hybrid structure with two duplex regions. The blocked bifunctional aptamer assembly is used as a functional structure for the simultaneous sensing of cocaine...
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PMID: 18161727
PDF is available here.
PDF is available here.
Abstract
Abstract
These results indicate that Pb(II), the most active metal ion, competes for Tb(III) binding differently from other metal ions such as Zn(II), suggesting that Pb(II) may bind to a different site from that for the other metal ions including Zn(II) and Tb(III)....
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PMID: 18688837
PDF is available here.
PDF is available here.