Abstract
We found that the binding of only one gold nanoparticle to a plasmid-cisplatin complex containing 3197 base pairs increases by a factor of 3 the efficiency of the chemotherapeutic agent cisplatin to produce double-strand breaks in irradiated DNA. Furthermore, adding two cisplatin molecules and one g...
|
PMID: 19580513
PDF is available here.
Abstract
We designed. The entire mtDNA sequence was 16 773 base pairs (bp) in length, and the base composition was: A-5,357 bp (31.9%); C-4,444 bp (26.5%); G-2,428 bp (14.5%); T-4,544 bp (27.1%). The structural characteristics [0] of the P. uncia mitochondrial genome were highly similar to these of Felis cat...
|
PMID: 18431688
PDF is available here.
Abstract
We report identification of a nuclear-encoded bacterial histone-like protein (PfHU) involved in DNA compaction in the apicoplast. PfHU is associated with apicoplast DNA and is expressed throughout the parasite's intra-erythocytic cycle. The protein binds DNA in a sequence nonspecific manner with a m...
|
PMID: 18663012
PDF is available here.
Abstract
We used site-specific crosslinking to create chemical connections at the three interfaces between the three constituent polypeptides of the ring, thereby creating covalently closed cohesin rings. As predicted by the ring entrapment model, this procedure produced dimeric DNA-cohesin structures that a...
|
PMID: 18596691
PDF is available here.
Abstract
The 46-kb plasmid pSK41 is the prototype of a family of staphylococcal conjugative multiresistance plasmids. Sequence analyses have revealed the presence of a putative resolvase gene, res, on pSK41, and identical or related genes carried by other staphylococcal multiresistance plasmids. Carriage of...
|
PMID: 18492061
PDF is available here.
Abstract
We surveyed influenza sequences from a broad taxonomic distribution and collected 65 full-length genomes representing avian, human and "classic" swine H1N1 lineages in addition to numerous other swine (H1N2, H3N1, and H3N2), human (H2N2, H3N2, and H5N1), and avian (H1N1, H4N6, H5N1, H6N1, H6N6, H6N8...
|
PMID: 18353690
PDF is available here.
Abstract
We provide the first phylogenetic analysis of notothenioids using both mtDNA and nuclear gene sequences for a comprehensive sampling of all major lineages in the clade. Maximum parsimony and Bayesian analyses of aligned mtDNA genes, an aligned nuclear gene (S7 ribosomal protein intron 1), and combin...
|
PMID: 18249562
PDF is available here.
Abstract
Most of the recognized species of the genus Dionda inhabit drainages of the Gulf of Mexico from central Mexico to central Texas, USA, and have been considered a monophyletic group based on morphological, osteological, and allozyme investigations. Phylogenetic relationships of 15 species of Dionda an...
|
PMID: 18353691
PDF is available here.
Abstract
We demonstrated that neither the ND10 components PML and hDaxx nor NFkappaB activation represent the target for MG132....
|
PMID: 18242185
PDF is available here.
Abstract
We compared eight plasmids from five Arthrobacter strains in order to identify putative core maintenance genes for replication, segregation, and conjugation. Iteron like sequences were identified on some of the plasmids; however, no genes with obvious similarity to known replication sequences such a...
|
PMID: 18241920
PDF is available here.
Abstract
We program diverse molecular self-assembly and disassembly pathways using a 'reaction graph' abstraction to specify complementarity relationships between modular domains in a versatile DNA hairpin motif. Molecular programs are executed for a variety of dynamic functions: catalytic formation of branc...
|
PMID: 18202654
PDF is available here.
Abstract
We tackled some of them using a new combination of maximum likelihood-based methods, developed in order to practice as safe and careful concatenations as possible. First, we used the application concaterpillar on carefully aligned core genes. This application uses a hierarchical likelihood-ratio tes...
|
PMID: 17940208
PDF is available here.
Abstract
Relationships of the genera in the Enterobacteriaceae containing plant pathogenic species: Brenneria, Dickeya, Enterobacter, Erwinia, Pantoea, Pectobacterium, and Samsonia, were investigated by comparison of their nucleotide and peptide sequences of atpD, carA, recA, and the concatenated sequences....
|
PMID: 17451899
PDF is available here.
Abstract
We show here that DNA ligase IV, which performs the joining step of NHEJ, is degraded as a consequence of adenovirus infection. Degradation is dependent upon E4 34k and E1b 55k, functional proteasomes, and the activity of cellular cullin 5, a component of the adenoviral ubiquitin ligase. DNA ligase...
|
PMID: 17459921
PDF is available here.
Abstract
The soluble factors produced either by Ehrlich's ascites carcinoma (EAC) or thymic adherent cells (TAC) of tumor-bearing mice comprising of CD11b(+) and CD11c(+) antigen-presenting cells caused a sharp decrease in prolactin (PRL)-induced ConA-mediated effect on survival of PNA(+) thymocytes. Similar...
|
PMID: 17442407
PDF is available here.
Abstract
We analyze a Bayesian model for estimating species trees that accounts for the stochastic variation expected for gene trees from multiple unlinked loci sampled from a single species history after a coalescent process. Application of the model to a 106-gene data set from yeast shows that the set of g...
|
PMID: 17392434
PDF is available here.
Abstract
We found that Mhr1 is required for the hypersuppressiveness of HS [ori5] [rho(-)] mtDNA harboring ori5, one of the replication origins of normal ([rho(+)]) mtDNA. In addition, we detected an Ntg1-stimulated double-strand break at the ori5 locus. Purified Ntg1, a base excision repair enzyme, introduc...
|
PMID: 17116696
PDF is available here.
Abstract
Here, we present SCaFoS, a tool that quickly assembles phylogenomic datasets containing maximal phylogenetic information while adjusting the amount of missing data in the selection of species, sequences and genes. Starting from individual sequence alignments, and using monophyletic groups defined by...
|
PMID: 17288575
PDF is available here.
Abstract
We have established that supermatrices derived from data sets composed mainly by genes suspected to be essential for bacterial cellular life perform better on the recovery of vertical signal than those composed by widely distributed genes. In addition, we show that the "Transcription" category of ge...
|
PMID: 17288580
PDF is available here.
Aron M AM Geurts,
Lara S LS Collier,
Jennifer L JL Geurts,
Leann L LL Oseth,
Matthew L ML Bell,
David D Mu,
Robert R Lucito,
Susan A SA Godbout,
Laura E LE Green,
Scott W SW Lowe,
Betsy A BA Hirsch,
Leslie A LA Leinwand and
David A DA Largaespada
Abstract
We used the SB system to mutate a region of mouse Chromosome 11 in a forward-genetic screen for recessive lethal and viable phenotypes. This work represents the first reported use of an insertional mutagen in a phenotype-driven approach. The phenotype-driven approach was successful in both recoverin...
|
PMID: 17009875
PDF is available here.
Abstract
The model bryophyte Physcomitrella patens exhibits high frequencies of gene targeting when transformed with DNA constructs containing sequences homologous with genomic loci. 'Targeted gene replacement' (TGR) resulting from homologous recombination (HR) between each end of a targeting construct and t...
|
PMID: 17090599
PDF is available here.
Abstract
For the number of replicates needed for accurate estimates of support in resampling plans, a diagram is provided that helps to address the question whether apparently different support values really differ significantly. It is shown that the use of random addition cycles and parsimony ratchet iterat...
|
PMID: 16255783
PDF is available here.
Abstract
Cloning and sequencing artifacts contaminating SAGE libraries could be eliminated using simple pre-screening procedure to increase the reliability of the data....
|
PMID: 15491492
PDF is available here.
Abstract
Members of the pentameric ligand-gated ion channel family, comprising GABA(A) receptors, nicotinic acetylcholine receptors, glycine receptors and 5-HT3 receptors, are involved in information transfer at both synapses and the neuromuscular junction. However, receptors that are composed of five subuni...
|
PMID: 15559253
PDF is available here.
Abstract
We tested the negative model by using a deletion mutant devoid of all prohead genes and FI in an in vivo cos cleavage assay; in this deleted phage, the cohesive ends were not cut. When lambda proheads and gpFI were provided in vivo via a second prophage, cutting was restored, and gpFI was required,...
|
PMID: 15066036
PDF is available here.
Abstract
I without accessory sites, were selected in E. coli. Some single mutants can resolve a res x site I plasmid (that is, with one res and one site I), but two or more activating mutations are necessary for efficient resolution of a site I x site I plasmid. Site I x site I resolution by hyperactive muta...
|
PMID: 14763971
PDF is available here.
Abstract
Stretching of DNA molecules helps to resolve detail during the fluorescence microscopy of both single DNA molecules and single DNA-protein complexes. To make stretching occur, intricate procedures of specimen preparation and manipulation have been developed in previous studies. By contrast, the pres...
|
PMID: 14731300
PDF is available here.
Abstract
We used pulsed-field gel electrophoresis, restriction fragment mapping, and fluorescence microscopy of individual DNA molecules to analyze the structure of chloroplast DNA (cpDNA) from shoots of ten to 14 day old maize seedlings. We find that most of the cpDNA is in linear and complex branched forms...
|
PMID: 14698291
PDF is available here.
Abstract
Our previous study suggested that tandem multimers ("concatemers") formed mainly by the Mhr1p (a yeast nuclear gene-encoded mtDNA-recombination protein)-dependent pathway are required for mtDNA partitioning into buds with concomitant monomerization. The transmission of a few randomly selected clones...
|
PMID: 14565971
PDF is available here.
Abstract
We have developed a method for genomic representation using Type IIB restriction endonucleases. Representation by concatenation of restriction digests, or RECORD, is an approach to sample the fragments generated by cleavage with these enzymes. Here, we show that the RECORD libraries may be used for...
|
PMID: 15329383
PDF is available here.
Abstract
We describe here that DEK has a clear preference for supercoiled and four-way junction DNA. In the presence of topoisomerase II, DEK stimulates intermolecular catenation of circular DNA molecules. DEK also increases the probability of intermolecular ligation of linear DNA molecules by DNA ligase. Th...
|
PMID: 14627833
PDF is available here.
Abstract
Successive viral passage of Spodoptera exigua multicapsid nucleopolyhedrovirus (SeMNPV) in the S. exigua cell line Se301 leads to the rapid accumulation of the non-hr origin of DNA replication (ori) as large concatemers. Passage of SeMNPV in two other S. exigua cell lines, SeUCR1 and SeIZD2109, did...
|
PMID: 14726243
PDF is available here.