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DNA, Helminth (0):
Recent article
DNA, Helminth
Avian Diseases (55)1 2011
Abstract
An outbreak of ascaridiasis occurred in 10-wk-old guinea fowl (Numida meleagris) on a commercial farm. Birds had exhibited elevated mortality (11.66%) in the previous week, as well as increased water consumption, weakness, anorexia, and stunted growth. Numerous nematodes, occasionally occluding the i...
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PMID: 21500654
PDF is available here.
PDF is available here.
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Systematic Parasitology (77)3 2010
Abstract
We also report what we interpret here as intraspecific variation in the ITS2 rDNA between individuals of E. manilensis from Lutjanus vitta recovered off the Great Barrier Reef and New Caledonia....
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PMID: 20960090
PDF is available here.
PDF is available here.
Parasitology (137)13 2010
Abstract
We describe a detailed analysis of the molecular epidemiology and phylogeography of S. mansoni from Lake Victoria. In total, 388 cytochrome oxidase 1 (COI) sequences were obtained from 25 sites along the Ugandan, Tanzanian and Kenyan shorelines of Lake Victoria, and 122 unique barcodes were identifi...
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PMID: 20561396
PDF is available here.
PDF is available here.
Abstract
Diagnosis of Strongyloides stercoralis is often delayed owing to patients presenting with nonspecific gastrointestinal complaints, a low parasite load and irregular larval output. Although several diagnostic methods exist to detect the presence of S. stercoralis there is no gold standard. In immunoc...
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PMID: 20733481
PDF is available here.
PDF is available here.
Abstract
In order to reveal DNA types of aspermic Fasciola forms in Japan, Fasciola specimens obtained from eight prefectures that had not been previously reported were analyzed for DNA of ribosomal internal transcribed spacer 1 (ITS1) and mitochondrial NADH dehydrogenase 1 (ND1) gene. Five combinations in D...
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PMID: 20467211
PDF is available here.
PDF is available here.
Parasitology Research (107)5 2010
Abstract
The MYND-type zinc finger protein (MYND-ZF) is a large group of proteins containing the MYND domain which play an important role in protein-protein interactions. A cDNA clone encoding a novel MYND-ZF was isolated and identified from a Clonorchis sinensis (C. sinensis) adult cDNA library. The open re...
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PMID: 20680338
PDF is available here.
PDF is available here.
Abstract
Phylogenetic relationships of 6 species in the trematode subfamily Haplorchiinae were analyzed using small and large subunit of ribosomal DNA genes (18S rDNA and 28S rDNA) and internal transcribed spacer subunit II (ITS2) region as molecular markers. Maximum Likelihood and Bayesian inference analyse...
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PMID: 20601104
PDF is available here.
PDF is available here.
Veterinary Parasitology (172)1-2 2010
Abstract
The study was designed to compare the in vitro egg hatch test (EHT) and molecular allele-specific polymerase chain reaction PCR (AS-PCR) methods as tools for detection of benzimidazole resistance in Haemonchus contortus, a nematode parasite of small ruminants. Comparisons were made during the course...
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PMID: 20684865
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)9 2010
Abstract
The length of the telomeric DNA in nine species of planarians inhabiting Lake Baikal and one Siberian species from Baikal rivers was determined using Southern hybridization. According to preliminary estimations, it varied in the range of 25-30 kb (Rimacephalus arecepta, Rimacephalus pulvinar, Soroce...
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PMID: 21061625
PDF is available here.
PDF is available here.
Journal of Parasitology (96)4 2010
Abstract
Gyrocerviceanseris passamaquoddyensis n. gen., n. sp. is described from the gills of silver hake, Merluccius bilinearis , in Passamaquoddy Bay, southwestern New Brunswick, Canada. Diagnostic features include an oval haptor with asymmetrically distributed marginal hooks (3 pairs anterior, 5 pairs pos...
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PMID: 20738197
PDF is available here.
PDF is available here.
Journal of Parasitology (96)4 2010
Abstract
We used a PCR assay to obtain novel genome sequences from cercariae isolates of 3 European bird schistosome species ( Trichobilharzia franki , Trichobilharzia szidati , and Trichobilharzia regenti ) collected from freshwater ponds in Belorussia and Russia. We applied RAPD-fingerprinting using 1 rand...
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PMID: 20677938
PDF is available here.
PDF is available here.
Abstract
We identified a novel GST gene of Taenia solium metacestodes (TsMs), which is a causative agent of neurocysticercosis. The 804 bp-long cDNA encoded a 639 bp open reading frame (212 amino acid polypeptide), which exhibited the structural motif and domain organisation characteristic of GST. It formed...
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PMID: 20385139
PDF is available here.
PDF is available here.
Abstract
These results indicate a major role for SmPlk1 in parasite reproduction and suggest its importance as a potential new target against schistosomiasis.
Copyright 2010 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved....
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PMID: 20350550
PDF is available here.
PDF is available here.
Abstract
CA88 is the first long nuclear repetitive DNA sequence identified in the blood fluke, Schistosoma mansoni. The assembled S. mansoni sequence, which contains the CA88 repeat, has 8,887 nucleotides and at least three repeat units of approximately 360 bp. In addition, CA88 also possesses an internal CA...
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PMID: 20721481
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
Thioredoxin reductase (TrxR) is an evolutionary diverse enzyme. In higher eukaryotes, TrxR contains a selenocysteine in the active site which confers high activity and a broad substrate range. The parasitic nematode Haemonchus contortus contains two TrxRs, a cytoplasmic enzyme HcTrxR1 with a selenoc...
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PMID: 20495929
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
Natural infection with Trichinella has been described in more than 150 mammalian species. However, few reports of Trichinella infection in wild animals have come from Argentina. In this study, muscle tissue was obtained from wild animals in Argentina with the aim of evaluating the presence of Trichi...
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PMID: 20424859
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
Diphyllobothrium latum is a widespread intestinal parasite, which has a great clinical relevance, but there are no sequences of its nuclear genome. In this paper, a repetitive element in the D. latum genome is firstly described. The adult D. latum was obtained in the result of expulsion from intesti...
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PMID: 20440623
PDF is available here.
PDF is available here.
Abstract
We present a survey of R. reniformis ESTs that were sequenced from a sedentary parasitic female cDNA library. Cluster analysis of 2004 high quality ESTs produced 123 contigs and 508 singletons for a total of 631 R. reniformis unigenes. BLASTX analyses revealed that 39% of all unigenes showed similar...
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PMID: 20346373
PDF is available here.
PDF is available here.
Abstract
Cysticerci of Taenia sp. from two elks (Alces alces) in Finland were characterized using morphological criteria and sequences of two mitochondrial DNA regions. The host species, size, structure and location of the cysticerci indicated that they might belong to Taenia krabbei, a circumpolar species o...
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PMID: 20304093
PDF is available here.
PDF is available here.
Abstract
An anomalous tapeworm with abnormal segmentation was obtained from a 6-year-old boy in Japan. The tapeworm consisted of proglottids with slanted anterior and posterior margins of proglottids and 4-6 sets of reproductive organs arranged between the margins. The morphology of the tapeworm did not corr...
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PMID: 20035897
PDF is available here.
PDF is available here.
Abstract
We collected 6 populations of O. viverrini from different places in Cambodia, Lao PDR, and Thailand, along both sides of the Mekong River, and analyzed the population structure of these using the mitochondrial nad1 gene as a marker. The results of the DNA polymorphism measurements, by theta-w (theta...
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PMID: 20197110
PDF is available here.
PDF is available here.
Abstract
Rhabdias casiraghii n. sp. and R. kibiraensis n. sp. are described from Trioceros ellioti from Burundi and co-infection was observed in one of the host specimens. Distinctive characters between these species are, among others, the mouth and buccal capsule in front view, both of which are round in th...
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PMID: 20597435
PDF is available here.
PDF is available here.
Journal of Parasitology (96)3 2010
Abstract
We extracted all the nematode fauna present on 114 individuals of G. stercorosus. By direct sequencing using the 18S SSU, we provide a barcode for all nematodes isolated from the beetle samples. In total, 5,002 dauer-stage nematodes were sequenced, which included Pristionchus spp., Koerneria spp. (D...
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PMID: 20557197
PDF is available here.
PDF is available here.
An ortholog of the ecdysone receptor protein (EcR) from the parasitic nematode Haemonchus contortus.
Abstract
We report the cloning of cDNA encoding an EcR ortholog (HcEcR) from H. contortus mRNA expressed during L3. Phylogenetically, this and the putative EcR from Brugia malayi form a separate branch between arthropod EcRs and liver X receptors. Two isoforms of HcEcR differ in the inclusion/omission of a 3...
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PMID: 20226216
PDF is available here.
PDF is available here.
Abstract
We have identified a D. immitis nuclear receptor, DiEcR that shows strong sequence similarity to the insect EcR and shares many of its biochemical properties, including ligand-dependent activation of transcription. However, unlike most insect EcRs, DiEcR requires a ligand-activated RXR partner to ex...
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PMID: 20170689
PDF is available here.
PDF is available here.
Abstract
We still do not know how these alleles are contributing to resistance in vivo. To understand the phenomenon of anthelmintic resistance in detail we need to link changes in the genes of individual parasites with their ability to resist the effects of anthelmintic exposure. We have determined the geno...
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PMID: 20211658
PDF is available here.
PDF is available here.
Abstract
Our results show that fish intermediate hosts play a role in the transmission of metastrongyloid nematodes of harbour porpoises and harbour seals.
(c) 2010 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved....
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PMID: 20123100
PDF is available here.
PDF is available here.
Abstract
Parastrongylus cantonensis is a parasite of murid rodents that can infect humans and cause health problems as eosinophilic meningitis. Although it is endemic in south Asia, the Pacific islands, Australia, USA, and a few Caribbean islands, it has been extended to new geographical regions. In the Cana...
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PMID: 20153283
PDF is available here.
PDF is available here.
Avian Pathology (39)2 2010
Abstract
Experiments were first conducted to compare and evaluate different methods of Ascaridia galli larval recovery from the chicken intestine. The number of larvae recovered from the intestinal wall of chickens infected with 1000 embryonated A. galli eggs and killed 15 days post infection (p.i.) by three...
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PMID: 20390541
PDF is available here.
PDF is available here.
Parasitology (137)4 2010
Abstract
We describe a genetic mapping approach for the discovery of DNA markers for anthelmintic resistance in Haemonchus contortus. We crossed a multi-drug resistant field isolate of H. contortus with a well-characterized laboratory strain susceptible to 4 drug classes. The F2 were separately selected with...
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PMID: 19835651
PDF is available here.
PDF is available here.
Abstract
We show that the antirecombinase RTEL-1 is required to prevent excess meiotic COs, probably by promoting meiotic synthesis-dependent strand annealing. Two distinct classes of meiotic COs are increased in rtel-1 mutants, and COI and homeostasis are compromised. We propose that RTEL-1 implements the s...
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PMID: 20203049
PDF is available here.
PDF is available here.
Abstract
Species delineation in parthenogenetic tropical species of Meloidogyne nematodes is particularly difficult although they are strictly apomictic. In fact, parthenogenesis in Meloidogyne nematodes is a recent phenomenon and the structure of the genetic diversity is mainly explained by crosses prior to...
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PMID: 19900581
PDF is available here.
PDF is available here.
Abstract
In this study, Ascaris DNA was extracted and sequenced from a medieval archaeological sample in Korea. While Ascaris eggs were confirmed to be of human origin by archaeological evidence, it was not possible to pinpoint the exact species due to close genetic relationships among them. Despite this sho...
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PMID: 20428686
PDF is available here.
PDF is available here.
Abstract
Journal of Helminthology (84)1 2010
Abstract
The aim of this study was to test the hypothesis of the presence of hybrids between Schistosoma guineensis and S. haematobium in the Estuaire province (Western Gabon). Egg morphometry and single-stranded conformational polymorphism (SSCP) analysis on adult worms were used in order to characterize th...
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PMID: 19622197
PDF is available here.
PDF is available here.
Abstract
A 59-year-old Korean man complained of a painless scrotal hard nodule and weak urine stream. The ultrasound scan revealed a 2.2-cm sized round heteroechogenic nodule located in the extratesticular area. Microscopic hematuria was detected in routine laboratory examinations. On scrotal exploration, mu...
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PMID: 20333286
PDF is available here.
PDF is available here.
Journal of Helminthology (84)1 2010
Abstract
Schistosoma haematobium and S. bovis are widespread schistosome species causing human and cattle schistosomiasis, respectively, in Africa. The sympatric occurrence of these two species and their ability to infect the same Bulinus intermediate snail hosts necessitates precise methods of identificatio...
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PMID: 19646307
PDF is available here.
PDF is available here.
Journal of Helminthology (84)1 2010
Abstract
Trichinella zimbabwensis has been detected in wild and farmed Nile crocodiles (Crocodylus niloticus) and in wild monitor lizards (Varanus niloticus) of several African countries, but it has never been detected in mammals in nature, in spite of its infectivity to rodents, pigs, foxes and monkeys unde...
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PMID: 19580688
PDF is available here.
PDF is available here.
Experimental Parasitology (124)3 2010
Abstract
We developed polymerase chain reaction (PCR) primers specific for human schistosome species. The PCR products were obtained in a species-specific manner (479 bp, Schistosoma mansoni; 365 bp, S. haematobium; 614 bp, S. japonicum; 303 bp, S. mekongi) and were detectable from 0.01 pg of total worm DNA...
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PMID: 19948171
PDF is available here.
PDF is available here.
Parasitology (137)2 2010
Abstract
We applied a combined molecular and morphological approach to resolve the taxonomic status of Saccocoelium spp. parasitizing sympatric mullets (Mugilidae) in the Mediterranean. Eight morphotypes of Saccocoelium were distinguished by means of multivariate statistical analyses: 2 of Saccocoelium obesu...
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PMID: 19849887
PDF is available here.
PDF is available here.
Abstract
Among the helminths infecting ruminants in China are three taxa belonging to the genus Fasciola: F. hepatica, F. gigantica and the so-called 'intermediate form' that appears to lie between these two species. Based on the sequences of the second internal-transcribed spacers (ITS-2) within the parasit...
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PMID: 20149293
PDF is available here.
PDF is available here.
Abstract
Square wave electroporation facilitates introduction of transgenes into the schistosome egg. Electroporation was more effective for the transduction of eggs with pseudotyped MLV than simply soaking the eggs in virions. These findings underscore the potential of targeting the schistosome egg for germ...
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PMID: 20126309
PDF is available here.
PDF is available here.
Abstract
We found that resveratrol may provide protection against hazardous radiation. Pre-treatment with resveratrol extended both the maximum and mean life span of irradiated C. elegans. Resveratrol acted as a strong radical scavenger and regulated superoxide dismutase (SOD) expression. In addition, resver...
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PMID: 20679743
PDF is available here.
PDF is available here.
Abstract
These results showed that copro-DNA diagnosis was useful for the differential diagnosis of O. viverrini and H. taichui infection, which could not be discriminated by microscopy....
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PMID: 20578479
PDF is available here.
PDF is available here.
Abstract
Opisthorchiasis is one of the significant naturofocal diseases in Russia. The diagnosis of opisthorchiasis may mask some diseases, such as opisthorchiasis, metorchiasis, and clonorchiasis - biohelminthoses that are induced by various representatives of the family Opisthorchiidae-Opisthorchis felineu...
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PMID: 20614520
PDF is available here.
PDF is available here.
Abstract
According updates on molecular genetics, the development of human schistosomes in Asia with subsequent migration to the African continent is considered to be the most probable course of events. Generally, there are 2 hypotheses of the genus Schistoma. A hypothesis of Gondvana origin was based on sna...
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PMID: 20608188
PDF is available here.
PDF is available here.
Journal of Helminthology (83)4 2009
Abstract
We hypothesize that the newly found traits may contribute to the maintenance of this mutualistic association of the bacterium with its nematode host....
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PMID: 19216823
PDF is available here.
PDF is available here.
Journal of Helminthology (83)4 2009
Abstract
Diphtherostomum brusinae was first recorded by the present study in the north of Portugal. Sporocysts, containing cercariae and encysted metacercariae, were observed in the gonads and digestive gland of the gastropod Nassarius reticulatus. Metacercariae were also found infecting the foot, mantle bor...
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PMID: 19265558
PDF is available here.
PDF is available here.
Journal of Helminthology (83)4 2009
Abstract
Trichinella larvae isolated from the thigh muscle of a wild boar, Sus scrofa, captured from Gilan Province, northern Iran, was processed for DNA analysis. Polymerase chain reaction (PCR) for amplification of the 5S rDNA fragment demonstrated a 700 bp band on agarose gel. Analysis of DNA sequencing b...
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PMID: 19531274
PDF is available here.
PDF is available here.
Journal of Helminthology (83)4 2009
Abstract
We report the in vitro effect of insulin on the larval stages of T. crassiceps (ORF strain) and T. solium. In vitro exposure of T. crassiceps cysticerci to insulin was found to stimulate this parasite's reproduction twofold with respect to control values, while the same treatment had no effect on T....
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PMID: 19549345
PDF is available here.
PDF is available here.
Abstract
We observed 62 specimens collected from Korean residents and analyzed them by morphological features and nucleotide sequences of mitochondrial cox1 gene as well as the ITS1 region. Adult tapeworms were examined after carmine or trichrome stain. Longitudinal sections of the gravid proglottids showed...
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PMID: 19967084
PDF is available here.
PDF is available here.
Abstract
Human clonorchiasis, caused by infection with the trematode Clonorchis sinensis, is a common health problem in East Asia. In an attempt to develop a new, sensitive method for the diagnosis of the disease, the use of a real-time PCR (targeting the internal-transcribed-spacer-2 sequence of the parasit...
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PMID: 19695156
PDF is available here.
PDF is available here.
Abstract
I repeat, pWb12 repeat, pWb-35 repeat, and LDR repeat for Wuchereria bancrofti and Hha I repeat, glutathione peroxidase gene, mitochondrial DNA for Brugia malayi), and universal primers, multiplex-PCR, PCR-restriction fragment length polymorphism (PCR-RFLP), PCR-enzyme linked immunosorbent assay (PC...
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PMID: 19842372
PDF is available here.
PDF is available here.
Abstract
Haplorchis taichui specific primers were designed using a high annealing temperature random amplified polymorphic DNA (HAT-RAPD) PCR method and 18 arbitrary primers (Operon Technologies) to generate polymorphic DNA profiles for 13 different parasites. The H. taichui specific fragment was screened. A...
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PMID: 19842374
PDF is available here.
PDF is available here.
Abstract
Dirofilaria immitis is an important filarial parasite of dogs and cats, and a useful model for human filariasis. Current diagnostic tests for heartworm infection in animals rely on the presence of fecund female worms (usually found 6.5 months post-infection or later) and therefore fail to detect pre...
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PMID: 8728997
PDF is available here.
PDF is available here.
Abstract
I restriction fragments using a subclone of Tc15 as a probe was carried out. Restriction map and sequence data were compiled for 3 clones containing a 145 bp highly repetitive sequence (designated TcREP) which shared homology with the original pTc15 clone. TcREP hybridized to a tandemly repeating se...
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PMID: 7970890
PDF is available here.
PDF is available here.
Abstract
I collagen over different pH and temperature conditions, but it did not cleave immunoglobulin. While most cathepsin cysteine proteinases are unable to digest collagen, mammalian cathepsin K, adult F. hepatica FhCL2 and the plant zingipain can also cleave collagen and substrates with Pro in P2 positi...
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PMID: 19383516
PDF is available here.
PDF is available here.
Abstract
In this study, 4847 expressed sequenced tags (ESTs) from mixed stages of the migratory plant-parasitic nematode Ditylenchus africanus (peanut pod nematode) were investigated. It is the first molecular survey of a nematode which belongs to the family of the Anguinidae (order Rhabditid...
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PMID: 19383517
PDF is available here.
PDF is available here.
Abstract
We describe type 1 cystatin, a cysteine protease inhibitor, as a major released antigen of the tropical liver fluke Fasciola gigantica (FgStefin-1). Immunohistochemical analysis showed that FgStefin-1 is abundant in (a) tissue of tegumental type, including oral and ventral sucker, pharynx, genital a...
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PMID: 19416741
PDF is available here.
PDF is available here.
FASEB Journal (23)8 2009
Abstract
We have cloned and characterized. The cDNA encodes a predicted 304-aa protein (SmAQP) that is found largely in the parasite tegument by immunolocalization and is most highly expressed in the intravascular life stages. Treatment of parasites with short interfering RNAs targeting the SmAQP gene result...
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PMID: 19364765
PDF is available here.
PDF is available here.