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DNA, Plant (1):
Recent article
DNA, Plant
American Naturalist (178)S1 2011
Abstract
We evaluate alternative range limit models using a multilocus data set based on DNA sequences and microsatellites along with field demographic data from the annual plant Clarkia xantiana ssp. xantiana. Our data suggest that central and peripheral populations have very large historical and current ef...
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PMID: 21956091
PDF is available here.
PDF is available here.
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Abstract
DNA-based techniques have proved to be very useful methods to study trophic relationships between pests and their natural enemies. However, most predators are best defined as omnivores, and the identification of plant-specific DNA should also allow the identification of the plant spe...
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PMID: 21092379
PDF is available here.
PDF is available here.
Abstract
Although pecans are associated with IgE-mediated food allergies, the allergens responsible remain to be identified and characterized. The 2S albumin gene was amplified from the pecan cDNA library. Dot-blots were used to screen the recombinant protein with pecan allergic patients' serum. The affinity...
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PMID: 21395309
PDF is available here.
PDF is available here.
Abstract
We used the specific spatial expression patterns of SbtM1 promoter β-d-glucuronidase (GUS) fusions to isolate cis elements that confer AM responsiveness. A promoter deletion and substitution analysis defined two cis regions (region I and II) in the SbtM1 promoter necessary for AM-induced GUS activi...
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PMID: 21261463
PDF is available here.
PDF is available here.
BMC Plant Biology (11) 2011
Abstract
We have identified and annotated the SERK genes in this species. Using degenerate PCR and database mining, eight more SERK-like genes have been identified and these have been shown to be expressed. The amplification and sequencing of several different PCR products from one of these genes is consiste...
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PMID: 21385462
PDF is available here.
PDF is available here.
Abstract
I (PSI) is a multisubunit pigment-protein complex that uses light energy to transfer electrons from plastocyanin to ferredoxin. Application of genetic engineering to photosynthetic reaction center proteins has led to a significant advancement in our understanding of primary electron transfer events...
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PMID: 20960138
PDF is available here.
PDF is available here.
Abstract
We suggest that ITS sequences would be more suitable to serve as markers for authentication of Herba Leonuri than matK does....
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PMID: 21476215
PDF is available here.
PDF is available here.
Abstract
Paramutation refers to the process by which homologous DNA sequences communicate in trans to establish meiotically heritable expression states. Although mechanisms are unknown, current data are consistent with the hypothesis that the establishment and heritable transmission of specific chromatin sta...
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PMID: 21030647
PDF is available here.
PDF is available here.
Abstract
We report a transporter, Nrat1 (Nramp aluminum transporter 1), specific for trivalent Al ion in rice. Nrat1 belongs to the Nramp (natural resistance-associated macrophage protein) family, but shares a low similarity with other Nramp members. When expressed in yeast, Nrat1 transports trivalent Al ion...
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PMID: 20937890
PDF is available here.
PDF is available here.
Abstract
We describe the positional cloning of two soybean genes Rj2 and Rfg1 that restrict nodulation with specific strains of Bradyrhizobium japonicum and Sinorhizobium fredii, respectively. We show that Rj2 and Rfg1 are allelic genes encoding a member of the Toll-interleukin receptor/nucleotide-binding si...
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PMID: 20937853
PDF is available here.
PDF is available here.
Abstract
We show that rice endosperm DNA is hypomethylated in all sequence contexts. Non-CG methylation is reduced evenly across the genome, whereas CG hypomethylation is localized. CHH methylation of small transposable elements is increased in embryos, suggesting that endosperm demethylation enhances transp...
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PMID: 20937895
PDF is available here.
PDF is available here.
Two-photon fluorescence imaging of DNA in living plant turbid tissue with carbazole dicationic salt.
Abstract
Three carbazole dicationic salts, namely 3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (BMVC), 9-ethyl-3,6-bis(1-methyl-4-vinylpyridinium) carbazole diiodide (9E-BMVC) and 9-ethyl-3,6-bis(1-hydroxyethyl-4-vinylpyridinium)carbazole diiodide (9E-BHVC), were synthesized successfully. Their pho...
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PMID: 20714660
PDF is available here.
PDF is available here.
Biotechnology Letters (32)10 2010
Abstract
A 1681 bp PsPR10 promoter was isolated from Pinus strobus and a series of 5'-deletions were fused to the β-glucuronidase (GUS) reporter gene and introduced into tobacco. GUS activity in P796 (-796 to +69) construct transgenic plant roots was similar with that of P1681 and higher than those of the P...
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PMID: 20495947
PDF is available here.
PDF is available here.
Abstract
Carotene desaturation, an essential step in the carotenoid biosynthesis pathway, is catalyzed by two carotene desaturases, phytoene desaturase (PDS) and ζ-carotene desaturase (ζ-carotene desaturase, ZDS). Full-length cDNAs designated TaPDS and TaZDS were cloned from wheat (Triticum aestivum cv. Ch...
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PMID: 20013057
PDF is available here.
PDF is available here.
New Phytologist (188)2 2010
Abstract
Our study reveals that interspecific gene flow affects the genome evolution in Arabidopsis.
© The Authors (2010). Journal compilation © New Phytologist Trust (2010)....
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PMID: 20673288
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)10 2010
Abstract
We studied genomes of 22 cultivated flax varieties and 24 wild species from six sections of the genus Linum L. The chromosome numbers were exactly determined in the karyotypes of all studied species, and all individual chromosomes were identified by the C/DAPI-banding pattern and localization of 26S...
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PMID: 21254551
PDF is available here.
PDF is available here.
Fitoterapia (81)6 2010
Abstract
Tribulus terrestris L. (Zygophyllaceae) is one of the highly traded raw drugs and also used as a stimulative food additive in Europe and USA. While, Ayurvedic Pharmacopoeia of India recognizes T. terrestris as Goksura, Tribulus lanuginosus and T. subramanyamii are also traded by the same name raisin...
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PMID: 20083169
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)9 2010
Abstract
Using methods of molecular cytogenetics and molecular genetics, components of highly repetitive DNA fraction and specifically transposable elements (TEs), were examined in diploid ancestors of cultivated wheat, the species of the Sitopsis group (Aegilops Poaceae). It was demonstrated that TEs were h...
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PMID: 21061631
PDF is available here.
PDF is available here.
Journal of Plant Physiology (167)12 2010
Abstract
We isolated the full-length cDNA of AhIRT1 (iron-regulated transporter 1) from peanut and characterized how iron deficiency and intercropping affected its iron-transporting ability. Functional complementation with AhIRT1 restored normal growth of the yeast mutant fet3fet4 (defective in both high- an...
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PMID: 20430476
PDF is available here.
PDF is available here.
Journal of Plant Physiology (167)12 2010
Abstract
These results suggest that MaArf is induced by ethylene in regulating postharvest banana ripening. Finally, subcellular localization assays showed the MaArf protein in the cytoplasm.
Copyright 2010 Elsevier GmbH. All rights reserved....
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PMID: 20435371
PDF is available here.
PDF is available here.
Abstract
A collection of 4117 fertile T-DNA lines has been generated by Agrobacterium-mediated transformation of the diploid community standard line Bd21 of Brachypodium distachyon. The regions flanking the T-DNA left and right borders of the first 741 transformed plants were isolated by adap...
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PMID: 20374523
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)8 2010
Abstract
The plant family Orchidaceae has a great economic value (ornamental and medical uses, beside the aromatic features). Traditionally, identification of orchid species has relied heavily on morphological features. These features, however, are either not variable enough between species or too plastic to...
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PMID: 20873203
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)8 2010
Abstract
Inter-simple sequence repeat (ISSR) amplification was used to analyze polymorphisms of microsatellite sequences in the honeysuckle genome and to evaluate genetic diversity among fourteen Polish and Russian blue honeysuckle accessions (Lonicera caerulea var. edulis, L. caerulea no. 7661, L. caerulea...
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PMID: 20873204
PDF is available here.
PDF is available here.
Genetika (Moskva) (46)8 2010
Abstract
We estimated the genetic diversity within and among populations of wild Helianthus from five districts of Mozambique using RAPD markers. The 44 accessions studied grouped into four major clusters exhibiting structured variability with regard to geographic origin. A high level of genetic diversity (H...
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PMID: 20873205
PDF is available here.
PDF is available here.
Abstract
We produced 9463 bp of sequences from eight non-coding chloroplast regions for 146 species in 26 genera and 5 outgroups. The loci sequenced were atpI/H, psaA-ORF170, rpl32-trnL, rpoB-trnC, rps16-trnQ, trnD/T, trnS/G, and trnT/L. Phylogenetic analyses using maximum parsimony and Bayesian inference su...
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PMID: 20381627
PDF is available here.
PDF is available here.
Abstract
The evolution of the eastern Asian and eastern North American disjunction of the witch-hazel genus Hamamelis L. (Hamamelidaceae) was examined through phylogenetic and biogeographic analyses. Phylogenetic relationships of all Hamamelis species were reconstructed using parsimony and Bayesian analyses...
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PMID: 20171295
PDF is available here.
PDF is available here.
Abstract
We present a statistically highly supported phylogeny of Genlisea based on three chloroplast loci. The most recent common ancestor of Genlisea most likely was of Neotropical origin and characterized by pedicels that are recurved in fruit, a strongly glandular inflorescence, and bivalvate capsule deh...
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PMID: 20226867
PDF is available here.
PDF is available here.
Abstract
We analyzed the single copy nuclear gene coding for elongation factor G (EF-G) from 28 accessions of polyploid Elymus species and 45 accessions of diploid Triticeae species in order to investigate origin of the Y genome and its relationship to other genomes in the tribe Triticeae. Sequence compariso...
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PMID: 20363342
PDF is available here.
PDF is available here.
Abstract
We estimated higher-level relationships within Orchidaceae, focusing on subfamilies and tribes. DNA sequences of part of the low-copy nuclear protein gene Xdh were obtained for 154 taxa including 126 genera of Orchidaceae and outgroup families of Asparagales. The general topology of the Xdh trees is...
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PMID: 20211743
PDF is available here.
PDF is available here.
Abstract
We isolated four distinct avocado cell proliferation-related genes and investigated their expression characteristics, comparing normal fruit (NF) and SF developmental patterns. Three cDNAs termed PaCYCA1, PaCYCB1 and PaPCNA, encoding two mitotic cyclins and a proliferating cell nuclear antigen (PCNA...
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PMID: 20544218
PDF is available here.
PDF is available here.
Abstract
Duckweeds (Lemnaceae) are extremely reduced in morphology, which made their taxonomy a challenge for a long time. The amplified fragment length polymorphism (AFLP) marker technique was applied to solve this problem. 84 clones of the genus Lemna were investigated representing all 13 accepted Lemna sp...
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PMID: 20526614
PDF is available here.
PDF is available here.
Abstract
We also report a novel transcription module, designated 'chloroplast biogenesis module' (CBM) in the FKBP16-1 promoter of cereals that also appears to be involved in the regulation of additional genes involved in chloroplast biogenesis or other aspects of plant development. The results point to cons...
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PMID: 20570161
PDF is available here.
PDF is available here.
Abstract
We now report the shared presence of two rps3 introns, namely the rps3i74 and the rps3i249, in the mitochondria of all the classes representing the surviving lineages of gymnosperms, and unveil several lineages experiencing intron loss. Interestingly, the rps3 intron gains and losses within the four...
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PMID: 20605476
PDF is available here.
PDF is available here.
Abstract
A wide range of methods are commonly used for preserving environmental samples prior to molecular analyses. However, the effect of these preservation methods on fungal DNA is not understood. The objective of this study was to test the effect of eight different preservation methods on the quality and...
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PMID: 20470836
PDF is available here.
PDF is available here.
Abstract
We used the germination percentage, root length, weight gain and micronucleus (MN) frequency as indicators of cytotoxicity. Additionally to the cytological analysis, DNA analyses were performed in root tips meristems of Vicia faba seeds treated with refinery wastewater. Heavy metal concentrations in...
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PMID: 21186721
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
Interspecific hybrids between the related wild barley species Hordeum marinum and H. bulbosum were generated and tested regarding their chromosomal stability and chromatin properties. Unlike in H. vulgare x H. bulbosum hybrid embryos, there was no effect of temperature on uniparental chromosome elim...
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PMID: 20551604
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
We focused on establishing more dissection lines for 1RS and on obtaining PCR-based markers specific to 1RS. We established 66 1RS dissection lines carrying 1RS segments of chromosome 1R derived from a common wheat cultivar 'Burgas 2' and obtained 27 markers. We conducted a PCR analysis using the di...
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PMID: 20551617
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
We now can detect smaller chromosomal and DNA rearrangements, diminutions and amplifications of repeats and changes of the epigenetic status of introgressed regions.
Copyright 2010 S. Karger AG, Basel....
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PMID: 20628252
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
Maize is a highly diverse species on the gene sequence level. With the recent development of methods to distinguish each of the 10 pairs of homologues in somatic root tip spreads, a wide collection of maize lines was subjected to karyotype analysis to serve as a reference for the community and to ex...
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PMID: 20551613
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
We selected 21 cucumber fosmids anchored by SSR markers as chromosome-specific cytological markers for melon chromosomes. Moreover, with the help of melon centromeric satellite DNA repeats CentM, 45S rDNA and 5S rDNA, sequential FISH with 3 sets of multi-fosmid cocktails were conducted on the same m...
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PMID: 20551614
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
This review summarizes older as well as recent data about the model dioecious plant Silene latifolia. This plant has been the subject of more than one hundred years of research efforts and its most conspicuous property is huge and well differentiated heteromorphic sex chromosomes, XX in females and...
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PMID: 20551610
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
We have examined chloroplast DNA insertions in maize chromosomes using fluorescence in situ hybridization (FISH). Probes to detect the nuclear-plastid sequences (NUPTs) were generated using 14 overlapping fragments of the chloroplast genome. Using current FISH methods, detection of NUPTs on mitotic...
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PMID: 20628248
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
We did not observe any evidence of pSp1B16 and pGc1R1 sequence elimination, which is in contrast to previous studies using similar hybrids and repeats. The presented data suggest that the genomic changes in synthetic amphiploids observed in previous studies might be caused by homoeologous recombinat...
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PMID: 20551615
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
We are developing a robust and reliable molecular cytogenetic assay for recombination in this species, which will be used to gauge the success of our forward and reverse genetic interventions. Single-locus bacterial artificial chromosome clones and rDNA probes identify the 7 somatic chromosomes of t...
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PMID: 20551612
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
The large bread wheat genome (1C approximately 17 Gbp) contains a preponderance of repetitive DNA and the species is polyploid. These characteristics together serve to hamper the molecular analysis of the wheat genome. Its complexity can, however, be reduced by using flow cytometry to isolate indivi...
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PMID: 20501977
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
Plants have a wide range of genome sizes. The length of each DNA molecule is usually much longer than the diameter of the cell and the length of each metaphase chromosome is effectively shortened to progress through mitosis. Thus some questions arise, such as: How is genomic DNA folded and shortened...
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PMID: 20628249
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
We have devised a method to produce panels for the simultaneous mapping of all chromosomes of the D subgenome of hexaploid wheat. In this approach, seeds of hexaploid wheat (AABBDD) are irradiated and the surviving plants are crossed to tetraploid wheat (AABB) to produce a mapping panel based on qua...
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PMID: 20501975
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
We reevaluated 5 F(1) hybrids using high-resolution, electron microscopic examination of pachytene chromosome (= synaptonemal complex) spreads to determine whether any minor structural changes had occurred among species in the tomato clade, which were not easily visible using light microscopic analy...
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PMID: 20551606
PDF is available here.
PDF is available here.
Cytogenetic and Genome Research (129)1-3 2010
Abstract
The 35S ribosomal DNA (rDNA) intergenic spacer (IGS) of Allium cernuum is examined. Initial sequencing of IGS clones revealed that some rDNA units contain a truncated retrotransposon sequence most similar to members of the Copia superfamily. Fluorescence in situ hybridisation (FISH) to metaphase chr...
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PMID: 20516662
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
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PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
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PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
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PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
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PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
|
PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
|
PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
|
PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
|
PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
|
PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
|
PMID: 20675570
PDF is available here.
PDF is available here.
Plant Cell Online (22)7 2010
Abstract
We show here that FLP and MYB88 are nuclear proteins with DNA binding preferences distinct from other known MYBs. To identify possible FLP/MYB88 transcriptional targets, we used chromatin immunoprecitation (ChIP) followed by hybridization to Arabidopsis whole genome tiling arrays. These ChIP-chip da...
|
PMID: 20675570
PDF is available here.
PDF is available here.