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DNA, Protozoan (1):
Recent article
DNA, Protozoan
Parasitology (137)13 2010
Abstract
We aimed to define a suitable method for cryopreserving viable Dientamoeba trophozoites. A modified BD medium, TYGM-9, Loeffler's slope medium, Robinson's medium, Medium 199, Trichosel and a Tritrichomonas fetus medium were compared, using cell counts, for their ability to support the growth of D. f...
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PMID: 20609278
PDF is available here.
PDF is available here.
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Parasitology (137)13 2010
Abstract
We used 12 microsatellite markers developed for Leishmania braziliensis to genotype 28 strains of the main species of the Leishmania guyanensis complex (i.e. L. guyanensis and L. panamensis) collected in Ecuador and Peru. The important heterozygote deficits observed in these populations are similar...
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PMID: 20609264
PDF is available here.
PDF is available here.
Parasitology (137)13 2010
Abstract
We used flow cytometry analysis to separate expelled L. caulleryi gametocytes from infected chicken blood and to analyse cell populations and sorting by FACS efficiency. Infected blood cells stained with SYTO-24 showed a specific area on 2-dimensional scattergrams compared to uninfected blood. The s...
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PMID: 20619066
PDF is available here.
PDF is available here.
Parasitology (137)13 2010
Abstract
Myxosporean parasites are significant parasites of fishes not only for their apparent high diversity but also for their potential impact on fish health and/or marketability. Regardless, our knowledge of most myxosporeans, especially those found in elasmobranch hosts, is superficial. A study of multi...
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PMID: 20619061
PDF is available here.
PDF is available here.
Abstract
We worked with highly characterized T. cruzi and T. rangeli isolates from different biologic origins and geographic areas of Colombia, and they were analyzed by RFLP and PCR amplification of variable region of minicircles and Southern blot analysis. Our results and experimental conditions demonstrat...
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PMID: 20850248
PDF is available here.
PDF is available here.
Abstract
ISME Journal (4)9 2010
Abstract
We present a broad-ranging microscopic, molecular and microspectrophotometric analysis of uncultured P. sphagnophila collected from four field locations in Nova Scotia, Canada. Consistent with its morphological characteristics, 18S ribosomal DNA (rDNA) phylogenies indicate that P. sphagnophila is sp...
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PMID: 20376101
PDF is available here.
PDF is available here.
Parasitology (137)10 2010
Abstract
This study investigated the ability of the Pacific oyster, Crassostrea gigas, to act as a carrier or reservoir of the protistan Bonamia ostreae. Studies were carried out independently in Ireland and in Spain. Naïve C. gigas were exposed to B. ostreae both in the field and in the laboratory via natu...
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PMID: 20388237
PDF is available here.
PDF is available here.
Parasitology (137)10 2010
Abstract
Yellow-eyed penguins on Stewart Island were identified with a Leucocytozoon spp. of a novel lineage in association with a high regional incidence of chick mortality (n=32, 100% mortality) during the November 2006 to January 2007 breeding season. Fourteen chicks from Stewart Island were examined post...
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PMID: 20557665
PDF is available here.
PDF is available here.
Abstract
We report the results of pathological, microbiological and molecular investigations used to characterize unidentified skin lesions in palmate newts (Lissotriton helveticus) from Larzac, France. We confirm that the lesions are the result of infection with a novel dermocystidium-like parasite, which i...
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PMID: 20450984
PDF is available here.
PDF is available here.
Veterinary Parasitology (172)1-2 2010
Abstract
These results suggested that C-Cp12-Cp21-DNA may provide an effective means of eliciting humoral and cellular responses and generating protective immunity against C. parvum infections in BALB/c mice.
(c) 2010 Elsevier B.V. All rights reserved....
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PMID: 20541869
PDF is available here.
PDF is available here.
Veterinary Parasitology (172)1-2 2010
Abstract
In the present study a highly species-specific oligonucleotide sequence of Tritrichomonas foetus 18S rRNA was used to design an antisense probe for identification of T. foetus in formalin-fixed and paraffin-embedded histological specimens by means of fluorescence in situ hybridization (FISH). Using...
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PMID: 20447769
PDF is available here.
PDF is available here.
Abstract
We have successfully isolated a pool of DNA aptamers, named SELH3, which binds to Leishmania infantum H3 with high affinity and specificity. Thus, it appears that this novel anti-H3 aptamer population may be of potential application as a diagnostic system for leishmaniosis....
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PMID: 20545478
PDF is available here.
PDF is available here.
Abstract
We can conclude that the LAMP assay provides us an accurate and highly sensitive test method to detect C. parvum oocyst and cyst of G. lamblia, in place of labor-intensive and experience-dependent microscopic examination, in clinical laboratories....
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PMID: 20860168
PDF is available here.
PDF is available here.
Parasitology Research (107)3 2010
Abstract
Spores of the myxozoan parasite Myxobolus turpisrotundus Zhang 2009 were observed for the first time bearing caudal appendages. Most spores had the typical Myxobolus spp. morphology, but approximately 10% of spores possessed a spore body that was slightly elongated with a short tail projecting from...
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PMID: 20512504
PDF is available here.
PDF is available here.
Abstract
We determined the prevalence of G. duodenalis in two species of seal (Halichoerus grypus, Phoca vitulina vitulina and Phoca vitulina richardsi) from the east and west coasts of the USA, sequenced two loci from G. duodenalis-positive samples to assess molecular diversity and examined G. duodenalis di...
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PMID: 20361967
PDF is available here.
PDF is available here.
Eukaryotic Cell (9)7 2010
Abstract
We identified the ciliate-specific Defective in IES Excision 5 (DIE5) genes of Paramecium tetraurelia (PtDIE5) and Tetrahymena thermophila (TtDIE5) as orthologs that encode nuclear proteins expressed exclusively during development. Abrogation of PtDie5 protein (PtDie5p) function by RNA interference...
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PMID: 20495055
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
Parasitology Research (107)2 2010
Abstract
We report the first case in Colombia of chagasic megacolon with cardiomyopathy associated with the T. cruzi I lineage. This finding suggests that in Colombia, as well as in other northern countries of South America and throughout Central America, where T. cruzi I is endemic, cardiomyopathy may not b...
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PMID: 20502919
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
We have investigated the expression level of surface cruzipain in 16 field isolates belonging to the genotype TCI (n = 8) and TCII (n = 8) of T. cruzi. By means of flow cytometry analyses, using an anti-cruzipain polyclonal antibody, we observed a highly heterogeneous pattern of surface cruzipain mo...
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PMID: 20512588
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
Due to the scarcity of information related to the epidemiology of Cryptosporidium infection in passerine birds, this study aimed to determine the periodicity of fecal shedding of Cryptosporidium spp. oocysts, after natural infection, and its clinical signs, mortality, and molecular characterization....
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PMID: 20407911
PDF is available here.
PDF is available here.
Parasitology Research (107)2 2010
Abstract
We conducted cross-sectional studies in the New York City Watershed to ensure a valid estimate of the risk associated with Cryptosporidium infection in dairy herds. Our aims were to obtain species-specific estimates of the prevalence of Cryptosporidium in dairy cattle and to investigate seasonal var...
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PMID: 20397026
PDF is available here.
PDF is available here.
Critical taxonomic revision of Parabasalids with description of one new genus and three new species.
Abstract
We propose a new classification of Parabasalia which is congruent with both ultrastructural and molecular-phylogenetic studies. We identify six main parabasalid lineages and give them the rank of class: Hypotrichomonadea, Trichomonadea, Tritrichomonadea, Cristamonadea, Trichonymphea, and Spirotricho...
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PMID: 20093080
PDF is available here.
PDF is available here.
Pelagodinium gen. nov. and P. béii comb. nov., a dinoflagellate symbiont of planktonic foraminifera.
Abstract
The taxonomic status of the free-living stage of the dinoflagellate Gymnodinium béii, symbiont of the foraminifer Orbulina universa, was reassessed on the basis of detailed morpho-genetic analyses. Electron microscopy observations revealed previously undescribed morphological features of the cell t...
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PMID: 20149979
PDF is available here.
PDF is available here.
An evaluation of the use of the LSU rRNA D1-D5 domain for DNA-based taxonomy of eukaryotic protists.
Abstract
We studied the application of the large subunit (LSU) rRNA gene fragments (D1-D5) regarding their usefulness to discriminate between a wide range of heterotrophic nanoflagellates. Phylogenetic analyses based on the LSU rRNA fragments showed similar results compared to phylogenetic trees based on the...
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PMID: 20153693
PDF is available here.
PDF is available here.
Abstract
An investigation into the diversity of the dinoflagellate Alexandrium was carried out during August 2007 within two fjordic sea lochs in the Shetland Isles, Scotland. The co-occurrence in the water column of the non-toxic West European (W.E. or Gr.III) and the neurotoxic North American (N.A. or Gr.I...
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PMID: 20093079
PDF is available here.
PDF is available here.
Abstract
We have determined SSU rRNA gene sequences for Kofoidiniaceae, those of the type Spatulodinium (=Gymnodinium) pseudonoctiluca and another Spatulodinium species, as well as of two species of Kofoidinium, and the first gene sequence of Leptodiscaceae, that of Abedinium (=Leptophyllus) dasypus. These t...
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PMID: 20188628
PDF is available here.
PDF is available here.
Trends in Parasitology (26)7 2010
Abstract
We should first acknowledge that these novel trypanosomes exist and then take steps to investigate their host range, pathogenicity to livestock and response to chemotherapy.
Copyright 2010 Elsevier Ltd. All rights reserved....
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PMID: 20382076
PDF is available here.
PDF is available here.
Biology Letters (6)3 2010
Abstract
I discuss eukaryotic deep phylogeny and reclassify the basal eukaryotic kingdom Protozoa and derived kingdom Chromista in the light of multigene trees. I transfer the formerly protozoan Heliozoa and infrakingdoms Alveolata and Rhizaria into Chromista, which is sister to kingdom Plantae and arguably...
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PMID: 20031978
PDF is available here.
PDF is available here.
Abstract
The Leishmune vaccine has been used in endemic areas to prevent canine visceral leishmaniasis in Brazil, but cytokine production induced by vaccination has rarely been investigated in dogs. This study aimed to evaluate the immune response of dogs vaccinated with Leishmune FML vaccine (Fort Dodge) ag...
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PMID: 20132994
PDF is available here.
PDF is available here.
Abstract
Abstract
We provide the first report of infections in treeshrews, describe the morphology of encysted parasites using light and transmission electron microscopy, and place this agent within a phylogenetic context by sequencing and comparing its 18S ribosomal DNA to that of related parasites. Muscle infection...
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PMID: 20026242
PDF is available here.
PDF is available here.
Abstract
The myxozoan Zschokkella hildae Auerbach, 1910, was detected with a prevalence of 100% in cultured Atlantic cod, Gadus morhua L. aged 1+ from a culture facility on the west coast of Scotland. Sporogonic stages of Z. hildae, plasmodia producing 2-5 mature spores, were located predominantly in the col...
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PMID: 20026425
PDF is available here.
PDF is available here.
Abstract
We have reported that Plasmodium falciparum genome contains a number of DEAD box helicases. In the present study we report the cloning, expression and characterization of one of the novel members of DEAD box family from P. falciparum. Our results indicate that it is a homologue of Has1p from yeast a...
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PMID: 20153446
PDF is available here.
PDF is available here.
Abstract
Toxoplasma gondii-derived heat shock protein 70 (T.g.HSP70) was proven to induce lethal anaphylactic reaction in T. gondii-infected mice through platelet-activating factor (PAF)-mediated, but not classical IgE-dependent, pathway via TLR4/MyD88 signal pathway. The effector cells generating PAF and ca...
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PMID: 20346412
PDF is available here.
PDF is available here.
Abstract
As part of investigations into the cause of a waterborne outbreak of Cryptosporidium hominis infection linked to a mains water supply, surface waters and wastewater treatment plants were tested for Cryptosporidium spp. Oocyst counts in base flow surface water samples ranged from nil to 29 per 10 l....
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PMID: 20154394
PDF is available here.
PDF is available here.
Abstract
Apart from a single record in a shark, there have been no published studies conducted on Giardia genotypes in fish. The present study investigated the prevalence of Giardia in cultured fingerlings (n=227), wild freshwater (n=227) and wild marine/estuarine species (n=255) of fish in Western Australia...
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PMID: 20026124
PDF is available here.
PDF is available here.
Abstract
We suggest that organisms clustering with the sheep and cattle isolates analysed in the present study be named E. bovis.
(c) 2010 Australian Society for Parasitology Inc. All rights reserved....
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PMID: 20359482
PDF is available here.
PDF is available here.
Experimental Parasitology (125)2 2010
Abstract
Little is known about the epidemiology of Cryptosporidium in Jordan and no genotyping studies have been conducted on Cryptosporidium isolates from humans or animals from Jordan. Genotyping of 44 Cryptosporidium isolates from Jordanian children at the 18S rRNA locus and a unique diagnostic locus iden...
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PMID: 20109456
PDF is available here.
PDF is available here.
Direct detection and genotyping of Toxoplasma gondii in meat samples using magnetic capture and PCR.
Abstract
We developed a method for detection and quantification of T. gondii. The method involved preparation of crude DNA extract from hundred gram samples of meat, magnetic capture of T. gondii DNA and, quantitative real-time PCR targeting the T. gondii 529-bp repeat element. The detection limit of this as...
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PMID: 20350771
PDF is available here.
PDF is available here.
Abstract
We provide a molecular-based identification of both the amoeba host and the two bacterial endosymbionts. Analysis of the 18S rRNA gene revealed that this strain is the sister-group to Glaeseria, for which we proposed the name Saccamoeba lacustris. The rod-shaped endosymbiont was identified as a memb...
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PMID: 20347279
PDF is available here.
PDF is available here.
Abstract
Noctiluca scintillans (Macartney) Kofoid et Swezy, 1921 is an unarmoured heterotrophic dinoflagellate with a global distribution, and has been considered as one of the ancestral taxa among dinoflagellates. Recently, 18S rDNA, actin, alpha-, beta-tubulin, and Hsp90-based phylogenies have shown the ba...
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PMID: 20347580
PDF is available here.
PDF is available here.
Abstract
A strain of naked amoeba isolated from pikeperch (Sander lucioperca (L.)) kidney tissue has been characterized using light- and transmission electron microscopy. Sequencing of SSU rDNA and phylogenetic analysis based on a broad dataset of sequences completed our study. All data obtained suggest that...
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PMID: 20347581
PDF is available here.
PDF is available here.
Abstract
The objective of the present paper is to develop and apply a multiplex polymerase chain reaction (mPCR) based reverse line blot (RLB) hybridization assay to facilitate the diagnosis of genital infections by detection of seven recognized or putative genital pathogens (Neisseria gonorrhoeae, Chlamydia...
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PMID: 20498099
PDF is available here.
PDF is available here.
Abstract
The living morphology, infraciliature and silverline system of two small marine Euplotes species, E. orientalis spec. nov. and E. raikoviAgamaliev, 1966, isolated from a sandy beach near Qingdao, China, were investigated. Euplotes orientalis is characterized by a combination of features including th...
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PMID: 20172700
PDF is available here.
PDF is available here.
Parasitology (137)4 2010
Abstract
Salivarian trypanosomes pose a substantial threat to livestock, but their full diversity is not known. To survey trypanosomes carried by tsetse in Tanzania, DNA samples from infected proboscides of Glossina pallidipes and G. swynnertoni were identified using fluorescent fragment length barcoding (FF...
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PMID: 19961657
PDF is available here.
PDF is available here.
Abstract
We cloned and produced T. carassii calreticulin (rTcaCRT), and generated a rabbit polyclonal antibody to the recombinant protein. The incubation of parasites with rabbit anti-rTcaCRT affinity-purified IgG antibody indicated substantial CRT levels on the surface of trypanosomes, as well as internal s...
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PMID: 19913050
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (28)2 2010
Abstract
Pf-LDH gene was same to reference sequences(DQ198262), while there is a single nucleotide difference at the position 666 between Pv-LDH gene and reference sequences (DQ060151). The coding region of the two genes contained 951 bp encoding a 316-amino-acid residue. Compared with Pf-LDH, Pv-LDH showed...
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PMID: 20666310
PDF is available here.
PDF is available here.
Abstract
Although Trypanosoma (Megatrypanum) theileri, a blood parasite of bovid species, is spread widely throughout the world, it has never been reported in Taiwan. When an anti-coagulated blood sample from febrile dairy cattle was directly smeared, no parasite was observed. However, a highly distinctive m...
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PMID: 20009352
PDF is available here.
PDF is available here.
Epidemiology and Infection (138)4 2010
Abstract
We conducted a cohort study to identify risk factors for giardiasis, and stool and environmental samples were analysed. Consuming tap water was significantly associated with illness (risk ratio 4.7, 95% confidence interval 1.5-14.4). Drinking-water samples were coliform-contaminated and a suspect Gi...
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PMID: 19751538
PDF is available here.
PDF is available here.
Abstract
We obtained a cDNA library of ciliates Euplotes, the titer of which was about 2.437 x 10(7) cfu/mL, which could suffice for functional gene screen. By using the class II polypeptide release factor (eRF3) as bait protein, we obtained some putative genes, including a partial cDNA putatively encoding R...
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PMID: 20499635
PDF is available here.
PDF is available here.
Abstract
Histopathologic examination revealed it as nodular vasculitis. Leishmania smear showed suspicious parasites. Although leishmania culture was negative, PCR was positive for Leishmania. The patient was considered to have CL and was treated with systemic meglumine antimoniate for 14 d. Three days after...
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PMID: 20465667
PDF is available here.
PDF is available here.
Abstract
The presence of genetically different strains of malarial parasites in cases of human malaria is a severe drawback in the successful control of the disease. In Sri Lanka, although this species accounts for 60%-80% of all of the cases of clinical malaria that occur each year, the genetic complexity o...
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PMID: 20406577
PDF is available here.
PDF is available here.
Abstract
The performance of the less expensive SYBR-Green-based PCR assay, for quantifying Leishmania chagasi in smears of bone-marrow aspirates from naturally infected, mongrel dogs, was recently compared with that of a similar PCR based on TaqMan chemistry. Aspirates were obtained from 36 infected dogs and...
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PMID: 20406580
PDF is available here.
PDF is available here.
Abstract
In an effort to improve the diagnosis of intestinal amoebiasis, a real-time PCR has been used for the detection and differentiation of Entamoeba histolytica and E. dispar infections in African or South American immigrants who live in Spain. Faecal samples from all of the 130 subjects had apparently...
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PMID: 20406581
PDF is available here.
PDF is available here.
Abstract
We have established a long term program aiming at pinpointing the sources of surface water, drinking water, and environmental contamination, working with the water-supply industry. Total 43 sources of drinking water samples were selected, and a total of 167 samples were analyzed using the Method 162...
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PMID: 20333284
PDF is available here.
PDF is available here.
Abstract
Pentatrichomonas hominis is considered a commensal protozoan in the large intestine of a number of mammalian hosts, such as cats, dogs, and non-human primates. The resulting infections, which can induce diarrhea, have been attributed to opportunistic overgrowth of P. hominis. This study was performe...
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PMID: 20333280
PDF is available here.
PDF is available here.
Abstract
We evaluated this approach in 42 patients presenting with ocular or psychotic diseases by comparing the sensitivity and specificity of PCR after heat treatment using a microwave oven with a standard genomic DNA extraction method for paired serum and whole blood samples. The presence of serum IgM and...
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PMID: 20578507
PDF is available here.
PDF is available here.
Abstract
We suggest that this taxon is a new species, also within a new genus....
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PMID: 20487128
PDF is available here.
PDF is available here.
Le infezioni in medicina : rivista periodica di eziologia, epidemiologia, diagnostica, clinica e terapia delle patologie infettive (18)1 2010
Abstract
Malaria is one of the most important infectious diseases in the world. Although most cases occur in the tropical regions of Africa, Asia, Central and South America, there is in Europe a significant increase in the number of imported cases in non-endemic countries, in particular due to the higher mob...
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PMID: 20424521
PDF is available here.
PDF is available here.