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DNA, Recombinant (0):
Recent article
DNA, Recombinant
Xenobiotica (41)5 2011
Abstract
We have optimized the electroporation conditions in a large mammal model, i.e. pig. The parameters optimized include electric field intensity, number of pulses, lag time between plasmid injection and electroporation, and plasmid delivery volume. Constant current pulses, between 0.4 and 0.6 A, applie...
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PMID: 18370201
PDF is available here.
PDF is available here.
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Xenobiotica (41)5 2011
Abstract
Xenobiotica (41)5 2011
Abstract
We discuss that the DNA delivery into skin can be greatly enhanced by topical electroporation of the DNA injection site in rabbits using a tweezer electrode. Furthermore, the immune responses following a DNA vaccine delivery by using electroporation have been explored. Electroporation shows great po...
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PMID: 18370202
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
Using gene therapy to produce systemic levels of human factor IX for the treatment of hemophilia B has been clinically evaluated using viral-based vectors. The efficacy of this approach has been limited because of immune responses against the viral components. An alternative approach is to use physi...
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PMID: 18370215
PDF is available here.
PDF is available here.
Xenobiotica (41)5 2011
Abstract
The analysis of transgene inheritance is an important step in the molecular and genetic characterization of transgenes. In this manuscript, two approaches to characterize the inheritance of transgenes are described. The first approach is based on the expression of the transgene phenotype and the sec...
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PMID: 19378007
PDF is available here.
PDF is available here.
médecine/sciences (27)6-7 2011
Abstract
Abstract
We observe that on titration of ligand there are characteristic 'charge-state shifts' which manifest as changes in the relative intensities of protein peaks that correlate with the degree of protein-ligand complex formation. We suggest that use of this phenomenon will be particularly suitable for th...
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PMID: 21056549
PDF is available here.
PDF is available here.
Abstract
Two different endophytic strains, ESRAA1997 and ALAA2000, were isolated from the Egyptian herbal plant Anastatica hierochuntica. The 2 strains produced alkaline serine protease and were identified based on their phenotypic and chemotypic characteristics as different strains of Micromonospora spp. Bo...
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PMID: 20921985
PDF is available here.
PDF is available here.
Bioanalysis (2)7 2010
Abstract
I metabolism of drugs and poisons in humans, while UDP glycosyltransferases catalyze the majority of phase II reactions. In addition, a number of other enzymes or enzyme families contribute to the metabolism of xenobiotica, including alcohol dehydrogenase, aldehyde dehydrogenase, ester and amide hyd...
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PMID: 21083240
PDF is available here.
PDF is available here.
Abstract
We attempted to improve a radiation-responsive promoter of the p21 through a technique called DNA shuffling. A library of DNA fragments was constructed by re-ligation of randomly digested promoter fragments and improved promoters were chosen out of the library. We repeated this process twice to obta...
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PMID: 20541129
PDF is available here.
PDF is available here.
Abstract
Melanoma Research (20)3 2010
Abstract
I antigen upon intralesional administration. A phase 2 dose-escalation study (VCL-1005-208) was conducted to evaluate the safety and efficacy of Allovectin-7 in patients with metastatic melanoma. Eligible patients had stage III or IV metastatic melanoma recurrent or unresponsive to prior therapy, an...
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PMID: 20354459
PDF is available here.
PDF is available here.
Abstract
We describe the potential of microchip electrophoresis with a Hitachi SV1100, which can be used to determine DNA sizes between 500 and 5000 bp with good quantification (DNA concentration, <8 ng/l) within 5 min, for the analysis of DNA ligation. On analysis of an electropherogram of a ligation mixtur...
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PMID: 20196235
PDF is available here.
PDF is available here.
Abstract
We describe the results obtained in the ultrasensitive detection of non-amplified genomic DNA. We analyzed certified reference materials containing different amounts of genetically modified DNA by using a detection method which combines the nanoparticle-enhanced surface plasmon resonance imaging (SP...
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PMID: 20227870
PDF is available here.
PDF is available here.
Abstract
Our objective is to discuss the role velimogene aliplasmid (Allovectin-7, Vical Incorporated), a plasmid-lipid complex containing the DNA sequences encoding HLA-B7 and beta2 microglobulin, as an immunotherapeutic agent. WHAT THE READER WILL GAIN: Intralesional velimogene aliplasmid induces anti-tumo...
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PMID: 20367461
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (26)3 2010
Abstract
Adeno-associated virus (AAV) mediated RNA interference can be used to inhibit the expression of homologous genes in different mammalian cells. In this study, a transfer plasmid (pAAV-EGFP-H1) containing the H1 promoter and EGFP-expressing cassette was constructed based on the backbone of pAAV-MCS. U...
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PMID: 20572343
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (26)3 2010
Abstract
Our study identified the SINE2 gene was the determined gene for one way serological reaction between XJ-160 virus and SINV, and this research laid the foundation for further analysis of the genomic structure and function of SINV....
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PMID: 20572345
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (26)3 2010
Abstract
Based on the NSP4 sequence of bovine rotavirus (BRV), the shRNA was designed and synthesized, and a shRNA recombinant lenti-virus vector RNAi-H1-89 was constructed. The recombinant RNAi-H1-89 Lenti-virus was packaged by transfecting the 293T cell with the recombinant vector RNAi-H1-89 and two helper...
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PMID: 20572348
PDF is available here.
PDF is available here.
Abstract
The radiochemical purity of I/I-KH901 was over 95%. I-KH901 stimulated massive expression of granulocyte-macrophage colony-stimulating factor in tumor cells. Twenty-four hours after the addition of I-KH901, the concentrations of granulocyte-macrophage colony-stimulating factor were 183.27+/-6.90 and...
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PMID: 20147862
PDF is available here.
PDF is available here.
Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants and Agricultural Wastes (45)4 2010
Abstract
Composting was investigated as a means for safe disposal of organic waste containing bacteria that carry transgenes in recombinant plasmids. To generate model recombinant plasmids, a mobile IncQ plasmid, RSF1010, and a non-mobile plasmid, pGFP, were genetically modified to carry a DNA segment encodi...
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PMID: 20394128
PDF is available here.
PDF is available here.
Veterinary Microbiology (142)1-2 2010
Abstract
We have recently reported a full-genome amplification strategy for recovery of pestiviruses (Rasmussen et al., 2008). A full-length cDNA amplicon corresponding to the Border disease virus-Gifhorn genome was generated by long RT-PCR and then RNA transcripts derived from this amplicon were used to res...
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PMID: 19836906
PDF is available here.
PDF is available here.
Abstract
To observe the serum antibody response of the population, whose hepatitis B virus surface antigen(HBsAg), antibody to hepatitis B virus surface antigen (Anti-HBs), antibody to hepatitis B virus core antigen (Anti-HBc) are all negative to the different doses of Hepatitis B vaccine made by recombinant...
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PMID: 20575266
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (26)4 2010
Abstract
The 293FT cells infected by the recombinant lentivirus could express the fused protein SP-TAT-Apoptin. Anexin-V PI assay showed that SP-TAT-Apoptin carried by the recombinant lentivirus could cause the HepG2 cell apoptosis, and its apoptosis rate was significantly more than paired control group and...
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PMID: 20368104
PDF is available here.
PDF is available here.
Abstract
The male hypemethylated (MHM) region, associated with sex-specific DNA methylation and histone acetylation, encodes non-coding RNAs that are expressed exclusively on Z chromosome in female chickens. The function of the MHM non-coding RNAs is unclear yet. In order to analyze the regulation effect of...
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PMID: 19597752
PDF is available here.
PDF is available here.
Methods in Molecular Biology (633)633 2010
Abstract
The endocrine pancreas contains small clusters of 1,000-2,000 neuroendocrine cells termed islets of Langerhans. By secreting insulin, glucagon, or other hormones as circumstances dictate, islets play a central role in the control of glucose homeostasis in mammals. Islets are dispersed throughout the...
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PMID: 20204627
PDF is available here.
PDF is available here.
Molecular Biotechnology (44)2 2010
Abstract
Our results also demonstrated that the combination of these genes had a synergy effect in a CPC high producer....
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PMID: 19787461
PDF is available here.
PDF is available here.
Abstract
Two strains of the methylotrophic yeast Pichia pastoris were used to establish cyanophycin (multi-L-arginyl-poly-L-aspartic acid [CGP]) synthesis and to explore the applicability of this industrially widely used microorganism for the production of this polyamide. Therefore, the CGP synthetase gene f...
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PMID: 20038708
PDF is available here.
PDF is available here.
Abstract
We have eliminated these problems by using RASVs with regulated delayed expression of attenuation and regulated delayed synthesis of recombinant antigens. These changes result in RASVs that colonize effector lymphoid tissues efficiently to serve as "factories" to synthesize protective antigens that...
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PMID: 20370633
PDF is available here.
PDF is available here.
Abstract
We successfully used the guanidine isothiocyanate method for isolation of total RNA from leaf, stem, and root tissues of the aromatic plant Origanum onites. The RNA was extracted with TRI Reagent at room temperature and was recovered by isopropanol precipitation. The isolated RNA was capable of reve...
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PMID: 20469647
PDF is available here.
PDF is available here.
Cancer Biology & Therapy (8)22 2009
Abstract
Cancer Biology & Therapy (8)22 2009
Abstract
We evaluated the antitumor effect of IL-12 electrogene therapy (EGT) on murine SA-1 fibrosarcoma. The therapeutic plasmid was injected either intratumorally into subcutaneous SA-1 nodules or intradermally into the peritumoral region. We achieved a remarkable local and systemic antitumor effect with...
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PMID: 19755854
PDF is available here.
PDF is available here.
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (25)11 2009
Abstract
The recombinant retrovirus vector carrying LMP2A gene was constructed successfully. Transfection yield a titer of 5 x 10(8) infectious particles/L. The infected L929 cells were selected by Zeocine. Results of RT-PCR and Western blot indicated that L929 transgenetic cells could stably express EBV-LMP...
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PMID: 19900370
PDF is available here.
PDF is available here.
Abstract
Our findings suggest that self-regulation policies on recombinant DNA technology have influenced safety management for the life sciences and establishment of institutions for review in Japan....
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PMID: 19860031
PDF is available here.
PDF is available here.
Abstract
We will focus on the progress of these novel methods and strategies for strain improvement in recent years....
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PMID: 19777804
PDF is available here.
PDF is available here.
Gene Therapy (16)6 2009
Abstract
The last two years have seen significant advances in our understanding of the cellular innate responses elicited or activated by the entry of amplicon particles, which may, in part, explain the transient nature of transgene expression often observed in cells infected with helper-free amplicon stocks...
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PMID: 19369969
PDF is available here.
PDF is available here.
Veterinary Microbiology (137)1-2 2009
Abstract
We constructed a recombinant baculovirus that simultaneously expressed the genes for the P12A and 3C proteins of Asia I FMDV from individual promoters. The capsid proteins expressed in High Five insect cells were processed by viral 3C protease, as shown by Western blotting, and were antigenic, as re...
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PMID: 19167843
PDF is available here.
PDF is available here.
Abstract
We developed a practical recombinant DNA tool for multiple gene disruption in C. utilis based on the Cre-loxP system, which makes possible the reuse of selection markers. For this purpose, two plasmids were constructed: one harbored a heterologous loxP-flanked selection marker cassette carrying the...
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PMID: 19352042
PDF is available here.
PDF is available here.
Abstract
We assembled a cocktail vaccine comprising dozens of envelopes, delivered as recombinant DNA, vaccinia virus, and protein for testing in a clinical trial. One vaccinee has now completed vaccinations with no serious adverse events. Preliminary analyses demonstrate early proof-of-principle that a mult...
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PMID: 19241253
PDF is available here.
PDF is available here.
Abstract
Small-cell lung cancer (SCLC) is a highly malignant disease with poor prognosis. Hence, there is great demand for new therapies that can replace or supplement the current available treatment regimes. Gene therapy constitutes a promising strategy and relies on the principle of introducing exogenous D...
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PMID: 19374598
PDF is available here.
PDF is available here.
Abstract
Recent research has begun to elucidate the global network of cytosolic and membrane protein interactions. The resulting interactome map facilitates numerous biological studies, including those for cell signalling, protein trafficking and protein regulation. Due to the hydrophobic nature of membrane...
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PMID: 19521829
PDF is available here.
PDF is available here.
Bioscience Reports (29)2 2009
Abstract
We studied the feasibility of deleting essential genes in insect cells by using bacmid and purifying recombinant bacmid in Escherichia coli DH10B cells. To disrupt the orf4 (open reading frame 4) gene of BmNPV [Bm (Bombyx mori) nuclear polyhedrosis virus], a transfer vector was constructed and co-tr...
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PMID: 18684105
PDF is available here.
PDF is available here.
Abstract
Comprehensive analyses on promoters of four cellulase and one xylanase genes of Trichoderma reesei were performed expressing a single reporter uidA from Escherichia coli to construct highly functional cellulase-overproducing strains. GUS amount expressed under each promoter correlated entirely with...
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PMID: 19148637
PDF is available here.
PDF is available here.
Parasitology (94 ( Pt 2))02 2009
Abstract
A small, size selected (0.5-5.0 Kbp) genomic DNA library has been constructed in the bacterial plasmid pAT153 using DNA extracted from a human isolate (Kenyan origin) of the hydatid disease organism, Echinococcus granulosus. A panel of taeniid cestode DNAs has been used in conjunction with hybridiza...
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PMID: 3035464
PDF is available here.
PDF is available here.
Abstract
Genetically modified animals have been used as models in broad range of studies including pharmaceutical biology. Designing and construction of transgene constructs are the first indispensable task in generating model animals. In addition to the classical restriction enzyme-based method, still holds...
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PMID: 19199958
PDF is available here.
PDF is available here.
Tree Physiology (29)2 2009
Abstract
We quantified expression of two reporter genes, green fluorescent protein (GFP) and the herbicide bialaphos resistance gene (BAR), in 2256 transgenic poplar trees derived from 404 primary events, and in 106 in vitro-redifferentiated subevents, over 3 years in the greenhouse and in the field. No gene...
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PMID: 19203955
PDF is available here.
PDF is available here.
Abstract
There has been rapid progress in recent years in extending gene transfer capabilities to include plant species that fall outside the normal host range of Agrobacterium. Methods that allow direct DNA delivery into plant cells have contributed significantly to this expanded capability. Whiskers treatm...
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PMID: 19378002
PDF is available here.
PDF is available here.
Abstract
We developed a novel type of real-time polymerase chain reaction (PCR) array with TaqMan chemistry as a platform for the comprehensive and semiquantitative detection of genetically modified (GM) crops. Thirty primer-probe sets for the specific detection of GM lines, recombinant DNA (r-DNA) segments,...
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PMID: 19072282
PDF is available here.
PDF is available here.
BMC Biotechnology (9)1 2009
Abstract
In both episomal and chromosomal assays, DNA fragments were more efficient at gene repair than oligonucleotides or rAAV-1. Furthermore, the gene targeting frequency could be significantly increased by using DNA repair stimulating drugs such as doxorubicin and phleomycin. CONCLUSION: Our results show...
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PMID: 19379497
PDF is available here.
PDF is available here.
Biostatistics (10)1 2009
Abstract
We introduce a novel random tips (RT) model. The model springs from the idea that a recombinant sequence inherits its characters from an unknown number of ancestral full-length sequences, of which one only observes the incomplete portions. The RT model decomposes recombinant sequences into their anc...
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PMID: 18562348
PDF is available here.
PDF is available here.
Abstract
The expression and secretion of hCT were successfully detected in pCDNA3.0-Igk-hCT-transfected NIH3T3 cells. The number of osteoclasts was decreased and the cells became crumpled when they were incubated with the supernatant of pCDNA3.0-Igk-hCT-transfected NIH3T3 cells. CONCLUSION: A recombinant euk...
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PMID: 19696904
PDF is available here.
PDF is available here.
Abstract
We amplified this gene from the H37Rv genome, and His-tagged Rv0901 protein was expressed and purified. Also, a recombinant plasmid bearing Rv0901 was constructed and electroporated into a virulent Mycobacterium smegmatis, using shuttle expression vector pMV261. Transformants were induced to express...
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PMID: 19168955
PDF is available here.
PDF is available here.
Abstract
In order to develop practical recombinant DNA techniques in the industrially important yeast Candida utilis, at least six plasmids harboring autonomously replicating sequences (ARSs) were isolated from a C. utilis genomic library. Two ARSs were subjected to detailed analysis. Sequences of 1.9 and 1....
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PMID: 19129631
PDF is available here.
PDF is available here.
Abstract
We describe a detailed protocol for genetic transformation of the maize genotype, Hi II. The protocol uses 0.6-microm gold particles as microcarriers and the herbicide resistance bar gene as a selective marker. Both immature zygotic embryos and immature embryo-derived callus cultures can be transfor...
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PMID: 19378004
PDF is available here.
PDF is available here.
Abstract
We describe here the application of this technique in the fission yeast, Schizosaccharomyces pombe....
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PMID: 19563124
PDF is available here.
PDF is available here.
Abstract
Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the product...
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PMID: 19347644
PDF is available here.
PDF is available here.
Abstract
We focus here on DNA delivery by particle bombardment as Agrobacterium-mediated transformation methods for wheat are reported elsewhere....
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PMID: 19347645
PDF is available here.
PDF is available here.
Abstract
Gene transfer methods for producing recombinant cell lines are often not very efficient. One reason is that the recombinant DNA is delivered into the cell cytoplasm and only a small fraction reaches the nucleus. This chapter describes a method for microinjecting DNA directly into the nucleus. Direct...
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PMID: 19085129
PDF is available here.
PDF is available here.
Abstract
The following method describes the generation of a recombinant vaccinia virus expressing a costimulatory molecule (human CD80 or B7.1).The procedure first requires the cloning, by classical methods not described here, of the gene of interest, e.g. CD80, into a vaccinia shuttle plasmid under the cont...
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PMID: 19048219
PDF is available here.
PDF is available here.
FASEB Journal (23)1 2009
Abstract