Abstract
We show that the replication-associated proteins (Rep: a rolling-circle replication initiator protein) encoded by these alphasatellites interact with the Rep and C4 proteins encoded by their helper begomovirus, Cotton leaf curl Rajasthan virus (CLCuRaV), in a yeast two-hybrid assay. Both the alphasa...
|
PMID: 20598726
PDF is available here.
Abstract
The search for all sequences containing centromeric (CEN) minor satellite (MiSat) or pericentromeric (peri-CEN) mouse major satellite (MaSat) was conducted in the whole genome shotgun (WGS) database. The sequences were checked for the presence of the known dispersed repeats using the Censor software...
|
PMID: 21061622
PDF is available here.
Abstract
DNA clones containing highly repetitive DNA sequences were selected from partial libraries of Lathyrus sativus and L. sylvestris. Two satellite DNA sequence families were isolated from the genome of the former species. A first family was made up of repeats that varied in length from 54-56 bp, and sh...
|
PMID: 20424423
PDF is available here.
Abstract
Nuclear stress bodies (nSBs) are unique subnuclear organelles which form in response to heat shock. They are initiated through a direct interaction between heat shock transcription factor 1 (HSF1) and pericentric tandem repeats of satellite III sequences and correspond to active transcription sites f...
|
PMID: 20516127
PDF is available here.
Abstract
We show that parental 'Watson' and 'Crick' DNA template strands can be identified in sister chromatids of murine metaphase chromosomes using CO-FISH (chromosome orientation fluorescence in situ hybridization) with unidirectional probes specific for centromeric and telomeric repeats. All chromosomes...
|
PMID: 20016487
PDF is available here.
Abstract
We investigated, by chromatin immunoprecipitation, immunofluorescence, fluorescence in situ hybridization, and confocal microscopy, the capacity of NSs to interact with the host genome. Our results demonstrate that even though cellular DNA is predominantly excluded from NSs filaments, NSs interacts...
|
PMID: 19889787
PDF is available here.
Abstract
Genetic variety estimation of hop gene pool using DNA-typing of highly polymorphic microsatellite loci and optimization of introduction to the culture of in vitro conditions is the important stage of national varieties resources forming, basis of modern nursery and protect mean of varieties property...
|
PMID: 21061686
PDF is available here.
Abstract
We demonstrate that both Y10 DNA beta (Y10beta) and Y35 DNA beta (Y35beta) were stably trans-replicated by the noncognate Begomovirus, although the noncognate DNA beta accumulated in plants at a lower level than did the cognate DNA beta. In Nicotiana benthamiana and N. glutinosa plants agroinoculate...
|
PMID: 19453231
PDF is available here.
Abstract
We examined ovarian epithelial tumors and diverse control tissues for methylation on only one strand (hemimethylation), both strands (symmetrical methylation), or neither strand at Sat2 CpG dyads using hairpin genomic sequencing. Analysis of the resulting cloned DNA molecules indicated that although...
|
PMID: 19287214
PDF is available here.
Abstract
We report on an ex-ovo system using axolotl oocyte extracts to remodel epigenetic marks of somatic chromatin. Molecules present in axolotl oocyte extracts induce the reduction of the overall levels of H3K9me3, HP1alpha and DNA methylation of somatic cells, and they increase the levels of H3K9ac. The...
|
PMID: 19440040
PDF is available here.
Abstract
Although extant data favour centromere being an epigenetic structure, it is also clear that centromere formation is based on DNA, in particular, tandemly repeated satellite DNA and its transcripts. Presence of conserved structural motifs within satellite DNAs such as periodically distributed AT trac...
|
PMID: 19521812
PDF is available here.
Abstract
We present an algorithm that remedies these shortcomings: our algorithm is alphabet-independent and is based on the run-length encoding scheme. It is the fastest in theory, and in practice as well, as shown by experimental results. Furthermore, our alignment model is more general than that of the pr...
|
PMID: 19340916
PDF is available here.
Abstract
We have used the model plant Rumex acetosa, a dioecious species with multiple sex chromosomes, 2n = 12 + XX female and 2n = 12 + XY(1)Y(2) male, to analyse the significance of repetitive DNA accumulation during the differentiation of the Y. A bulk segregant analysis (BSA) approach allowed us to iden...
|
PMID: 19085011
PDF is available here.
Abstract
We determined the localization of 4 types of mouse satDNA CEN and periCEN regions and associated proteins: RNA-helicase p68, SMC3, Rad21 subunits of the cohesin complex and SYCP3 subunit of the synaptonemal complex (SC). Partially flattened nuclei of the one- and two-cell embryos and embryos treated...
|
PMID: 19566037
PDF is available here.
Abstract
A hitherto undescribed satellite DNA family (AvaII satDNA) has been isolated and characterised in Eumeces schneideri, a squamate reptile belonging to the family Scincidae. AvaII satDNA is characterised by a monomer length of 208 bp, a GC content of 59% and exhibits a certain degree of CpG methylatio...
|
PMID: 19864890
PDF is available here.
Abstract
Karyotype and chromosomal characteristics from 3 allopatric populations of Hoplias malabaricus, cytogenetically the most studied Erythrinidae taxon, were investigated using different staining techniques (C-, Ag-, and CMA(3) banding) as well as fluorescent in situ hybridization (FISH) to detect 18S r...
|
PMID: 19729917
PDF is available here.
Abstract
We microdissected X and B chromosomes and amplified the obtained DNA by 2 different procedures, the conventional DOP-PCR method and the single-cell whole-genome amplification GenomePlex method. We then generated DNA probes to perform chromosome painting. Our results have confirmed that X and B chrom...
|
PMID: 19864892
PDF is available here.
Abstract
We will focus on the evidences that associate satellite DNAs and constitutive heterochromatin to the process of chromosome evolution and consequently to domestic species genome's remodeling.
(c) 2009 S. Karger AG, Basel....
|
PMID: 20016153
PDF is available here.
Abstract
We hypothesized that the chromosome 9 pericentromeric DNA conserved in human chromosomes and deriving from the gorilla genome may thus play some important functional role.
Copyright 2009 S. Karger AG, Basel....
|
PMID: 20068296
PDF is available here.
Abstract
A new highly abundant satellite DNA from Discoglossus pictus (Dp-sat1) was isolated and characterized. The repetitive unit (0.51 kb) has 2 HindIII sites and only one SpeI site: digestion of genomic DNA with HindIII produces 3 fragments: HA (0.17 kb), HB (0.34 kb), and HC = HA + HB (0.51 kb), while d...
|
PMID: 20110657
PDF is available here.
Abstract
We have constructed a physical map and produced experimental patterns of mouse gamma-satellite DNA hydrolysis with unique site-specific methyl-directed endonuclease GlaI and several restriction endonucleases. Fifty two DNA fragments of gamma-satellite DNA have been cloned and sequenced. We have not...
|
PMID: 19607729
PDF is available here.
Abstract
FISH analysis revealed that the satellite repeat showing homology with intergenic spacer (IGS) of rDNA present in the tomato genome. By comparing the sequences representing distinct stages in the divergence of rDNA repeat with those of canonical rDNA arrays, the molecular mechanism of the evolution...
|
PMID: 19351415
PDF is available here.
Abstract
We found 2 individuals with unexpected breaks in the centromeric alpha-satellite DNA. Subsequently, centromere breaks were specifically sought in reciprocal translocations in which 1 or both breakpoints appeared cytogenetically near the centromere. In a total of 11 individuals studied, we found 8 ce...
|
PMID: 19864896
PDF is available here.
Abstract
Our results indicate that interaction of the G-box motif with host nuclear factors is important for efficient gene expression and replication of DNA beta....
|
PMID: 19088296
PDF is available here.
Abstract
The study of the degree of DNA methylation in the nucleus, in particular of the major satellite in two-cell mouse embryos developing in the maternal organism, in standard cultural media M16 used for cultivation of mouse embryos and M2 media used for manipulations with embryos in the air was conducte...
|
PMID: 19894612
PDF is available here.
Abstract
We investigated the interaction of TYLCV-IL[JR:Toc:07] with two known satellites associated with tomato yellow dwarf disease in Japan, tobacco leaf curl Japan betasatellite [Japan:Ibaraki:2006] and honeysuckle yellow vein mosaic betasatellite [Japan:Nara:2006], as well as with tomato leaf curl Phili...
|
PMID: 19575277
PDF is available here.
Abstract
We have demonstrated that HYVV is unable to trans-replicate other satellites. To investigate the basis of trans-replication compatibility, deletion mutagenesis of AYVV DNA-beta has been used to localize the origin of replication to approximately 360 nt, encompassing the ubiquitous nonanucleotide/ste...
|
PMID: 19008407
PDF is available here.
Abstract
We isolated variants from both the Mc1 and Ac2 pig (Sus scrofa, Suidae) satellite DNA families from the genomes of two Tayassuidae members: Pecari tajacu and Tayassu pecari, which have highly derived karyotypes. The presence of these sequences in both families' genomes (Suidae and Tayassuidae) impli...
|
PMID: 18440236
PDF is available here.
Abstract
We report the isolation of Cricetus cricetus (CCR, Cricetidae, Rodentia) centromeric repetitive sequences from chromosome 4 (CCR4/10sat), using the laser microdissection and laser pressure catapulting procedure, followed by DOP-PCR amplification and labelling. Physical mapping by fluorescent in situ...
|
PMID: 18602266
PDF is available here.
Abstract
We identify KDM2A, a JmjC-domain containing histone demethylase, as a heterochromatin-associated and HP1-interacting protein that promotes HP1 localization to chromatin. We show that KDM2A is required to maintain the heterochromatic state, as determined using a candidate-based approach coupled to an...
|
PMID: 19001877
PDF is available here.
Abstract
Short arms, satellite stalks and satellites of human acrocentric chromosomes (13, 14, 15, 21 and 22) represent heterochromatic regions. Enforced by mutual attraction of heterochromatic regions, the short arms of acrocentric chromosomes come close to each other and compose associations. The associati...
|
PMID: 19075353
PDF is available here.
Abstract
The complete nucleotide sequences of two begomoviruses (Nara virus-1 and Nara virus-2), a satellite DNA (DNAbeta-Nara) and defective DNAs were obtained from honeysuckle (Lonicera japonica) showing characteristic yellow vein mosaic symptoms in Nara Prefecture, Japan. One begomovirus (Ibaraki virus) a...
|
PMID: 18722488
PDF is available here.
Abstract
We discovered a mariner-family transposon, Mboumar, in the satellite DNA of the ant Messor bouvieri. Several copies of the transposon contain a full-length open reading frame, including Mboumar-9, which has 64% nucleotide identity to Mos1 of Drosophila mauritiana. To determine whether Mboumar is cur...
|
PMID: 18675277
PDF is available here.
Abstract
We have developed molecular cytogenetic markers for 13 Citrus species collected from Jeju island, Korea. In this study, we demonstrated that the chromosomal locations of cytogenetic markers are quite variable and extremely polymorphic, in contrast to the previous studies. The data obtained in this s...
|
PMID: 18566546
PDF is available here.
Abstract
The complexity of the pBV1 satellite family observed in the section Beta of the genus Beta and, in particular, the strong amplification of the pBV1/pRv1 satellite in the domesticated B. vulgaris indicates the dynamics of centromeric satellite evolution during species radiation within the genus. The...
|
PMID: 18682437
PDF is available here.
Abstract
Our data shows that Mexican cattle populations have a relatively high level of genetic diversity based either on the mean number of alleles (10.2-13.6) and on the expected heterozygosity (0.71-0.85). The degree of observed homozygosity within the Criollo populations was remarkable and probably cause...
|
PMID: 18803791
PDF is available here.
Abstract
We have analysed these plants for evidence of the hallmarks of silencing. The GUS transcript was not detectable in the leaves of GUS-silenced tobacco plants. These plants contained siRNAs of approximately 23 nt in length homologous to both the 35S promoter region and the GUS ORF. The absence of GUS...
|
PMID: 18514962
PDF is available here.
M Akiyama,
K Yatsu,
M Ota,
Y Katsuyama,
K Kashiwagi,
F Mabuchi,
H Iijima,
K Kawase,
T Yamamoto,
M Nakamura,
A Negi,
T Sagara,
N Kumagai,
T Nishida,
M Inatani,
H Tanihara,
S Ohno,
H Inoko and
N Mizuki
Abstract
Fifteen microsatellite markers within and/or near to the GLC1B locus were genotyped, and their association with normal tension glaucoma was analysed. Two markers D2S2264 and D2S176 had significant positive associations. CONCLUSION: The D2S176 marker had the strongest significant association and it i...
|
PMID: 18723748
PDF is available here.
Abstract
Three clones containing satellite DNA sequences were selected from a randomly sheared genomic DNA library of Picea abies (clones PAF1, PAG004P22F (2F), and PAG004E03C (3C)). PAF1 contained 7 repeats that were 37-55 bp in length and had 68.9%-91.9% nucleotide sequence similarity. Two 2F repeats were...
|
PMID: 18772948
PDF is available here.
Abstract
I report that on the active X chromosome (Xa), DXZ4 is organized into constitutive heterochromatin characterized by a highly organized pattern of H3K9me3. DXZ4 is expressed from both strands and generates an antisense transcript that is processed into small RNAs that directly correlate with H3K9me3...
|
PMID: 18456864
PDF is available here.
Abstract
We show here that SalI is also found in H. fulgens and is not useful for screening hybrid individuals bred in aquaculture or occurring naturally in the wild. SalI is a family of predominantly subtelomeric tandemly repeated sequences, and sequenced clones revealed clustering to species and little int...
|
PMID: 18650947
PDF is available here.
Abstract
We demonstrate that the N- and C-terminal domains of a nucleolar protein, hNopp140, bound respectively to alpha-satellite arrays and rDNA clusters of acrocentric chromosomes for nucleolar formation. The central acidic-and-basic repeated domain of hNopp140, possessing a weak self-self interacting abi...
|
PMID: 18253863
PDF is available here.
Abstract
We show by RNA blotting and RT-PCR that 195 SAT is intensely transcribed. We observed a positive correlation between the level of satellite RNA and the abundance of the satellite copies in the genome of T. cruzi strains and that the satellite expression is not developmentally regulated. By analyzing...
|
PMID: 18440654
PDF is available here.
Abstract
We use a chromosome orientation fluorescence in situ hybridization technique to visualize and quantify recombination events at mouse centromeres. We show that centromere mitotic recombination occurs in normal cells to a higher frequency than telomere recombination and to a much higher frequency than...
|
PMID: 18541703
PDF is available here.
Abstract
We examined the Trypanosoma cruzi genome for histone modification patterns shown to be linked to active genes in various organisms. Here, we show that acetylated and methylated histones were found to be enriched at strand switch regions of divergent gene arrays, not at convergent clusters or intra-...
|
PMID: 18400752
PDF is available here.
Abstract
These results suggest that ALSs are a transient structural feature in the cells of any organisms and point to a non-universal model of chromatin organization in sperm cells among mammals....
|
PMID: 17651406
PDF is available here.
Abstract
We found high within-host virulence but low transmission rates in a clone of social parasitic A. m. capensis workers invading the neighbouring subspecies A. m. scutellata. In contrast, parasitic workers from the endemic range of A. m. capensis showed low within-host virulence but high transmission r...
|
PMID: 18288468
PDF is available here.
Abstract
The study of insect satellite DNAs (satDNAs) indicates the evolutionary conservation of certain features despite their sequence heterogeneity. Such features can include total length, monomer length, motifs, particular regions and/or secondary and tertiary structures. satDNAs may act as protein-bindi...
|
PMID: 18414505
PDF is available here.
Abstract
We report on an efficient VIGS system that functions in tomato roots using a modified viral satellite DNA (DNAmbeta) associated with Tomato yellow leaf curl China virus (TYLCCNV). A cDNA fragment of the ferric chelate reductase gene (FROl) from tomato was inserted into the DNAmbeta vector. Tomato ro...
|
PMID: 18785585
PDF is available here.
Abstract
Genomes of higher eukaryotes contain abundant non-coding repeated sequences whose overall biological impact is unclear. They comprise two categories. The first consists of retrotransposon-derived elements. These are three major families of retroelements (LINEs, SINEs and LTRs). SINEs are clustered i...
|
PMID: 18348251
PDF is available here.
Jose Roman-Gomez,
Antonio Jimenez-Velasco,
Xabier Agirre,
Juan A Castillejo,
German Navarro,
Edurne San Jose-Eneriz,
Leire Garate,
Lucia Cordeu,
Francisco Cervantes,
Felipe Prosper,
Anabel Heiniger and
Antonio Torres
Abstract
We investigated methylation changes of DNA repetitive elements (LINE1, Alu, Satellite-alpha and Satellite-2) during the progression of CML from chronic phase (CP) to blast crisis (BC). CP-CML samples were significantly more hypomethylated for all repetitive sequences compared with normal samples. Fu...
|
PMID: 17765966
PDF is available here.
Abstract
We describe for the first time the karyotype of A. oxyrinchus (2n = 121 +/- 3), and its features, among which the constitutive heterochromatin, revealed by C-banding technique, the distribution of telomeric regions, and the 5S rRNA genes, detected by FISH. The results reveal that A. oxyrinchus and A...
|
PMID: 17624498
PDF is available here.
Abstract
Reverse transcriptase-polymerase chain reaction and immunohistochemical analyses revealed human amylase mRNA and protein expression, respectively, in the pancreata from recipient mice. Using fluorescence in situ hybridization, we identified human alpha-satellite, DNA-positive cells with a morphology...
|
PMID: 18376297
PDF is available here.
Abstract
We discovered two new complex elements while studying large genomic rearrangements and segmental duplications in the human genome. Both resemble bacterial composite DNA transposon Tn9, consisting of a core flanked by mobile elements, except that the flanking element is not a DNA transposon but inste...
|
PMID: 18083327
PDF is available here.
Abstract
We report the occurrence of eccDNA in small and large genome plant species, as identified using two-dimensional gel electrophoresis. We show that eccDNA is readily detected in both Arabidopsis thaliana and Brachycome dichromosomatica, reflecting a normal phenomenon that occurs in wild-type plants. T...
|
PMID: 18088310
PDF is available here.
Abstract
We argue that tandem repeats may be advantageous forms of DNA sequences in (peri)centromeres due to concerted evolution, which maintains high intra-array and intrapopulation sequence homogeneity of satellite arrays, while allowing rapid changes in nucleotide sequence and/or composition of satellite...
|
PMID: 18182173
PDF is available here.
Abstract
We use real-time RT-PCR to study the expression of these genomic regions. Transcription is highly asymmetrical and most of the transcripts contain the G-rich strand of the repeat. A low level of G-rich RNAs is detectable in unstressed cells and a 10(4)-fold induction occurs after heat shock. G-rich...
|
PMID: 18039709
PDF is available here.
M Fanelli,
S Caprodossi,
L Ricci-Vitiani,
A Porcellini,
F Tomassoni-Ardori,
S Amatori,
F Andreoni,
M Magnani,
R De Maria,
A Santoni,
S Minucci and
P G Pelicci
Abstract
We report that human glioblastoma is affected by strong hypomethylation of satellite 2 pericentromeric sequences that involves the stem cell compartment. Concomitantly with the integrity of the DNMTs coding sequences, we report aberrations in DNA methyltrasferases expression showing upregulation of...
|
PMID: 17653095
PDF is available here.