Abstract
We show that selective autophagy downregulates Ty1 transposition by eliminating Ty1 VLPs from the cytoplasm under nutrient-limited conditions. Ty1 VLPs are targeted to autophagosomes by an interaction with Atg19. We propose that selective autophagy safeguards genome integrity against excessive inser...
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PMID: 21839922
PDF is available here.
Abstract
We found that introduction of Ty912, a previously identified Ty1 element, onto the non-essential terminal region of the left arm of chromosome V led to a 380-fold increase in the rate of accumulating GCRs in a wild-type strain. A survey of 48 different mutations identified those that either increase...
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PMID: 21637792
PDF is available here.
Toshiaki Watanabe,
Shinichiro Chuma,
Yasuhiro Yamamoto,
Satomi Kuramochi-Miyagawa,
Yasushi Totoki,
Atsushi Toyoda,
Yuko Hoki,
Asao Fujiyama,
Tatsuhiro Shibata,
Takashi Sado,
Toshiaki Noce,
Toru Nakano,
Norio Nakatsuji,
Haifan Lin and
Hiroyuki Sasaki
Abstract
We generated Mitopld mutant mice. The mice display meiotic arrest during spermatogenesis, demethylation and derepression of retrotransposons, and defects in primary piRNA biogenesis. Furthermore, in mutant germ cells, mitochondria and the components of the nuage, a perinuclear structure involved in...
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PMID: 21397847
PDF is available here.
Abstract
We firstly found that TRIM5-CypA fusion gene exist in M. assamensis. The TRIMCyp of M. assamensis also results from the retrotransposition of CypA pseudogene cDNA into 3'-UTR of TRIM5 gene like TRIMCyp of M. leonina. Moreover, there is an extremely high sequence homology between TRIMCyp genes from M...
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PMID: 21341383
PDF is available here.
Abstract
This article reviews the history of widespread exchanges of genetic segments initiated over 3 billion years ago, to be part of their life style, by sphero-protoplastic cells, the ancestors of archaea, prokaryota, and eukaryota. These primordial cells shared a hostile anaerobic and overheated environm...
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PMID: 21506007
PDF is available here.
Abstract
We speculate that intrastrain transposition of the three retroelements studied is controlled predominantly at the post-transcriptional level....
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PMID: 21779346
PDF is available here.
Abstract
Transposons and retrotransposons represent a great portion of the eukaryotic genome. These repeated elements can be defined as genetic entities capable of self-inserting into different positions within the genome modifying the functions of the genes with which they are associated. The purpose of the...
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PMID: 20798486
PDF is available here.
Abstract
We lately discovered endogenous elements homologous to the nucleoprotein of bornaviruses, a negative-strand RNA virus, in the genomes of many mammalian species, including humans. We also demonstrated that mRNA of extant mammalian bornavirus, Borna disease virus, is reverse-transcribed and integrated...
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PMID: 21488328
PDF is available here.
Abstract
We show that zebrafish hen1 is specifically expressed in germ cells and is essential for maintaining a female germ line, whereas it is dispensable in the testis. Hen1 protein localizes to nuage through its C-terminal domain, but is not required for nuage formation. In hen1 mutant testes, piRNAs beco...
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PMID: 20859253
PDF is available here.
Abstract
We investigated role of LTR retrotransposons in expression of genes in Drosophila. Using database of spliced ESTs we identified only 13 potential cases of transcription initiation from long terminal repeats of LTR within euchromatic part of the Drosophila genome. Our results indicate on insignifican...
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PMID: 21061618
PDF is available here.
Abstract
The search for all sequences containing centromeric (CEN) minor satellite (MiSat) or pericentromeric (peri-CEN) mouse major satellite (MaSat) was conducted in the whole genome shotgun (WGS) database. The sequences were checked for the presence of the known dispersed repeats using the Censor software...
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PMID: 21061622
PDF is available here.
Abstract
We show that dosage compensation (DC) in Drosophila may contribute substantially to the depletion of male genes on the X. To equalize expression between X-linked and autosomal genes in the two sexes, male Drosophila hypertranscribe their single X, whereas female mammals silence one of their two X ch...
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PMID: 20705467
PDF is available here.
Abstract
We have therefore developed a new biodosimetric technique targeting circulating B1 DNA in mouse plasma by branched DNA signal amplification for rapid quantification of plasma DNA. This technology targets repetitive elements of the B1 retrotransposon in the mouse genome, followed by signal amplificat...
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PMID: 20675464
PDF is available here.
Abstract
We have also introduced Penelope into Drosophila melanogaster, which are otherwise naive to the element. We have taken advantage of these natural and experimentally induced colonization processes to probe the evolution of small RNA pathways in response to transposon challenge. In both species, Penel...
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PMID: 20581131
PDF is available here.
Abstract
Using cosmid vector, a gene library of German cockroach Blattella germanica was constructed. From this library, clones containing full-length copies of two subfamilies of R1 retroposons were selected. Retroposons R1 of German cockroach belonging to different subfamilies were shown to be different in...
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PMID: 20873200
PDF is available here.
Abstract
We show that an LTR retrotransposon of ERV-9 human endogenous retrovirus located 40-70 kb upstream of the human fetal gamma- and adult beta-globin genes serves a long-range, host function. The ERV-9 LTR contains multiple CCAAT and GATA motifs and competitively recruits a high concentration of NF-Y a...
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PMID: 20615953
PDF is available here.
Abstract
We have previously shown that the maize (Zea mays subsp. mays) retrotransposon Bs1 has transduced sequences from three different host genes. Here, we provide evidence that these transduction events led to the generation of a chimeric new gene that is both transcribed and translated. Expression of Bs...
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PMID: 20488894
PDF is available here.
Abstract
We have isolated a new Ty1-copia-like retrotransposon, named Ttd1a from the Triticum durum L. genome. To get insight into stress activation pathways in Ttd1a, we investigated the effect of salt and light stresses by RT-PCR and S-SAP profiling. We screened for Ttd1a insertion polymorphisms in plants...
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PMID: 20237753
PDF is available here.
Abstract
We studied the 12 rice (Oryza sativa) homologs of Su(var)3-9, the histone H3 lysine 9 (H3K9) methyltransferase identified in Drosophila. Several rice SUVHs (i.e. SDG714, SDG727, and SDG710) were found to have an antagonistic function to the histone H3K9 demethylase JMJ706, as down-regulation of thes...
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PMID: 20566579
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
|
PMID: 20408998
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
The 35S ribosomal DNA (rDNA) intergenic spacer (IGS) of Allium cernuum is examined. Initial sequencing of IGS clones revealed that some rDNA units contain a truncated retrotransposon sequence most similar to members of the Copia superfamily. Fluorescence in situ hybridisation (FISH) to metaphase chr...
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PMID: 20516662
PDF is available here.
Jason E Stajich,
Sarah K Wilke,
Dag Ahrén,
Chun Hang Au,
Bruce W Birren,
Mark Borodovsky,
Claire Burns,
Björn Canbäck,
Lorna A Casselton,
C K Cheng,
Jixin Deng,
Fred S Dietrich,
David C Fargo,
Mark L Farman,
Allen C Gathman,
Jonathan Goldberg,
Roderic Guigó,
Patrick J Hoegger,
James B Hooker,
Ashleigh Huggins,
Timothy Y James,
Takashi Kamada,
Sreedhar Kilaru,
Chinnapa Kodira,
Ursula Kües,
Doris Kupfer,
H S Kwan,
Alexandre Lomsadze,
Weixi Li,
Walt W Lilly,
Li-Jun Ma,
Aaron J Mackey,
Gerard Manning,
Francis Martin,
Hajime Muraguchi,
Donald O Natvig,
Heather Palmerini,
Marilee A Ramesh,
Cathy J Rehmeyer,
Bruce A Roe,
Narmada Shenoy,
Mario Stanke,
Vardges Ter-Hovhannisyan,
Anders Tunlid,
Rajesh Velagapudi,
Todd J Vision,
Qiandong Zeng,
Miriam E Zolan and
Patricia J Pukkila
Abstract
The mushroom Coprinopsis cinerea is a classic experimental model for multicellular development in fungi because it grows on defined media, completes its life cycle in 2 weeks, produces some 10(8) synchronized meiocytes, and can be manipulated at all stages in development by mutation and transformati...
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PMID: 20547848
PDF is available here.
Abstract
We demonstrated on a genomic scale the localization of a DNA-turnover marker [5-bromo-2'-deoxyuridine (BrdU); an analogue of thymidine], indicative of DNA synthesis in discrete, multiple sites across the genome. Exponential-phase cells on the other hand, exhibited a uniform, total genomic DNA synthe...
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PMID: 20167621
PDF is available here.
Abstract
We find that circularization of the HIV-1 genome does not affect the initiation of reverse transcription, but stimulates the first strand transfer during reverse transcription in vitro, underscoring the functional importance of the interaction....
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PMID: 20430859
PDF is available here.
Abstract
We investigated the effect of VL30 retrotransposition on cell death in SV40-transformed mouse SVTT1 cells. Transfection of a VL30 retrotransposon decreased the clonogenicity of SVTT1 by 17-fold, as compared to parental NIH3T3 cells. Correlated levels of retrotransposition frequency and cell death ra...
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PMID: 20386572
PDF is available here.
Abstract
We consider the range and potentials of the various mechanisms, focusing on the cut-and-paste transposons as one of the more promising avenues towards gene therapy applications. Several cut-and-paste transposition systems are currently under development. We will first consider the mechanisms of pigg...
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PMID: 19649713
PDF is available here.
Abstract
Tc1, one of the founding members of the Tc1/mariner transposon superfamily, was identified in the nematode Caenorhabditis elegans more than 25 years ago. Over the years, Tc1 and other endogenous mariner transposons became valuable tools for mutagenesis and targeted gene inactivation in C. elegans. H...
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PMID: 19347589
PDF is available here.
Abstract
In the last 20 years, tools derived from DNA transposons have made major contributions to genetic studies from gene delivery to gene discovery. Various complementary and fairly ubiquitous DNA vehicles have been developed. Although many transposons are efficient DNA vehicles, they appear to have limi...
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PMID: 19662501
PDF is available here.
Abstract
Mariners are small DNA mediated transposons of eukaryotes that fortuitously function in bacteria. Using bacterial genetics, it is possible to study a variety of properties of mariners, including transpositional ability, dominant-negative regulation, overexpresson inhibition, and the function of cis-...
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PMID: 19711186
PDF is available here.
Abstract
We screened a Tnt1 retrotransposon insertion mutant population for plants with altered patterns of lignin autofluorescence. From more than 9000 R1 plants screened, four independent lines were identified with a total lack of lignin in the interfascicular region. The mutants also showed loss of lignin...
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PMID: 20408998
PDF is available here.
Abstract
The genomes of five species belonging to the grass family have been fully sequenced and provide insight into the structural organization and evolution of grass genomes. Comparative analyses have shown that genes and repeats are organized differently in small genomes compared to large genomes. Small...
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PMID: 20064738
PDF is available here.
Abstract
Retrotransposons constitute a major proportion of the Triticeae genomes. Genome-scale studies have revealed their role in evolution affecting both genome structure and function and their potential for the development of novel markers. In this study, family members of an LTR copia ret...
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PMID: 20127492
PDF is available here.