Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Oligonucleotide Probes (0):
Recent article
Oligonucleotide Probes
Abstract
| < More recent | Older article > |
Abstract
A universal microchip was developed for genotyping Influenza A viruses. It contains two sets of oligonucleotide probes allowing viruses to be classified by the subtypes of hemagglutinin (H1-H13, H15, H16) and neuraminidase (N1-N9). Additional sets of probes are used to detect H1N1 sw...
|
PMID: 21559081
PDF is available here.
PDF is available here.
Abstract
We collected a total of 1000 urine samples, and 325 of these samples tested positive for a UTI via traditional culturing techniques; additionally, all 325 of these samples tested positive with the Eub338 probe in FISH analysis. FISH analyses with species-specific probes were performed in parallel to...
|
PMID: 20807557
PDF is available here.
PDF is available here.
Abstract
We developed the high-throughput Universal Probe Salmonella Serotyping (UPSS) technique based on nano liter PCR. In comparison to the classical approach, where O- and H-antisera are applied, the UPSS relies on specific gene content amplification of Salmonella spp. by a universal TaqMan assay for all...
|
PMID: 20869995
PDF is available here.
PDF is available here.
Abstract
We have adapted a clone-FISH approach known from prokaryotes to optimize the hybridization conditions of a designed oligonucleotidic probe specific to Chytridiales (i.e. the largest group of the true-fungal division of Chytridiomycota), before application to natural samples using the CARD-FISH appro...
|
PMID: 20849888
PDF is available here.
PDF is available here.
Abstract
A novel synthesis of 4-[(18)F]fluorobenzylamine ([(18)F]FBA) by means of transition metal-assisted sodium borohydride reduction of 4-[(18)F]fluorobenzonitrile ([(18)F]FBN) is described. This approach could successfully be extended to borohydride exchange resin (BER) enabling a viable option for use...
|
PMID: 20740244
PDF is available here.
PDF is available here.
Abstract
DNA piezoelectric biosensors have become a promising tool in molecular medicine since they do not require any label or staining. Here, a DNA piezoelectric biosensor based on a quartz crystal microbalance (QCM) was created to identify abnormal genes causing α-thalassemia 1 (SEA deletion).
The functi...
|
PMID: 20578968
PDF is available here.
PDF is available here.
Chemical Communications (46)31 2010
Abstract
Using atomic microscopy imaging, probe DNA sequence self-assemblies developed on Si(100) substrates undergo a conformational transition from an extended stem-loop structure to a double helix; such assemblies readily report on DNA molecular recognition events and should be suitable as a label-free, D...
|
PMID: 20596585
PDF is available here.
PDF is available here.
Abstract
We report that this hydrogen bonding pattern can be replaced by metal-ligation between specific metal ions and the natural bases. To demonstrate the feasibility of this principle, two linear oligonucleotides and silver ions were examined as models for DNA hybridization assay and adenosine triphospha...
|
PMID: 20597497
PDF is available here.
PDF is available here.
Abstract
A microbial diagnostic microarray for the detection of the most relevant bacterial food- and water-borne pathogens and indicator organisms was developed and thoroughly validated. The microarray platform based on sequence-specific end labelling of oligonucleotides and the pyhylogeneti...
|
PMID: 21255342
PDF is available here.
PDF is available here.
Abstract
A significantly increased frequency of HLA-B27 (corrected P = 0.01) and DQB1*0301 (corrected P = 0.0009) alleles and decreased frequency of HLA-DQB1*0302 (corrected P = 0.00001) were identified in patients compared to healthy controls. CONCLUSION: These data suggest that genes located within the MHC...
|
PMID: 20550778
PDF is available here.
PDF is available here.
Abstract
Antagomir-205 markedly reduced the level of microRNA-205 in NHCEKs and significantly enhanced adhesion ability of NHCEKs (P<0.01). Further protein analysis validated that inhibition of microRNA-205 increased the number of phosphorylated FAK and phosphorylated Pax, and decreased filamentous actin. CO...
|
PMID: 20598226
PDF is available here.
PDF is available here.
Abstract
Our results show that fish intermediate hosts play a role in the transmission of metastrongyloid nematodes of harbour porpoises and harbour seals.
(c) 2010 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved....
|
PMID: 20123100
PDF is available here.
PDF is available here.
Journal of Virological Methods (166)1-2 2010
Abstract
Newcastle disease virus (NDV), also designated avian paramyxovirus type 1 (APMV-1), is a serious pathogen of poultry, causing highly contagious Newcastle disease (ND), with high morbidity or mortality, depending on the strain. Accordingly, rapid and reliable detection of APMV-1 and differentiation b...
|
PMID: 20170680
PDF is available here.
PDF is available here.
Abstract
Contagious bovine pleuropneumonia (CBPP) is the most serious cattle disease in Africa, caused by Mycoplasma mycoides subsp. mycoides small-colony type (SC). CBPP control strategies currently rely on vaccination with a vaccine based on live attenuated strains of the organism. Recently, an lppQ(-) mut...
|
PMID: 20381545
PDF is available here.
PDF is available here.
Nucleic Acids Research (38)11 2010
Abstract
We developed a new PDF, pseudo probe binding energy (PPBE), by iteratively fitting dinucleotide positional weights and dinucleotide stacking energies until the average residue sum of squares for the model was minimized. PPBE showed a better correlation with probe sensitivity and a better specificity...
|
PMID: 20236987
PDF is available here.
PDF is available here.
Archives of Virology (155)6 2010
Abstract
A real-time reverse-transcription PCR (rRT-PCR) that targets a region of the polymerase (L) gene was developed to detect all known lineages of avian paramyxovirus type 1 (APMV-1), also known as Newcastle disease virus (NDV). A panel of 23 viruses representing the current known phylogenetic diversity...
|
PMID: 20428904
PDF is available here.
PDF is available here.
Abstract
Despite progress with triplex-forming oligonucleotides or helix-invading peptide nucleic acids (PNAs), there remains a need for probes facilitating sequence-unrestricted targeting of double stranded DNA (dsDNA) at physiologically relevant conditions. Invader LNA probes, i.e., DNA duplexes with "+1 i...
|
PMID: 20401378
PDF is available here.
PDF is available here.
Abstract
We reported an increased frequency of the minor G-allele of single-nucleotide polymorphism (SNP) rs569356 (the only variant identified so far in the promoter region of the delta-opioid receptor gene (OPRD1)) in subjects with opioid dependence. In this study, we examined the functional significance o...
|
PMID: 20300121
PDF is available here.
PDF is available here.
Abstract
We sequenced katG of 108 INH-resistant M. tuberculosis clinical isolates. Consequently, 9 novel KatG mutants with a single-amino-acid substitution were found. All of these mutants had significantly lower INH oxidase activities than the wild type, and each mutant showed various levels of activity. Is...
|
PMID: 20211896
PDF is available here.
PDF is available here.
Abstract
We present a new method to prepare inverse opaline photonic beads with good spherical shape and superior optical performance by simply introducing an interfacial tension system into a template replication method. When the scaffolds of these beads were composed of poly(ethylene glycol) diacrylate hyd...
|
PMID: 20359181
PDF is available here.
PDF is available here.
Abstract
We used molecular methods to identify the major bacterial consortia associated with advanced dentin caries. These consortia are dominated by bacteria from the families Lactobacillaceae, Streptococcaceae, Veillonellaceae (formerly Acidaminococcaceae), Eubacteriaceae, and Lachnospiraceae from the phyl...
|
PMID: 20200294
PDF is available here.
PDF is available here.
Abstract
We tested their HIV-1 RNA (95%) than when we tested their HIV-1 DNA (88%), whereas at 6 weeks after sdNVP therapy, the proportion was greater with DNA (85%) than with RNA (67%) and remained higher with DNA (33%) than with RNA (11%) at the initiation of antiretroviral treatment (median, 45 weeks afte...
|
PMID: 20181911
PDF is available here.
PDF is available here.
Abstract
A large number of human papillomavirus (HPV) types, distributed over five papillomavirus genera, are detectable in the skin. HPV types belonging to the alpha, gamma, and mu genera have been detected in cutaneous warts. A state-of-the-art HPV genotyping assay for these cutaneous wart-associated HPV t...
|
PMID: 20237103
PDF is available here.
PDF is available here.
Abstract
We describe the development of a rapid qualitative fluorescent real-time polymerase chain reaction assay designed for the detection of BRAF deletion using 2 specific molecular beacons. The assay is able to detect in a single tube the homozygous as well the heterozygous genotypes. The procedure combi...
|
PMID: 20186005
PDF is available here.
PDF is available here.
Bing du xue bao = Chinese journal of virology / [bian ji, Bing du xue bao bian ji wei yuan hui] (26)2 2010
Abstract
Abstract
We developed a common oligonucleotide reference standard (CORS) method to address this problem. A unique 50-mer reference oligonucleotide probe was selected to co-spot with gene probes for each array feature. The complementary sequence was synthesized and labeled for use as the reference target, whi...
|
PMID: 20038701
PDF is available here.
PDF is available here.
Abstract
We evaluated a combined Nocardia PCR-based reverse line blot (RLB) hybridization assay based on 16S and 16S-23S rRNA gene spacer region polymorphisms to identify 12 American Type Culture Collection and 123 clinical Nocardia isolates representing 14 species; results were compared with results from 16...
|
PMID: 19955277
PDF is available here.
PDF is available here.
Abstract
We show that a replacement of singly labeled oligonucleotide probes with 5'-, 3'-doubly labeled probes at least doubles FISH signal intensity without causing specificity problems. Furthermore, Cy3-doubly labeled probes strongly increase in situ accessibility of rRNA target sites and thus provide mor...
|
PMID: 19966029
PDF is available here.
PDF is available here.
Abstract
Abstract
To understand soil benzene monooxygenase gene diversity by clone library construction and microarray profiling.
A primer set was designed, and benzene monooxygenase gene diversity was characterized in two benzene-amended soils. The dominant sequence types in the clone...
|
PMID: 19912525
PDF is available here.
PDF is available here.
Abstract
The RAS-MAPK syndromes are a group of clinically and genetically related disorders caused by dysregulation of the RAS-MAPK pathway. A member of this group of disorders, Noonan syndrome (NS), is associated with several different genes within the RAS-MAPK pathway. To date, mutations in...
|
PMID: 20302979
PDF is available here.
PDF is available here.
Abstract
We developed multiplexed assays for rapid, simultaneous pathogen detection and characterization of ciprofloxacin and doxycycline resistance in Bacillus anthracis, Yersinia pestis, and Francisella tularensis. These assays are SNP-based and use Multiplexed Oligonucleotide Ligation-PCR (MOL-PCR). The M...
|
PMID: 20865530
PDF is available here.
PDF is available here.
Abstract
We developed a new assay system which supports very high degrees of multiplexing. We developed a new class of mismatch-tolerant "sloppy" molecular beacons, modified them to provide an extended hybridization range, and developed a multiprobe, multimelting temperature (T(m)) signature approach to bact...
|
PMID: 19923485
PDF is available here.
PDF is available here.
Abstract
The aim of this work is to describe the first example of aptameric molecular beacon (MB)-based probe for the detection of recombinant human erythropoietin (rHuEPO-alpha) in physiological buffer, using a novel 35 nt ssDNA aptamer (807-35 nt) originally isolated by Systematic Evolution of Ligands by E...
|
PMID: 19836583
PDF is available here.
PDF is available here.
Abstract
A two-probe tandem DNA hybridization assay including capture DNA(1), probe DNA(2), and target DNA(3) was prepared. The long-lived luminescent europium complex doped nanoparticles (NPs) were used as the biomarker. The complex included in the particle was Eu(TTA)(3)(5-NH(2)-phen)-IgG (ETN-IgG), the eu...
|
PMID: 19836584
PDF is available here.
PDF is available here.
Abstract
Freshwater magnetotactic cocci within Alphaproteobacteria are of ecological interest due to their ubiquitous distribution in aquatic environments as well as their potential roles in iron cycling and the bulk magnetism of sediment. To effectively investigate the diversity and distribution of these co...
|
PMID: 20112228
PDF is available here.
PDF is available here.
Abstract
We propose a multiobjective evolutionary optimization method for oligonucleotide probe design based on the multiobjective nature of the probe design problem. The proposed multiobjective evolutionary approach has several distinguished features, compared with previous methods. First, the evolutionary...
|
PMID: 19556203
PDF is available here.
PDF is available here.
Abstract
Purpose: To examine the role of radiation-induced DNA double-strand break (DSB) structural organization in DSB repair, and characterize the structural features of 125I-induced DSBs that may impact the repair process.
|
PMID: 15962759
PDF is available here.
PDF is available here.
Abstract
Analysis of PCR products of 16S rDNA of 680 isolates from Mediterranean Sea mesocosm experiments with taxon-specific 16S rDNA oligonucleotides revealed that 262 isolates belonged to the alpha subclass of the class Proteobacteria. Partial 16S rDNA sequence analysis of selected isolates and oligonucle...
|
PMID: 10319472
PDF is available here.
PDF is available here.
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics (26)4 2009
Abstract
The MLPA data indicated that the two children had possible microdeletions in the WS critical region. The deletions were confirmed and both were maternal origin by polymorphism analysis. CONCLUSION: MLPA is a quick and convenient method for detecting deletion or duplication mutations. It can provide...
|
PMID: 20017297
PDF is available here.
PDF is available here.
Analytical Biochemistry (391)1 2009
Abstract
We have developed a novel method for genotyping both single and multiple SNPs that uses an intercalating dye and a probe labeled with a single fluorophore to affect a fluorescence energy transfer. Melting curve analysis is then used to distinguish true alleles from mismatched alleles. We term the ne...
|
PMID: 19433053
PDF is available here.
PDF is available here.
Analytical Biochemistry (391)1 2009
Abstract
Central composite face-centered (CCF) design and response surface methodologies were used to investigate the effect of probe and target concentration and particle number in immobilization and hybridization on a microparticle-based DNA/DNA hybridization assay. The factors under study...
|
PMID: 19435595
PDF is available here.
PDF is available here.
Abstract
Intraocular or systemic steroids fail to prevent light-induced photoreceptor degeneration in the T4R RHO dog retina. Finding that systemic dexamethasone prevents AP-1 activation, yet does not prevent retinal light damage, further supports the hypothesis that AP-1 is not the critical player in the ce...
|
PMID: 19234347
PDF is available here.
PDF is available here.
Abstract
We designed, synthesized, and tested a novel molecular probe, in which the antisense oligodeoxynucleotide (ASODN) was labeled with superparamagnetic iron oxide (SPIO), and its efficiency was examined by in vitro MR imaging after SK-Br-3 mammary carcinoma cell lines (oncocytes) transfection. The SPIO...
|
PMID: 19337837
PDF is available here.
PDF is available here.
ChemBioChem (10)7 2009
Abstract
We have explored the general applicability of pyrene absorbance and especially fluorescence as a probe to monitor RNA hybridization. The results reveal that the backbone of the probes, the number of pyrene units attached and the nature of the tether can all substantially affect the absorbance and fl...
|
PMID: 19373795
PDF is available here.
PDF is available here.
Nucleic Acids Research (37)8 2009
Abstract
Gene expression analysis performed through comparative abundance of transcripts is facing a new challenge with the increasing need to compare samples of known cell number, such as early embryos or laser microbiopsies, where the RNA contents of identical cellular inputs can by nature be variable. Whe...
|
PMID: 19336411
PDF is available here.
PDF is available here.
Nucleic Acids Research (37)8 2009
Abstract
We resolve this loop problem. We introduce AptaPIC, Aptamer-facilitated Protein Isolation from Cells, a technology that integrates (i) the development of aptamers for a protein in cell lysate and (ii) the utilization of the developed aptamers for protein isolation from the cell lysate. Using MutS pr...
|
PMID: 19304751
PDF is available here.
PDF is available here.
Nucleic Acids Research (37)8 2009
Abstract
We report that 'labeling extension values (LEVs)', which are correlation coefficients between probe intensities and probe positions, are highly correlated with the gene expression levels (GEVs) on eukaryotic Affymetrix microarray data. By analyzing LEVs and GEVs in the publicly available 2414 cel fi...
|
PMID: 19295132
PDF is available here.
PDF is available here.
Abstract
Fluorescent in situ hybridization (FISH) using taxon-specific, rRNA-targeted oligonucleotide probes is one of the most powerful tools for the rapid identification of harmful microorganisms. However, eukaryotic algal cells do not always allow FISH probes to permeate over their cell walls. Members of...
|
PMID: 19396247
PDF is available here.
PDF is available here.
Abstract
Standardization on both Spoligotyping method and parameters in result analysis and the usage of the analysis software were studied. Through this method, 224 M. tuberculosis clinical strains were classified into 2 clusters including 129 Beijing family strains and 95 non-Beijing family strains. The pr...
|
PMID: 19731534
PDF is available here.
PDF is available here.
Abstract
Few reports on in situ mRNA detection in bacteria have been published, even though a major aim in environmental microbiology is to link function/activity to the identity of the organisms. This study reports a reliable approach for the in situ detection of nifH mRNA using fluorescence...
|
PMID: 19217232
PDF is available here.
PDF is available here.
Abstract
We describe a DNA microarray comprised of 282 sixty-mer oligonucleotide probes to study the epidemiology of Salmonella enterica subsp. enterica isolates at the genotypic level. The probes detect targets encoding genes associated with pathogenicity, antibiotic resistance, fimbriae, prophages, flagell...
|
PMID: 19521880
PDF is available here.
PDF is available here.
Abstract
We provide a comprehensive review of the ligase-mediated techniques for bio-molecular analysis....
|
PMID: 19621570
PDF is available here.
PDF is available here.
Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases (27)1 2009
[Inhibition of CpG oligodeoxynucleotide on the development of pre-erythrocytic stage of Plasmodium].
Abstract
The cloned Py BY265 18S rRNA gene showed 98% similarity to Py 17XNL. The quantitative analysis model consisted by 18S rRNA and GAPDH showed positive correlation between the level of liver Plasmodium load and the sporozoite inoculation dose to mice. The Plasmodium load in CpG ODN pre-treated mice was...
|
PMID: 19459490
PDF is available here.
PDF is available here.
Abstract
We developed an in situ synthesized 60-mer oligonucleotide microarray for gene expression monitoring of these orthologous genes, consisting of approximately 6600 Sc-type genes and 3200 Lg-type genes. A comparison of the transcriptional profile of orthologous genes (e.g. Sc-type and Lg-type genes) in...
|
PMID: 19243081
PDF is available here.
PDF is available here.
Mechanisms of loss of heterozygosity in neurofibromatosis type 1-associated plexiform neurofibromas.
Abstract
We performed LOH analysis on 43 plexiform neurofibromas from 31 NF1 patients, the largest study of its kind to date. A total of 13 (30%) plexiform neurofibromas exhibited LOH involving 17q markers. In three tumors, LOH was found to be confined to the NF1 gene region. However, in none of the tumors w...
|
PMID: 18800150
PDF is available here.
PDF is available here.
Bioconjugate Chemistry (20)1 2009
Abstract
We have presented a proof-of-concept for the detection of a target nucleic acid sequence based on the reassembly of the bioluminescent reporter Renilla luciferase (Rluc), which is driven by DNA hybridization. Although, reassembly of Rluc though protein interactions has been demonstrated by others, t...
|
PMID: 19063714
PDF is available here.
PDF is available here.
Analytica Chimica Acta (632)2 2009
Abstract
The impact of substrates for probe immobilization was studied using a microbial diagnostic microarray consisting of probes designed against the pmoA genes of methanotrophs and functionally related bacteria. The proprietary ARChip Epoxy was compared to seven epoxy-modified competitor slides, and one...
|
PMID: 19110100
PDF is available here.
PDF is available here.
Abstract
We propose here a new approach based on the principle that a probe-set which hybridizes to more than one transcript can be recognized because it produces a signal significantly different from others assigned to the particular gene, allowing it to be detected as an outlier in the group and eliminated...
|
PMID: 19436837
PDF is available here.
PDF is available here.