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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Oligonucleotide Probes (0):
Recent article
Oligonucleotide Probes
BMC Genomics (10)1 2009
Abstract
We have developed a robust microarray and PCR-based method, Transposon-Mediated Differential Hybridisation (TMDH), that uses novel bioinformatics to identify transposon inserts in genome-wide libraries. Following a microarray-based screen, genes lacking transposon inserts are re-tested using a PCR a...
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PMID: 19570206
PDF is available here.
PDF is available here.
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BMC Genomics (10)1 2009
Abstract
We designed 43,392 unique oligonucleotide probes representing 19,274 genes (93% of the estimated total gene number), and tested the hybridization performance of both DNA and RNA from different populations to determine the efficacy of probe design for transcriptome analysis using the Agilent array pl...
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PMID: 19744352
PDF is available here.
PDF is available here.
BMC Microbiology (9)1 2009
Abstract
Forty-two out of the ninety-eight suspected tissues examined had culture properties characteristic of mycobacteria from which 31 isolates yielded interpretable spoligotypes. This technique showed good discriminatory power (HGDI = 0.98), revealing 10 different spoligotype patterns. Twenty-seven isola...
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PMID: 19619309
PDF is available here.
PDF is available here.
Abstract
We present a detailed protocol for performing nanopore force spectroscopy on DNA duplexes using one or more alpha-hemolysin nanopores. We present the details of the measurement of the duplex survival probability under force, and show that dissociation time scales for duplexes that are perfectly comp...
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PMID: 19488698
PDF is available here.
PDF is available here.
Abstract
DNA replication studies often rely on analysis of replication intermediates, such as progressing replication forks and growing nascent strands. The assay presented here for replication at telomeres in the yeast Saccharomyces cerevisiae is based on the analysis of nascent DNA strands prior to the lig...
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PMID: 19563106
PDF is available here.
PDF is available here.
Experimental Parasitology (121)1 2009
Abstract
Cyathostome populations in horses on two farms located in central Italy with a history of fenbendazole (FBZ) resistance were investigated with the Faecal Egg Count Reduction Test to evaluate the susceptibility to oxibendazole and moxidectin. Faecal eggs were collected pre- and post-treatment on each...
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PMID: 18950625
PDF is available here.
PDF is available here.
Abstract
Shigella is the cause of shigellosis or bacillary dysentery, the occurrence of which is estimated to be 165 million cases per year worldwide, resulting in 1.1 million deaths. Rapid and reliable assays for detecting and identifying Shigella in food, environmental and clinical samples are therefore ne...
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PMID: 19074655
PDF is available here.
PDF is available here.
Abstract
To control the antibiotic resistance epidemic, it is necessary to understand the distribution of genetic material encoding antibiotic resistance in the environment and how anthropogenic inputs, such as wastewater, affect this distribution. Approximately two-thirds of antibiotics administered to huma...
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PMID: 18997031
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (134)1 2009
Abstract
Our specific probe design, hybridisation and signal transduction paths make the analytical process remarkably simple, relatively low cost and, thus, well suited for low throughput analysis of clinically relevant samples....
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PMID: 19082174
PDF is available here.
PDF is available here.
Nucleic Acids Research (37)Database issue 2009
Abstract
RTPrimerDB (http://www.rtprimerdb.org) is a freely accessible database and analysis tool for real-time quantitative PCR assays. RTPrimerDB includes records with user submitted assays that are linked to genome information from reference databases and quality controlled using an in silico assay evalua...
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PMID: 18948285
PDF is available here.
PDF is available here.
Nucleic Acids Research (37)Database issue 2009
Abstract
OligoArrayDb is a comprehensive database containing pangenomic oligonucleotide microarray probe sets designed for most of the sequenced genomes that are not covered by commercial catalog arrays. The availability of probe sequences, associated with custom microarray fabrication services offered by ma...
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PMID: 18948290
PDF is available here.
PDF is available here.
Abstract
The glass bead is a new biochip support material for immobilization biomolecules, due to its independence and convenient rearrangement. In order to optimize the immobilization efficiency of oligonucleotides onto glass beads and obtain the highest hybridization efficiency, three commonly used couplin...
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PMID: 18491234
PDF is available here.
PDF is available here.
Abstract
The real-time PCR assay is a valid alternative of the standard plate-based assay. SIGNIFICANCE AND IMPACT OF THE STUDY: The real-time PCR assay decreased the time between sample collection and result from 2 days to 2 h, while analysis cost did not increase. The gelatine-producing industry can ensure...
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PMID: 19018957
PDF is available here.
PDF is available here.
Abstract
A novel fluorescent oligonucleotide probe containing pyrene-labeled C8 alkylamino-substituted 2'-deoxyguanosine and a practically useful 3'- and 5'- ends-free self-quenched molecular beacons (MB) were designed. A unique MB detectable by pyrene excimer fluorescence was also demonstrat...
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PMID: 19749300
PDF is available here.
PDF is available here.
Abstract
We designed tandems of oligo(2'-O-methylribonucleotides) conjugates containing two bispyrene (5'-bisPyr and 3'-bisPyr) groups on their junction for detection of C677T polymorphism in the methylenetetrahydrofolate reductase gene (MHTFR). The potential of SNP detection with multipyrene tandems of olig...
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PMID: 19749301
PDF is available here.
PDF is available here.
Abstract
We describe a new ferrocene-based redox-reporter that electronically couples to the DNA Pi-electron system.
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PMID: 19749302
PDF is available here.
PDF is available here.
Abstract
We developed a new nucleic acid-based fluorescence probe for protein detection. The method is based on the scission of an aptamer into two probes, which are then attached with a chemically reactive fluorogenic compound. The protein-dependent association of the two probes accelerates a chemical react...
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PMID: 19749308
PDF is available here.
PDF is available here.
Abstract
We have developed a method for the detection of the endonuclease III reaction by fluorescence. The probes were 13-base-pair hairpin-shaped oligonucleotides containing one of the isomers of thymine glycol or 5,6-dihydrothymine as a damaged base at the center, and had a fluorophore and a quencher at t...
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PMID: 19749336
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)51 2008
Abstract
Several base-modified duplex-stabilizing deoxyribonucleoside 5′-triphosphates (dNTPs) have been evaluated as agents for enhancing the hybridization properties of primers and probes in real-time polymerase chain reaction (PCR). It was shown that pyrimidines substituted at the 5-position with bromine...
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PMID: 19046073
PDF is available here.
PDF is available here.
Orthodontic Waves (67)4 2008
Abstract
Journal of Periodontology (79)12 2008
Abstract
Smokers and never-smokers exhibited a similar and significant reduction in total bacteria counts over time. Irrespective of smoking status, deep sites consistently harbored greater quantities of total bacteria throughout the study. Higher numbers of the bacteria investigated were associated with ble...
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PMID: 19053920
PDF is available here.
PDF is available here.
Abstract
We report here a solution-based sandwich hybridization assay for the quantification of mRNAs from Escherichia coli without the need of prior RNA isolation. This assay makes use of four DNA oligonucleotide probes adjacently hybridizing to target RNA in clarified cell extracts. Two helper probes facil...
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PMID: 18952865
PDF is available here.
PDF is available here.
Biotechnology Letters (30)12 2008
Abstract
A novel method of one-step preparation of dual-labeled gold nanoparticle bio-probes was established by the electrostatic adsorption and the covalent bonding of gold nanoparticles with antibodies and thiol-modified oligonucleotides, respectively. Characterization of probes, the coverage and activity...
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PMID: 18670737
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)22 2008
Abstract
We screened several highly destabilizing analogs of G and C for one that could be used with 2-aminoadenine (nA) and 2-thiothymine (sT) to generate structure-free DNA that is fully accessible to complementary probes. The analogs, which included bioactive bases such as 6-thioguanine (sG), 5-nitrocytos...
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PMID: 18987000
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)22 2008
Abstract
We describe a method to identify and recover minor human immunodeficiency virus type 1 (HIV-1) sequence variants from a complex population. The original heteroduplex tracking assay (HTA) was modified by incorporating a biotin tag into the probe to allow for direct sequence determination of the query...
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PMID: 18948297
PDF is available here.
PDF is available here.