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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
RNA, Complementary (0):
Recent article
RNA, Complementary
Abstract
We describe the use of one method commonly used to generate transcriptomics data, namely the use of Affymetrix GeneChip microarrays. Data generated in transcriptomics experiments can be analysed using a multitude of approaches, but a common goal is to identify those transcripts whose abundance is al...
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PMID: 20972753
PDF is available here.
PDF is available here.
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Journal of Biological Chemistry (285)52 2010
Abstract
We obtained new results, which led us to propose a novel model for the initiation of viral RNA (vRNA) replication. In our study, we analyzed the initiation mechanisms of influenza virus vRNA and complementary RNA (cRNA) synthesis in vitro, using purified RNA polymerase (RdRp) and 84-nt model RNA tem...
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PMID: 20858902
PDF is available here.
PDF is available here.
Biology of Reproduction (83)1 2010
Abstract
We used a crossover design strategy to directly investigate the activity of murine and bovine PLCZ1 in both murine and bovine oocytes. In murine oocytes, injection of murine Plcz1 cRNA induced [Ca(2+)](i) oscillations at 10-fold lower concentrations than bovine PLCZ1, although in bovine oocytes bovi...
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PMID: 20357268
PDF is available here.
PDF is available here.
Abstract
We report here electrical measurements to examine how Na/K pump interactions with extracellular Na ions are influenced by C-terminal truncations. We deleted the last two (YY) or five (KESYY) residues in Xenopus laevis alpha1 Na/K pumps made ouabain resistant by either of two kinds of point mutations...
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PMID: 20548052
PDF is available here.
PDF is available here.
Abstract
We find that extensive complementarity between a target RNA and an Argonaute1-bound miRNA triggers miRNA tailing and 3'-to-5' trimming. In flies, Argonaute2-bound small RNAs--but not those bound to Argonaute1--bear a 2'-O-methyl group at their 3' ends. This modification blocks target-directed small...
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PMID: 20558712
PDF is available here.
PDF is available here.
Abstract
Methods in Molecular Biology (639)639 2010
Abstract
We applied the Arabidopsis Affymetrix tiling arrays to study the whole-genome transcriptome under drought, cold, and high-salinity stresses and identified a large number of drought, cold, and high-salinity stress-inducible genes and transcriptional units (TUs)....
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PMID: 20387044
PDF is available here.
PDF is available here.
Methods in Molecular Biology (632)632 2010
Abstract
We have pioneered the development of "ribonomic profiling" as a high-throughput method to study posttranscriptional regulation of gene expression in the cell. This method is also often referred to as RIP-CHIP. In this chapter, we describe how to use the RIP-CHIP technology to assess the posttranscri...
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PMID: 20217577
PDF is available here.
PDF is available here.
BMC Neuroscience (11) 2010
Abstract
We have investigated the contribution of the unusual NR3 N and N+1 site residues to the unique functional characteristics of receptors containing these subunits. Contrary to previous studies, we provide evidence that both the NR3 N and N+1 site amino acids are critically involved in mediating the un...
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PMID: 20958962
PDF is available here.
PDF is available here.
Biological Chemistry (391)2-3 2010
Abstract
We analyzed the conditions of dnRNA synthesis and determined their effect on the 6S RNA-mediated inhibition of RNA polymerase in vitro and in vivo. Upon nutritional upshift the RNA polymerase/6S RNA complex induces the rapid synthesis of dnRNAs, which form stable hybrids with the 6S RNA template. Th...
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PMID: 20030589
PDF is available here.
PDF is available here.
Biotechnology Letters (31)10 2009
Abstract
These results suggest that gene-specific silencing was induced by pRNA. This is the first time that such a route for gene silencing has been reported in a bacterium other than Escherichia coli. Gene-specific silencing induced by pRNA may be useful as a novel biotechnology tool for gene regulation in...
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PMID: 19543856
PDF is available here.
PDF is available here.
Abstract
We report here with the basis of microarray gene expression analysis that CXCR4 expressing glioma cells are capable of expressing PAI-1 mRNA and protein upon CXCL12 stimulation. Pretreatment with U0126, an inhibitor of mitogen activated protein kinase (MAPK)/extracellular signal-regulated kinase (ER...
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PMID: 19336886
PDF is available here.
PDF is available here.
Abstract
WML represent areas with a complex molecular phenotype. From this and previous evidence, WML may arise through tissue ischemia but may also reflect the contribution of additional factors like blood-brain barrier dysfunction. Differential expression of genes in WM[L] compared to WM[C] indicate a "fie...
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PMID: 19109541
PDF is available here.
PDF is available here.
Abstract
We report the identification of a residue G(1111) and two positively charged lysines immediately downstream of G(1111) in the intracellular linker connecting domains II and III of the cockroach sodium channel that are specifically involved in the action of type II pyrethroids, but not in the action...
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PMID: 19022275
PDF is available here.
PDF is available here.
Abstract
We also concluded that the toxic phloretin was not released from the conjugates. These results suggest that γ-PGA-phloridzin conjugates have potential as oral anti-diabetic drugs with high safety....
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PMID: 18977257
PDF is available here.
PDF is available here.
BMC Genomics (10)1 2009
Abstract
Immunohistochemical staining with an antibody to the N-terminus indicates a high level of myostatin propeptide present in the muscles of transgenic mice while there were no apparent differences in myostatin protein distribution in the muscle fibers between the transgenic and wild-type mice. Main ind...
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PMID: 19586544
PDF is available here.
PDF is available here.
Abstract
We have tagged PLCzeta with firefly luciferase. The expression of the luciferase protein tag in eggs is then measured by incubation in luciferin combined with luminescence imaging, or by the lysis of eggs in the presence of Mg-ATP and luciferin in a luminometer. The use of luciferase to monitor prot...
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PMID: 19085135
PDF is available here.
PDF is available here.
BioTechniques (45)5 2008
Abstract
We report a new stripping technique applicable to microarrays hybridized with cRNA with RNase H that is reproducible, leaving the DNA oligonucleotide probes intact and available for adding two additional uses. A Pearson correlation was used to assess the agreement between the first-round hybridizati...
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PMID: 19007341
PDF is available here.
PDF is available here.
Abstract
The duration of treatment and the concentration of BMP-2 affect the global expression pattern of MCF-7 cells. Among the regulated cancer-related genes, the cohort of the apoptosis-related genes showed the pronounced alterations. Our data point to a novel role of BMP-2 in the regulation of the PKR pa...
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PMID: 18446370
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (283)37 2008
Abstract
We recently identified a prostasin-dependent cleavage site (RKRK(186)) in the gamma subunit. We also demonstrated that the tract alpha D206-R231, between the two furin cleavage sites in the alpha subunit, as well as the tract gamma E144-K186, between the furin and prostasin cleavage sites in the gam...
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PMID: 18650438
PDF is available here.
PDF is available here.
Urological Research (36)3-4 2008
Abstract
We compared cAMP-stimulated 36Cl-, 14C-oxalate, and 35SO(4)2- efflux from Xenopus oocytes expressing hCFTR with water-injected control oocytes. hCFTR-expressing oocytes exhibited a large, reversible cAMP-dependent increase in whole cell conductance measured using a two-electrode voltage clamp and a...
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PMID: 18563405
PDF is available here.
PDF is available here.
Abstract
We identify two species of 5'-capped, approximately 18-25-nucleotide small RNAs. One was of antigenomic polarity, corresponding to the 5' end of hepatitis delta antigen (HDAg) mRNA, and interacted with HDAg and RNA polymerase II (Pol II), whereas the other mapped to a structurally analogous region o...
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PMID: 18552826
PDF is available here.
PDF is available here.
ChemBioChem (9)10 2008
Abstract
The stability and structure of nylon nucleic acid duplexes with complementary DNA and RNA strands was examined. Thermal denaturing studies of a series of oligonucleotides that contained nylon nucleic acids (1-5 amide linkages) revealed that the amide linkage significantly enhanced the binding affini...
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PMID: 18543259
PDF is available here.
PDF is available here.
Abstract
We characterized the function of this transporter by using the Xenopus laevis oocyte expression system. The transport activity of Lsi1 for silicic acid was significantly inhibited by HgCl2 but not by low temperature. Lsi1 also showed an efflux transport activity for silicic acid. The substrate speci...
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PMID: 18214526
PDF is available here.
PDF is available here.
Abstract
We report that PIP(2) activates BK channels independently of PIP(2) metabolites. PIP(2) enhances Ca(2+)-driven gating and alters both open and closed channel distributions without affecting voltage gating and unitary conductance. Recovery from activation was strongly dependent on PIP(2) acyl chain l...
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PMID: 18562499
PDF is available here.
PDF is available here.
Molecular Immunology (45)11 2008
Abstract
We analyzed in fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA) or osteoarthritis (OA), the expression profile of TLR-pathway related molecules, as well as the alterations induced by LPS stimulation in RA-FLS and the effect of VIP treatment. Cultured FLS were obtained...
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PMID: 18452992
PDF is available here.
PDF is available here.
Abstract
Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infection in infants, young children, and high-risk adults. Currently, there is no vaccine to prevent RSV infection, and the available therapeutic agents are of limited utility. Peptide-conjugated phosphorodiamidate morpho...
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PMID: 18443602
PDF is available here.
PDF is available here.
Abstract
Precise detection of target DNA and RNA sequences using chemically modified oligonucleotides is of crucial importance in gene analysis and gene silence. The hybridisation and base discrimination abilities of oligonucleotides containing 2'-O-methyl-2-thiouridine (s(2)Um) in homo- and hetero-duplexes...
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PMID: 18487052
PDF is available here.
PDF is available here.
Developmental Dynamics (237)6 2008
Abstract
We have identified two Semaphorin genes, Sema 3D and Sema 3F, as being specifically expressed in the developing parathyroid and thymus. The thymus and parathyroid originate from a common primordium that develops from the third pharyngeal pouch in mice. The expression of Sema 3D and Sema 3F was compa...
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PMID: 18489001
PDF is available here.
PDF is available here.
Abstract
We performed a functional analysis to study the role of 5'-3'UAR sequences using genomic and subgenomic dengue virus RNAs. We found that single mutations disrupting 5'-3' complementarity greatly compromised viral RNA synthesis. Although in most of the cases incorporation of compensatory mutations re...
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PMID: 18289628
PDF is available here.
PDF is available here.
Abstract
These plasticisers affected the steady-state mRNA levels of many human genes. Exposure to these compounds over many years has the potential to influence human health....
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PMID: 18473747
PDF is available here.
PDF is available here.
Abstract
We carried out all-atom molecular dynamics simulations that enabled us to cover the complete pathway of toxin approach and binding, and we validated our simulation results by using solid-state NMR data and electrophysiological measurements. Our structural model revealed a mechanism of cooperative to...
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PMID: 18462679
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (281)1 2008
Abstract
The gcvB gene encodes two small, nontranslated RNAs that regulate OppA and DppA, periplasmic binding proteins for the oligopeptide and dipeptide transport systems. Analysis of the gcvB sequence identified a region of complementarity near the ribosome-binding sites of dppA and oppA mRNAs. Several cha...
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PMID: 18312576
PDF is available here.
PDF is available here.
Abstract
We conclude that when optimum morphology is required for techniques such as laser microdissection, modified methacarn is the fixative least harmful to nucleic acids of the three tested in this study. In contrast, using traditional isolation techniques, RNA derived from tissue fixed in 10% NBF will n...
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PMID: 18291364
PDF is available here.
PDF is available here.
Abstract
We demonstrated that quercetin inhibits both glycine and 5-hydroxytryptamine type 3, (5-HT3A) receptor channel activities expressed in Xenopus oocytes. However, the effects of quercetin glycosides on glycine and 5-HT3A receptor channel activities are not well known. In the present study, we investig...
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PMID: 18379051
PDF is available here.
PDF is available here.
Abstract
The monocytes of a large proportion of bipolar patients and offspring of bipolar parents showed an inflammatory gene expression signature. This coherent set of genes opens new avenues for biomarker development with possibilities for disease prediction in individuals genetically at risk and for the s...
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PMID: 18391128
PDF is available here.
PDF is available here.
Abstract
We developed a mouse model to determine mechanisms by which DCAC and related acylating agents affect the liver. Autoimmune-prone female MRL +/+ mice were injected intraperitoneally with 0.2 mmol/kg of DCAC or dichloroacetic anhydride (DCAA) in corn oil twice weekly for six weeks. No overt liver path...
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PMID: 18293905
PDF is available here.
PDF is available here.
Abstract
Abstract
We called these 2058 genes spermatogenesis-related genes. By informatics analysis, the gene expression clustering, gene ontology, KEGG pathway and domain regions were predicted for describing the potential roles that may play during mouse spermatogenesis. This study provides a molecular basis for th...
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PMID: 18239273
PDF is available here.
PDF is available here.
Abstract
We expressed wild-type BK channels and mutants E386N/E389N, D326N, E329Q, and D326N/E329Q channels on Xenopus laevis oocytes, and measured the expressed currents under patch clamp. Contribution of E329 to the conductance is negligible and single channel conductance of D326N/E329Q channels measured a...
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PMID: 18227273
PDF is available here.
PDF is available here.
Journal of Molecular Biology (375)5 2008
Abstract
We analyzed the transcriptional response to persistent prion infection in a panel of three murine neural cell lines in vitro. Colony spot immunochemistry assays indicated that 65-100% of cells were infected in each line. Only the Nav1 gene was marginally modulated in one cell line, whereas transcrip...
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PMID: 18082765
PDF is available here.
PDF is available here.
Neuroscience (151)2 2008
Abstract
We hypothesized that the Slack channel may be affected by changes in intracellular concentrations of CO(2) and H(+). To examine this, we expressed the Slack channel in Xenopus oocytes and the Slo 2.2 protein was allowed to be inserted into the plasma membrane. Inside-out patch recordings were perfor...
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PMID: 18082331
PDF is available here.
PDF is available here.
Abstract
We identified 849 ncRNAs (of 1,328 examined) that are expressed in the adult mouse brain and found that the majority were associated with specific neuroanatomical regions, cell types, or subcellular compartments. Examination of their genomic context revealed that the ncRNAs were expressed from diver...
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PMID: 18184812
PDF is available here.
PDF is available here.
Abstract
I and II) were introduced. The bePOMs differ from the original POMs through the insertion of an additional methylene group into the backbone units, which increases the flexibility of the oligomers. bePOM I and II oligomers were synthesised using solid-phase peptide chemistry. Interestingly, UV therm...
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PMID: 18075653
PDF is available here.
PDF is available here.
Acta Biochimica Polonica (55)1 2008
Abstract
We postulate that their expression is regulated by redox state of plastoquinone (PQ) pool. Thus the PQ-mediated redox regulation of expression of Arabidopsis nuclear genes is probably limited to the irradiance window representing non-stressing conditions. We found that the promoter regions of the PQ...
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PMID: 18231654
PDF is available here.
PDF is available here.
Differential role of the influenza A virus polymerase PA subunit for vRNA and cRNA promoter binding.
Abstract
We demonstrate a differential requirement of the PA subunit for binding to the vRNA and cRNA promoters--specifically, PA is more important for binding to the cRNA than the vRNA promoter. Furthermore, five point mutations were identified in the L163-I178 region of PA, which resulted in an inhibition...
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PMID: 17905403
PDF is available here.
PDF is available here.
Abstract
Mouse and bovine PLCZ1 cRNAs were injected into matured bovine oocytes at different concentrations. Within the concentrations tested, mouse PLCZ1 injection activated bovine oocytes at a maximum rate when the pipette concentration of cRNA ranged from 0.25 to 1 mug/muL, while bovine PLCZ1 was optimal...
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PMID: 18284699
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
Here we report detection and validation of SFPs in cowpea using a readily available soybean (Glycine max) genome array. Robustified projection pursuit (RPP) was used for statistical analysis using RNA as a surrogate for DNA. Using a 15% outlying score cut-off, 1058 potential SFPs were enumerated bet...
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PMID: 18307807
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
We used the mRNA-tagging strategy to isolate transcripts specifically from C. elegans larval neurons. The WT-Ovation Pico System (WT-Pico) was used to amplify 2 ng of pan-neural RNA to produce labeled cDNA for microarray analysis. These WT-Pico-derived data were compared to microarray results obtain...
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PMID: 18284693
PDF is available here.
PDF is available here.
BMC Neuroscience (9)1 2008
Abstract
Using an assay system that allows AChR cluster assembly and disassembly to be studied separately and quantitatively, we describe a previously unrecognized role of the tyrosine phosphatase Shp2 in AChR cluster disassembly. Shp2 was robustly expressed in embryonic Xenopus muscle in vivo and in culture...
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PMID: 18647419
PDF is available here.
PDF is available here.
Abstract
Methods presently used to activate mare oocytes for assisted reproduction technologies provide low rates of advanced embryonic development. Because phospholipase Czeta (PLCzeta) is the postulated sperm-borne factor responsible for oocyte activation at fertilisation, the aim of the present study was...
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PMID: 19007551
PDF is available here.
PDF is available here.
Abstract
We describe a method to employ gene expression microarrays to study the transcriptional changes in redox-engineered cell lines that will overexpress MnSOD and/or catalase in the mitochondria....
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PMID: 19082960
PDF is available here.
PDF is available here.
Abstract
We investigated the transport properties of human SLC26A9 to determine its functional and pharmacological characteristics. SLC26A9 protein expression results in the appearance of an anionic current exhibiting an apparently linear current/voltage relationship and increases in (36)Cl influxes and effl...
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PMID: 18769029
PDF is available here.
PDF is available here.
Journal of Physiology (586)1 2008
Abstract
We show that the potency of a variety of endogenous and synthetic glycine-site coagonists varies between recombinant NMDARs such that the highest potency is seen at NR2D-containing and the lowest at NR2A-containing NMDARs. This heterogeneity is specified by the particular NR2 subunit within the NMDA...
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PMID: 17962328
PDF is available here.
PDF is available here.
Abstract
We defined the transcriptional changes in human epidermal keratinocytes 1, 4, 24, and 48 h after treatment with IL-1alpha. IL-1 significantly regulated 388 genes, including genes associated with proteolysis, adhesion, signal transduction, proliferation, and epidermal differentiation. IL-1 induces ma...
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PMID: 17941080
PDF is available here.
PDF is available here.
Abstract
The atmospheric epiphyte Tillandsia ionantha is capable of surviving drought stress for 6 months or more without any exogenous water supply via an as of yet to be determined mechanism. When plants were soaked in water for 3 h, leaves absorbed a remarkably large amount of water (30-40% on the basis o...
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PMID: 17674031
PDF is available here.
PDF is available here.
Developmental Biology (312)1 2007
Abstract
We examined the involvement of SFKs in the events leading to Ca2+ increase in rat eggs and demonstrated their involvement in RMII. Microinjecting mRNAs of active forms of Fyn or c-Yes but not of c-Src, into ovulated eggs, triggered RMII without evoking Ca2+ increase. A specific SFKs inhibitor (SU665...
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PMID: 17949706
PDF is available here.
PDF is available here.
Developmental Biology (312)1 2007
Abstract
We report that PLCzeta remains functional after proteolytic cleavage at the X-Y linker region. We found that N-terminal (33 and 37 kDa) and C-terminal fragments (27 kDa), presumably the result of PLCzeta cleavage at the X-Y linker region, were present in fresh sperm as well as in sperm extracts and...
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PMID: 18028898
PDF is available here.
PDF is available here.
Abstract
We hypothesize that in sporadic AD as well as PD a combination of environmental effects and gene expression may affect specific brain areas leading to neurodegeneration. We profiled gene expression of AD compared to PD and age matched controls post-mortem in the hippocampus, the gyrus-frontalis-medi...
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PMID: 18198416
PDF is available here.
PDF is available here.
Journal of Virology (81)24 2007
Abstract
We have cloned and characterized 17 novel miRNAs that originate from at least 12 precursor miRNAs in MCMV and are not homologous to HCMV miRNAs. In parallel, we applied a computational analysis, using a support vector machine approach, to identify potential precursor miRNAs in MCMV. Four of the top...
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PMID: 17928340
PDF is available here.
PDF is available here.