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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
RNA Probes (1):
Recent article
RNA Probes
Abstract
Quantitative multiplex real-time RT-PCR assays utilizing fluorescence resonance energy transfer (FRET) hybridization probes were developed for the detection of 13 respiratory viruses, including well recognized viral causes (respiratory syncytial virus, influenza viruses A and B, parainfluenza viruse...
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PMID: 20153377
PDF is available here.
PDF is available here.
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Methods in Molecular Biology (639)639 2010
Abstract
We have developed a simple but effective array platform for profiling plant miRNAs from various plant species. The array is composed of 188 non-redundant miRNA probes that can detect both conserved and species-specific miRNAs from most plant species, including Arabidopsis, rice, and poplar. In this...
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PMID: 20387051
PDF is available here.
PDF is available here.
Methods in Molecular Biology (629)629 2010
Abstract
We describe a protocol for miRNA in situ hybridization on mouse tissue cryosections using locked nucleic acid (LNA) probes. LNA probes demonstrate a much higher affinity to their complimentary RNAs compared to conventional RNA and DNA oligo probes, which allow detection of miRNAs in tissue sections...
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PMID: 20387156
PDF is available here.
PDF is available here.
Methods in Molecular Biology (629)629 2010
Abstract
MicroRNAs (miRNAs) represent a new class of small noncoding RNAs expressed in plants and animals. They are responsible for the regulation of up to 30% of human genes, thus underlying their influence on almost all cellular pathways. Recent studies have shown miRNAs to be differentially expressed in c...
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PMID: 20387154
PDF is available here.
PDF is available here.
Methods in Molecular Biology (630)630 2010
Abstract
We describe below two protocols using RT-LATE-PCR and provide specific guidelines for the general application of this technique. The first protocol is devised to quantify mRNA from mouse embryos by real-time PCR; the second protocol employs end-point analysis to detect a number of different viral-li...
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PMID: 20300997
PDF is available here.
PDF is available here.
Abstract
Accurately imaging endogenous or non-engineered RNA in live cells is not an easy task. Ideally, a probe and imaging strategy will have the following properties: (1) functional probes will be delivered to the desired cellular compartment, (2) they will achieve the correct level of affinity to bind ta...
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PMID: 20049827
PDF is available here.
PDF is available here.
Abstract
We explain techniques for use of RA reporter cells and RA reporter mice, and we describe in situ hybridization methods for the three major RA-degrading enzymes: CYP26A1, CYP26B1, and CYP26C1. Comparisons of the different indicators for sites of RA signaling demonstrate that local RA peaks and trough...
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PMID: 20552435
PDF is available here.
PDF is available here.
Methods in Molecular Biology (558)558 2009
Abstract
We describe a protocol for carrying out RNA FISH on male meiotic cells, together with detail on the important controls and common problems associated with this technique....
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PMID: 19685339
PDF is available here.
PDF is available here.
Abstract
Biotechnology Letters (31)2 2009
Abstract
We established two plasmid vectors in which the nucleotide sequence corresponding to the 3'-poly(A)+tail were included. By simply inserting full-length cDNAs lacking their 3'-poly(A)+tail into established plasmid vectors, full-length mRNAs having a 3'-poly(A)+tail were successfully prepared....
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PMID: 18810330
PDF is available here.
PDF is available here.
Analytical Biochemistry (385)1 2009
Abstract
Collection of bioanalytes from single cells is still a challenging technology despite the recent progress in many integrated microfluidic devices. A microfluidic dual capillary probe was prepared from a theta (theta)-shaped glass capillary to analyze messenger RNA (mRNA) from adherent cells and sphe...
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PMID: 19026981
PDF is available here.
PDF is available here.
Abstract
We found that, in the striatum, acute injections of the first generation antipsychotic (FGA) haloperidol or the second generation antipsychotic (SGA) olanzapine enhanced Arc mRNA levels, however such induction persisted for at least 2 h in haloperidol-treated rats whereas it waned as early as 1 h af...
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PMID: 18947986
PDF is available here.
PDF is available here.
Abstract
The great diversity of arthropod body plans, together with our detailed understanding of fruit fly development, makes arthropods a premier taxon for examining the evolutionary diversification of developmental patterns and hence the diversity of extant life. Crustaceans, in particular, show a remarka...
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PMID: 20147025
PDF is available here.
PDF is available here.
BMC Cell Biology (10) 2009
Abstract
After screening a library of newly synthesized stryrl dyes, we discovered three RNA binding dyes that provide morphological details of live parasites. Utilizing an inverted confocal imaging platform, live cell imaging of parasites increases parasite detection, improves the spatial and temporal resol...
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PMID: 19515257
PDF is available here.
PDF is available here.
Abstract
We describe the use of a northwestern and southwestern blot protocol for the evaluation of the DNA and RNA binding abilities of a novel putative methyl transferase HSPC133 (METTL5)....
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PMID: 19378081
PDF is available here.
PDF is available here.
Abstract
We showed the possibility that hnRNP C1/C2 enhanced SMN1 exon 7 splicing specifically....
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PMID: 19628962
PDF is available here.
PDF is available here.
Abstract
We reported that bispyrene-modified RNA probes (OMUpy2) were useful for RNA detection in homogeneous physiological media(1-2). The aim of this study is to establish in situ monitoring system to detect mRNAs in living cells by OMUpy2. We chose c-fos mRNA as a target RNA which is known as one of the i...
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PMID: 19749306
PDF is available here.
PDF is available here.
Abstract
We have recently isolated and characterized three zebrafish (Danio rerio) RNases, ZF-RNase-1,-2 and -3, endowed with diverse bioactivities, including microbicidal and angiogenic activities. In the present study we have analyzed their temporal and spatial gene expression profiles. Our results indicat...
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PMID: 18852033
PDF is available here.
PDF is available here.
Abstract
We present here the details of a general approach to fluorescent labeling of the CCA sequence with the fluorescent base analog pyrrolo-C (PyC) at position 75 as a molecular probe for monitoring the dynamics of the tRNA 3' end. Using Escherichia coli tRNA(Cys) as an example, we achieve such labeling...
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PMID: 18755841
PDF is available here.
PDF is available here.
Abstract
Fluorescence in situ hybridization (FISH) using telomeric and ribosomal sequences was performed in four species of toad genus Chaunus: C. ictericus, C. jimi, C. rubescens and C. schneideri. Analyses based on conventional, C-banding and Ag-NOR staining were also carried out. The four species present...
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PMID: 17965952
PDF is available here.
PDF is available here.
Acta Paediatrica (97)10 2008
Abstract
When applied to a material from 50 patients with positive blood culture and 245 patients with negative blood culture, the universal PCR showed a sensitivity of 42% (28-57), a specificity of 95% (92-97), a positive predictive value of 64% (45-80), and a negative predictive value of 89% (84-92) (95% c...
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PMID: 18624992
PDF is available here.
PDF is available here.
Abstract
We show that the expression of the angiogenesis-related, immediate early gene CCN1 (formerly known as CYR61), considered to be involved in tumor growth and invasiveness, is enhanced upon hypoxia stress primarily in a protein kinase A and cyclic AMP-responsive element binding protein (CREB) and CRE-d...
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PMID: 18819928
PDF is available here.
PDF is available here.
Abstract
These results demonstrated the phenotypic characteristics of IPHH and the feasibility in vitro culture system of HCV infected human samples. This system might be useful for study of pathogenesis of HCV infection or hepatocyte-based applications....
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PMID: 19077511
PDF is available here.
PDF is available here.
Abstract
We report an efficient protocol for whole-mount identification of the expression of mRNAs in the parthenogenetic pea aphid Acyrthosiphon pisum, an emerging model organism with a growing accumulation of genome sequencing data. In addition to steps common for most animal in situ hybridization protocol...
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PMID: 18481297
PDF is available here.
PDF is available here.
Abstract
I (group C) without liver metastasis were analysed. MAGE-A protein expression was evaluated by immunohistochemistry using a 6C1 monoclonal antibody and MAGE-A10 mRNA expression was detected by highly sensitive in situ hybridisation using a cRNA probe. Expressions of MAGE-A protein and MAGE-A10 mRNA...
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PMID: 18594524
PDF is available here.
PDF is available here.
Nature Genetics (40)6 2008
Abstract
We show that the second major step in the evolution of extreme fruit size was the result of a regulatory change of a YABBY-like transcription factor (fasciated) that controls carpel number during flower and/or fruit development....
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PMID: 18469814
PDF is available here.
PDF is available here.
Nature Genetics (40)6 2008
Abstract
We report that 33 multicopy gene families, representing approximately 273 mouse X-linked genes, are expressed in the testis and that this expression is predominantly in postmeiotic cells. RNA FISH and microarray analysis show that the maintenance of X chromosome postmeiotic repression is incomplete....
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PMID: 18454149
PDF is available here.
PDF is available here.
Abstract
We investigate the role of two auxiliary channel subunits, MEC-2 (stomatin-like) and MEC-6 (paraoxonase-like), by coexpressing them with constitutively active MEC-4/MEC-10 heteromeric channels in Xenopus oocytes. This work extends prior work demonstrating that MEC-2 and MEC-6 synergistically increas...
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PMID: 18504316
PDF is available here.
PDF is available here.
Molecular Pharmacology (73)5 2008
Abstract
We describe a novel cis-element in the 5' coding region of p53 mRNA and its interaction with heterogeneous nuclear ribonucleoprotein (hnRNP)C1/C2. This element is located in a putative hairpin loop structure, within the first 101 nucleotides downstream of the start codon. The binding of hnRNPC1/C2 i...
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PMID: 18296503
PDF is available here.
PDF is available here.
New England Journal of Medicine (358)18 2008
Abstract
A microRNA signature in molecularly defined, high-risk, cytogenetically normal AML is associated with the clinical outcome and with target genes encoding proteins involved in specific innate-immunity pathways.
Copyright 2008 Massachusetts Medical Society....
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PMID: 18450603
PDF is available here.
PDF is available here.
Abstract
These results provide molecular physiological evidence to support the roles of these two zCA isoforms in Na+ uptake and acid-base regulation mechanisms in zebrafish HR cells....
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PMID: 18322140
PDF is available here.
PDF is available here.
Abstract
Multiple enteric bacteria are present in PMP, and bacterial density and MUC2 expression is highest in the malignant form of PMP. Based on these observations, we propose that the bacteria observed in PMP may play a role in the mucinous ascites and perhaps promote carcinogenesis....
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PMID: 18299935
PDF is available here.
PDF is available here.
Nucleic Acids Research (36)6 2008
Abstract
LNA (locked nucleic acids, i.e. oligonucleotides with a methyl bridge between the 2' oxygen and 4' carbon of ribose) and 2,6-diaminopurine were incorporated into 2'-O-methyl RNA pentamer and hexamer probes to make a microarray that binds unpaired RNA approximately isoenergetically. That is, binding...
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PMID: 18252773
PDF is available here.
PDF is available here.
Abstract
A dual fluorescence reporter plasmid expressing EGFP and DsRed-Monomer from separate promoters was constructed for quantitative flow cytometry analysis. Cloning the hepatitis B virus (HBV) X gene into the 3' UTR region of DsRed-Monomer allowed quantifying the efficacy of ten siRNAs designed accordin...
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PMID: 18201553
PDF is available here.
PDF is available here.
Developmental Biology (315)2 2008
Abstract
We previously demonstrated that progenitors of both endothelium and smooth muscle cells in the aortic wall originated from the somite in the trunk of the embryo. However whether the contribution to vascular Smooth Muscle Cells (vSMC) is restricted to the aorta or encompasses other vessels of the tru...
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PMID: 18255054
PDF is available here.
PDF is available here.
Oral Diseases (14)2 2008
Abstract
The total bacterial count decreased in both groups, with a significant decrease in the test group. The counts and number of sites positive for P. gingivalis, T. forsythia and T. denticola significantly decreased in the test group after treatment, compared with those in the control group. Pocket dept...
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PMID: 18302679
PDF is available here.
PDF is available here.
Abstract
We studied Bufo arenarum CNBP (bCNBP) binding to single-stranded nucleic acid probes representing the main reported CNBP putative targets. We report that, although bCNBP is able to bind RNA and single-stranded DNA (ssDNA) probes in vitro, it binds RNA as a preformed dimer whereas both monomer and di...
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PMID: 17661353
PDF is available here.
PDF is available here.
Abstract
We have confirmed that the terminal purine loop is the major Gag binding site on SL3 and that a secondary Gag binding site exists at an internal purine bulge. We also demonstrate direct binding of oligonucleotide to their binding sites and confirm this interaction does not alter global RNA conformat...
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PMID: 18205073
PDF is available here.
PDF is available here.
Abstract
We examined a collection of 22 well-characterized DLBCL cell lines. The expression of miR-155 is heterogeneous in these cell lines and associates with NF-kappaB activity. We found that the expression of the primary miR-155 transcript reliably reflects that of the functional mature miR-155. Because m...
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PMID: 18262046
PDF is available here.
PDF is available here.
Biological Chemistry (389)1 2008
Abstract
The nightly bioluminescence of the dinoflagellate Gonyaulax is a circadian rhythm caused by the presence in cells of specialized bioluminescent organelles, termed scintillons, containing the reaction catalyst luciferase, the substrate luciferin and a luciferin-binding protein (LBP). LBP levels incre...
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PMID: 18095865
PDF is available here.
PDF is available here.
Abstract
We have shown that the telomerase RNA component hTR is highly expressed in the epithelium of non-dysplastic Oral Lichen Planus (OLP) lesions (11). We concluded that it is possible that this high expression might be related to the increased cellular proliferation seen in OLP rather than being an indi...
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PMID: 18712976
PDF is available here.
PDF is available here.
Abstract
We report on the detection of a novel group of dehalorespiring bacteria that reductively dechlorinate PCE to cis-dichloroethene by RNA-based stable isotope probing. Pristine river sediment was incubated at 15 degrees C with PCE at low aqueous concentration. Upon formation of dechlorination products,...
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PMID: 18211265
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
Using HRV and HEV as test cases, we assess a general design strategy for detecting and serotyping genetically diverse viruses. A minimal number of probe sequences (26 for HRV and 13 for HEV), which were potentially capable of detecting all serotypes of HRV and HEV, were determined and implemented on...
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PMID: 19046445
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
We deduced the sequences and genomic organisation of the different murine PR72/B" isoforms: three genes encode nine isoforms, five of which are abundantly expressed and give rise to genuine PP2A subunits. Thereby, one novel subunit was identified. Using Northern blotting, we examined the tissue-spec...
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PMID: 18715506
PDF is available here.
PDF is available here.
Abstract
Abstract
We examined the available genome sequences of Apis mellifera, Nasonia vitripennis, Tribolium castaneum, Anopheles gambiae and identified Sox family members which were classified by phylogenetics using the HMG domains. Using in situ hybridisation we determined the expression patterns of eight honeybe...
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PMID: 18439299
PDF is available here.
PDF is available here.
Abstract
These results suggested the high hybridization specificity of the small LNA-RNA probes to target small RNAs. Finally, the authors applied (33)P-LNA probes to detect miRNA let-7C expression in human cancer tissues. Let-7C was clearly present in lung, prostate, and colon cancers but undetectable in ov...
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PMID: 18188765
PDF is available here.
PDF is available here.
Abstract
Titanium is a highly biocompatible material and very osteogenic in vivo. However, how titanium regulates osteoblast activity to promote bone formation is incompletely characterized. We, therefore, attempted to get more information by using microRNA (miRNA) microarray techniques to investigate transl...
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PMID: 18780563
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
We designed CataCleave probes to detect SNPs located in the insulin-like growth factor 2 (IGF-2) gene and at position 702 within the NOD2/CARD15 gene. Probes were also designed to detect a six-basepair deletion in the amelogenin gene and a partially methylated target DNA. Discrimination between wild...
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PMID: 18484652
PDF is available here.
PDF is available here.
Abstract
A covalently modified heteroconjugate between linezolid and neomycin B leads to an enhanced and more specific binding affinity to hairpin RNA targets in comparison to neomycin B itself. This heteroconjugate was used as a lure to select linezolid-specific hairpin RNA from an Escherichia coli genome R...
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PMID: 17869523
PDF is available here.
PDF is available here.
Abstract
Enzymatic synthesis methods for the fluorinated 5'-triphosphate analogues 5F-UTP and 5F-CTP have been developed to facilitate 19F-labeling of RNAs for biophysical studies. HIV-2 TAR RNAs were synthesized using these analogues by in vitro transcription reactions using T7 RNA polymerase. The uniform i...
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PMID: 17990877
PDF is available here.
PDF is available here.
Abstract
1H NMR spectroscopy and molecular modelling have been used to investigate the binding of the DeltaDelta-and LambdaLambda-enantiomers of the dinuclear ruthenium(II) complex [[Ru(Me2bpy)2]2(mu-bpm)]4+ [Me2bpy = 4,4'-dimethyl-2,2'-bipyridine; bpm = 2,2'-bipyrimidine] to an RNA tridecanucleotide duplex...
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PMID: 18271485
PDF is available here.
PDF is available here.
Abstract
We have optimized detection channel depth and width for a microfluidic device, and investigated polydimethyl siloxiane components and observation setup to minimize background noise. Adopting optimized channel dimensions and setup for the probe, the fluorescent intensity increased 3.3-fold at the low...
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PMID: 17588151
PDF is available here.
PDF is available here.
Abstract
The tissue expressions of nine immune related genes in apparently healthy Pacific white shrimp Litopenaeus vannamei were analyzed by conventional RT-PCR, quantitative real time PCR (qPCR) and in situ hybridisation. The nine genes were beta-glucan binding protein-high density lipoprotein (BGBP-HDL),...
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PMID: 17964809
PDF is available here.
PDF is available here.
Molecular and Cellular Biochemistry (306)1-2 2007
Abstract
We provide direct evidence that the differences in cardiac hypertrophy between genders in ANP +/- mice is attributed to estrogen. Furthermore, estrogen may play a key role in slowing down the progression of salt-induced left ventricular hypertrophy in ANP +/- mice, in part, independent of the classi...
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PMID: 17713841
PDF is available here.
PDF is available here.
Bioinformatics (23)22 2007
Abstract
The application of machine-learning techniques to the features we have used is a useful and general approach for microRNA target gene prediction. Our technique produces fewer false positive predictions and fewer target candidates to be tested. It exhibits higher sensitivity and specificity than algo...
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PMID: 17925304
PDF is available here.
PDF is available here.
Bioinformatics (23)21 2007
Abstract
Our RNA-As-Graph-Pools (RagPools) web server offers a theoretical companion tool for RNA in vitro selection and related problems. Specifically, it suggests how to construct RNA sequence/structure pools with user-specified properties and assists in analyzing resulting distributions. This utility foll...
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PMID: 17855416
PDF is available here.
PDF is available here.
Analytical Biochemistry (369)2 2007
Abstract