Abstract
We employed a mouse model (Foxo3) in which follicles are assembled normally but then undergo synchronous activation. We developed a microarray-based approach for the systematic discovery of tissue-specific genes and, by applying it to Foxo3 ovaries and other samples, defined a surprisingly large set...
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PMID: 17660561
PDF is available here.
Abstract
We describe the most comprehensive study to date on gene expression during mouse inner ear (IE) organogenesis. Samples were microdissected from mouse embryos at E9-E15 in half-day intervals, a period that spans all of IE organogenesis. These included separate dissections of all discernible IE substr...
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PMID: 17660535
PDF is available here.
Abstract
We examined systemic and cellular ferritin regulation and trafficking in the model organism Drosophila melanogaster. We showed that ferritin H and L transcripts are coexpressed during embryogenesis and that both subunits are essential for embryonic development. Ferritin overexpression impaired the s...
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PMID: 17603097
PDF is available here.
Abstract
We hypothesize that prokineticin receptor-1 (PKR1/GPR73) signaling may contribute to cardiomyocyte survival or repair in myocardial infarction. Since we showed that prokineticin-2 and PKR1 are expressed in adult mouse heart and cardiac cells, we investigated the role of prokineticin-2 on capillary e...
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PMID: 17442730
PDF is available here.
Abstract
We delivered three MR probe variants with superparamagnetic iron oxide nanoparticles (SPION, a T2 susceptibility agent) linked to a phosphorothioate-modified oligodeoxynucleotide (sODN) complementary to c-fos mRNA (SPION-cfos) or beta-actin mRNA (SPION-beta-actin) and to sODN with random sequence (S...
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PMID: 17478745
PDF is available here.
Abstract
We investigated the IgG1 heavy chain (IGHG1) constant region gene and IgG protein expression in 6 cell lines, including epithelial cancer cells, and in tissues from 66 hyperplasias, adenomas, and carcinomas. We also studied the mechanism of IgG production in these cells by examining the expression o...
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PMID: 17475920
PDF is available here.
Abstract
We investigated IGF-I and IGF binding proteins (IGFBPs) in an adult (17 months old) transgenic (GH-overexpressing) tilapia (Oreochromis niloticus). The transgenics showed an around 1.5-fold increase in length and an approximately 2.3-fold higher weight than the non-transgenics. Using radioimmunoassa...
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PMID: 17431805
PDF is available here.
Abstract
We study the problem of selecting control clones in DNA array hybridization experiments. The problem arises in the OFRG method for analyzing microbial communities. The OFRG method performs classification of rRNA gene clones using binary fingerprints created from a series of hybridization experiments...
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PMID: 17787064
PDF is available here.
Abstract
RNA from circulating blood reticulocytes was utilized to provide a robust description of genes transcribed at the final stages of erythroblast maturation. After depletion of leukocytes and platelets, Affymetrix HG-U133 arrays were hybridized with probe generated from the reticulocyte total RNA (bloo...
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PMID: 17405831
PDF is available here.
Abstract
We employed a miRNA microarray to detect 892 miRNAs in order to evaluate the expression patterns of miRNA. The expression of 19 miRNAs was significantly different between immature and mature individuals. Fourteen miRNAs were significantly upregulated and five miRNAs were downregulated in immature mi...
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PMID: 17641090
PDF is available here.
Naoto Kubota,
Wataru Yano,
Tetsuya Kubota,
Toshimasa Yamauchi,
Shinsuke Itoh,
Hiroki Kumagai,
Hideki Kozono,
Iseki Takamoto,
Shiki Okamoto,
Tetsuya Shiuchi,
Ryo Suzuki,
Hidemi Satoh,
Atsushi Tsuchida,
Masao Moroi,
Kaoru Sugi,
Tetsuo Noda,
Hiroyuki Ebinuma,
Yoichi Ueta,
Tatsuya Kondo,
Eiichi Araki,
Osamu Ezaki,
Ryozo Nagai,
Kazuyuki Tobe,
Yasuo Terauchi,
Kohjiro Ueki,
Yasuhiko Minokoshi and
Takashi Kadowaki
Abstract
We show that adiponectin enhances AMPK activity in the arcuate hypothalamus (ARH) via its receptor AdipoR1 to stimulate food intake; this stimulation of food intake by adiponectin was attenuated by dominant-negative AMPK expression in the ARH. Moreover, adiponectin also decreased energy expenditure....
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PMID: 17618856
PDF is available here.
Abstract
We compared alterations in food intake, body weight, fat mass, plasma glucose, and leptin levels, and patterns of hypothalamic gene expression in OLETF rats and mice lacking CCK1 receptors in response to a 10-wk exposure to HFD. Compared with Long-Evans Tokushima Otsuka (LETO) control rats, OLETF ra...
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PMID: 17409266
PDF is available here.
Abstract
These results suggest that the expression of EBV may be involved in the pathogenesis of RCC and nephroblastoma....
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PMID: 17549343
PDF is available here.
Abstract
We describe a new way to generate molecular probes for specific recognition of cancer cells. Molecular medicine will require a large number of probes for molecular recognition and characterization of a variety of diseased cells. Aptamers, single-stranded DNA/RNA probes, are poised to become a chemis...
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PMID: 17530817
PDF is available here.
Abstract
We asked whether somatodendritic levels of human BC200 RNA are deregulated in AD brains. We found that in normal aging, BC200 levels in cortical areas were reduced by >60% between the ages of 49 and 86. In contrast, BC200 RNA was significantly up-regulated in AD brains, in comparison with age-matche...
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PMID: 17553964
PDF is available here.
Abstract
We sought to investigate and characterize the RNA pool found in bovine spermatozoa. The main goals of this study were to examine RNA integrity and survey the mRNA found in spermatids and spermatozoa. Assessment of mRNAs integrity was performed by three approaches: microelectrophoresis, comparative s...
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PMID: 17636162
PDF is available here.
Abstract
Aptamers selected against cultured neoplastic cells can effectively be used as molecular probes for recognition of neoplastic cells in patient samples. Cell-based aptamer selection can be used to generate aptamer probes to obtain molecular signatures of neoplastic cells in patient samples....
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PMID: 17463173
PDF is available here.
Abstract
We provide evidence that Kirrel, a homolog of receptor proteins that organize myoblast fusion in Drosophila melanogaster, is necessary for muscle precursor fusion in zebrafish. Within developing somites, Kirrel expression localized to membranes of fusion-competent myoblasts of the fast-twitch lineag...
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PMID: 17529975
PDF is available here.
Abstract
This study demonstrated the validity of the SQ method to easily quantify the concentration and to determine the growth rate of specific micro-organisms present in mixed cultures. SIGNIFICANCE AND IMPACT OF THE STUDY: The proposed method can be directly utilized for quantification and kinetic charact...
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PMID: 17576227
PDF is available here.
Abstract
We examine EcR requirements in the FC at late stages of oogenesis. EcR protein is ubiquitous in the FC but its activity is restricted, visualized by activity of the "ligand sensor" hs-GAL4-EcR ligand binding domain fusion and EcRE-lacZ reporter gene expression. GAL4-EcR is activated in the FC by an...
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PMID: 17436275
PDF is available here.
Abstract
We tested a urethral swab from 550 HIV-negative males with genital warts and 64 HIV-negative males clinically free of genital warts, partners of HPV-infected females, using the HC2 Microplate assay. A perianal swab was also obtained from patients with perianal warts. In the first group, HPV DNA of a...
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PMID: 17524194
PDF is available here.
Abstract
We describe Plxdc2 expression in the embryonic mouse, with particular emphasis on the developing central nervous system. Using light microscopy and optical projection tomography (OPT), we analyse RNA in situ hybridization patterns and expression of two reporter genes, beta-geo (a fusion of beta-gala...
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PMID: 17280871
PDF is available here.