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RNA, Algal (0):
Recent article
RNA, Algal
Abstract
Despite the significance of diatoms in biomonitoring, many aspects of their biodiversity and geographical distribution are poorly understood. Recent evidence from molecular data has shown that traditional cosmopolitan and euryvalent morphospecies are often heterogeneous, containing cryptic or pseudo...
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PMID: 20097131
PDF is available here.
PDF is available here.
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Abstract
We hypothesized that the CMG021C gene product functions as an NiR in C. merolae. To test this hypothesis, we developed a method for targeted gene disruption in C. merolae. In support of our hypothesis, we found that a CMG021G null mutant in comparison with the parental strain showed decreased cell g...
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PMID: 20375110
PDF is available here.
PDF is available here.
Abstract
We show that the expression of the rb38, rb47 and rb60 genes, as well as the nuclear-encoded psbO gene that directs the synthesis of OEE1 (oxygen evolving enhancer 1), is differentially regulated by red light. Further elucidation of the signal transduction pathway shows that calmodulin is an importa...
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PMID: 20061301
PDF is available here.
PDF is available here.
Molecular Biotechnology (43)2 2009
Abstract
The rapid and effective method for the isolation of RNA from green microalga Ankistrodesmus convolutus based on homogenization in a simple CTAB buffer and selective precipitation of RNA with lithium chloride is developed. This procedure avoids the use of toxic chaotropic agents and phenol while high...
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PMID: 19507070
PDF is available here.
PDF is available here.
Biotechnology Letters (31)9 2009
Abstract
A marine microalga, strain JPCC GA0024 was selected as high amount of neutral lipid producers from marine microalgal culture collection toward biofuel production. The strain was tentatively identified as Scenedesmus rubescens by 18S rDNA analysis. The growth of strain JPCC GA0024 was influenced by a...
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PMID: 19475337
PDF is available here.
PDF is available here.
Abstract
Within the Chlorophyta, EF-1alpha is shown to be present in three ulvophycean orders (i.e., Dasycladales, Bryopsidales, Siphonocladales) and the genus Ignatius. Models describing gene gain-loss dynamics revealed that the presence of EF-1alpha, EFL or both genes along the backbone of the green plant...
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PMID: 19216746
PDF is available here.
PDF is available here.
Journal of Virology (82)24 2008
Abstract
Paramecium bursaria chlorella virus 1 (PBCV-1) is the prototype of a family of large, double-stranded DNA, plaque-forming viruses that infect certain eukaryotic chlorella-like green algae from the genus Chlorovirus. PBCV-1 infection results in rapid host membrane depolarization and potassium ion rel...
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PMID: 18842725
PDF is available here.
PDF is available here.
Journal of Cell Biology (182)4 2008
Abstract
We show that specific stress stimuli induce the assembly of RNA granules in an organelle with bacterial ancestry, the chloroplast of Chlamydomonas reinhardtii. These chloroplast stress granules (cpSGs) form during oxidative stress and disassemble during recovery from stress. Like mammalian stress gr...
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PMID: 18710928
PDF is available here.
PDF is available here.
Abstract
We should not have been able to observe that the most frequent class of permuted genes is that of the anticodon loop type, for the simple reason that this class would derive by random permutation from a class of non-permuted tRNA genes, which instead is the rarest. This would not explain the high fr...
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PMID: 18513750
PDF is available here.
PDF is available here.
Abstract
Haematococcus pluvialis, a green alga, accumulates carotenoids, predominantly astaxanthin, when exposed to stress conditions. In the present work, changes in the pigment profile and expression of carotenogenic genes under various nutrient stress conditions and their regulation were studied. Nutrient...
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PMID: 18343887
PDF is available here.
PDF is available here.
Abstract
We analyzed expressed sequence tags from Euglena to further characterize the structural features of the LHC polyprotein-coding genes and to examine the evolution of this multigene family. Of the 19 different LHC transcriptional units we detected, 17 encoded polyproteins composed of both tandem and n...
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PMID: 17947344
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
Like its three chlorophycean homologues, the 196,547-bp Oedogonium chloroplast genome displays a distinctive architecture. This genome is one of the most compact among photosynthetic chlorophytes. It has an atypical quadripartite structure, is intron-rich (17 group I and 4 group II introns), and dis...
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PMID: 18558012
PDF is available here.
PDF is available here.
Abstract
Abstract
A unicellular microalga, Chlamydomonas reinhardtii, was isolated from rice paddy-field soil and water samples and used in the biotransformation of hydrocortisone (1). This strain has not been previously tested for steroid bioconversion. Fermentation was carried out in BG-11 medium supplemented with...
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PMID: 18830164
PDF is available here.
PDF is available here.
BMC Genomics (9)1 2008
Abstract
The H. andersenii mtDNA is 60,553 bp in size and encodes 30 structural RNAs and 36 protein-coding genes, all located on the same strand. A prominent feature of the genome is the presence of a approximately 20 Kbp long intergenic region comprised of numerous tandem and dispersed repeat units of betwe...
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PMID: 18474103
PDF is available here.
PDF is available here.
Plant Cell Online (19)11 2007
Abstract
We report the identification of the RBP40 gene based on mass spectrometric analysis of its purified product. Unexpectedly, this was found to be identical to the previously described RNA binding protein RB38, which had been suggested to be involved in the regulation of D1 protein synthesis. However,...
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PMID: 18055611
PDF is available here.
PDF is available here.
Plant Cell Online (19)11 2007
Abstract
Our data indicate that NIT2 is a central regulatory gene required for nitrate signaling on the Chlamydomonas NIA1 gene promoter and that intracellular nitrate is needed for NIT2 function and to modulate NIA1 transcript levels....
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PMID: 18024571
PDF is available here.
PDF is available here.
Abstract
We verified that these genes are expressed and produce mature tRNAs that are aminoacylated. Analysis of tRNA-processing intermediates for these genes indicates an unusual processing pathway in which the termini of the tRNA precursor are ligated, resulting in formation of a characteristic circular RN...
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PMID: 17947580
PDF is available here.
PDF is available here.
Abstract
A large-scale screen for genes induced during sexual development was performed in the heterothallic oomycete Phytophthora infestans, the potato blight agent. Of 15,644 unigenes on an Affymetrix chip, 87 were induced >10-fold during mating, with 28 induced >100-fold. This was validated in independent...
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PMID: 17215149
PDF is available here.
PDF is available here.
Abstract
We show that the unicellular alga Chlamydomonas reinhardtii contains miRNAs, putative evolutionary precursors of miRNAs and species of siRNAs resembling those in higher plants. The common features of miRNAs and siRNAs in an alga and in higher plants indicate that complex RNA-silencing systems evolve...
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PMID: 17538623
PDF is available here.
PDF is available here.
Abstract
Abstract
Evolutionary studies in lichen associations follow that of the fungal symbiont (mycobiont), which is the symbiont after which the lichen is named and forms the majority of the thallus. However, evolution of the algal partner (photobiont) is important to maintain compatibility between symbionts and t...
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PMID: 17460809
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (268)2 2007
Abstract
Saprolegnia bulbosa sp. nov. was isolated from floating and decaying twigs and leaves in El Gato stream, Partido de La Plata, Buenos Aires Province, Argentina. The distinctive characteristics of S. bulbosa are the product of smooth oogonia and predominantly contorted monoclinous, androgynous and dic...
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PMID: 17328749
PDF is available here.
PDF is available here.
Microbiology (153)Pt 3 2007
Abstract
Phytophthora infestans is the cause of late blight, a devastating and re-emerging disease of potato. Significant advances have been made in understanding the biology of P. infestans, and in the development of molecular tools to study this oomycete. Nevertheless, little is known about the molecular b...
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PMID: 17322195
PDF is available here.
PDF is available here.
Abstract
The order Trentepohliales is a widespread group of terrestrial green algae. As currently circumscribed, it includes six genera (Cephaleuros, Phycopeltis, Physolinum, Printzina, Stomatochroon and Trentepohlia), the phylogenetic affinities of which are poorly understood. Sequences for the small subuni...
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PMID: 16825655
PDF is available here.
PDF is available here.
Abstract
This article brings forth recommendations from a workshop sponsored by the U.S. Environmental Protection Agency's Science to Achieve Results (STAR) and Environmental Monitoring and Assessment (EMAP) Programs and by the Council of State Governments, held during May 2002 in Kansas City, Kansas. The wo...
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PMID: 16779607
PDF is available here.
PDF is available here.
Current Microbiology (52)2 2006
Abstract
Pythium rhizo-oryzae sp. nov. was isolated from some soil samples taken in paddy fields in northeastern India. For quite a long time it was wrongly identified as Pythium catenulatum Mathews, because of the presence of numerous catenulate hyphal bodies. The sequencing of the ITS region of its nuclear...
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PMID: 16467992
PDF is available here.
PDF is available here.
BioTechniques (40)2 2006
Abstract
A new approach for simultaneously amplifying the 5' and 3' ends of a desired cDNA is described. The method combines the template-switching effect with inverse PCR, which generates the flanking 5' and 3' regions of a certain cDNA with very low or no background. It requires only minimal amounts of tot...
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PMID: 16526408
PDF is available here.
PDF is available here.
Abstract
We describe the solution NMR structure, electrostatic calculations, and detailed magnesium ion-binding surface of D5 RNA from the Pylaiella littoralis large ribosomal RNA intron (D5-PL). The overall structure consists of a hairpin capped by a GNRA tetraloop. The stem is divided into lower and upper...
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PMID: 16428604
PDF is available here.
PDF is available here.
Nucleic Acids Research (34)1 2006
Abstract
We report on the identification and characterization of three suppressor point mutations which overcome a translational defect caused by the deletion of a U-rich element in the 5'-untranslated region (5'-UTR) of the psbD mRNA in the green alga Chlamydomonas reinhardtii. All three suppressors affect...
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PMID: 16410618
PDF is available here.
PDF is available here.
Nucleic Acids Research (34)18 2006
Abstract
We have used the yeast three-hybrid system to identify chloroplast group II intron RNA-binding proteins, capable of interacting with the tscA RNA. Of 14 candidate cDNAs, 13 encode identical polypeptides with significant homology to members of the nuclear nucleosome assembly protein (NAP) family. The...
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PMID: 17012281
PDF is available here.
PDF is available here.
Plant Physiology (139)4 2005
Abstract
We report that in the green alga Chlamydomonas reinhardtii, PPO and FeC are each encoded by a single gene. Phylogenetic analysis indicates that C. reinhardtii PPO and FeC are most closely related to plant counterparts that are located only in chloroplasts. Immunoblotting results suggest that C. rein...
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PMID: 16306143
PDF is available here.
PDF is available here.
Plant Physiology (139)2 2005
Abstract
These results suggest that air-level [CO(2)] is required to trigger the transcription of ptca1 and that light affects the extent of acclimation. During acclimation to air for 120 h in the light, levels of ptca1 mRNA exhibited a periodic oscillation with a cycle of about 24 h, which, however, was not...
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PMID: 16169965
PDF is available here.
PDF is available here.
Plant Physiology (139)2 2005
Abstract
The first step in sexual differentiation of the unicellular green alga Chlamydomonas reinhardtii is the formation of gametes. Three genes, GAS28, GAS30, and GAS31, encoding Hyp-rich glycoproteins that presumably are cell wall constituents, are expressed in the late phase of gametogenesis. These gene...
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PMID: 16183845
PDF is available here.
PDF is available here.
Plant Molecular Biology (59)3 2005
Abstract
We constructed a red algal plastid DNA microarray of Cyanidioschyzon merolae covering almost all protein coding genes, and found that plastid genes are differentially activated by illumination. Run-on transcription assays using isolated plastids confirmed that activation takes place at the transcrip...
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PMID: 16235106
PDF is available here.
PDF is available here.
Abstract
We report a large-scale investigation of gene expression from compacted eukaryotic genomes. We have conducted EST surveys of the microsporidian Antonospora locustae and nucleomorphs of the cryptomonad Guillardia theta and the chlorarachniophyte Bigelowiella natans. In all three systems we find a hig...
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PMID: 16037215
PDF is available here.
PDF is available here.
Abstract
We verify the processing of all predicted intron-harboring tRNAs and demonstrate the splicing of the smallest introns (3 nt) investigated so far. However, the spliced intervening sequences are in some cases shifted in respect to the predicted ones. Moreover, we show that introns, if inserted into th...
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PMID: 15872156
PDF is available here.
PDF is available here.
Abstract
I elicitins, class III elicitins are thought to be cell-surface-anchored polypeptides. Molecular characterization of the inf2 genes indicated that they are widespread in Phytophthora spp. and occur as a small gene family. In addition, Southern blot and Northern blot hybridizations using gene-specifi...
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PMID: 15782632
PDF is available here.
PDF is available here.
Plant Physiology (137)2 2005
Abstract
We used two known Chlamydomonas reinhardtii nonphotosynthetic mutants carrying mutations in the Mcd1 nuclear gene (mcd1-1 and mcd1-2). We previously reported that these mutants fail to accumulate the chloroplast petD mRNA and its product, subunit IV of the cytochrome b6/f complex, which is essential...
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PMID: 15665248
PDF is available here.
PDF is available here.
Abstract
In this study, evidence for at least three independent losses of photosynthesis in the freshwater cryptophyte genus Cryptomonas is presented. The phylogeny of the genus was inferred by molecular phylogenetic analyses of the nuclear internal transcribed spacer 2 (nuclear ITS2), partial nuclear large...
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PMID: 15785847
PDF is available here.
PDF is available here.
Methods in Enzymology (395) 2005
Abstract
We present in this chapter a brief review of the progress that has been made in analyzing microalgae from populations to the species level. The described methods range from DNA fingerprinting techniques, such as random amplified polymorphic DNA (RAPD), amplified fragment length polymorphisms (AFLPs)...
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PMID: 15865974
PDF is available here.
PDF is available here.
Plant Cell Online (16)11 2004
Abstract
We used strain Delta26, in which atpB mRNA is unstable because of the lack of a 3' stem-loop structure. In this context, when a 121-nucleotide segment of atpB antisense RNA was expressed from an ectopic site, an elevated accumulation of atpB mRNA resulted. Finally, when spa19 was placed in a genetic...
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PMID: 15486097
PDF is available here.
PDF is available here.
Microbiological Research (159)4 2004
Abstract
A cDNA was cloned encoding ornithine decarboxylase (ODC) of the unicellular green alga Chlamydomonas reinhardtii. The polypeptide consists of 396 amino acid residues with 35-37% sequence identity to other eukaryotic ODCs. As indicated by the phylogenetic tree calculated by neighbour joining analysis...
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PMID: 15646386
PDF is available here.
PDF is available here.