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RNA, Messenger, Stored (0):
Recent article
RNA, Messenger, Stored
Development (137)22 2010
Abstract
We report that although Nv nanos (Nv-nos) has a conserved function in embryonic patterning through translational repression of hb, the timing and mechanisms of this repression are significantly delayed in the wasp compared with the fly. This delay in Nv-nos function appears to be related to the dyna...
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PMID: 20929949
PDF is available here.
PDF is available here.
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Biology of the Cell (102)9 2010
Abstract
DM1 (myotonic dystrophy type I) is a common form of muscular dystrophy that affects mainly adults. It is a disease that belongs to the group of defective RNA export diseases, since a major part of the pathogenic mechanism of the disease is the retention of the mutant transcripts in the cell nucleus....
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PMID: 20690904
PDF is available here.
PDF is available here.
Abstract
I discuss recent studies of the roles of miRNAs during the maternal-to-zygotic transition and cellular reprogramming, where they reshape transcriptional landscapes to facilitate the establishment of novel cellular states....
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PMID: 20452200
PDF is available here.
PDF is available here.
Development (137)10 2010
Abstract
We used loss- and gain-of-function experiments in the hover fly Episyrphus balteatus (Syrphidae) to address the question of how the maternal regulation of gap genes evolved. We find that, in Episyrphus, a highly diverged bicoid ortholog is solely responsible for the AP polarity of the embryo. Episyr...
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PMID: 20430746
PDF is available here.
PDF is available here.
Development (137)6 2010
Abstract
We show that Syntabulin, a linker of the kinesin I motor protein, is essential for dorsal determination in zebrafish. We found that syntabulin mRNA is transported to the vegetal pole during oogenesis through the Bucky ball (Buc)-mediated Balbiani body-dependent pathway, which is necessary for establ...
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PMID: 20150281
PDF is available here.
PDF is available here.
EMBO Journal (29)2 2010
Abstract
We show that the CPE does not direct early mRNA translation. Rather, early translation is directed through specific early factors, including the Musashi-binding element (MBE) and the MBE-binding protein, Musashi. Our findings indicate that although the cyclin B5 3' UTR contains both CPEs and an MBE,...
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PMID: 19959990
PDF is available here.
PDF is available here.
Abstract
We quantitatively compared mRNA among oocytes with different embryo development competence. A cytoplasm biopsy was removed from in vitro matured oocytes to perform comparative analysis of amounts of global polyadenylated (polyA) mRNA and housekeeping gene transcripts. After parthenogenetic activatio...
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PMID: 19393120
PDF is available here.
PDF is available here.
Abstract
We show that ectopic expression of the gamma-tubulin regulator, Shroom2, is sufficient to induce co-accumulation of pigment granules, spectrin, and dynactin in Xenopus blastomeres. Shroom2 and spectrin are enriched and co-localize specifically in the pigmented animal hemisphere of Xenopus eggs and b...
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PMID: 19554350
PDF is available here.
PDF is available here.
Abstract
We performed a modifier screen of the cdc-25.1(gf)-dependent hyperplasia. One of the isolated suppressor mutants possesses a donor splice site mutation in prp-8, a key splicing factor of the U5-specific snRNP. prp-8(rr40) produces aberrant prp-8 splice variants that generate C-terminal truncations a...
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PMID: 18945809
PDF is available here.
PDF is available here.
Abstract
We report that the zinc-finger protein Zelda (Zld; Zinc-finger early Drosophila activator) binds specifically to these sites and is capable of activating transcription in transient transfection assays. Mutant embryos lacking zld are defective in cellular blastoderm formation, and fail to activate ma...
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PMID: 18931655
PDF is available here.
PDF is available here.
Abstract
We previously showed that SMG destabilizes maternal Hsp83 mRNA by recruiting the CCR4-NOT deadenylase to trigger decay; however, the cis-acting elements through which this was accomplished were unknown. Here we show that Hsp83 transcript degradation is regulated by a major element, the Hsp83 mRNA in...
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PMID: 18794360
PDF is available here.
PDF is available here.
Abstract
We asked whether the Argonaute (Ago)/RNA-induced silencing complex, providing the mRNA "slicer" activity in gene silencing, might contribute to this process. Here we show that Ago2, 3, and 4 transcripts are contributed to the embryo maternally. By systematic knockdown of maternal Ago2, 3, and 4, ind...
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PMID: 18701707
PDF is available here.
PDF is available here.
Developmental Biology (321)1 2008
Abstract
We report that CDC2A (CDK1)-mediated phosphorylation of MSY2 triggers this transition. Injecting Cdc2a mRNA, which activates CDC2A, overcomes milrinone-mediated inhibition of oocyte maturation, induces MSY2 phosphorylation and the maturation-associated degradation of mRNAs. Inhibiting CDC2A followin...
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PMID: 18606161
PDF is available here.
PDF is available here.
Journal of Cell Biology (182)3 2008
Abstract
We find that repressed maternal mRNAs and their regulators localize to P body-like mRNP granules in the Caenorhabditis elegans germ line. Surprisingly, several distinct types of regulated granules form during oocyte and embryo development. 3' untranslated region elements direct mRNA targeting to one...
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PMID: 18695046
PDF is available here.
PDF is available here.
Journal of Cell Biology (182)3 2008
Abstract
We have investigated in Caenorhabditis elegans whether Dhh1 and Pat1 generally function together, and how they influence mRNA sequestration during oogenesis. We show that in somatic tissues, the Dhh1 orthologue (CGH-1) forms Pat1 (patr-1)-dependent P bodies that are involved in mRNA decapping. In co...
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PMID: 18695045
PDF is available here.
PDF is available here.
Genes & Development (22)12 2008
Abstract
We demonstrate, by using conditional knockout mice, that the other known DNA methyltransferases Dnmt3a and Dnmt3b are dispensable for the maintenance of the methylation marks at most imprinted loci. We further demonstrate that a lack of both maternal and zygotic Dnmt1 results in complete demethylati...
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PMID: 18559477
PDF is available here.
PDF is available here.
Differentiation (76)5 2008
Abstract
We test a new, in vivo-in vitro approach based on the hypothesis that calvarial suture cells passaged in minimal medium exhibit a stable gene expression profile similar to undifferentiated osteoblastic cells that can provide a benchmark for comparison with in vivo expression of differentiated tissue...
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PMID: 18093228
PDF is available here.
PDF is available here.
Current Biology (18)7 2008
Abstract
We report that a cluster of zygotically expressed microRNAs (miRNAs) targets maternal mRNAs for turnover, as part of the zygotic degradation pathway. miRNAs are small noncoding RNAs that silence gene expression by repressing translation of their target mRNAs and by promoting mRNA turnover. Intriguin...
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PMID: 18394895
PDF is available here.
PDF is available here.
Abstract
During fertilization, spermatozoa contribute genetic and epigenetic factors that affect early embryogenesis. Genetic factors include a haploid genome with intact coding regions and regulatory regions for essential genes. The DNA must contain the proper copy number of essential genes, and cannot have...
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PMID: 18413055
PDF is available here.
PDF is available here.
Abstract
We report the first study of gene expression and protein localization of the Cbx genes in in vitro produced bovine embryos. All but one gene (Cbx6) were expressed. This was confirmed by immunolocalization for HP1alpha, beta, gamma, and Pc2, 3. HP1beta was found in the nuclei of embryos from the two-...
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PMID: 17486619
PDF is available here.
PDF is available here.
Abstract
We show that the number and relative position of two elements, the CPE and the Pumilio-binding element, with respect to the polyadenylation signal define a combinatorial code that determines whether an mRNA will be translationally repressed by CPEB, as well as the extent and time of cytoplasmic poly...
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PMID: 18267074
PDF is available here.
PDF is available here.
Abstract
Here we further assess imprinted gene expression and DNA methylation at different cluster types in order to discern patterns. These data reveal POxBW misexpression at the Kcnq1ot1 and Peg3 clusters, both of which lose ICR methylation in placental tissues. In contrast, some embryonic transcripts (Peg...
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PMID: 18958286
PDF is available here.
PDF is available here.
Abstract
We report that: 1) the transcription factor Oct-4 is absent in MIINSN oocytes, accounting for 2) the down-regulation of Stella, a maternal-effect factor required for the oocyte-to-embryo transition and of which Oct-4 is a positive regulator; 3) eighteen Oct-4-regulated genes are up-regulated in MIIN...
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PMID: 18837968
PDF is available here.
PDF is available here.
Abstract
We studied zygotic gene expression in nine inbred lines of Drosophila melanogaster at two time points in their development. We find abundant variation for transcript level both between lines and over time; close to half of all expressed genes show a significant line effect at either time point. We e...
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PMID: 19021738
PDF is available here.
PDF is available here.
Abstract
We describe the methodology for targeting of maternal mRNAs and for successful fertilization of mRNA-depleted oocytes....
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PMID: 19109723
PDF is available here.
PDF is available here.
Abstract
We will focus on the production and reversible silencing of maternal mRNAs during oogenesis, their asymmetric activation after fertilization, and their subsequent clearance at the midblastula transition. Where possible, insights from mechanistic studies are presented....
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PMID: 18365862
PDF is available here.
PDF is available here.
Abstract
We show that particular GAL4 drivers transiently direct ectopic expression of UAS-linked transgenes at early stages of embryogenesis in a GAL4- dependent manner via a mechanism that involves parental transmission of Gal4 transcripts.
(c) 2007 Wiley-Liss, Inc....
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PMID: 18064670
PDF is available here.
PDF is available here.
Developmental Biology (311)2 2007
Abstract
We identified and functionally characterized a novel vegetally localizing mRNA encoding a glutamate receptor interacting protein (GRIP) family member in Xenopus, termed XGRIP2.1. XGRIP2.1 is specifically associated with the germ plasm and PGCs throughout Xenopus embryogenesis. Morpholino-mediated kn...
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PMID: 17936745
PDF is available here.
PDF is available here.
Development (134)22 2007
Abstract
We thus identify a precise combinatorial code of maternal factors responsible for zygotic onset of a chordate ectodermal genetic program....
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PMID: 17965050
PDF is available here.
PDF is available here.
Physiological Genomics (31)2 2007
Abstract
Our analysis revealed 419 genes that were expressed in both oocytes and blastocysts. The expression of 1,324 genes was detected exclusively in the blastocyst, in contrast to 164 in the oocyte including a significant number of novel genes. Genes indicative for transcriptional and translational contro...
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PMID: 17595343
PDF is available here.
PDF is available here.
EMBO Journal (26)17 2007
Abstract
We studied the function of TATA-binding protein (TBP) by knock down and DNA microarray analysis of gene expression in early embryo development. We show that a subset of polymerase II-transcribed genes with ontogenic stage-dependent regulation requires TBP for their zygotic activation. TBP is also re...
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PMID: 17703193
PDF is available here.
PDF is available here.
Theriogenology (68 Suppl 1) 2007
Abstract
Recently it has been demonstrated that, along with sperm, some of its RNA can be introduced into the oocyte during fertilization, which stays stable until the activation of the embryonic genome. Originally it was thought that RNA present in semen relates to contamination from somatic cells and/or im...
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PMID: 17583784
PDF is available here.
PDF is available here.
Theriogenology (68 Suppl 1) 2007
Abstract
Messenger RNA (mRNA) expression techniques have become a powerful tool to analyze the relative abundance of transcripts related to oocyte and/or embryo quality. Numerous efforts to identify candidate genes for the developmental competence of bovine oocytes and embryos have been made employing differ...
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PMID: 17524469
PDF is available here.
PDF is available here.
Theriogenology (68 Suppl 1) 2007
Abstract
We have carried out a global mRNA gene expression analysis of bovine oocytes pre- and post-resumption of meiotic maturation using the Affymetrix GeneChip Bovine Genome Array. Approximately 54% of the probe sets representing 23,000 transcripts were detected in bovine oocytes. Of which, 821 transcript...
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PMID: 17512044
PDF is available here.
PDF is available here.
Genes to Cells (12)7 2007
Abstract
We report the identification of the Xenopus laevis ortholog of human FANCG (xFANCG), its expression during development, and its molecular interactions with a partner protein, xFANCA. The xFANCG protein sequence is 47% similar to its human ortholog, with highest conservation in the two putative N-ter...
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PMID: 17584296
PDF is available here.
PDF is available here.
Current Biology (17)12 2007
Abstract
The complex cleavage pattern in ascidian embryos can be explained by a simple rule of centrosome attraction mediated by localized PEM activity. PEM is the first gene identified in ascidians that is required for multiple spindle-positioning events....
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PMID: 17570671
PDF is available here.
PDF is available here.
Pediatrics International (49)3 2007
Abstract
The present finding is in contrast to that of most previous reports that indicated a higher incidence of UPD in PWS. It is unclear whether this discrepancy in the incidence of UPD arises from under-diagnosis or because of ethnic differences, a question worthy of further study. The three-phase screen...
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PMID: 17532839
PDF is available here.
PDF is available here.
Abstract
We report the identification and cloning of a Trypanosoma cruzi Dhh1 homolog gene. TcDhh1 expression is not regulated through the parasite life cycle or under stress conditions. We show that TcDhh1 is present in polysome-independent complexes and is localized to discrete cytoplasmic foci, resembling...
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PMID: 17399688
PDF is available here.
PDF is available here.
Abstract
Maternal gene products drive early development when the newly formed embryo is transcriptionally inactive. During the maternal-zygotic transition, embryonic transcription is initiated and many maternal RNAs are degraded. Multiple mechanisms regulate the birth of zygotic RNAs and the death of materna...
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PMID: 17446392
PDF is available here.
PDF is available here.
Abstract
We suggest that the meiotic machinery functions as an internal pacemaker that propels oocytes toward embryogenesis....
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PMID: 17446393
PDF is available here.
PDF is available here.
Abstract
We show that bcd's permissive function is mimicked in the wasp by a maternal repression system in which anterior localization of the wasp ortholog of giant represses anterior expression of the trunk gap genes so that head and thorax can properly form....
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PMID: 17395827
PDF is available here.
PDF is available here.
Molecular Cell (25)5 2007
Abstract
Recent results indicate that many untranslating mRNAs in somatic eukaryotic cells assemble into related mRNPs that accumulate in specific cytoplasmic foci referred to as P bodies. Transcripts associated with P body components can either be degraded or return to translation. Moreover, P bodies are al...
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PMID: 17349952
PDF is available here.
PDF is available here.
Annals of Epidemiology (17)2 2007
Abstract
These findings indicate that use of perinatal characteristics as surrogates for hormone levels should be limited to a specific ethnic group. For white men, previously reported associations of TGCT with maternal weight and age may be caused by lower maternal testosterone levels....
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PMID: 16882463
PDF is available here.
PDF is available here.
XSu(H)2 is an essential factor for gene expression and morphogenesis of the Xenopus gastrula embryo.
Abstract
We analyzed the function of Xenopus Suppressor of Hairless (XSu(H)) using morpholino antisense oligonucleotides (MO), which interfere with the translation of transcripts. In Xenopus embryos, maternal transcripts of both XSu(H)1 and XSu(H)2 were ubiquitously observed until the blastula stage and ther...
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PMID: 17183462
PDF is available here.
PDF is available here.
Developmental Biology (300)1 2006
Abstract
We learned a test for allelism in genetics class that bore that Latin name but now it comes as a new day for biological science-a welcome era in which a phenomenon as complex as development can be envisioned from principles of chemical binding energy and specificity. The buzzword (the term is just-a...
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PMID: 17113576
PDF is available here.
PDF is available here.
Developmental Biology (299)2 2006
Abstract
We have cloned the zebrafish orthologue. We present here the gene, domain structure, and expression pattern of zebrafish cypher during development. Cypher was not present as a maternal mRNA and was absent during early development. Cypher mRNA was first detected at the 3-somite stage in adaxial somit...
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PMID: 16982050
PDF is available here.
PDF is available here.
Current Biology (16)20 2006
Abstract
We show that d4EHP, a cap binding protein that represses caudal (cad) mRNA translation , also inhibits hb mRNA translation by interacting simultaneously with the mRNA 5' cap structure (m(7)GpppN, where N is any nucleotide) and Brat. Thus, by regulating Cad and Hb expression, d4EHP plays a key role i...
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PMID: 17055983
PDF is available here.
PDF is available here.
Abstract
We show that, after induction during the primary response, high CCL5 mRNA levels are specifically preserved in CD8 T cells. We have investigated the mechanisms involved in the long-term maintenance of CCL5 mRNA levels by memory CD8 T cells. We demonstrate that the CCL5 mRNA half-life is increased in...
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PMID: 16983723
PDF is available here.
PDF is available here.
Genes & Development (20)19 2006
Abstract
These results implicate conserved pathways underlying transition from oogenesis to initiation of development and illustrate how genes acquire and lose reproductive functions during evolution, a potential mechanism for reproductive isolation....
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PMID: 17015433
PDF is available here.
PDF is available here.
Developmental Biology (298)1 2006
Abstract
We present a comprehensive analysis of changes in the maternal component of the zygotic polysomal mRNA population during the transition from oocyte to late one-cell stage embryo. We observe global transitions in the functional classes of translated MmRNAs and apparent changes in the underlying cis-r...
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PMID: 16860309
PDF is available here.
PDF is available here.
Mammalian Genome (17)9 2006
Abstract
We compared published results of three independent studies of the mouse preimplantation embryo transcriptome, each performed in a different laboratory using different microarray platforms. We searched the combined data set for genes whose expression patterns were consistent among the three experimen...
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PMID: 16964442
PDF is available here.
PDF is available here.
Abstract
Translational repression of messenger RNAs (mRNAs) plays an important role in sexual differentiation and gametogenesis in multicellular eukaryotes. Translational repression and mRNA turnover were shown to influence stage-specific gene expression in the protozoan Plasmodium. The DDX6-class RNA helica...
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PMID: 16888139
PDF is available here.
PDF is available here.
Abstract
Genes & Development (20)13 2006
Abstract
We generated a maternal-effect mutation of Brg1, which encodes a catalytic subunit of SWI/SNF-related complexes, utilizing Cre-loxP gene targeting. In conditional-mutant females, BRG1-depleted oocytes completed meiosis and were fertilized. However, embryos conceived from BRG1-depleted eggs exhibited...
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PMID: 16818606
PDF is available here.
PDF is available here.
Abstract
We conducted a degenerated oligonucleotide polymerase chain reaction (PCR) to screen a mouse embryonic stem (ES) cell cDNA library. A novel homeobox-containing gene, Eso-1, which is preferentially expressed in ES cells and ovaries, was identified. The full-length Eso-1 cDNA was found to be 1,710 bp...
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PMID: 16596637
PDF is available here.
PDF is available here.
Development (133)14 2006
Abstract
We found that the postplasm-containing blastomeres, the B7.6 cells, undergo an asymmetric cell division during gastrulation to produce two distinct daughter cells: B8.11 and B8.12. Most of the postplasmic components segregate only into the B8.11 cells, which never coalesce into the gonad. By contras...
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PMID: 16794033
PDF is available here.
PDF is available here.
Genes & Development (20)10 2006
Abstract
We prematurely truncated the Kcnq1ot1 transcript by inserting a transcriptional stop signal downstream of the promoter. We show that the lack of a full-length Kcnq1ot1 transcript on the paternal chromosome leads to the expression of genes that are normally paternally repressed. Finally, we demonstra...
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PMID: 16702402
PDF is available here.
PDF is available here.
Developmental Biology (291)1 2006
Abstract
We show that Sm proteins also regulate germ cell specification during early C. elegans embryogenesis. SmE and SmG were required to maintain transcriptional quiescence in embryonic germ cell precursors. In addition, depletion of SmE inhibited expression of the germ lineage-specific proteins PIE-1, GL...
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PMID: 16413530
PDF is available here.
PDF is available here.
Abstract
We investigated the functions of five of the nine Type I mRNAs so far known in Halocynthia roretzi: Hr-Wnt-5, Hr-GLUT, Hr-PEM3, Hr-PEN1, and Hr-PEN2. Suppression of their functions with specific antisense morpholino oligonucleotides (MOs) had effects on the formation of various tissues: Hr-Wnt-5 on...
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PMID: 16369806
PDF is available here.
PDF is available here.