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RNA, Nuclear (2):
Recent article
RNA, Nuclear
Biology of the Cell (102)9 2010
Abstract
DM1 (myotonic dystrophy type I) is a common form of muscular dystrophy that affects mainly adults. It is a disease that belongs to the group of defective RNA export diseases, since a major part of the pathogenic mechanism of the disease is the retention of the mutant transcripts in the cell nucleus....
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PMID: 20690904
PDF is available here.
PDF is available here.
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Journal of Virology (84)13 2010
Abstract
Although the viral Rev protein is necessary for HIV replication, its main function in the viral replication cycle has been controversial. Reinvestigating the effect of Rev on the HIV-1 RNA distribution in various cell lines and primary cells revealed that Rev enhanced cytoplasmic levels of the unspl...
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PMID: 20427541
PDF is available here.
PDF is available here.
Abstract
It often is desirable to "prefractionate" RNA before analysis. Ordinarily, this can only be done with tissue culture cells, although it is possible to isolate nuclei and cytoplasm from certain "soft" tissues such as liver and white blood cells. This protocol describes a method for separating nuclei...
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PMID: 20516179
PDF is available here.
PDF is available here.
Abstract
We attempt to overcome these pitfalls by combining several methods suggested in previous studies and routinely used in contemporary standard phylogenetic analyses but that have not yet been applied to any bilaterian phylogeny based on these genes. The methods used include maximum likelihood and Baye...
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PMID: 19129141
PDF is available here.
PDF is available here.
Experimental Cell Research (315)2 2009
Abstract
We applied a proteomic approach along with a Southwestern assay to detect both differentially expressed and MAR-binding NM proteins, in persistent hepatocyte nodules (PHN) in respect with normal hepatocytes (NH). In PHN, the NM undergoes changes both in morphology and in protein composition. We dete...
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PMID: 19000672
PDF is available here.
PDF is available here.
Abstract
Journal of Parasitology (94)4 2008
Abstract
I gene of Leptorhynchoides thecatus and Pseudoleptorhynchoides lamothei were used in phylogenetic analyses with available sequences of 26 other acanthocephalans. Maximum parsimony and maximum likelihood analyses were identical in placing both genera in the Illiosentidae. Bootstrap analyses also indi...
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PMID: 18576776
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1779)4 2008
Abstract
We will discuss potential mechanisms of TRAMP recruitment and exosome activation during RNA surveillance and degradation. Less well-understood observations link both the TRAMP and exosome complexes to chromatin structure and DNA repair, and we will speculate on the potential significance of these ac...
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PMID: 18211833
PDF is available here.
PDF is available here.
Abstract
We analyze patterns of global gene expression in cells lacking the Cid14 or the Dis3/Rpr44 subunit of the nuclear exosome. We found that transcripts from many genes induced during meiosis, including key regulators, accumulated in the absence of Cid14 or Dis3. Moreover, our data suggest that addition...
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PMID: 18025105
PDF is available here.
PDF is available here.
Abstract
Our knowledge of mRNA export mechanisms in flowering plants is in its infancy. Some proteins of the NUP107-160 complex, NUPs and DEAD-box proteins (DBPs), have been studied in flowering plants. Arabidopsis NUP160/SAR1 plays a critical role in mRNA export, regulation of flowering, and hormone and abi...
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PMID: 18630756
PDF is available here.
PDF is available here.
Abstract
We revealed firstly a decrease in the isoelectric point range of nuclear soluble proteins, which are known to be actively engaged in nuclear RNA metabolism, and a shortening in the molecular weight range of them under clinorotation. Moreover, minor and major proteins in clinorotated samples had decr...
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PMID: 18372723
PDF is available here.
PDF is available here.
BMC Genomics (8)1 2007
Abstract
We used a combination of in silico comparative genomics and microarray-based transcriptional profiling. This approach to screening identified ~60 intergenic regions conserved between Sinorhizobium meliloti and related members of the alpha-proteobacteria sub-group 2. Of these, 14 appear to correspond...
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PMID: 18093320
PDF is available here.
PDF is available here.
Planta Medica (72)9 2006
Abstract
The nuclear 18S rRNA and chloroplast MATK genes of 18 samples of Panax notoginseng and its processed material Sanqi (Radix Notoginseng) were analyzed. The two genes, regardless of cultivar origin, were found to be identical to genotype R1 and M1, respectively, of the published sequences (GenBank acc...
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PMID: 16881018
PDF is available here.
PDF is available here.
Journal of Ethnopharmacology (106)2 2006
Abstract
Astragalus membranaceus (Fisch.) Bunge, a commonly used Chinese medicinal material, from certain localities contains more favourable trace elements and fewer harmful trace elements than those from other localities. Therefore, there is a need to distinguish Astragalus membranaceus from different loca...
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PMID: 16442761
PDF is available here.
PDF is available here.
Abstract
Microarray analyses were performed on yeast strains mutant for the nuclear-specific exosome components Rrp6p and Rrp47p/Lrp1p or the core component Rrp41p/Ski6p, at permissive temperature and following transfer to 37 degrees C. 339 mRNAs showed clearly altered expression levels, with an unexpectedly...
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PMID: 16652390
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1759)8-9 2006
Abstract
We used high-density microarrays to compare nuclear and cytoplasmic RNA levels from untreated and TCDD-treated mouse embryonic fibroblasts (MEF) to test the hypotheses that (1) TCDD has a large impact on nuclear RNA levels and (2) that cytoplasmic RNA levels are dependent on nuclear RNA levels. We f...
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PMID: 16962184
PDF is available here.
PDF is available here.
Tsitologiia (48)5 2006
Abstract
We continue studies for identifying the molecular weight and chemical nature of the TSPC, and the role of its different fractions in regulation of proliferation processes. Besides, it is planned to produce antibodies against TSPC fractions with the purpose to block its inhibitory effect on myocyte r...
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PMID: 16892854
PDF is available here.
PDF is available here.
Abstract
We still do not have detailed enough information to understand fully the organization and regulation of the various processes taking place in the nucleolus. However, the present power of light and electron microscopy (EM) techniques means that a description of nucleolar processes at the molecular le...
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PMID: 17178467
PDF is available here.
PDF is available here.
Circulation Research (97)11 2005
Abstract
We report evidence for a parallel mechanism in the heart. Accumulation of expanded poly(CUG) RNA in nuclear foci is associated with sequestration of muscleblind proteins and abnormal regulation of alternative splicing in DM1 cardiac muscle. A toxic effect of RNA with an expanded repeat may contribut...
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PMID: 16254211
PDF is available here.
PDF is available here.
Abstract
In this issue of Cell, Prasanth et al. (2005) provide evidence that an inosine-containing RNA that is normally retained in the nucleus is cleaved within its 3' untranslated region following cellular stress. It is then transported to the cytoplasm and translated into protein. These findings suggest th...
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PMID: 16239133
PDF is available here.
PDF is available here.
Abstract
We show that contrary to prevailing notion, a group of nucleoplasmic proteins, mostly RNA binding proteins, relocalized from the nucleoplasm to a specific nucleolar cap during transcriptional inhibition. For instance, an exclusively nucleoplasmic protein, the splicing factor PSF, localized to nucleo...
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PMID: 15758027
PDF is available here.
PDF is available here.
FASEB Journal (19)6 2005
Abstract
The relatively low levels of transfection that can be achieved by current gene delivery systems have limited the therapeutic utility of gene transfer. This is especially true for non-viral gene delivery systems, where the levels of gene expression achieved are usually well below the levels achieved...
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PMID: 15677338
PDF is available here.
PDF is available here.
Experimental Cell Research (302)2 2005
Abstract
We also studied the RNA distribution from combinations of neighboring HIV and bacterial resistance genes that co-integrated within the genome of COS-7 cells-revealing spherical or track-like accumulations of RNA that were extensively branched. There were many nuclei with distinct but overlapping RNA...
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PMID: 15561100
PDF is available here.
PDF is available here.
Abstract
Since the chromanol ring is shared by both tocopherols and tocotrienols and the only difference between these two subgroups of Vitamin E is the phytol side chain, we conclude that the polyunsaturated phytol group in tocotrienols plays a key preventive role in E2 epoxidation. This is the first report...
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PMID: 16055275
PDF is available here.
PDF is available here.
Abstract
We examined the efficacy of expression-library immunization using a T. taeniaeformis rat model system. Total RNAs were isolated from the last 15 segments of adult T. taeniaeformis and poly A RNA was purified. cDNA library was produced using SuperScript Plasmid System, which contains a mammalian expr...
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PMID: 16438198
PDF is available here.
PDF is available here.
Experimental Parasitology (109)1 2005
Abstract
We have found differences in sympatric tick populations. Notable differences were found in the body size and weight of Dermacentor andersoni collected from a single location in Montana, USA. These ticks were separated in groups consisting of big (B) and small (S) individuals. The objectives of this...
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PMID: 15639135
PDF is available here.
PDF is available here.
Abstract
We previously reported that sonic hedgehog (Shh) induces the differentiation of rat ventral forebrain neurons expressing a novel marker, EVF-1 [Development 125 (1998) 5079]. In this report, we show that EVF-1 is a novel, developmentally regulated, non-coding RNA, with no homology to other known non-...
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PMID: 15183307
PDF is available here.
PDF is available here.
Mechanisms of Ageing and Development (125)10-11 2004
Abstract
We have found no activation of this transcription factor in the brains of old (36 months) rats in response to exposure to 41 degrees C for 1h while strong activation is elicited in young (6 months) animals. Since brains of young and old rats had approximately the same amount of HSF-1 subunits, we an...
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PMID: 15541771
PDF is available here.
PDF is available here.
Abstract
X chromosome inactivation begins when a novel chromosomal RNA (cRNA) from the imprinted mouse Xist or human XIST locus coats or "paints" one X chromosome in cis and initiates a cascade of chromosome remodeling events. Molecular cytological studies have proven invaluable for understanding the distinc...
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PMID: 15015744
PDF is available here.
PDF is available here.
Plant Cell Online (15)11 2003
Abstract
We report here the differential subnuclear localization of RNA strands of opposite polarity derived from the replicating Potato spindle tuber viroid (PSTVd). During replication, (+)- and (-)-strand viroid RNAs are produced. We found that in infected cultured cells and plants, the (-)-strand RNA was...
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PMID: 14555700
PDF is available here.
PDF is available here.
Abstract
We found two main groups of gorgonian octocorals using a maximum parsimony analysis of the two genes. One group corresponds to deep-water taxa including species from the suborders Calcaxonia and Scleraxonia characterized by an enlargement of the G13 helix. The second group has species from Alcyoniin...
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PMID: 12967605
PDF is available here.
PDF is available here.
Abstract
We show here that only a fraction of LRP130 proteins are in nuclei and are directly bound in vivo to at least some of the same RNA molecules as the nucleocytoplasmic shuttle protein hnRNP A1. The majority of LRP130 proteins are located within mitochondria, where they are directly bound to polyadenyl...
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PMID: 12832482
PDF is available here.
PDF is available here.
Abstract
We used a well-established fluorescence in situ hybridization technique which allows one to simultaneously detect pre-mRNAs and assess ploidy level in a single cell. All alleles of GPIIb, GPIIIa, VWF, beta-actin, hsp70, c-mpl, Fli-1, and FOG-1 genes are transcriptionally active in megakaryocytes fro...
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PMID: 12393414
PDF is available here.
PDF is available here.
Genome Research (12)12 2002
Abstract
We have measured, by reverse transcription and real-time quantitative PCR, the steady-state levels of the mitochondrial and nuclear transcripts encoding several subunits of the human oxidative phosphorylation (OXPHOS) system, in different normal tissues (muscle, liver, trachea, and kidney) and in cu...
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PMID: 12466294
PDF is available here.
PDF is available here.
Plant Physiology (130)3 2002
Abstract
We recently characterized a new Arabidopsis mutant, abh1, which shows ABA-hypersensitive regulation of seed germination, stomatal closing, and cytosolic calcium increases in guard cells (V. Hugouvieux, J.M. Kwak, J.I. Schroeder [2001] Cell 106: 477-487). ABH1 encodes the large subunit of a dimeric A...
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PMID: 12427994
PDF is available here.
PDF is available here.
Transplantation (73)9 2002
Abstract
By in situ hybridization, expression of the RNA product, homologous for EBV-encoded RNA (EBER) was identified in the PTLD tumor cells of all cases, indicating latent primate EBV- related infection. This report identifies a novel animal model of EBV associated PTLD in the setting of kidney transplant...
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PMID: 12023621
PDF is available here.
PDF is available here.
Abstract
In mammalian cells, significantly more RNA is turned over in the nucleus than in the cytoplasm. However, only recently have we begun to understand the mechanisms and regulation of nuclear RNA decay.
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PMID: 11909514
PDF is available here.
PDF is available here.
Genetic engineering (24) 2002
Abstract
We studied tRNA import in the yeast system, both in vitro and in vivo, and applied similar approaches to study 5S rRNA import into human mitochondria. Despite the obvious divergence of RNA import systems suggested for different species, we find that in yeast and human cells this pathway involves sim...
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PMID: 12416306
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (74)3 2002
Abstract
The model of partial hepatectomy was utilized to investigate the influence of human fetal liver cells (FLCs) and of human embryo tissue (PCF) postnuclear cytoplasm fraction injection on the rate of DNA and nRNA synthesis in regenerating rat liver cells. Single infusion of FLCs and PCF into the splee...
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PMID: 12916234
PDF is available here.
PDF is available here.
Molecular Brain Research (96)1-2 2001
Abstract
We tested the hypothesis that rapid, stress-induced CRH transcription occurred during the first two postnatal weeks, and is associated with pCREB expression. The time-course of induction of unedited, heteronuclear CRH RNA (CRH hnRNA) was examined in hypothalamic paraventricular nucleus (PVN) of imma...
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PMID: 11731007
PDF is available here.
PDF is available here.
Abstract
Analyses of CYP1A1 mRNA were used to monitor the responsiveness of murine hepatoma 1c1c7 and human monocytic U937 cells in different phases of the cell cycle to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Concentrations of TCDD capable of inducing CYP1A1 were not cytostatic to either cell line. Stea...
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PMID: 11602686
PDF is available here.
PDF is available here.
Abstract
These results may be explained by exon-specific nuclear RNA degradation rates, as also indicated by results from an in vitro degradation assay using a HeLa nuclear extract.
Copyright 2001 Academic Press....
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PMID: 11485396
PDF is available here.
PDF is available here.
Molecular Pharmacology (59)5 2001
Abstract
We conclude that P450R transcription, rather than nuclear RNA processing or mRNA stabilization, is the primary mechanism whereby T3 induces hepatic P450R mRNA....
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PMID: 11306680
PDF is available here.
PDF is available here.
Arthritis & Rheumatism (44)5 2001
Abstract
CDDO selectively reduced the induction of MMP-1 and MMP-13 at the levels of messenger RNA and protein. Treatment with CDDO prior to cytokine stimulation enhanced this inhibition, and we demonstrated that CDDO functions at the level of transcription. Additionally, CDDO reduced IL-1beta-mediated invas...
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PMID: 11352241
PDF is available here.
PDF is available here.
Abstract
We have examined the expression of RAG genes in 11 cases of EBV-associated endemic Burkitt's lymphoma (BL), 25 cases of Hodgkin's disease (HD, 17 EBV(+), 8 EBV(-)) and 10 cases of follicular non-Hodgkin's lymphoma (NHL). Using in situ hybridization, expression of the RAGs was detected in cortical th...
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PMID: 11279609
PDF is available here.
PDF is available here.
Abstract
The mitochondrion-associated RNase P activity (mtRNase P) was extensively purified from HeLa cells and shown to reside in particles with a sedimentation constant ( approximately 17S) very similar to that of the nuclear enzyme (nuRNase P). Furthermore, mtRNase P, like nuRNase P, was found to process...
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PMID: 11134342
PDF is available here.
PDF is available here.
Abstract
The nucleolar and extranucleolar RNA synthesis was studied in the mink blastocysts at different stages of embryonic diapause and during the periimplantation period using cytoradioautography. The data obtained suggest a differential and stage specific activity of the embryonic mRNA and rRNA synthesis...
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PMID: 11573428
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (275)39 2000
Abstract
We have found that alpha-globin pre-mRNA is stabilized by a mechanism that does not modulate the half-life of mature mRNA. The insertion of DNA fragments of various unrelated sequences into the 3'-UTR of the human alpha-globin gene strongly reduces mRNA abundance upon transfection into choriocarcino...
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PMID: 10867002
PDF is available here.
PDF is available here.
Journal of Cell Biology (149)3 2000
Abstract
We found that human methionyl-tRNA synthetase (MRS) was translocated to nucleolus in proliferative cells, but disappeared in quiescent cells. The nucleolar localization of MRS was triggered by various growth factors such as insulin, PDGF, and EGF. The presence of MRS in nucleoli depended on the inte...
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PMID: 10791971
PDF is available here.
PDF is available here.
Abstract
We really know about the nonchromatin elements (if any) of internal nuclear structure? Are there objective reasons (as opposed to thinly veiled disdain) to question experiments that use harsh nuclear extraction steps and precipitation-prone conditions? Are the known biophysical properties of the nuc...
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PMID: 10712500
PDF is available here.
PDF is available here.
Journal of Cell Biology (148)2 2000
Abstract
We used an analytical transmission electron microscopic method to determine the structure and composition of PML nuclear bodies and the surrounding nucleoplasm. Electron spectroscopic imaging (ESI) demonstrates that the core of the PML nuclear body is a dense, protein-based structure, 250 nm in diam...
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PMID: 10648561
PDF is available here.
PDF is available here.
Abstract
Abstract
Our findings support the notion that the nucleolus is the site of assembly and/or interaction between the family of ribonucleoproteins involved in protein synthesis, in addition to ribosomes themselves....
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PMID: 10618370
PDF is available here.
PDF is available here.
Abstract
The nucleolar localization elements (NoLEs) of U17 small nucleolar RNA (snoRNA), which is essential for rRNA processing and belongs to the box H/ACA snoRNA family, were analyzed by fluorescence microscopy. Injection of mutant U17 transcripts into Xenopus laevis oocyte nuclei revealed that deletion o...
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PMID: 10564278
PDF is available here.
PDF is available here.
FEBS Letters (457)2 1999
Abstract
We present evidence for an association between a splicing factor, hnRNP-F, and TATA-binding protein (TBP), which is an essential factor needed for transcription initiation. An affinity detection experiment revealed hnRNP-F in the preparation of TBP-interacting proteins. HnRNP-F was associated with T...
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PMID: 10471789
PDF is available here.
PDF is available here.
Abstract
These results imply that T. brucei possesses distinctive pathways for its nuclear RNA metabolism, which is consistent with a spatio-functional organization of the parasite's nucleus.
Copyright 1999 Academic Press....
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PMID: 10425153
PDF is available here.
PDF is available here.
FEBS Letters (452)1-2 1999
Abstract
We summarize our current knowledge and recent progress in identifying components involved in nuclear RNA export in yeast....
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PMID: 10376682
PDF is available here.
PDF is available here.
Abstract
We describe a method to image selectively the protein-based architecture in the eukaryotic cell nucleus using nitrogen and phosphorus mapping. In addition, we describe a method to determine total mass as well as stoichiometric relationships between protein and RNA. This method is illustrated using p...
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PMID: 10420641
PDF is available here.
PDF is available here.
Abstract
We examined another aspect of transcriptional regulation, intergenic transcription, which has been observed throughout the prototypic gene cluster of human beta-globin. These intergenic transcripts are nuclear and it is possible that they play an important functional role in the beta-globin locus. H...
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PMID: 10049749
PDF is available here.
PDF is available here.
Journal of Physiology (515 ( Pt 1)) 1999
Abstract
1. The contributions of specific residues in gamma- and epsilon-subunits to the developmental changes in conductance and open time of Xenopus muscle acetylcholine receptors (AChRs) were investigated. This study was directed primarily at residues in the M2 domains of gamma- and epsilon-subunits; howe...
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PMID: 9925875
PDF is available here.
PDF is available here.