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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Nucleic Acids (4):
Recent article
Nucleic Acids
Abstract
Gene therapy holds the promise of correcting a genetic defect. It can be achieved with the introduction of a normal wild-type transgene into specific cells of the patient where the endogenous gene is underexpressing or by the introduction of a therapeutic agent, such as, antisense ol...
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PMID: 21291381
PDF is available here.
PDF is available here.
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Analytical Biochemistry (411)1 2011
Abstract
Guanidinium thiocyanate-phenol-chloroform extraction (GTPC extraction) is widely used in molecular biology for isolating DNA, RNA, and proteins. Protein isolation by commercially available GTPC solutions is time consuming and the resulting pellets are only incompletely soluble. In th...
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PMID: 21094121
PDF is available here.
PDF is available here.
Abstract
We present an overview of the main results arrived at by the application of single-molecule methods to the study of the main machines of the central dogma.
Copyright © 2011 Elsevier Inc. All rights reserved....
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PMID: 21335233
PDF is available here.
PDF is available here.
Abstract
The concept of using nucleic acids as a prospective antiviral therapeutics has been one of the major areas of study for researchers since the last three decades. Among the many proposed technologies for specifically perturbing gene expression, four potent nucleic acids based technolo...
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PMID: 20738240
PDF is available here.
PDF is available here.
Abstract
The conference Applications of Nucleic Acids Technologies in Molecular Diagnostics (part of TIDES) consisted of four sessions: Regulatory Pathways and Quality Strategies, Manufacturing and Business Considerations for Successful Launch in the Clinical Market, Analytical Methods and Validation, and Ne...
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PMID: 20964604
PDF is available here.
PDF is available here.
Kidney International (78)10 2010
Abstract
Kidney International (78)10 2010
Abstract
Abstract
We compared the efficiencies of biosensors that use gold-coated single-crystal silicon and amorphous glass substrates. The reflectivity of light over a wide range of wavelengths was higher from gold layer coated single-crystal silicon substrates than from glass substrates. Furthermore, the efficienc...
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PMID: 20628872
PDF is available here.
PDF is available here.
Abstract
We previously showed the feasibility of using locked nucleic acid (LNA) for flow cytometric-fluorescence in situ hybridization (LNA flow-FISH) detection of a target cellular mRNA. Here we demonstrate how the method can be used to monitor viral RNA in infected cells. We compared the results of the LN...
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PMID: 20584898
PDF is available here.
PDF is available here.
Abstract
Exposure to fumonisins (FB) is known to have toxic and carcinogenic effects in different animal species, and to express toxicity in cells via the induction of oxidative stress. The aim of the current study was to evaluate the protective effects of the ethanol extract of Aquilegia vulgaris L. against...
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PMID: 20230848
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)8 2010
Abstract
We report a label-free fluorescence turn-on approach for the selective sensing of potassium. A properly selected G-rich oligonucleotide (oligo-Y) folded into stable quadruplex structure when mixed with potassium in an aqueous solution. Single-stranded nucleic acid specific nuclease was subsequently...
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PMID: 20585688
PDF is available here.
PDF is available here.
Analyst (Cambridge UK) (135)8 2010
Abstract
We have developed a Cyclic Enzymatic Amplification Method (CEAM) for sensitive and selective detection of nucleic acids. In this design, the displacing probe is non-fluorescent on its own and cannot be digested by Exo III until displacement hybridization by a target sequence, leading to release of f...
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PMID: 20563332
PDF is available here.
PDF is available here.
Abstract
We will describe the current state of the art in nucleic acid diagnostics, the use of nucleic acid-based diagnostics in clinical practice and the emerging technologies in the field. Finally, we will describe future trends and expected advances in the field....
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PMID: 20583889
PDF is available here.
PDF is available here.
Journal of Fluorescence (20)4 2010
Abstract
It is found that silver nanoparticles (AgNPs) can further enhance the fluorescence intensity of curcumin (CU)-cetyltrimethylammonium bromide (CTAB)-nucleic acids and improve its anti-photobleaching activity. Under optimum conditions, the enhanced fluorescence intensity is proportion to the concentra...
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PMID: 20204682
PDF is available here.
PDF is available here.
Abstract
Enterohemorrhagic E. coil (EHEC) serotype O157:H7 is one of the major pathogens, responsible for the severe disease outbreaks. EHEC causes diseases in humans through production of shiga-like toxin leading to bloody diarrhea. The toxin is encoded by stx2 gene in E. coli. The current methodology for d...
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PMID: 21128394
PDF is available here.
PDF is available here.
Nanomedicine (5)5 2010
Abstract
Prenatal Diagnosis (30)7 2010
Abstract
Biochemistry (Washington) (49)23 2010
Abstract
We monitored structural changes occurring in HCV core protein and loop IIId upon formation of nucleocapsid-like particles (NLPs). The protein secondary structure of these particles involves beta-sheet enrichment in relation to its protein monomer. The phosphodiester backbone vibrations of loop IIId...
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PMID: 20469947
PDF is available here.
PDF is available here.
Abstract
The discovery of circulating nucleic acids in the 1940s opened up new possibilities for the non-invasive detection, monitoring and screening of various human disorders. Several tumour markers that enable early cancer detection or tumour behaviour prediction have been detected in the plasma of cancer...
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PMID: 20186496
PDF is available here.
PDF is available here.
Chemical Reviews (110)5 2010
Abstract
Abstract
I highlight several outlandish features of the human genome that defy notions of ID by a caring cognitive agent. These range from de novo mutational glitches that collectively kill or maim countless individuals (including embryos and fetuses) to pervasive architectural flaws (including pseudogenes,...
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PMID: 20445101
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)19 2010
Abstract
We show that prions causing transmissible spongiform encephalopathy in wild-type hamsters can be generated solely from highly purified, bacterially expressed recombinant hamster prion protein without any mammalian or synthetic cofactors (other than buffer salts and detergent). These findings provide...
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PMID: 20304915
PDF is available here.
PDF is available here.
Abstract
We describe the recent advances that have been made by research into the role of TLR biology in host defense and disease....
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PMID: 20404851
PDF is available here.
PDF is available here.
Renal Failure (32)4 2010
Abstract
We hypothesized that the available circulating nucleic acids, acting as DAMPs, may modulate immunoinflammatory reaction in CRF. Patients with the different stages of chronic kidney disease, kidney transplantation, and patients on dialysis were included in the study. Obtained results about higher con...
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PMID: 20446789
PDF is available here.
PDF is available here.
Clinica Chimica Acta (411)9-10 2010
Abstract
BACKGROUND: Cell-free DNA is observed to be more in exudative pleural effusions. Based on this fact development of a clinical chemistry test for classification of pleural effusion will require DNA extraction followed by PCR amplification and electrophoresis. These procedures may not be cost effectiv...
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PMID: 20138858
PDF is available here.
PDF is available here.
Abstract
We show that the theoretical limit on genomic information content may increase substantially as a consequence of dramatically slowed polymerization after mismatches. As a result of postmismatch stalling, accurate copies of a template tend to be completed more rapidly than mutant copies and the accur...
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PMID: 20359213
PDF is available here.
PDF is available here.
Abstract
We tested the hybrid monolith on a variety of previously developed pAE capabilities including control of ON selectivity, resolution of derivatized ONs, the ability to resolve RNA ONs harboring aberrant linkages at different positions in a single sequence and separation of phosphorothioate diastereoi...
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PMID: 20226746
PDF is available here.
PDF is available here.
Biophysical Journal (98)8 2010
Abstract
We report a new computational approach to the prediction of RNA secondary structure folding kinetics. In this approach, each elementary kinetic step is represented as the transformation between two secondary structures that differ by a helix. Based on the free energy landscape analysis, we identify...
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PMID: 20409482
PDF is available here.
PDF is available here.
Neurosurgery (66)4 2010
Abstract
Abstract
The polymerase chain reaction (PCR) has matured from a labour- and time-intensive, low throughput qualitative gel-based technique to an easily automated, rapid, high throughput quantitative technology. Real-time quantitative PCR (qPCR) has become the benchmark technology for the detection and quanti...
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PMID: 20025972
PDF is available here.
PDF is available here.
Abstract
Circulating nucleic acids are present in the blood of humans and other vertebrates. During the last 10 years researchers actively studied cell-free nucleic acids present in plasma or serum with great expectations of their use as potential biomarkers for cancer and other pathologic conditions. In the...
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PMID: 20146940
PDF is available here.
PDF is available here.
Abstract
We review the entire workflow from the planning and preparation phase, over the actual real-time PCR cycling experiments to data-analysis and reporting steps. This process can be captured with the appropriate acronym PCR: plan/prepare, cycle and report. The key message is that quality assurance and...
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PMID: 19969088
PDF is available here.
PDF is available here.
Proteomics (10)7 2010
Abstract
We report the identification of 2315 B. japonicum strain USDA110 proteins (27.8% of the theoretical proteome) that are expressed 21 days post infection in symbiosis with soybean cultivated in growth chambers, substantially expanding the previously known symbiosis proteome. Integration of transcripto...
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PMID: 20104621
PDF is available here.
PDF is available here.
Medical Science Monitor (16)4 2010
Abstract
Molecular analysis of fetal DNA present in the maternal circulation allows noninvasive, early, and precise determination of fetal genetic status in prenatal diagnostics. The most common clinical applications, i.e. prenatal gender determination and fetal RhD genotyping, are possible already in the fi...
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PMID: 20357733
PDF is available here.
PDF is available here.
Abstract
Methods in Molecular Biology (630)630 2010
Abstract
We report a detailed procedure for the exact quantification of minute amounts of nucleic acids by competitive PCR. This technique entails the co-amplification of a target DNA or cDNA in a biological sample together with a known quantity of a target-specific standard, the competitor, which is added e...
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PMID: 20301001
PDF is available here.
PDF is available here.
Abstract
We show here that CvfB binds RNA. The crystal structure of the CvfB ortholog from Streptococcus pneumoniae at 1.4 A resolution reveals a unique RNA binding protein that is formed from a concatenation of well-known structural modules that bind nucleic acids: three consecutive S1 RNA binding domains a...
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PMID: 20399190
PDF is available here.
PDF is available here.
Abstract
Since the association of circulating DNA level changes with tumor growth was discovered many attempts have been made to develop the sensitive and robust blood-based tests for early tumor diagnostics. Both genomic as well as mitochondrial DNA quantification in the circulation have been extensively ev...
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PMID: 20196731
PDF is available here.
PDF is available here.
Abstract
We have characterized a cell permeant, far red fluorescent nucleic acid-binding dye, SYTO 61, that can be used to distinguish between uninfected and infected erythrocytes in a flow cytometric format. The spectral properties of SYTO 61 make it suitable for use in combination with the fluorescent reac...
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PMID: 20091670
PDF is available here.
PDF is available here.
Abstract
We have explored the use of SYTO 13, a member of the class of SYTO dyes, for particle detection. SYTO 13 is cell permeable and has a high fluorescent yield when bound to DNA or RNA. In this study, we compared detection of MPs using either light scatter or SYTO 13 staining, testing the hypothesis tha...
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PMID: 20104574
PDF is available here.
PDF is available here.
European Journal of Pharmacology (628)1-3 2010
Abstract
Current treatment options for acute pancreatitis are supportive and symptomatic. Due to lack of agents targeting the underlying pathophysiology a large amount of experimental work is going on to identify novel therapeutic agents. The present study was carried out to explore if melatonin can modulate...
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PMID: 19958759
PDF is available here.
PDF is available here.
Fa yi xue za zhi (26)1 2010
Abstract
There were not obvious changes for the main FTIR absorbance peaks. But the different FTIR absorbance at the wave-number (cm(-1)) indicated the three types: increase, decrease, stable. The various absorbance ratios also demonstrated the similar changes. CONCLUSION: FTIR spectroscopy may be potentiall...
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PMID: 20232733
PDF is available here.
PDF is available here.
Abstract
The origins of repeated hematoxylin shortages are outlined. Lack of integration in the hematoxylin trade exacerbates the problems inherent in using a natural product. Separate corporations are engaged in tree growth and harvesting, dye extraction, processing of extracts to yield hematoxylin, and for...
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PMID: 19562570
PDF is available here.
PDF is available here.
Abstract
This review examines acyclic nucleoside analogs as therapeutic agents, potential progenitor candidates to RNA, and novel building blocks for nucleic-acid nanotechnology. Together, these areas of research provide new insights into the structural and functional properties of nucleic ac...
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PMID: 20151377
PDF is available here.
PDF is available here.
Abstract
Recent single molecule studies have made a significant contribution to the understanding of the molecular mechanism involved in the movement of motor proteins which process DNA and RNA. Measurement of stepsize in two disparate motors, NS3 helicase and ribosome both revealed 3-bp steps, which consist...
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PMID: 20061135
PDF is available here.
PDF is available here.
Abstract
Abstract
We could successfully eliminate the dangerous phenol/chloroform extraction steps from the isolation procedure. Both spectrophotometric (A(260)/A(280) and A(260)/A(230) ratios) and agarose electrophoresis analysis of isolated nucleic acids (DNA and RNA) indicated good results. DNA with the average yi...
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PMID: 19578976
PDF is available here.
PDF is available here.
Journal of Bacteriology (192)4 2010
Abstract
I and II, and nucleic acids, as being discriminatory. Molecular analyses confirmed a dramatic drop in cellular yields of both the phospholipid fatty acids and their precursor molecules at high concentrations of silver, suggesting that the structural integrity of the cellular membrane was compromised...
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PMID: 20008067
PDF is available here.
PDF is available here.
Bioscience Reports (30)1 2010
Abstract
I band and shifting of the position of this band to higher frequency values in treated muscle indicates structural changes in proteins. Detailed secondary structure analysis of the amide I band revealed a significant increase in antiparallel and aggregated beta-sheet, random coil structure and a sig...
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PMID: 19228123
PDF is available here.
PDF is available here.
Abstract
A misfolded isoform of the prion protein (PrP) is the essential component of the prion diseases' agent. The prion concept has progressively gained acceptance, in a large part thanks to the realization that it played a role not only in the transmissible spongiform encephalopathies, but also in the no...
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PMID: 20036833
PDF is available here.
PDF is available here.
Abstract
We will explore the interaction of PrP with some of its most intriguing partners, such as sulfated glycans and nucleic acids. These molecules seem to play a dual role in prion biology and could be fundamental to explaining how prion diseases arise, as well as in the development of effective therapeu...
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PMID: 20036811
PDF is available here.
PDF is available here.
Abstract
Abstract
Surface plasmon resonance (SPR) technology with biosensor surfaces has become a widely-used tool for the study of nucleic acid interactions without any labeling requirements. The method provides simultaneous kinetic and equilibrium characterization of the interactions of biomolecules as well as smal...
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PMID: 19997874
PDF is available here.
PDF is available here.
Abstract
Rapid increase in drug-resistant influenza virus isolates, and pandemic threat posed by highly pathogenic avian influenza A and swine flu viruses provide clear and compelling reasons for fast tracking development of novel antiviral drugs. Nucleic acid-based drugs represent a promisin...
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PMID: 20036985
PDF is available here.
PDF is available here.
Abstract
Biomolecular assays are continually being developed that use progressively smaller amounts of material, often precluding the use of conventional cuvette-based instruments for nucleic acid quantitation for those that can perform microvolume quantitation. The NanoDrop microvolume sample retention syst...
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PMID: 21189466
PDF is available here.
PDF is available here.
Physical Biology (7)4 2010
Abstract
We perform Brownian dynamics on a coarse-grained model that describes the dynamics of icosahedral capsid assembly around a flexible polymer. We identify several mechanisms by which the polymer plays an active role in its encapsulation, including cooperative polymer-protein motions. These mechanisms...
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PMID: 21149971
PDF is available here.
PDF is available here.
Abstract
Nucleic acids are attractive materials for creating functional molecules that have applications as catalysts, specific binders, and molecular switches. Nucleic acids having such functions can be obtained by random screening, typically using in vitro selection methods. These methods have helped explo...
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PMID: 20714306
PDF is available here.
PDF is available here.
Abstract
The hammerhead ribozyme was first considered as a metalloenzyme despite persistent inconsistencies between structural and functional data. In the last decade, metal ions were confirmed as catalysts in self-splicing ribozymes but displaced by nucleobases in self-cleaving ribozymes. However, a model o...
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PMID: 20714304
PDF is available here.
PDF is available here.
Molecular Cancer (9) 2010
Abstract
Our results indicate that men who test positive following CTC enrichment also exhibit higher detectable levels of non-cellular, circulating prostate-specific mRNAs....
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PMID: 20598135
PDF is available here.
PDF is available here.
Abstract
We evaluated two novel products for room temperature DNA storage: Biomatrica's DNA SampleMatrix technology and GenVault's GenTegra DNA technology. We compared the integrity and quality of DNA stored using these products against DNA stored in a -20 C freezer by performing downstream testing with shor...
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PMID: 19801719
PDF is available here.
PDF is available here.