Welcome to the Pubget Medical Subject Headings (MeSH) browser.
Click on a topic or subtopic below to explore related papers. You'll find the most recent paper about the current MeSH term along with other related papers below.
DNA, Single-Stranded (1)
Articles on DNA, Single-Stranded
SMARCAL1 catalyzes fork regression and Holliday junction migration to maintain genome stability during DNA replication.
We investigated its mechanism of action. We found that SMARCAL1 travels with elongating replication forks, and its absence leads to MUS81-dependent double-strand break formation. Binding to specific nucleic acid substrates activates SMARCAL1 activity in a reaction that requires its HARP2 (Hep-A-rela...
Trinucleotide repeat DNA alters structure to minimize the thermodynamic impact of 8-oxo-7,8-dihydroguanine.
We show that TNR DNA adapts its structure to accommodate 8-oxoG. Using chemical probe analysis, we find that CAG repeats composing the stem-loop arm of a three-way junction alter the population of structures in response to 8-oxoG by positioning the lesion at or near the loop. Furthermore, we find th...
DNA binding activity of Helicobacter pylori DnaB helicase: the role of the N-terminal domain in modulating DNA binding activities.
We have analysed the DNA binding activity of Helicobacter pylori (Hp) replicative helicase, DnaB. The results indicate that while the C-terminal region is important for its DNA binding activity, the N-terminus appears to dampen the protein's affinity for DNA. The masking activity of the N-terminus d...
Polymerization and nucleic acid-binding properties of human L1 ORF1 protein.
We purified human L1 ORF1p expressed in insect cells and made two findings that significantly advance our knowledge of the protein. First, in the absence of nucleic acids, the protein polymerizes under the very conditions (0.05 M NaCl) that are optimal for high (∼1 nM)-affinity nucleic acid bi...
DNA-induced dimerization of the single-stranded DNA binding telomeric protein Pot1 from Schizosaccharomyces pombe.
We found an unanticipated ssDNA-binding mode in which two SpPot1 molecules bind an oligonucleotide containing two telomeric repeats. DNA binding seems to be achieved via binding of the most N-terminal OB domain of each monomer to each telomeric repeat. The SpPot1 dimer may have evolved to accommodat...
A conserved lysine residue of plant Whirly proteins is necessary for higher order protein assembly and protection against DNA damage.
We add a new layer of complexity to this model by showing through atomic force microscopy that tetramers of the potato Whirly protein WHY2 further assemble into hexamers of tetramers, or 24-mers, upon binding long DNA molecules. This process depends on tetramer-tetramer interactions mediated by K67,...
OIP30, a RuvB-like DNA helicase 2, is a potential substrate for the pollen-predominant OsCPK25/26 in rice.
We isolated OsCPK25/26-interacting protein 30 (OIP30), which is also predominantly expressed in pollen. OIP30 encodes a RuvB-like DNA helicase 2 (RuvBL2) that is well conserved in eukaryotic species from yeast to human. Yeast and Drosophila defective in RuvBL2 are non-viable. The interaction between...
Sorting the unique chirality, right handed single wall carbon nanotubes via the dye modified ssDNA.
The unique (n, m) SWCNTs have both left- and right-handed helicity and they are enantiomers, and unique chiral SWCNTs with single helicity haven't achieved yet. In our studies the aromatic fluorescence molecule, (R+) 5'-Hexachloro-Fluorescein Phosphoramidite (HEX), was linked to DNA,...
DNA-templated ensemble for label-free and real-time fluorescence turn-on detection of enzymatic/oxidative cleavage of single-stranded DNA.
We have demonstrated a DNA-templated small molecule ensemble that allows the label-free fluorescence turn-on detection of enzymatic or oxidative cleavage of single-stranded DNA with high sensitivity and selectivity.
Synthesis of β-pyrrolic-modified porphyrins and their incorporation into DNA.
A synthetic methodology for the synthesis of various β-pyrrolic-functionalised porphyrins and their covalent attachment to 2'-deoxyuridine and DNA is described. Palladium(0)-catalysed Sonogashira and copper(I)-catalysed Huisgen 1,3-dipolar cycloaddition reactions were used to insert...