Abstract
Kendall's tau-b and Chi-square test were used to assess the correlation between the BANA test results and CPITN scores. Results indicated sensitivity (92.86%), specificity (80%) and agreement (91.25%); indicating the validity of CPITN in assessing anaerobic infection. CONCLUSION: There was a signifi...
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PMID: 20139564
PDF is available here.
Abstract
The purpose of the present investigation was to evaluate the effects of sodium lauryl sulphate (SLS), present in a commercial dentifrice, on the formation of volatile sulphur compounds (VSC) and tongue coating in a panel of periodontally healthy subjects. A two-step blinded, crossover, randomized st...
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PMID: 19055226
PDF is available here.
Abstract
I collagen [ICTP]) analyses, respectively, at baseline, 2 weeks, and 1, 3, and 6 months.
Surgical treatment of moderate (PD = 4 to 6 mm) and deep (PD > 6 mm) pockets significantly improved clinical parameters of treated and untreated teeth (CAL gain, PD reduction, and reduction of BOP). The addition...
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PMID: 17915999
PDF is available here.
Abstract
In approximately 80-90% of all cases, halitosis is caused by oral conditions, defined as oral malodour. Oral malodour results from tongue coating, periodontal disease, peri-implant disease, deep carious lesions, exposed necrotic tooth pulps, pericoronitis, mucosal ulcerations, healing (mucosal) woun...
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PMID: 17555859
PDF is available here.
Abstract
Persons with coronary heart disease, particularly associated with an acute coronary syndrome, have anaerobic bacterial dental flora similar to individuals seeking periodontal dental care, and unlike periodontally healthy individuals....
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PMID: 17301602
PDF is available here.
Abstract
Initially, intermediate sites (4-6 mm) in the test group showed less plaque accumulation, gingival bleeding, bleeding on probing and a greater reduction in attachment level and probing depth (PD) at 63 days after treatment. The initially deep sites (>6 mm) in the CHX group also showed a better reduc...
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PMID: 16965522
PDF is available here.
Abstract
Periodontal disease can be diagnosed chairside by the presence of white blood cells in plaque samples, a finding that reflects the inflammatory nature of the disease process. This approach would reduce the misclassification of subjects as having periodontal disease (130 patients in the present study...
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PMID: 16945036
PDF is available here.
Abstract
Gingivitis is the first manifestation of periodontal disease, and is characterized by painless and slow evolution. Early diagnosis and intervention must be done to avoid the possibility of precocious periodontitis during the childhood or teenage years. The enzymatic BANA test (N-benzoyl-DL-arginine-...
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PMID: 16865996
PDF is available here.
Abstract
We used a chairside assay for the N-benzoyl-DL-arginine-2-naphthylamide (BANA)-sensitive enzyme found in Porphyromonas gingivalis, Treponema denticola, and Tannerella forsythensis, to determine the prevalence of these BANA-positive species in young children and their caregivers. We predicted that if...
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PMID: 16434739
PDF is available here.
Abstract
The scores of PI, GI, BI and sample that tested positive for halitosis linked toxins and with the halitosis grading were not significant. The presence of tongue coating and the halitosis grading and toxin levels were significant. BANA has shown to be non contributory due to technical problems. Anaer...
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PMID: 16900888
PDF is available here.
Abstract
We assessed the gingival health of all subjects using the gingival inflammation (M-PMA) index and periodontal probing depth (PD). Children were divided into three age categories: <5 years (AI), 5 to <10 years (AII), and 10 to <17 years (AIII). Supragingival plaque was measured using the Oral Hygiene...
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PMID: 16703929
PDF is available here.
Abstract
Individuals with repaired cleft lip and/or palate can have the same VSC levels as subjects without clefts....
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PMID: 16301148
PDF is available here.
Abstract
IL-6 levels were significantly higher in participants with more-extensive periodontal disease than in other participants. Periodontal disease extent was significantly associated with higher TNF-alpha plasma levels, controlling for established risk factors for elevated TNF-alpha levels. Participants...
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PMID: 16137283
PDF is available here.
Abstract
Both groups experienced comparable levels of PD reduction following AFS (test: -1.57 +/- 0.34; control: -1.50 +/- 0.21). Changes in mean CAL were more favorable in Vit-B supplemented subjects (test: +0.41 +/- 0.12; control: -0.52 +/- 0.23; P = 0.024). Stratified data demonstrated significantly bette...
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PMID: 16018750
PDF is available here.
Abstract
The utilization of AZM in combination with SRP improves the efficacy of non-surgical periodontal therapy in reducing probing depth and improving attachment levels in smokers with moderate to advanced attachment loss....
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PMID: 15857078
PDF is available here.
Abstract
Even though the presence of BANA positive activity was similar among DS, MR and MH subjects, halitosis seemed to be lower in the DS group....
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PMID: 15752108
PDF is available here.
Abstract
This article, the fourth in a series, examines nonradiographic procedures and devices such as standard clinical and visual examination, apex locators, vitality testers and colour shade guides in light of the tools described in the first 2 articles in the series. A variety of nonradiographic indices...
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PMID: 15245689
PDF is available here.
Abstract
According to the result of AST analysis, 45 sites were AST positive and 50 were AST negative in the diabetic group and 36 sites were AST positive and 38 were AST negative in the control group. There was a significant correlation between BANA hydrolysis and PPD in both diabetic and control groups, bu...
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PMID: 15002891
PDF is available here.
Abstract
The objective of this study was to assess the association between the levels of enzyme aspartate aminotransferase (AST) in gingival crevicular fluid (GCF) with the BANA hydrolysis microbiological test (Perioscan) and clinical periodontal diagnostic measurements, such as bleeding on probing, plaque i...
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PMID: 12816362
PDF is available here.
Abstract
The benzoyl-DL arginine-naphthylamide (BANA) test for detecting digesting peptidase and polymerase chain reaction (PCR) measurement of pathogenic bacteria were performed in 15 patients with periodontal disease. The number of Porphyromonas gingivalis, Bacteroides forsythus and Treponema denticola, wh...
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PMID: 12854883
PDF is available here.
Abstract
The scores of PI, GI, subgingival samples that tested positive for BANA hydrolyzing species, organoleptic ratings, and VSC values were significantly higher in the subjects with PD > 3.0 mm (P < 0.01, Mann-Whitney U test). There was a correlation between BANA hydrolysis by subgingival plaque bacteria...
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PMID: 12479639
PDF is available here.
Abstract
Both groups presented significant improvements in all parameters analyzed over the study period. There were no statistically significant differences between the two groups for any parameter analyzed after 9 months, except for BOP, which was significantly reduced in the control group. The mean reduct...
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PMID: 12445218
PDF is available here.
Abstract
Our findings suggest that immunosuppressed patients showed significantly less inflammation and fewer putative anaerobic pathogens using the BANA test, and that patients undergoing therapy with cyclosporin-A have a higher frequency of sites with gingival hyperplasia when compared with patients medica...
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PMID: 12240891
PDF is available here.
Abstract
We identified the gene, designated opdB, in an apparently noncoding region of the T. denticola genome unannotated contigs (11/2000; http://www.tigr.org). The opdB gene begins with a TTG start codon and encodes a 685-residue peptide with high homology to the oligopeptidase B family in prokaryotes and...
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PMID: 11553560
PDF is available here.
Abstract
The average proteolytic activity at the dentin substrates (OD) at TO and 2, 4, 8 and 12 wks thereafter were 0.794+/-0.089, 0.741+/-0.071, 0.676+/-0.087, 0.600+/-0.094, and 0.508+/-0.108 respectively. The chlorhexidine varnish mediated a significant inhibition of the proteolytic activity present in d...
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PMID: 11572285
PDF is available here.
Abstract
We show a significant correlation between an index widely used in clinical practice, the Gingival Index of Löe (1967), based on the presence or absence of bleeding on probing, and the methodology of the BANA test for the detection of the specific enzymatic activity of microorganisms involved in per...
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PMID: 11210245
PDF is available here.
Abstract
This study evaluates the effect of subgingival irrigation with a 1% chlorhexidine collagen gel in periodontal pockets as an adjunct procedure to scaling and root planing (SRP). Thirty-seven sites with probing depth (PD) of 5-7 mm and BANA positive in 6 patients with chronic periodontal disease were...
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PMID: 11696921
PDF is available here.
Abstract
The pS level of the representative sites with low to moderate bone loss demonstrated a modest association with oral malodor. Oral malodor in periodontal patients was primarily associated with tongue coating and gingival inflammation....
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PMID: 11210077
PDF is available here.
Abstract
The pS level may be a potential indicator for detecting severity of periodontal disease and identifying bacteria that are capable of hydrolyzing BANA....
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PMID: 11210076
PDF is available here.
Abstract
A random sample of sixty-two 11-15-year-old adolescents from 17 different locations in Guatemala were selected for this study. Pocket depth, Plaque Index, and bleeding upon probing were recorded from 6 randomly selected sites in each subject (a total of 372 sites). Subgingival plaque samples were su...
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PMID: 11210274
PDF is available here.
Abstract
The purpose of the present investigation was to determine whether subjects institutionalized with mental retardation have a relationship between periodontal clinical parameters and the presence of the BANA-positive periodontal pathogens Porphyromonas gingivalls, Treponema denticola, and Bacteroides...
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PMID: 11203898
PDF is available here.
Abstract
Smoking has been identified as a risk factor for development of periodontal disease and a strong indicator for treatment failure in periodontal patients. This study examined 172 patients categorized as current smokers (n=55), previous smokers (n=38) or individuals that had never smoked (n=79). A tot...
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PMID: 10599910
PDF is available here.
Abstract
We purified and characterized a protease from Actinobacillus actinomycetemcomitans. The protease was isolated from the culture supernatant by sonication in phosphate-buffered 3-[(3 cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS). The protease was purified by acetone precipitation, follow...
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PMID: 10232464
PDF is available here.
Abstract
In the present investigation the ability of subgingival plaque to hydrolyze BANA (Perioscan) was correlated with CPITN scores. Among 281 sites investigated, 136 had a CPITN equal to 2 with a highly significant positive BANA value (107 sites). A CPITN equal to 3 was also significantly BANA positive (...
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PMID: 10863395
PDF is available here.
Abstract
We have found that the cell lysate from cultured human normal keratinocytes from foreskin (HFKs) hydrolyzed alpha-N-benzoyl-DL-arginine beta-naphthylamide (BANA), and the BANA hydrolysis occurred most under conditions of 37 degrees C and pH 6.0. This activity was strongly inhibited by leupeptin, whi...
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PMID: 10363162
PDF is available here.
Abstract
The aim of this cross-sectional study was to investigate the clinical application of chairside tests for gingival crevicular fluid (GCF) aspartate aminotransferase (AST) levels and plaque BANA hydrolysis activity with the presence of the periodontal pathogens Porphyromonas gingivalis and Actinobacil...
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PMID: 9565286
PDF is available here.
Abstract
The potential of bacterial markers for the detection of periodontal disease activity is discussed. Chronic periodontitis has been associated with subgingival bacteria. Techniques are available to detect and distinguish different bacteria. In subgingival plaque, bacterial proteases can be detected. C...
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PMID: 9549909
PDF is available here.
Abstract
Several recent studies have shown a link between dental disease and coronary heart disease. The authors studied 320 U.S. veterans in a convenience sample to assess the relationship between oral health and systemic diseases among older people. They present cross-sectional data confirming that a stati...
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PMID: 9529805
PDF is available here.
Abstract
The effects of several protease substrates or protease inhibitors on neuromuscular transmission in the isolated mouse phrenic nerve-diaphragm were studied. N-Carbobenzoxy-Gly-Gly-Arg-beta-naphthylamide (Z-GGR-N) but none of the other agents inhibited the nerve-evoked muscle contractions. By means of...
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PMID: 9551714
PDF is available here.
Abstract
We consider the BANA test to be of practical applicability in periodontal clinical practice and to represent an important auxiliary diagnostic tool for patients with adult periodontitis....
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PMID: 10219119
PDF is available here.
Abstract
We have used a chairside test for BANA hydrolysis to diagnose an anaerobic periodontal infection in patients with advanced forms of clinical disease using a 15-min/55 degrees C incubation protocol. However, the BANA test performance is dependent upon the length and temperature of incubation. In the...
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PMID: 9350555
PDF is available here.
Abstract
Ninety-two subjects were tested before orthodontic mechanotherapy for the presence of three putative periodontopathogens-Porphyromonas gingivalis, Treponema denticola, and Bacteroides forsythus-with the BANA test (PerioScan Oral-B). The sample ranged from 9 to 16 years, with a mean age of 13.1 years...
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PMID: 9267226
PDF is available here.
Abstract
The tissue kallikrein inhibitors reported in the present work were derived by selectively replacing residues in Nalpha-substituted arginine- or phenylalanine-pNA (where pNA is p-nitroanilide), and in peptide substrates for these enzymes. Phenylacetyl-Arg-pNA was found to be an efficient inhibitor of...
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PMID: 9173877
PDF is available here.
Abstract
We have taken this 0.8 cut point to look at specific clinical decisions made by our examiners after the patients had received scaling and root planing plus 2 weeks unsupervised usage of systemic antimicrobials. The clinicians' decision was taken as the primary reference standard. The model's estimat...
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PMID: 9083901
PDF is available here.
Abstract
Perioscan requires a plaque sample to detect the presence of enzymes capable of degrading N-benzoyl-DL-arginine-2-naphthylamide (BANA) from relatively few anaerobic periodontal pathogens. Periocheck assays the presence of neutral proteases in crevicular fluid. The aim of this study was to compare th...
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PMID: 9062857
PDF is available here.
Abstract
The periodontal condition of 30 type II non-insulin-dependent diabetic patients (NIDDM) was evaluated and compared to that of 30 non-diabetic patients (age range, 30-77 years). Glycosylated hemoglobin and fasting glucose tests were used to measure the metabolic control of the patients, and the BANA...
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PMID: 9485634
PDF is available here.
Abstract
Counts of cultivable Porphyromonas gingivalis, assays of microbial proteases and the concentration in gingival crevicular fluid of proteoglycan metabolites were investigated at periodontitis and gingivitis sites in 16 patients with chronic adult periodontitis before and after treatment. Two periodon...
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PMID: 9134120
PDF is available here.
Abstract
The mutual effects of P. gingivalis and several cystatin species has been investigated. After incubation with P. gingivalis culture supernatant, cystatin S, cystatin C and chicken cystatin were truncated from a 14 kDa protein into a polypeptide of approximately 13 kDa. Amino acid sequence analysis o...
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PMID: 8997496
PDF is available here.
Abstract
We have previously shown that protoporphyrin IX and inorganic iron can replace the hemin requirement, suggesting that the hemin requirement of this microorganism is actually a porphyrin requirement. We examined the effect of protoporphyrin IX limitation to P. gingivalis strain A7A1-28 in the presenc...
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PMID: 8935059
PDF is available here.
Abstract
These results suggest that AcH does not react with the catalytic site of thrombin, which is protected in the prothrombin molecule. However, it does react with susceptible sites on the prothrombin surface which remain alkylated during extensive dialysis to remove excess AcH and subsequent activation...
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PMID: 8888937
PDF is available here.
Abstract
Cathepsin B (EC 3.4.22.1) was purified from buffalo liver. The enzyme activity against alpha-benzoyl-DL-arginine-naphthylamine (BANA) was substantially reduced by heat (above 37 degrees C) and by nondenaturing concentrations of urea (3 M) and guanidine hydrochloride (1 M). Cathepsin B was significan...
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PMID: 8838593
PDF is available here.
Abstract
Research suggests that the tongue plays an important role in the production of oral malodor. To investigate the role of tongue surface characteristics and oral bacteria in halitosis development, the authors tested associations between odor measurements, volatile sulfur compound levels, periodontal p...
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PMID: 7594010
PDF is available here.
Abstract
Bad breath (halitosis, oral malodor) is a common condition, usually the result of microbial putrefaction within the oral cavity. Often, people suffering from bad breath remain unaware of it, whereas others remain convinced that they suffer from foul oral malodor, although there is no evidence for su...
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PMID: 7560419
PDF is available here.
Abstract
In this study, the adjunctive effects of subgingival irrigation by 1.64% stannous fluoride after subgingival scaling were evaluated in 10 patients with moderate periodontal disease. Three pockets related to single rooted teeth, each in a separate quadrant were monitored in each patient. After proper...
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PMID: 9497666
PDF is available here.
Abstract
Further longitudinal studies are suggested to investigate the role of the TLPase in the response to treatment of chronic adult periodontitis patients....
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PMID: 7553388
PDF is available here.
Abstract
We compared proteinase-activity in serum of 20 untreated HNSCC patients with that of 20 non-cancer individuals. When N-benzoyl-DL-arginine-beta-naphtylamide (BANA) was used as the substrate, proteinase-activity seemed higher among patients, but this difference disappeared after adjustment for alcoho...
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PMID: 8669860
PDF is available here.
Abstract
Considerable evidence exists suggesting that periodontal disease is due to the overgrowth of a finite number of specific bacteria such as Porphyromonas gingivalis, Actinobacillus actinomycetemcomitans, Treponema denticola, Bacteroides forsythus, and Prevotella intermedia, among others. Three of thes...
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PMID: 7929977
PDF is available here.
Abstract
We carried out stepwise forward multiple regression. Results showed that VSC and cadaverine both factor significantly in explaining each of the odor judge measurements, with multiple r values ranging from 0.545 (p = 0.0002) to 0.604 (p < 0.0001). The results suggest that cadaverine levels are associ...
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PMID: 8046106
PDF is available here.