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Guanidine (0):
Recent article
Guanidine
Abstract
We examined the conformational transitions in a multimeric and high molecular weight class II α-mannosidase from Canavalia ensiformis (Jack Bean) (Jbα-man) utilizing intrinsic fluorescence, solute quenching, hydrophobic dye binding, size exclusion chromatography and circular dichroism (CD) spectro...
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PMID: 21087596
PDF is available here.
PDF is available here.
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Organic Letters (12)21 2010
Abstract
Bioassay-guided fractionation of the sub-Arctic ascidian Synoicum pulmonaria collected off the Norwegian coast led to the isolation of a novel family of brominated guanidinium oxazolidinones named synoxazolidinones A and B (1 and 2). The backbone of the compounds contains a 4-oxazoli...
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PMID: 20883040
PDF is available here.
PDF is available here.
Abstract
We studied cation selectivity by outward-facing sites (high K(+) affinity) of Na/K pumps expressed in Xenopus oocytes, under voltage clamp. Guanidinium(+), methylguanidinium(+), and aminoguanidinium(+) produced two phenomena possibly reflecting actions at site III: (i) voltage-dependent inhibition (...
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PMID: 20937860
PDF is available here.
PDF is available here.
Abstract
The N-(pyrrolidin-2-ethyl) glycine-based PNA (pet-PNA) backbone, with 4-amino or 4-guanidino-functionalized pyrrolidine ring, confers constrained conformational flexibility on aegPNA. The oligomers bind to the target DNA and RNA sequences with increased sequence specificity and antiparallel versus p...
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PMID: 20539879
PDF is available here.
PDF is available here.
Abstract
We analysed the secondary structure and structural organization of a purified 65kDa N-domain ACE (nACE) from Wistar rat mesangial cells, a 90 kDa nACE from spontaneously hypertensive rats and a 130 kDa somatic ACE. The C-terminal alignment of the 65 kDa nACE with rat ACE revealed that the former was...
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PMID: 20435057
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)29 2010
Abstract
We have developed two nanoparticle formulations, cationic liposome-polycation-DNA (LPD) and anionic liposome-polycation-DNA (LPD-II), for systemic co-delivery of doxorubicin (Dox) and a therapeutic small interfering RNA (siRNA) to multiple drug resistance (MDR) tumors. In this study, we have provide...
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PMID: 20460382
PDF is available here.
PDF is available here.
Journal of Fluorescence (20)4 2010
Abstract
The conformational transitions in an oligomeric and high molecular weight class II alpha-mannosidase from Aspergillus fischeri were examined using fluorescence and CD spectroscopy under chemical, thermal and acid denaturing conditions. The enzyme lost the activity first and then the overall folded c...
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PMID: 20204683
PDF is available here.
PDF is available here.
Abstract
This method reproducibly detected as low as 4-6 bacilli. Of 102 clinical samples, 84 were human immunodeficiency virus (HIV) negative, while 18 were HIV-positive. Among the HIV-negative individuals, 58.3% were TB-positive using PCR, while respectively 47.6% and 45.2% were sputum- and culture-positiv...
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PMID: 20550767
PDF is available here.
PDF is available here.
Abstract
Trp(Nps)-Lys-NH(2) derivatives, bearing alkyl or guanidine groups either at the N-terminus or on the Lys side-chain or at both positions were conveniently prepared on solid-phase and evaluated as TRPV1 channel antagonists.
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PMID: 20657400
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1804)7 2010
Abstract
The stability of bovine lactoperoxidase to denaturation by guanidinium-HCl, urea, or high temperature was examined by differential scanning calorimetry (DSC) and tryptophan fluorescence. The calorimetric scans were observed to be dependent on the heating scan rate, indicating that lactoperoxidase st...
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PMID: 20298816
PDF is available here.
PDF is available here.
Abstract
We investigated the action of the quaternary ammonium salt (QAS) called IM (N-(dodecyloxycarboxymethyl)-N,N,N-trimethyl ammonium chloride) on Saccharomyces cerevisiae yeast cells. Changes in the yeast cell ultrastructure were confirmed by electron microscopy. We treated resistant mutant cells with Q...
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PMID: 20140761
PDF is available here.
PDF is available here.
Abstract
A new chiral bicyclic guanidine-catalyzed direct catalytic aldol reaction of 5H-oxazol-4-ones with aldehydes has been developed. The present aldol reaction proceeds smoothly with high enantioselectivity using bicyclic guanidines bearing a hydroxy group at the appropriate position, and various combin...
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PMID: 20408533
PDF is available here.
PDF is available here.
Human Gene Therapy (21)5 2010
Abstract
The delivery of genes or viruses via liposomes is a common approach used to enhance delivery efficiency. In the current study, to enhance delivery efficiency, proteoliposomes (PLs) containing adenovirus (Ad) were synthesized with dimyristoylphosphatidylcholine (DMPC), cholesterol, and apolipoprotein...
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PMID: 20038214
PDF is available here.
PDF is available here.
Abstract
We observed that at higher pH, BSF exists in alkaline unfolded state. Our results provided evidence that correlates simultaneous formation of secondary structure followed by accumulation of hydrophobic clusters....
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PMID: 19995341
PDF is available here.
PDF is available here.
Biochemistry (New York) (75)5 2010
Abstract
We demonstrated that tetrameric BkLAP exists as the major form in solution at protein concentration of 1.5 mg/ml at pH 8.0. The native enzyme started to unfold beyond ~1 M GdnHCl and reached an unfolded intermediate with [GdnHCl](1/2) at 1.8 M. Thermal unfolding of BkLAP was found to be highly irrev...
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PMID: 20632945
PDF is available here.
PDF is available here.
Abstract
This paper is aimed at investigating the conformational change of denatured bovine serum albumin (BSA) in combination with thermodynamic functions and their fractions, adsorption isotherms, Fourier transform infrared (FTIR) spectroscopy, and differential scanning calorimetry (DSC). Microcalorimetric...
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PMID: 20138631
PDF is available here.
PDF is available here.
Abstract
Two different series of oligonucleotide-peptide conjugates have been efficiently synthesized by stepwise solid-phase synthesis. First, oligonucleotides and oligonucleotide phosphorothioates containing polar groups at the 3'-termini, such as amine and guanidinium groups were prepared. ODNs conjugates...
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PMID: 20206515
PDF is available here.
PDF is available here.
Amino Acids (38)4 2010
Abstract
A series of PTH hybrids containing a diamine [NH(2)(CH(2))(n)NH(2); n = 4, 5, 6] in the C-terminal position was synthesized based on the H-Aib-Val-Aib-Glu-Ile-Gln-Leu-Nle-His-Gln-Har-NH(2) (Har = homoarginine) template. The compounds were pharmacologically characterized at PTH1R receptors for agonis...
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PMID: 19690796
PDF is available here.
PDF is available here.
Molecular Vision (16) 2010
Abstract
Our findings highlight the close connections among disulfide bond cleavage and/or formation, intermolecular interactions, and the resultant formation of aggregates of HGDC induced by UV-C irradiation. The results from this research may not only contribute to the understanding of the environmental fa...
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PMID: 21197112
PDF is available here.
PDF is available here.
Journal of Oleo Science (59)2 2010
Abstract
We studied the effects of the surfactants on the solubilisation of liposomes and the release of carboxyfluorescein from liposomes. In addition, the effect of the surfactant molecular structure on the skin irritation was evaluated in connection with the interactions of the surfactants with BSA and li...
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PMID: 20103983
PDF is available here.
PDF is available here.
Arzneimittel-Forschung (60)9 2010
Abstract
Specific serotonin receptor agonists and antagonists are marketed with respect to various diseases, most prominently severe emesis. To identify new chemical classes with affinity for the serotonin 5-HT3 channel, several compounds were synthesized which can be structurally classified as arylalkylamin...
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PMID: 21117497
PDF is available here.
PDF is available here.
PLoS Pathogens (5)11 2009
Abstract
We show that, contrary to expectations, sequential administration of the antiviral inhibitor guanidine (GU) first, followed by ribavirin, is more effective than combination therapy with the two drugs, or than either drug used individually. Coelectroporation experiments suggest that limited inhibitio...
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PMID: 19911056
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)40 2009
Abstract
We are able to gain rough insight into the diverse structural properties of 6 M GdmCl-denatured proteins. It demonstrates that most 6 M GdmCl-denatured proteins are approximately fully denatured, but partially unfolded. Most of them comprise diverse conformational isomers. We review here the cumulat...
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PMID: 19728745
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (113)35 2009
Abstract
We have characterized the collapse of the denatured chain and its relation to structure formation. Two mutants, K28C/H124C and K28C/K97C, were constructed and labeled for monitoring the behaviors of the global molecule and the beta subdomain, respectively. For both the labeled mutants, only native a...
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PMID: 19678648
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)34 2009
Abstract
The quaternary stability of purified, detergent-solubilized, cytochrome c oxidase (CcO) was probed using two chemical denaturants, urea and guanidinium chloride (GdmCl). Each chaotrope induces dissociation of five subunits in a concentration-dependent manner. These five subunits are not scattered ov...
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PMID: 19663452
PDF is available here.
PDF is available here.
Journal of Organic Chemistry (74)15 2009
Abstract
CuI/L5 (N,N'-dimethylethylenediamine) proves to be an efficient catalyst system for the amination of arylhalides with guanidines. The same catalyst system is then successfully applied to the one-step synthesis of 1-H-2-amino-benzimidazoles through tandem aminations of 1,2-dihaloarenes in modest yiel...
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PMID: 19527012
PDF is available here.
PDF is available here.
Abstract
Guanidine compounds have important biochemical properties. Aminoguanidine, as an example, is an anti-oxidant, a nitric oxide synthase inhibitor (NOS) which prevents nitric oxide formation, and an inhibitor of advanced glycosylation end products (AGEs). As an anti-oxidant, aminoguanidine may affect t...
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PMID: 19578741
PDF is available here.
PDF is available here.
Abstract
The overall results further demonstrate the potential utility of these two prosthetic groups for the radiohalogenation of internalizing monoclonal antibodies and their fragments. Specifically, the trastuzumab-derived double mutant fragment in combination with these residualizing agents warrants furt...
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PMID: 19647173
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)30 2009
Abstract
We examined resultant changes in conformation and stability. Human gammaS-crystallin cDNA was cloned into pET-20b(+), and the G18V mutant was generated by site-directed mutagenesis. Recombinant HgammaS-crystallins were expressed in Escherichia coli and purified by ion-exchange and size-exclusion chr...
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PMID: 19558189
PDF is available here.
PDF is available here.
Biophysical Journal (97)1 2009
Abstract
We slow down its folding in a cryogenic water-ethylene-glycol solvent (-18 to -28 degrees C). The refolding kinetics are probed by small-angle x-ray scattering, circular dichroism, and fluorescence to measure the radius of gyration, the average secondary structure content, and the native packing aro...
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PMID: 19580767
PDF is available here.
PDF is available here.
FEBS Letters (583)12 2009
Abstract
Escherichia coli phosphofructokinase-2 (Pfk-2) is a homodimer whose subunits consist of a large domain and an additional beta-sheet that provides the interfacial contacts between the subunits, creating a beta-barrel flattened-like structure with the adjacent subunit's beta-sheet. To...
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PMID: 19465020
PDF is available here.
PDF is available here.
Abstract
We have developed lipid-polycation-DNA (LPD) nanoparticles containing DOTAP and targeted with polyethylene glycol (PEG) tethered with anisamide (AA) to specifically deliver siRNA to H460 human lung carcinoma cells which express the sigma receptor. A novel non-glycerol based cationic lipid which cont...
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PMID: 19267451
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (113)19 2009
Abstract
These results suggest that subdenaturing amounts of GdnHCl restrict the motional freedom of the internal waters, and can have considerable influence on the surface hydration. On increasing the denaturant concentration further, the dispersion amplitude drops sharply, indicating that the chaotropic ac...
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PMID: 19388636
PDF is available here.
PDF is available here.
Abstract
We have monitored the CO vibration following photodissociation with step-scan FT-IR spectroscopy. We have found that like the longer time scale processes, the 50-100 mus time scale process is associated not with protein dynamics but with CO ligand dynamics. The data clearly demonstrate that whatever...
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PMID: 19361220
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (113)10 2009
Abstract
The interactions of ions with a model peptide (a single melittin alpha-helix) in solutions of tetrapropylammonium sulfate or guanidinium chloride were examined by molecular dynamics simulations. The tetrapropylammonium cation shares the geometrical property of essentially flat faces with the previou...
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PMID: 19708168
PDF is available here.
PDF is available here.
Abstract
The influence of N-substituents on the mode of reaction of ortho-quinones generated by oxidation of N-substituted dopamine derivatives has been studied. Ortho-quinones with amide, urea or guanidine side chains are relatively stable, with evidence of rearrangement to para-quinomethanes. The N-methylt...
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PMID: 19225678
PDF is available here.
PDF is available here.
Abstract
A new toxic protein named malanin was isolated from seeds of Malania olei fera by hydrophobic chromatography, whose cytotoxic activities against carcinoma cells were very strong. The conformational changes of malanin at various temperatures, pH, organic solvents, surfactant, denaturant and fluoresce...
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PMID: 19455822
PDF is available here.
PDF is available here.
Abstract
The relatively new technique of vapor pressure osmometry was utilized to determine the preferential interaction of five common solution additives (arginine HCl, guanidine HCl, glycerol, glucose, and urea) using three different model proteins (BSA, lysozyme, and alpha-chymotrypsinogen). Results for g...
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PMID: 19199688
PDF is available here.
PDF is available here.
Abstract
The macrocycle bis-p-xylyl[26]crown-6 (BPX26C6) is capable of complexing guanidinium ions in a [2]pseudorotaxane-like manner in solution. A corresponding molecular switch can be operated through changes in solvent polarity or the addition and removal of halogen and acetate anions....
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PMID: 19105741
PDF is available here.
PDF is available here.
Abstract
A new hybrid permethylated-amine-guanidine ligand based on a 1,3-propanediamine backbone (2L) and its Cu-O2 chemistry is reported. [(2L)CuI(MeCN)]1+ complex readily oxygenates at low temperatures in polar aprotic solvents to form a bis(mu-oxo)dicopper(III) (O) species (2b), similar to the parent bis-...
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PMID: 19119846
PDF is available here.
PDF is available here.
Abstract
Compilation of agmatine structure and imidazoline ring leads to a new family of imidazoline/alpha(2)-adrenoceptor ligands, 4(5)-(2-aminoethyl)imidazoline derivatives. Constraining of the guanidine moiety into heterocyclic ring improved the affinities of the resultant fusion compounds in comparison t...
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PMID: 19101144
PDF is available here.
PDF is available here.
Abstract
Human serum albumin (HSA) participates in heme scavenging, the bound heme turning out to be a reactivity center and a powerful spectroscopic probe. Here, the reversible unfolding of heme-HSA has been investigated by (1)H-NMR relaxometry, circular dichroism, and absorption spectroscopy. In the presen...
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PMID: 18936983
PDF is available here.
PDF is available here.
Abstract
Dodecanoyl amidoalkylguanidine hydrochlorides (C(12)A(m)G, m = 2, 3, 4, 6) are cationic surfactants that have an amidoalkyl group (A(m)) as spacer between the cationic guanidine and hydrophobic groups in the molecule. The effect of the A(m) group on the aggregation properties of the surfactants was...
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PMID: 18990404
PDF is available here.
PDF is available here.
Biophysical Journal (96)3 2009
Abstract
We measured the tensile profile of an alanine-based alpha-helical polypeptide on an atomic-force microscope to investigate the basic mechanics of helix extension with minimal interference from side-chain interactions. The peptide was extended to its maximum contour length with much less force than i...
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PMID: 19186146
PDF is available here.
PDF is available here.
Abstract
We explored the mechanism of high-pressure protein refolding by quantitatively assessing the magnitude of the protein-protein interactions both at atmospheric and elevated pressures for T4 lysozyme, in solutions containing various amounts of guanidinium hydrochloride. At atmospheric pressure, the pr...
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PMID: 18781701
PDF is available here.
PDF is available here.
Abstract
The dimeric form of Torpedo californica acetylcholinesterase provides a valuable experimental system for studying transitions between native, partially unfolded, and unfolded states since long-lived partially unfolded states can be generated by chemical modification of a nonconserved buried cysteine...
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PMID: 19115961
PDF is available here.
PDF is available here.
Journal of Molecular Biology (385)1 2009
Abstract
We report a systematic analyses of unfolding and folding kinetics using single- and double-jump experiments as well as measurements as a function of guanidium chloride, protein, and metal concentration for fully metallated (holo) pseudo wild-type and ALS-associated mutant (E100G, G93R, G93A, and met...
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PMID: 18951903
PDF is available here.
PDF is available here.
Journal of Molecular Biology (385)1 2009
Abstract
The organisation of the structure present in the chemically denatured N-terminal domain of phosphoglycerate kinase (N-PGK) has been determined by paramagnetic relaxation enhancements (PREs) to define the conformational landscape accessible to the domain. Below 2.0 M guanidine hydrochloride (GuHCl),...
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PMID: 18948115
PDF is available here.
PDF is available here.
Abstract
We established a 96-well-plate-based refolding screening system using zeolite. In this system, protein denatured and solubilized with 6 M guanidine hydrochloride is adsorbed onto zeolite placed in a 96-well plate. The refolding conditions can be tested by incubating the samples with refolding buffer...
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PMID: 19462439
PDF is available here.
PDF is available here.
Molecular Vision (15) 2009
Abstract
These results indicate that the interactions offered by E294 in the dimer-dimer interface of delta-crystallin are required to maintain the hydrogen bonding network in the active site for catalysis. Disruption of the interaction had no significant effect on the conformation and quaternary structure o...
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PMID: 19936305
PDF is available here.
PDF is available here.
Abstract
We describe an approach in which two-dimensional electrophoresis (2DE)-separated proteins are guanidinated in-gel prior to enzymatic cleavage. In contrast to previously described techniques, this procedure allows the extracted tryptic peptides to be N-terminally sulfonated without any further sample...
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PMID: 19381605
PDF is available here.
PDF is available here.
Molecular Vision (15) 2009
Abstract
We examined the effects of denaturants and a co-solvent on recombinant TGFBIp, with a focus on protein conformational changes and amyloid fibril formation.
Recombinant TGFBIp was subjected to various spectroscopic studies, such as far-ultraviolet circular dichroism (far-UV CD), intrinsic tryptophan...
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PMID: 20011632
PDF is available here.
PDF is available here.
Abstract
Reaction of the (CH(3))(2)Sn(2+) electrophile toward trilacunary [A-alpha-XW(9)O(34)](n-) Keggin polytungstates (X = P(V), As(V), Si(IV)) with guanidinium as templating-cation resulted in the isostructural compounds Na[C(NH(2))(3)](2)[{(CH(3))(2)Sn(H(2)O)}(3)(A-alpha-PW(9)O(34))] x 9 H(2)O (1), Na[C...
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PMID: 20449121
PDF is available here.
PDF is available here.
Abstract
A new guanidine derivative named plantago-guanidinic acid was isolated from the seeds of Plantago asiatica. The structure was elucidated by two-dimensional (2D) nuclear magnetic resonance (NMR) spectral and other spectral methods.
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PMID: 18665332
PDF is available here.
PDF is available here.
Abstract
The partially unfolded protein is in molten globule-like state, in which approximately 60% and approximately 20% tertiary and secondary structure is disrupted respectively. Despite lacking a fully coordinated tertiary structure for assembling a functional binding cleft, the protein in molten globule...
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PMID: 19463188
PDF is available here.
PDF is available here.
Natural Product Reports (26)2 2009
Abstract
An overview of marine natural products synthesis during 2007 is provided. As with earlier installments in this series, the emphasis is on total syntheses of molecules of contemporary interest, new total syntheses, and syntheses that have resulted in structure confirmation or stereochemical assignmen...
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PMID: 19177223
PDF is available here.
PDF is available here.
Biophysical Chemistry (139)1 2009
Abstract
Our results provide an insight into a stabilization strategy for heme proteins involving variation of the heme electronic state and introduction of appropriate mutations....
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PMID: 18957274
PDF is available here.
PDF is available here.
Journal of Neurochemistry (108)2 2009
Abstract
The ability of G protein-coupled receptors to regulate osmosensitive uptake of the organic osmolyte, taurine, into human SH-SY5Y neuroblastoma cells has been examined. When monitored under isotonic conditions and in the presence of physiologically relevant taurine concentrations (1-100 microM), taur...
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PMID: 19012745
PDF is available here.
PDF is available here.
Abstract
To find out the changes in the internal dynamics and function of proteins as a consequence of their binding interactions with guanidine hydrochloride (GdnHCl), laser flash photolysis and optical absorption methods have been used to study the dynamic events in the horse myoglobin-CO complex (MbCO) in...
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PMID: 18752006
PDF is available here.
PDF is available here.
Abstract
We demonstrate that chiral bicyclic guanidine can catalyze the addition of 1,3-dicarbonyl compounds to 1,4-dicarbonyl but-2-enes [(E)-4-oxo-4-arylbutenamides and (E)-4-oxo-4-arylbutenones] with high regioselectivity and enantioselectivity (ee values up to 97%)....
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PMID: 19301341
PDF is available here.
PDF is available here.