Abstract
A study was conducted to determine the effectiveness of two methods of equipment cleanout, sequencing or flushing, for reducing drug carryover in feedlot mixer trucks. Feed samples were collected from total mixed rations before and after various feed mixer equipment cleanout procedures. Medicated ra...
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PMID: 20051207
PDF is available here.
Abstract
Chlortetracycline, an antibiotic commonly used as a growth promoter in livestock, enters the environment primarily through application of animal waste to open fields. The photochemical loss of chlortetracycline in sunlight-exposed soils is a potentially important process in its environmental fate, e...
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PMID: 19591472
PDF is available here.
BMC Genomics (10 Suppl 2)suppl 2 2009
Abstract
The three antibiotics used in this study, amoxicillin, chlortetracycline, and enrofloxacin, collectively influenced transcription of 25% of the P. multocida Pm70 genome. Some DE genes identified were common to more than one antibiotic. The overall transcription signatures of the three antibiotics di...
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PMID: 19607655
PDF is available here.
Abstract
In the present study, the viability, intracellular pH (pHi), cAMP ([cAMP]i), calcium concentration and protein phosphotyrosine content were evaluated in relation to the acrosomal and capacitation status of freshly ejaculated bull spermatozoa. These parameters were evaluated before and after incubati...
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PMID: 19383258
PDF is available here.
Abstract
These results demonstrate that sperm regulatory mechanisms may be affected by the cryopreservation procedure, but frozen-thawed sperm can still regulate their capacitation and acrosome reaction signalling pathways....
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PMID: 19383259
PDF is available here.
Abstract
We found that while compost windrows did not reach the recommended temperature of 55 degrees C for 15 d, composting reduced high initial levels of total, Amp(r), and Tet(r) E. coli as early as Week 2. A significant antimicrobial effect on total (P = 0.04) and Amp(r) (P = 0.03) E. coli was observed....
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PMID: 19202027
PDF is available here.
Abstract
Feedlots are potential point sources for the flow of antibiotics into the environment due to common use of antibiotics such as sulfamethazine, chlortetracycline and tylosin. Hence soils and manures originating from a grassland control, an experimental and a commercial feedlot were analyzed and mass...
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PMID: 18440678
PDF is available here.
Abstract
The fate of antibiotic residues in the manure of treated animals is of considerable concern because of the potential development of antibiotic-resistant bacteria in the environment. The objective of this study was to determine the fate of chlortetracycline (CTC) during the anaerobic digestion of man...
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PMID: 18353545
PDF is available here.
Abstract
We investigated the change in chlortetracycline resistance in 360 Escherichia coli strains separated from the feces of piglets fed fermented liquid feed (FLF) in comparison with those fed dry feed (control). The total amount of lactic acid bacteria in feces was 8.77 x 10(8) CFU/g DM at weaning, whic...
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PMID: 18708150
PDF is available here.
Abstract
Escherichia coli strains isolated from fecal samples were screened to examine changes in phenotypic and genotypic characteristics including antimicrobial susceptibility, clonal type, and carriage of resistance determinants. The goal of this 197-day study was to investigate the influence of administr...
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PMID: 18723654
PDF is available here.
L J V Piddock,
D Griggs,
M M Johnson,
V Ricci,
N C Elviss,
L K Williams,
F Jørgensen,
S A Chisholm,
A J Lawson,
C Swift,
T J Humphrey and
R J Owen
Abstract
Our data indicate that chlortetracycline treatment does not eradicate tetracycline-resistant Campylobacter spp. from poultry. However, if a low number of resistant isolates are present, then the antibiotic pressure appears insufficient to select such strains as the dominant population....
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PMID: 18467308
PDF is available here.
Abstract
Overall, 56.0% and 31.4% of E coli and Enterococcus isolates, respectively, were resistant to tetracycline. Exposure to chlortetracycline was associated with a significant temporary increase in log(2) MIC for both genera but returned to preexposure values by day 33. Averaged across time, the proport...
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PMID: 18672961
PDF is available here.
Abstract
The pleuromutilin antibiotic tiamulin (TIA) is known to produce a negative interaction in broilers when administered in combination with several ionophore anticoccidials such as salinomycin (SAL). Chlortetracycline (CTC), when administered simultaneously with TIA, has demonstrated a synergistic anti...
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PMID: 18648050
PDF is available here.
Abstract
Eight antibiotics, chlortetracycline, demeclocycline, doxycycline, methacycline, minocycline, oxytetracycline, tetracycline and rolitetracycline, were separated and quantified in Spanish honey extracts of different floral origin using a commercial RP-C18 HPLC column and two different on-line detecto...
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PMID: 18501371
PDF is available here.
Abstract
An automated system using on-line solid-phase extraction (SPE) high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection was developed for the determination of tetracyclines (TCs), such as tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), metacycline (MC), and do...
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PMID: 18585209
PDF is available here.
Abstract
We examined the effects of the protein phosphatase inhibitor calyculin A on the postacrosomal protein serine/threonine phosphorylation state and acrosome morphology in boar spermatozoa incubated with a cAMP analog. Proteins were highly phosphorylated on the serine/threonine residues only in the post...
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PMID: 18305366
PDF is available here.
Abstract
These results suggest that organic matter and cation exchange mainly contribute to the sorption of CTC on manure....
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PMID: 18624208
PDF is available here.
Abstract
A simple reversed flow injection colourimetric procedure for determining iron(III) was proposed. It is based on the reaction between iron(III) with chlortetracycline, resulting in an intense yellow complex with a suitable absorption at 435nm. A 200mul chlortetracycline reagent solution was injected...
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PMID: 18371775
PDF is available here.
Abstract
A rapid and precise determination residues of 4 tetracyclines (TCs) (oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC) and doxycycline (DOXY)) in meat was developed by employing three analyses; a microbiological screening, HPLC and LC/MS/MS. TCs were extracted with pH 4.0 McIlvaine b...
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PMID: 18344657
PDF is available here.
Abstract
We describe the development of a novel screen for PAD4 inhibitors that is based on a PAD4-targeted Activity-Based Protein Profiling reagent, denoted Rhodamine-conjugated F-Amidine (RFA). This screen was validated by screening 10 Disease Modifying Anti-Rheumatic Drugs (DMARDs) and identified streptom...
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PMID: 17964793
PDF is available here.
Abstract
We also detected the transfer of pCAR1 into non-Pseudomonas, Stenotrophomonas-like bacteria....
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PMID: 17851640
PDF is available here.
Abstract
We present a flow cytometric approach for studying Ca2+ alteration in apoptosis at the single cell level. By means of a multiparametric analysis, we could discriminate different sub-populations, i.e., viable and apoptotic cells and cells in secondary necrosis, and separately analyse static as well a...
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PMID: 17899381
PDF is available here.
Abstract
We observed that the coiflets (COIF-CWT) method with dilation parameter, a=400, gives suitable results for this analytical application. For a comparison, the classical derivative spectrophotometry (CDS) approach was also applied to the simultaneous quantitative resolution of the same analytical prob...
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PMID: 17320471
PDF is available here.
Abstract
The objective of this research was to determine the effect of stocking density and inclusion of subtherapeutic chlortetracycline in the diet on Salmonella fecal prevalence and antimicrobial resistance in growing swine. A 2 x 2 factorial design was employed on a privately owned commercial swine farm....
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PMID: 17482387
PDF is available here.
Abstract
Antibiotics and antibiotic metabolites have been found in the environment, but the biological activities of these compounds are uncertain, especially given the low levels that are typically detected in the environment. The objective of this study was to estimate the selection potential of chlortetra...
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PMID: 17616621
PDF is available here.
Abstract
In an effort to identify a more suitable antibiotic for utilization in mycological media, 12 food borne fungal species from various genera including Alternaria alternata, Aspergillus flavus, A. niger, Eurotium chevalieri, Fusarium moniliforme, Penicillium sp., Rhizopus stolonifer, Saccharomyces cere...
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PMID: 17574697
PDF is available here.
Abstract
We have tested how capacitation of frozen-thawed bull spermatozoa is effected by exposure to different GAGs detectable or possibly present in oviductal fluid; i.e. heparin, hyaluronan, heparan sulphate, dermatan sulphate and chondroitin sulphate. Following exposure of different duration, the spermat...
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PMID: 17637103
PDF is available here.
Abstract
The possible role of the primary Buchnera symbionts in wing polyphenism is examined in three aphid species. Presumptive winged aphids were fed on antibiotic-treated beans to destroy these symbionts. As previously reported, this leads to inhibited growth and low/zero fecundity. When such treatment is...
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PMID: 17628287
PDF is available here.
Abstract
Antibiotics have traditionally been used for growth promotion in the pork industry; however, their use in animal feed has recently been limited because of human health concerns. The intestinal microbiota plays an important role in mediating many physiological functions such as digestion and animal g...
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PMID: 17400973
PDF is available here.
Abstract
Glutathione S-transferases (GSTs) isolated from chlortetracycline (CTC)-treated maize catalyzed the conjugation of glutathione (GSH) with CTC, producing stable conjugates that were structurally characterized using liquid chromatography-ion-trap mass spectrometry (LC-IT-MS). Enzyme-mediated dechlorin...
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PMID: 17592585
PDF is available here.
Abstract
We obtained multiple samples of ejaculated spermatozoa from normal fertile C57BL/6 and infertile Hook1/Hook1 (formerly known as azh/azh) mutant males from uteri after mating, thus avoiding termination of the males. Ejaculated sperm were preserved by conventional cryopreservation or by rapid freezing...
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PMID: 17314312
PDF is available here.
Abstract
Tetracycline (TC) and chlortetracycline (CTC) are used extensively for growth promotion and therapeutic purposes in livestock production. The sorption of TC and CTC on clays, humic substances (HS), and clay-humic complexes (clay-HC) derived from two agricultural soils was quantified using dilute CaC...
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PMID: 17410786
PDF is available here.
Abstract
Efficient methods are needed for analysis of veterinary drug residues in food. A number of methods are available for single analytes. Multiresidue methods are now increasingly available. It is still rare, however, to find methods not involving mass spectrometry which allow for analysis of more than...
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PMID: 17386722
PDF is available here.
Abstract
We performed an image analysis of cells loaded with the fluorescent dye chlortetracycline (CTC) to in situ analyze Ca2+ changes within the ER in apoptosing promonocytic U937 cells. The results, validated through the use of thapsigargin (THG) as ER Ca2+ depletor, confirm the findings that apoptotic c...
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PMID: 17446492
PDF is available here.
Abstract
We examined several of the predictions of the amplification-dependent mutation model and found that they are not fulfilled. First, inhibition of adaptive mutation by a gene that is toxic when overexpressed does not depend on the proximity of the gene to lac. Second, mutation at a second locus during...
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PMID: 17209030
PDF is available here.
Abstract
These results suggest that maize is able to detoxify chlortetracycline via the glutathione pathway, whereas pinto beans cannot. This may explain the observed stunted growth of pinto beans after antibiotic treatment. This study demonstrates the importance of plant uptake in determining the fate of an...
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PMID: 17593756
PDF is available here.
Abstract
A rapid, specific, and sensitive procedure for determining residues of 4 widely used tetracycline antibiotics and 3 of their 4-epimers in cheese is presented. The method is based on the matrix solid-phase dispersion (MSPD) technique followed by liquid chromatography/tandem mass spectrometry (LC/MS/M...
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PMID: 17580641
PDF is available here.
Abstract
Retained fetal membranes (RFM) and clinical metritis (CM) are frequently diagnosed disease conditions in dairy cows and considered of major economic impact due to negative effect on reproduction and milk production. The objective of this study was to evaluate the efficacy of i.u. tetracycline for th...
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PMID: 16962164
PDF is available here.
Abstract
Chlortetracycline and the macrolide tylosin were identified as commonly used antimicrobials for growth promotion and prophylaxis in swine production. Resistance to these antimicrobials was measured throughout the waste treatment processes at five swine farms by culture-based and molecular methods. C...
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PMID: 17041160
PDF is available here.
Abstract
We present an LC-electrospray ionization (ESI)-MS/MS method to analyze the CTC content in pig tissues, with the originality that it apports a solution to overcome the possible interference on the measurement of the keto-enol tautomerism equilibrium, which is influenced by several factors. Therefore,...
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PMID: 16938306
PDF is available here.
Abstract
We validate the chlortetracycline (CTC) technique for the evaluation of capacitation and acrosome reaction-like changes in ram sperm, carrying out a double estimation of the acrosome status after treatment with lysophosphatidylcholine, using fluorescein isocyanate (FITC)-RCA/ethidium homodimer 1 (Et...
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PMID: 17071773
PDF is available here.
Abstract
A total of 18 chlamydial isolates from various psittacine birds, one isolate from a domestic pigeon and one isolate from a Pekin duck were isolated in continuous Buffalo Green Monkey (BGM) kidney cell cultures. All 20 isolates were identified by nested multiplex polymerase chain reaction as Chlamydo...
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PMID: 17147151
PDF is available here.
Abstract
A total of 90 weaned female pigs (Duroc x Landrace x Yorkshire) were used in a 15-d growth experiment to investigate the effect of lactoferrin on growth performance, intestinal morphology, and expression of PR-39 and protegrin-1 genes. The pigs were allocated on the basis of BW and litter to 3 dieta...
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PMID: 16971564
PDF is available here.
Abstract
Effects of tetracycline residues from pig manure slurry on the prevalence of tetracycline-resistant bacteria and the tetracycline resistance gene, tet(M), were studied in soil microcosms. Four types of soil microcosms were established for a period of 152 days, supplemented with combinations of pig m...
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PMID: 16808974
PDF is available here.
Abstract
Chlortetracycline is an antibiotic that is used to increase weight gain, efficiency of gain, carcass grade, and conception rates. The objective of this experiment was to evaluate the effects of supplementation of 350 mg/d of chlortetracycline on ADG, G:F, BCS, thyroxine, and systemic glucose concent...
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PMID: 16908644
PDF is available here.
Abstract
In this study, the effects of two different calcium channel blockers, diltiazem and verapamil on calcium uptake and release from the membrane of heavy sarcoplasmic reticulum (SR) of chicken skeletal muscle were investigated. A fluorescent chelate probe technique was employed to determine calcium mov...
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PMID: 16889071
PDF is available here.
Abstract
A disease challenge experiment was conducted to determine if including 10% dried distillers grains with solubles (DDGS) in the diet, with or without antimicrobial supplementation, reduces the incidence or severity, or both, of intestinal lesions in growing pigs after an Lawsonia intracellularis chal...
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PMID: 16775071
PDF is available here.
Abstract
Western blotting revealed proteins of the correct size for all these receptors; immunolocalization indicated their presence on the head, especially acrosomal and neck regions, and flagellum of both mouse and human spermatozoa. CTC results indicated significant responses to agonists for all of the be...
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PMID: 16488904
PDF is available here.
Abstract
We analyzed the Pasteurella multocida proteome response to sub-MICs of amoxicillin, chlortetracycline, and enrofloxacin using isotope-coded affinity tags (ICAT). There were parallel effects on inhibition of growth kinetics and suppression of protein expression by clusters of orthologous groups (COG)...
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PMID: 16512672
PDF is available here.
Abstract
We demonstrate here that mechanical shaking and caffeine, the ryanodine-receptor agonist, induced an elevation of cytosolic calcium in the dinoflagellate Crypthecodinium cohnii. Dantrolene, a ryanodine-receptor antagonist, dose-dependently inhibited both shaking-induced and caffeine-induced calcium...
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PMID: 16364435
PDF is available here.
Abstract
Five patients in the treatment group (45%) achieved an ACR20 response at 1 year compared to none in the control group (p = 0.04). Eight patients in the treatment group and 1 in the control group had a greater than 20% improvement in tender joint count (p = 0.008). There were also significant differe...
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PMID: 16465651
PDF is available here.
Abstract
We present an LC-ESI-MS/MS method to analyze the CTC content in animal plasma, with the originality that it apports a solution to overcome the possible interference of the keto-enol tautomerism equilibrium, which is influenced by several factors. Therefore, the plasma samples were incubated at 56 de...
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PMID: 16246348
PDF is available here.
Abstract
This paper reports an indirect flow-injection (FI) method for the determination of the tetracycline drugs (TCs), tetracycline (TC), chlortetracycline (CTC) and oxytetracycline (OTC), using copper(II) as a probe ion. The method was based on the inhibition caused by these TCs to the copper(II)-catalys...
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PMID: 16416504
PDF is available here.
Abstract
The aims of this research were to determine the association between inclusion of subtherapeutic chlortetracycline in the diets of swine and the prevalence and antimicrobial resistance of Salmonella; define the association between inclusion of subtherapeutic chlortetracycline and antimicrobial resist...
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PMID: 17002549
PDF is available here.
Abstract
We observed that heparin binding sites were located mostly on the acrosomal region of boar sperm in an specific and saturable manner. The in vitro effect of heparin described in this work indicates that sulfated glycosaminoglycans, which are normally present in the female reproductive tract, might p...
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PMID: 17657344
PDF is available here.
Abstract
A robust high-throughput method was refined to extract three growth-promoting antibiotics, tylosin (TYL), chlortetracycline (CTC), and monensin (MON), from soil. Analysis was performed by electrospray liquid chromatography tandem mass spectrometry. Soil dissipation rate studies were performed in a f...
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PMID: 16494218
PDF is available here.
Abstract
These results suggest that 24-h soil and antibiotic solution mixtures is enough for sorption studies. Also, there is less likelihood that these antibiotics will leach through soil and appear in the ground water since their sorption on soils is very high unless they are carried by soil particles thro...
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PMID: 16738380
PDF is available here.