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Amido Black (0):
Recent article
Amido Black
Abstract
We have found that at the protein concentrations normally used to quantify most low-abundance proteins of interest, frequently used single-protein loading controls, such as glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and beta-actin, do not accurately reflect differences in protein concentration...
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PMID: 18571732
PDF is available here.
PDF is available here.
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Abstract
Research into fingermark enhancement techniques has traditionally used visual comparisons and qualitative methods to assess their effectiveness based on the quality of the developed fingermark. However, with increasing research into the optimisation of these techniques the need for a quantitative ev...
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PMID: 18359590
PDF is available here.
PDF is available here.
Journal of Physical Chemistry B (110)41 2006
Abstract
We describe the use of Nano Orange, a fluorometric assay, to quantitatively assess the adsorption of bovine fibrinogen and albumin onto model hydrophilic (OH terminated) and hydrophobic (CH3 terminated) surfaces. Results obtained using this method allowed the calibration of previously unquantifiable...
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PMID: 17034246
PDF is available here.
PDF is available here.
FEMS Microbiology Letters (99)2-3 2006
Abstract
Azo dye are recalcitrant pollutants. Two sulfonated azo dyes, Orange II and Amino black, are effectively decolorized by certain noncardioform strains of the genus Rodococcus. A mutant of one of these strains was isolated which had lost azo-dye decolorizing ability and the strain was used to clone DNA...
PDF is available here.
PDF is available here.
Chemosphere (59)6 2005
Abstract
Brewer's yeast (bottom yeast, Saccharomyces cerevisiae subsp. uvarum) cells were magnetically modified using water based magnetic fluid stabilized with perchloric acid. Magnetically modified yeast cells efficiently adsorbed various water soluble dyes. The dyes adsorption can be described by the Lang...
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PMID: 15811411
PDF is available here.
PDF is available here.
Biotechnology Letters (27)5 2005
Abstract
Cyanobacterial cultures isolated from sites polluted by industrial textile effluents were screened for their ability to decolorize cyclic azo dyes. Gloeocapsa pleurocapsoides and Phormidium ceylanicum decolorized Acid Red 97 and FF Sky Blue dyes by more than 80% after 26 days. Chroococcus minutus wa...
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PMID: 15834793
PDF is available here.
PDF is available here.
Analytical Biochemistry (338)1 2005
Abstract
Water Research (39)1 2005
Abstract
A municipal wastewater treatment plant (WWTP) receiving industrial dyeing discharge containing acid black 1 (AB1) failed to meet NH(3) and BOD(5) discharge limits, especially for NH(3) during the winter. Dyeing discharge was combined with domestic sewage in volumetric ratios reflecting the range rec...
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PMID: 15607160
PDF is available here.
PDF is available here.
Electrophoresis (25)15 2004
Abstract
A new formulation of the small-molecule organic fluorophore, Pro-Q Diamond dye, has been developed that permits rapid and simple detection of phosphoproteins directly on polyvinylidene difluoride (PVDF) or nitrocellulose membranes (electroblots). Protein samples are first separated b...
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PMID: 15300773
PDF is available here.
PDF is available here.
Abstract
A combined surface sampling probe/electrospray emitter coupled with an ion trap mass spectrometer was used for the direct read out of unmodified reversed-phase C18 thin-layer chromatography (TLC) plates. The operation of the surface sampling electrospray ionization interface in positive and negative...
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PMID: 15174184
PDF is available here.
PDF is available here.
Abstract
A method to detect and enumerate bacterial colonies grown on membrane filters (MF) was described. The colonies were stained with an ethanolic solution of 0.1% Amido black 10B. The procedure yielded the rapid detection of colonies as compared to a conventional plate counting method.
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PMID: 14529960
PDF is available here.
PDF is available here.
Abstract
We have developed a panel of analytical techniques to characterize these lipopolyplexes. Complexes were measured for size by dynamic light scattering and surface-charge characteristics by zeta potential. Interaction between DNA and the polycation, protamine sulphate, was determined using a PicoGreen...
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PMID: 12149118
PDF is available here.
PDF is available here.
BioTechniques (31)3 2001
Abstract
Audio, Transactions of the IRE Professional Group on (Chapter 10) 2001
Abstract
Staining of blot transfer membranes permits visualization of proteins and allows the extent of transfer to be monitored. In the protocols described in this unit, proteins are stained after electroblotting from one-dimensional or two-dimensional polyacrylamide gels to blot membranes s...
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PMID: 18429099
PDF is available here.
PDF is available here.
Abstract
A sequential anaerobic aerobic treatment process based on mixed culture of bacteria isolated from textile dye effluent-contaminated soil was used to degrade sulfonated azo dyes Orange G (OG), Amido black 10B (AB), Direct red 4BS (DR) and Congo red (CR). Under anaerobic conditions in a fixed-bed colu...
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PMID: 10968644
PDF is available here.
PDF is available here.
Analytical Biochemistry (271)2 1999
Abstract
Abstract
Cytochemical method for selective detection of the leukocyte lysosomal and cationic proteins by Amido Black 10B at pH 8.1-8.2 is described. A much better color contrast of the cytological picture in comparison with Stable Green FCF staining facilitates visual assessment of the results and creation o...
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PMID: 10876686
PDF is available here.
PDF is available here.
Abstract
We suggest that the experimental conditions of cytotoxicity testing have to be relevant to the in vivo situation; accordingly, the time of exposure should be designed carefully....
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PMID: 9827670
PDF is available here.
PDF is available here.
Abstract
Annals of Clinical Biochemistry (34 ( Pt 4)) 1997
Abstract
Our data suggest that the procedure employing modified Coomassie brilliant blue may be a suitable alternative to the traditional procedure commonly used in many clinical laboratories....
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PMID: 9247668
PDF is available here.
PDF is available here.
Analytical Biochemistry (248)1 1997
Abstract
Abstract
Analytical Biochemistry (247)2 1997
Abstract
We present a technique for the sensitive staining of proteins electroblotted onto nitrocellulose or polyvinylidene difluoride membranes and enzymatic cleavage conditions for such proteins to achieve optimal recovery of peptides. The technique is based on the deposition of colloidal silver on the mem...
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PMID: 9177693
PDF is available here.
PDF is available here.
Cell and Tissue Research (288)1 1997
Abstract
We describe a simple sensitive method for protein quantification circumventing all these restrictions. Proteins in solution or suspension in any buffer are spotted onto cellulose acetate, dried, and stained with Amido Black. After washing off the excess dye, bound Amido Black is solubilized in an ac...
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PMID: 9042779
PDF is available here.
PDF is available here.
Analytical Biochemistry (245)2 1997
Abstract
Biomaterials (17)13 1996
Abstract
Cell viability and growth for cytotoxicity evaluation of materials for prosthetic devices has been tested using various methods. The aim of this study was to extend the choice of reliable methods to quantify cytotoxicity of materials in vitro. By measuring both viability and growth of cells exposed...
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PMID: 8805972
PDF is available here.
PDF is available here.
Abstract
The present study provides an alternative method for protein-gel drying. Rat brain protein extracts were separated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by staining with coomassie brilliant blue (R-250). The stained gel was then subjected to electroblott...
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PMID: 8607869
PDF is available here.
PDF is available here.
Abstract
A missing person report was filed with the local Police Department. In the ensuing days it became apparent this was not just a missing person case when a bloody pillow and pillowcase were recovered from a wooded area not far from the missing woman's home. Careful examination of the pillowcase reveal...
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PMID: 7595308
PDF is available here.
PDF is available here.
Abstract
Histochemical staining of the granules of eosinophilic granulocytes and subsequent blockade of the reaction by alkaline benzyl was strongly suggestive that in its purified form, the diazo dye naphthalene black reacts with tissue sites containing high concentrations of arginine residues. Computer gra...
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PMID: 7520759
PDF is available here.
PDF is available here.
Abstract
Abstract
Modern biological experiments, conducted on mini- and micro-scales require methods for protein detection in small samples. The method proposed here is based on visual inspection of protein dots immobilized on nitrocellulose filters and stained with the common protein dye amido black. It allows detec...
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PMID: 7525159
PDF is available here.
PDF is available here.
Journal of Immunological Methods (167)1-2 1994
Abstract
A new multipurpose cell micro-assay has been developed, using the protein dye amido black 10B as an indicator of cell numbers in 96-well plates. The assay is reliable, rapidly performed and can be combined with morphological evaluation and photography of stained cells. It permits investigations of v...
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PMID: 7508474
PDF is available here.
PDF is available here.
Abstract
Three stains, Hansel's stain, alkaline erythrocin B (AEB) and naphthalene black (NB), were used to demonstrate eosinophils in the urine of patients infected with Schistosoma haematobium. Hansel's stain was superior to the other two stains; it stained eosinophils bright red and their nuclei faint blu...
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PMID: 7687881
PDF is available here.
PDF is available here.
Abstract
FEMS Microbiology Letters (78)2-3 1992
Abstract
Azo dyes are recalcitrant pollutants. Two sulfonated azo dyes, Orange II and Amido black, are effectively decolorized by certain nocardioform strains of the genus Rhodococcus. A mutant of one of these strains was isolated which had lost azo-dye decolorizing ability and the strain was used to clone D...
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PMID: 1490602
PDF is available here.
PDF is available here.
Abstract
Samples of amido black, Coomassie blue G, and Coomassie blue R obtained over a number of years were tested for dye content, impurities, and effectiveness for staining proteins after polyacrylamide gel electrophoresis and for protein dye-binding assays. Some impurities produced reactions resembling m...
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PMID: 1380317
PDF is available here.
PDF is available here.
Analytical Biochemistry (186)1 1990
Abstract
Copper iodide staining and determination of proteins adsorbed to polystyrene microtiter plates are described. The minimum amount of copper iodide-stained protein detected in densitometric measurements is approximately 20 pg/mm2. Enzyme immunoassay readers may also be used for the determination of co...
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PMID: 1694063
PDF is available here.
PDF is available here.
Abstract
A rapid and sensitive method for protein determination (0.5-16 micrograms) in samples of any volume containing various surfactants in concentration up to 1% is suggested. The method includes the protein acid denaturation, the solution of acid insoluble precipitate of detergent in ethanol (25-30%), t...
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PMID: 2393681
PDF is available here.
PDF is available here.
Abstract
We hereby describe electrophoretic analysis of normal human serum pre-stained with Coomassie Brilliant Blue R-250 (CBB). The proposed method was optimized by studying as many as 450 disc electrophoretic separations and 30 variables. The method when compared with the post-electrophoretic staining by...
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PMID: 2483718
PDF is available here.
PDF is available here.
Journal of Cell Science (93 ( Pt 1)) 1989
Abstract
This paper describes two applications of the tandem scanning reflected-light microscope (TSM) for the observation of the structure of individual cells growing in tissue culture. First, the TSM is used as an alternative to interference reflection microscopy (IRM) or total internal reflection aqueous...
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PMID: 2482294
PDF is available here.
PDF is available here.
Archives of Oral Biology (34)6 1989
Abstract
Experimental fluorosis was induced in order to get information on enamel protein glycosylation, using Western-blotting methodology with peroxidase-labelled concanavalin A. Fluoride inhibited amelogenin degradation, especially the production of intermediate forms. Within the non-amelogenin family of...
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PMID: 2480772
PDF is available here.
PDF is available here.
Annals of dentistry (48)1 1989
Abstract
A tubular adenoid cystic carcinoma of the soft palate was studied histochemically using tannic acid-phosphomolybdic acid-Azophloxine (TPAzf), tannic acid-phosphomolybdic acid-Amidoblack (TPA) and tannic acid-phosphomolybdic acid-Acid Blue techniques. At the periphery of some tumor nests a few spindl...
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PMID: 2551212
PDF is available here.
PDF is available here.
Abstract
Abstract
Abstract
Abstract
We report a method for the staining of proteins on nitrocellulose membranes immediately after electroblotting with Amido Black 10B under mild conditions which do not result in loss of antigenic activity. These membranes can then be probed with immunological detection reagents and the detected antige...
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PMID: 2444652
PDF is available here.
PDF is available here.
Analytical Biochemistry (162)1 1987
Abstract
A convenient method is described by which the actual or relative number of cells in anchorage culture is determined. After removal of the growth medium, cells are subjected to a double-fixation procedure. The cellular protein content is subsequently quantitatively stained with naphthol blue-black. A...
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PMID: 3605582
PDF is available here.
PDF is available here.
Stain technology (61)6 1986
Abstract
The tannic acid-phosphomolybdic acid-amido black (TPA) stain has been used primarily for staining hemoglobin. That different dye lots of amido black cause variable staining is documented in the literature. Nine commercial samples of amido black were investigated using thin layer chromatography; all...
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PMID: 2432696
PDF is available here.
PDF is available here.
Histochemistry (85)5 1986
Abstract
These results are in accordance with those previously obtained in basal-cell epitheliomas....
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PMID: 2430917
PDF is available here.
PDF is available here.
Abstract
Journal of oral medicine (41)3 1986
Abstract
Analytical Biochemistry (150)1 1985
Abstract
We show that in the presence of protein (20 micrograms) and lipid (20 mg), high concentrations of various buffers, salts, and nonionic detergents do not interfere with the assay. Finally, the enhanced ability of this new method to tolerate high lipid levels without interference relative to several e...
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PMID: 4083487
PDF is available here.
PDF is available here.
Clinica Chimica Acta (147)2 1985
Abstract
Analytical Biochemistry (141)1 1984
Abstract
A sodium dodecyl sulfate-polyacrylamide slab-gel system was used to study the alkaline extraction of Naphthol Blue Black B-stained polypeptides. A simple, rapid, and reproducible method for quantitative assessment of 1- to 5-micrograms quantities of individual polypeptides in the molecular weight ra...
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PMID: 6208809
PDF is available here.
PDF is available here.
Analytical Biochemistry (139)2 1984
Abstract
Protein content of a wide variety of sample types may be determined by adsorption of microliter samples onto nitrocellulose, followed by amido black staining and laser densitometry. The method is quite rapid, sensitive, and selective, and dispenses with precipitation and transfer steps.
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PMID: 6206744
PDF is available here.
PDF is available here.
Histochemistry (80)4 1984
Abstract
Conditions are described by which cells and fresh frozen tissues, following fixation with ethanol-ether, and after staining with the amidoblack (AB)-TCA-staining method, show a modified dye/protein ratio of 0.9 moles AB/10(5) g protein compared to 8.5 moles AB/10(5) g protein ( Schauenstein et al. 1...
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PMID: 6203879
PDF is available here.
PDF is available here.
Arkhiv Patologii (46)8 1984
Abstract
Three methods for the detection of generalized intravascular coagulation are proposed. The dyes produced in the USSR are used in these methods: acid red 2C--orange-red-blue method; acid yellow light-proof--picric red-blue method; acid blue-black in the method called red-blue-black. The use of these...
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PMID: 6497702
PDF is available here.
PDF is available here.
Clinical Chemistry (29)1 1983
Abstract
We describe the pre-staining of proteins in normal human serum with Remazol Brilliant Blue before separation by disc electrophoresis. Critical to reproducibility are: dye concentration of 0.16 mol/L in a Tris-glycine buffer (pH 8.3), use of equal volumes of serum and dye solution, a tagging period o...
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PMID: 6848279
PDF is available here.
PDF is available here.
Abstract