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Congo Red (0):
Recent article
Congo Red
Abstract
The bleaching of Congo red was studied in the presence of zinc sulphide nanoparticles doped with Co2+ ion. The nanoparticles of zinc sulphide, doped with cobalt, were synthesized by controlled coprecipitation in the presence of mercaptoethanol as a capping agent. The characterization of nanoparticle...
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PMID: 21046948
PDF is available here.
PDF is available here.
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Abstract
We report an unusual case of a 76-year-old woman with primary cutaneous amyloidosis who initially presented with features of asteatotic eczema that was unresponsive to topical corticosteroid treatment. Histological examination revealed amyloid deposits involving the superficial and deep dermis. Thes...
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PMID: 20938601
PDF is available here.
PDF is available here.
Biophysical Journal (99)4 2010
Abstract
We previously generated a toxic mutant of the nontoxic HET-s((218-289)) amyloid in yeast. Here we report that toxic and nontoxic amyloids differ not only in their structures but also in their assembling process. We used multiple and complementary methods to investigate the intermediates formed by th...
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PMID: 20713008
PDF is available here.
PDF is available here.
Journal of Hazardous Materials (178)1-3 2010
Abstract
In this work, the extraction of Congo red (CR), an anionic disazo direct dye, from aqueous solutions by emulsion liquid membrane (ELM) was investigated. The important operational parameters governing emulsion stability and extraction behavior of dye were studied. The extraction of CR was influenced...
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PMID: 20211520
PDF is available here.
PDF is available here.
Neurobiology of Aging (31)6 2010
Abstract
We demonstrate that soluble, intermediate size oligomers of alpha-synuclein co-elute with the 26S proteasome. These soluble oligomers associate with the 26S proteasome and are significantly increased following treatment with proteasomal, but not lysosomal, inhibitors, indicating specific degradation...
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PMID: 18715677
PDF is available here.
PDF is available here.
Microbiology (156)Pt 5 2010
Abstract
We show that the deletion of sdiA from EHEC O157 : H7 strain 86-24, and from a hha (a negative regulator of ler) mutant of this strain, enhanced bacterial adherence to HEp-2 epithelial cells of the sdiA mutant strains relative to the strains containing a wild-type copy of sdiA. Quantitative reverse...
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PMID: 20110300
PDF is available here.
PDF is available here.
Kidney International (77)9 2010
Abstract
We received 285 renal amyloid samples, of which 31 remained unclassified. In an effort to determine whether any of the latter samples were LECT2 related, tandem mass spectrometry was performed. In all, 7 of the 31 cases were identified as an amyloid LECT2 (ALECT2), a finding confirmed immunohistoche...
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PMID: 20182418
PDF is available here.
PDF is available here.
Abstract
Congo red stain is currently the gold standard for amyloid detection and the goal is to detect amyloid early. Diagnosis of the amyloid type must be based on the identification of amyloid protein within the deposits and not solely by reliance on clinical or DNA studies. However, the latter are recomm...
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PMID: 20367306
PDF is available here.
PDF is available here.
British Journal of Nutrition (103)3 2010
Abstract
The present study aimed to investigate the role of Zn alone and in the presence of ascorbic acid (AA) and folic acid (FA) in albumin glycation. Glycation was performed by incubations of bovine serum albumin with glucose at 37 degrees C along with Zn, AA or FA separately and Zn + AA or Zn + FA for 15...
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PMID: 19849871
PDF is available here.
PDF is available here.
Abstract
We characterized the physicochemical properties of this protein. We report that purified TasA forms fibers of variable length and 10-15 nm in width. Biochemical analyses, in combination with the use of specific dyes and microscopic analyses, indicate that TasA forms amyloid fibers. Consistent with t...
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PMID: 20080671
PDF is available here.
PDF is available here.
Abstract
Historically, heart, liver, and kidney biopsies were performed to demonstrate amyloid deposits in amyloidosis. Since the clinical presentation of this disease is so variable and non-specific, the associated risks of these biopsies are too great for the diagnostic yield. Other sites that have a lower...
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PMID: 21085098
PDF is available here.
PDF is available here.
Abstract
This study examined the removal of acidic indigo carmine dyes from aqueous solutions using cationic hydrogels. Irradiated hydrogels were investigated as a new sorbent for dye removal from aqueous solution. Poly(N,N-Diethylamino ethyl methacrylate) [poly(DEAEMA)] hydrogels were prepared by radiation...
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PMID: 20389008
PDF is available here.
PDF is available here.
Acta Cardiologica (64)6 2009
Abstract
We report an unusual case of a 47-year-old Caucasian woman who presented with severe dyspnoea as a manifestation of restrictive cardiomyopathy, found to be due to myocardial deposition of kappa light chains. Non-routine specific immunofluorescence stainings of endomyocardial biopsy specimens were ke...
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PMID: 20128164
PDF is available here.
PDF is available here.
Der Pathologe (30 Suppl 2)s2 2009
Abstract
The systemic amyloidoses comprise a large group of serious protein deposition diseases. Although rather rare, the disorders are spread all over the world. Increasing understanding of pathogenic mechanisms and recent hope for treatment options demand further research and development of diagnostic pro...
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PMID: 19756622
PDF is available here.
PDF is available here.
Abstract
We tested the influence of trehalose, sucrose and trimethylamine-N-oxide (TMAO) on Abeta aggregation and fluorescent dye leakage induced by Abeta aggregates from liposomes. In the absence of lipid vesicles, trehalose and sucrose, but not TMAO, were found to delay Abeta aggregation. In contrast, all...
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PMID: 19637920
PDF is available here.
PDF is available here.
Biochemistry (Washington) (48)35 2009
Abstract
We present here a broad biophysical approach that enables us to characterize the mechanisms of interaction between alpha-synuclein, a protein whose aggregation is closely connected with Parkinson's disease, and two small molecules, Congo red and Lacmoid, which inhibit its fibrillization. Both compou...
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PMID: 19645507
PDF is available here.
PDF is available here.
Biophysical Journal (97)1 2009
Abstract
We focused our study on the Glu22 residue, comparing the effects of freshly prepared samples and samples aged for at least 20 days. In the aged samples, a high propensity for aggregation and beta-sheet secondary structure appears when residue 22 is capable of establishing polar (Glu22 in wild-type)...
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PMID: 19580765
PDF is available here.
PDF is available here.
Anticancer Research (29)7 2009
Abstract
The effect of doxorubicin and Congo Red on prion protein (PrP) infectivity in experimental scrapie was studied to better understand the effect of these compounds in prion diseases and to establish whether a dose-response correlation exists for Congo Red. This was performed in order to test the effec...
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PMID: 19596920
PDF is available here.
PDF is available here.
Abstract
We investigate the electrohydrodynamic and nanomechanical characteristics of two Saccharomyces cerevisiae yeast strains, a wild-type (WT) strain and a strain overexpressing (OE) Hsp12p, in the presence and absence of hydrophobic Congo red compound. By combining these two advanced biophysical methods...
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PMID: 19416105
PDF is available here.
PDF is available here.
Der Pathologe (30)3 2009
Abstract
Amyloid is a pathologic fibrillar aggregation of polypeptides in a cross-beta-sheet conformation. Amyloidoses are caused by the deposition of amyloid and may occur as cerebral and extracerebral disease. More than 29 different amyloid proteins have been identified. Analysis of a Congo red-stained tis...
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PMID: 19319537
PDF is available here.
PDF is available here.
Der Pathologe (30)3 2009
Abstract
We were able to examine eight patients suspected of LGMD2B clinically, histochemically. The genotype was determined in every case. We found sarcolemmal and interstitial amyloid deposits in four muscle sections. All of the mutations associated with amyloid were located in the N-terminal region of dys...
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PMID: 19326120
PDF is available here.
PDF is available here.
Biophysical Journal (96)6 2009
Abstract
We suggest that fibril formation may take place through the role of association-competent oligomeric intermediates, resulting in a kinetic pathway via clustering of these oligomeric species to yield protofibrils and then fibrils. The resultant fibrils are elongated but curly, and differ in length de...
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PMID: 19289061
PDF is available here.
PDF is available here.
Neuropathology (29)1 2009
Abstract
We also concluded that foci with no intravenous contrast enhancement represent the amyloid deposits. The patient underwent trans-sphenoidal resection of the pituitary adenoma. Histological examination revealed an adenoma with spheroid amyloid deposits adjacent to prolactin-staining adenoma cells. Th...
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PMID: 18498287
PDF is available here.
PDF is available here.
BMC Microbiology (9)1 2009
Abstract
Surface motility was observed on minimal defined agar plates with 0.5% agarose, similar in nature to swarming motility identified in Pseudomonas aeruginosa PAO1. We examined this motility under several culture conditions, including inhibition of flagellar motility using Congo Red. We demonstrated th...
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PMID: 19523213
PDF is available here.
PDF is available here.
Abstract
The fungi displayed significant variation in their resistance to osmotic (NaCl and sorbitol), oxidative (H2O2 and menadione) and cell wall stresses (Calcofluor White and Congo Red). There was no strict correlation between fungal phylogeny and stress resistance. Rather, the human pathogens tended to...
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PMID: 19232129
PDF is available here.
PDF is available here.
Abstract
A series of surfactant-modified montmorillonites (MMT) were prepared using octyltrimethylammonium bromide (OTAB), dodecyltrimethylammonium bromide (DTAB), cetyltrimethylammonium bromide (CTAB) and stearyltrimethylammonium bromide (STAB), and the organification of MMT was proved by Fourier transform...
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PMID: 18400385
PDF is available here.
PDF is available here.
Environmental Microbiology (10)10 2008
Abstract
We report that Pseudomonas putida deficient in ColR experiences serious carbon source-specific stress that leads to the lysis of a subpopulation of bacteria growing on solid glucose medium. We observed that on glucose medium colR-deficient bacteria aggregated, produced a Congo Red-binding substance...
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PMID: 18657172
PDF is available here.
PDF is available here.
Bioresource Technology (99)15 2008
Abstract
Synthetic dyes are important chemical pollutants from various industries. This work developed an efficient and relatively simple continuous decolorization system rice hull-Schizophyllum sp. F17 under solid-state condition in a packed-bed bioreactor, for decolorizing Congo red. In the decolorization...
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PMID: 18328697
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1778)10 2008
Abstract
We further test whether FP may exhibit 'amyloid-like' characteristics, by contrasting its structural and functional properties with those of Abeta(26-42), a 17-residue peptide from the C-terminus of the amyloid-beta protein responsible for Alzheimer's. FTIR spectroscopy, electron microscopy, light s...
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PMID: 18515070
PDF is available here.
PDF is available here.
Brain Research (1231) 2008
Abstract
We studied the pattern of phosphorylated tau (labeled with AT8, CP13, PG5, and PHF1 antibodies) and plaques using immunohistochemical techniques. Phosphorylated tau-positive dystrophic neurites were exclusively associated with Congo red-positive plaques as previously reported. Further, we show that...
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PMID: 18606155
PDF is available here.
PDF is available here.
Abstract
We have investigated the impact of the disaccharide trehalose on the aggregation behavior of a model protein, bovine serum albumin (BSA) under moist conditions. About 50% aggregation of BSA was observed at a moisture level of 8 microl/10mg protein. Including trehalose in the protein sample caused a...
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PMID: 18407479
PDF is available here.
PDF is available here.
Abstract
We aimed to investigate the distribution of virulence genes in clinical isolates of pathogenic Yersinia enterocolitica. Two thousand six hundred stool samples were collected from 2600 patients with diarrhea, and were tested using the culture method and real-time PCR. Then, all isolates of pathogenic...
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PMID: 18557936
PDF is available here.
PDF is available here.
Biochemistry (Washington) (47)29 2008
Abstract
We show that the aggregation of a human antibody kappa-(kappa-MJM) and lambda-(lambda-L155) light chain (1 mg/mL) can be accelerated in vitro when they are incubated under physiologically relevant conditions, PBS, pH 7.4 and 37 degrees C, in the presence of a panel of biologically relevant lipid-der...
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PMID: 18578541
PDF is available here.
PDF is available here.
Abstract
Degradation of Congo Red (CR) dye in aqueous solutions was investigated by means of photocatalysis of TiO2 which was hydrothermally synthesized at 200 degrees C in 2 h, in anatase phase with 8 nm crystallite size. Efficiency of TiO2 in photocatalytic degradation under visible irradiation was studied...
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PMID: 18378395
PDF is available here.
PDF is available here.
Journal of Fish Diseases (31)7 2008
Abstract
Journal of Hazardous Materials (154)1-3 2008
Abstract
The adsorption of Congo Red onto bentonite in a batch adsorber has been studied. Four kinetic models, the pseudo first- and second-order equations, the Elovich equation and the intraparticle diffusion equation, were selected to follow the adsorption process. Kinetic parameters; rate constants, equil...
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PMID: 18055111
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1784)6 2008
Abstract
We report here that the single-domain globular protein, BCA II, is capable of forming amyloid fibrils. The primary sequence of BCA II was also analyzed using recurrence quantification analysis in order to suggest the probable residues responsible for amyloid formation....
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PMID: 18395531
PDF is available here.
PDF is available here.
Journal of Molecular Biology (379)1 2008
Abstract
We characterize bifurcation events accompanying insulin fibrillation upon intensive vortexing. Upon agitation, two types of insulin fibrils with opposite chiral senses are formed; however, predominance of either species is only stochastically determined. The uncertainty of fibrils' chiral sense hold...
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PMID: 18439622
PDF is available here.
PDF is available here.
Journal of Molecular Biology (378)5 2008
Abstract
We have used the SH3 domain of alpha-spectrin as a model globular protein in an attempt to understand the relationship between protein aggregation in the test-tube and in the more complex cellular environment. The investigation of the aggregation in Escherichia coli of this domain and a large set of...
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PMID: 18423663
PDF is available here.
PDF is available here.
FEBS Journal (275)9 2008
Abstract
As limited structural information is available on prion protein (PrP) misfolding and aggregation, a causative link between the specific (supra)molecular structure of PrP and transmissible spongiform encephalopathies remains to be elucidated. In this study, high pressure was utilized, as an approach...
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PMID: 18355314
PDF is available here.
PDF is available here.
Abstract
We present the detailed functional analysis of one of these MAPKs, MpkA. Comparative analysis revealed similarities of MpkA with MAPKs involved in cell wall integrity signaling of other fungi. Accordingly, the analysis of mpkA deletion mutants revealed severe sensitivity of the mutants against cell...
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PMID: 17981060
PDF is available here.
PDF is available here.
Abstract
We examined pancreatic tissue sections from HD patients at different disease stages. We found that the pattern of insulin immunostaining, levels of insulin transcripts and islet beta-cell area were similar in HD patients and controls. Further, there was no sign of amyloid deposition in islets from H...
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PMID: 18259770
PDF is available here.
PDF is available here.
Bioresource Technology (99)8 2008
Abstract
A continuous fixed bed study was carried out by using rice husk as a biosorbent for the removal of congo red (CR) from aqueous solution. The effects of important factors, such as the value of initial pH, existing salt, the flow rate, the influent concentration of CR and bed depth, were studied. Data...
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PMID: 17706420
PDF is available here.
PDF is available here.
Carbohydrate Research (343)5 2008
Abstract
A water-insoluble glucan, PFPSIN, has been isolated from the aqueous extract of an edible mushroom Pleurotus florida. On the basis of total acid hydrolysis, methylation analysis, periodate oxidation, Smith degradation, and (13)C NMR experiments, the repeating unit of the polysaccharide was establish...
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PMID: 18281022
PDF is available here.
PDF is available here.
Abstract
We developed a colorimetric and chromatographic assay for oseltamivir to assess the authenticity of Tamiflu (F. Hoffmann-La Roche Ltd., Basel, Switzerland) because of a growing concern about counterfeit oseltamivir. The colorimetric assay is quantitative and relies on an extractable colored ion-pair...
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PMID: 18394271
PDF is available here.
PDF is available here.
Abstract
We present the case of a 23-year-old woman with a parotid gland tumor, the fine-needle aspiration biopsy smears of which showed epithelial cells with wide cytoplasm, isolated or arranged in micropapillary groups together with psammoma bodies. The surgical specimen contained a 5-cm tumor with the his...
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PMID: 18417682
PDF is available here.
PDF is available here.
Journal of Neurology (255)4 2008
Abstract
FEBS Letters (582)6 2008
Abstract
We show that (trans,trans)-1-fluoro-2,5-bis(3-hydroxycarbonyl-4-hydroxy)styrylbenzene (FSB), a fluorescent Congo red derivative, labels tau inclusions in tissue sections from a mouse line transgenic for human P301S tau and in cases of familial frontotemporal dementia and sporadic Pick's disease. Lab...
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PMID: 18291106
PDF is available here.
PDF is available here.
Brain Research (1197) 2008
Abstract
We have investigated changes in the morphological structure of Abeta1-40 during different incubation time periods at 37 degrees C ranging from 1 h to 7 days using Thioflavin T, Congo red binding and electron microscopy. We found distinctive changes in Abeta assembly demonstrating the formation of be...
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PMID: 18237717
PDF is available here.
PDF is available here.
Abstract
Abstract
We report on the structural characterization of amyloid-like fibrils, self-assembled from synthetic polypentapeptides poly(ValGlyGlyLeuGly), whose monomeric sequence is a recurring, simple building block of elastin. This polymer adopts a beta-sheet structure as revealed by circular dichroism and Fou...
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PMID: 18257556
PDF is available here.
PDF is available here.
Bioresource Technology (99)5 2008
Abstract
N,O-carboxymethyl-chitosans (N,O-CMC) with different degree of substitution (DS) were synthesized under heterogeneous conditions by controlling the reaction temperature. The factors influencing adsorption capacity of N,O-CMC such as the DS of N,O-CMC, initial pH value of the dye solution and adsorpt...
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PMID: 17481892
PDF is available here.
PDF is available here.
Bioresource Technology (99)5 2008
Abstract
Aerated and rotated mode adsorption experiments were carried out for the removal of Congo red from aqueous solution using native and pre-treated mycelial pellets/biomass of Trametes versicolor. The effect of process parameters like contact time, dosage of adsorbent, adsorbate concentration and pH on...
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PMID: 17416520
PDF is available here.
PDF is available here.
Laboratory Investigation (88)3 2008
Abstract
Amyloid stained by Congo red is traditionally said to show apple-green birefringence in polarized light, although in practice various colors may be seen between accurately crossed polarizing filters, called polarizer and analyzer. Other colors are seen as the polarizer and analyzer are uncrossed and...
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PMID: 18166974
PDF is available here.
PDF is available here.
Abstract
Hybrid monolayers formed with an floating arachidic acid (AA) anions monolayer binding with a densely packed layered double hydroxides (LDHs) monolayer at an air/LDHs suspension interface has been studied by pi-A isotherms and TEM images. An ordered multilayer film of AA/LDHs has been fabricated by...
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PMID: 18036601
PDF is available here.
PDF is available here.
Molecular Vision (14) 2008
Abstract
HgammaD-crystallin, as well as its isolated NH2-terminal 1-82 and COOH-terminal 86-174 domains of HgammaD-crystallin formed amyloid fibrils upon incubation at acid pH. Investigations of early stages in cataract formation within the lens will be required to assess whether amyloid fibrils play a role...
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PMID: 18253099
PDF is available here.
PDF is available here.
Microbial Ecology (55)1 2008
Abstract
Flavobacterium spp. isolates have been identified in diverse biofilm structures, but the mechanism of adherence has not been elucidated. The absence of conventional biofilm-associated structures such as fimbriae, pili, and flagella suggest that surface hydrophobicity, and/or autoaggregation and coag...
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PMID: 17401596
PDF is available here.
PDF is available here.
Abstract
Under these conditions we observed atypical cystic, rolled and granular forms of these spirochetes. We characterized these abnormal forms by histochemical, immunohistochemical, dark field and atomic force microscopy (AFM) methods. The atypical and cystic forms found in the brains of three patients w...
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PMID: 18817547
PDF is available here.
PDF is available here.
Microbial Pathogenesis (44)1 2008
Abstract
We describe studies on the purification, structure, genetics and function of A. actinomycetemcomitans PGA. We found that PGA was very tightly attached to A. actinomycetemcomitans biofilm cells and could be efficiently separated from the cells only by phenol extraction. A. actinomycetemcomitans PGA c...
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PMID: 17851029
PDF is available here.
PDF is available here.
Annals of Medicine (40)3 2008
Abstract
The prevalence of SSA was 25%. SSA was associated with age, myocardial infarctions, the G/G (Val/Val) genotype of the exon 24 polymorphism in the alpha2-macroglobulin (alpha2M), and the H2 haplotype of the tau gene (P-values 0.002, 0.004, 0.042, and 0.016). CONCLUSION: This population-based study sh...
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PMID: 18382889
PDF is available here.
PDF is available here.