Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Trypan Blue (0):
Recent article
Trypan Blue
Abstract
We have described a method to improve the centration of donor trephination and reduce the incidence of eccentric donor punching....
|
PMID: 20847687
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Abstract
Sixty-four patients underwent macular hole surgery, and complete data were available on 49 patients (17 control subjects and 32 patients who had peeling of stained ILM). Trypan blue staining significantly improved ease and completeness of ILM removal. There was no significant difference in vision, o...
|
PMID: 19996832
PDF is available here.
PDF is available here.
Abstract
Optical coherence tomography assessment of the macular status at 6 months postoperatively showed closure of all macular holes and improvement in foveal contour in all cases of macular pucker or most cases of diabetic macular edema. Dissociated RNFL at the area of internal limiting membrane removal w...
|
PMID: 19952983
PDF is available here.
PDF is available here.
Abstract
Thirty-seven patients underwent ERM surgery, and 34 had complete data, of which 18 had peeling of unstained and 16 had peeling of stained ERM. Staining resulted in a significantly greater postoperative reduction in macular thickness compared with the unstained group. There was no significant differe...
|
PMID: 19996829
PDF is available here.
PDF is available here.
Abstract
Lysophosphatidylcholine (LPC) is a proinflammatory and proatherogenic substance, and it plays an important role in the initiation, progression, and destabilization of atherosclerotic plaques. If LPC in the vascular wall is visualized in vivo, the mechanisms of atherosclerosis and the effects of medi...
|
PMID: 20379048
PDF is available here.
PDF is available here.
Abstract
Three months after surgery there was a significant difference in increase in visual field in the superior region in favor of the membrane blue group (p = 0.021). Eight eyes (53%) of the infracyanine group had a decrease in temporal visual fields of at least 5 degrees . CONCLUSION: Although there was...
|
PMID: 19823863
PDF is available here.
PDF is available here.
Abstract
We have consistently observed an inversely proportional relationship between UC attachment efficiency and the amount of extraneous materials in the suspension; viable UC cell attachment efficiency decreases as the amount of extraneous materials in the cell suspension increases. Processing the initia...
|
PMID: 19351240
PDF is available here.
PDF is available here.
Abstract
A prototype ocular mini drug pump was built, implanted, and refilled. Such a platform needs more testing to determine the long-term biocompatibility of an electrically controlled implanted pump. Testing with various pharmacologic agents is needed to determine its ultimate potential for ophthalmic us...
|
PMID: 20126483
PDF is available here.
PDF is available here.
Molecular Endocrinology (23)8 2009
Abstract
We previously reported that miR-206 and ERalpha repress each other's expression in MCF-7 cells in a double-negative feedback loop. We show herein that miR-206 coordinately targets mRNAs encoding the coactivator proteins steroid receptor coactivator (SRC)-1 and SRC-3, and the transcription factor GAT...
|
PMID: 19423651
PDF is available here.
PDF is available here.
Abstract
The use of trypan blue during small-incision cataract surgery does not have any effect on IOP during the immediate and early postoperative period....
|
PMID: 19491985
PDF is available here.
PDF is available here.
Abstract
At a concentration of 0.5 mg/ml, CHX and, to a lesser extent, MINO had a cytotoxic effect on osteoblast proliferation in vitro. However, DOXY seemed to enhance maturation and differentiation rather than proliferation. In addition to DOXY's beneficial effect as an adjunctive therapy to mechanical deb...
|
PMID: 19485832
PDF is available here.
PDF is available here.
Abstract
HSO may be used as tamponade in patients with unsuccessful primary macular-hole surgery, negating the need of prone posturing post-operatively....
|
PMID: 18972124
PDF is available here.
PDF is available here.
Biotechnology Letters (31)4 2009
Abstract
Fifty-four strains of lactic acid bacteria obtained from fermented dairy milks were investigated for possible use as probiotics and for colon cancer biological products. Five of these strains inhibited growth of eight food-borne pathogens including Helicobacter pylori, Escherichia coli, and Salmonel...
|
PMID: 19116692
PDF is available here.
PDF is available here.
Abstract
A rapid staining procedure for detection of recent skin and fin injuries was tested in juvenile Chinook salmon Oncorhynchus tshawytscha. Immersion of anesthetized fish for 1 min in aerated aqueous solutions of the synthetic food dye fast green FCF (Food Green 3) at concentrations of 0.1 to 0.5% prod...
|
PMID: 19476284
PDF is available here.
PDF is available here.
Abstract
Our results indicate an inadequate osteoblastic function in osteoporosis and demostrate that 1,25-dihydroxy-vitamin D(3) can stimulate the metabolic activity of human osteoblasts in vitro. Considering that osteoporotic bone samples were representative of senile osteoporosis, our results may indicate...
|
PMID: 18989673
PDF is available here.
PDF is available here.
Abstract
Results suggest that Dye Extrusion Technique may be an effective technique for the identification of occult retinal breaks and may facilitate simple techniques of detachment repair. No evidence of retino-toxicity from the use of trypan blue was found but the study had limited power to detect adverse...
|
PMID: 19262427
PDF is available here.
PDF is available here.
Abstract
After 1-h elution only the highest dose of Diaket, Hermetic and SuperEBA significantly (P = 0.035, P = 0.048, P = 0.037 respectively) affected the measured cytogenetic parameters. The migration ability of DNA was more strongly affected than induction of chromosomal aberrations. After elutions longer...
|
PMID: 19228216
PDF is available here.
PDF is available here.
Abstract
Shear forces and free radicals produced by acoustic cavitation may play important roles in these actions....
|
PMID: 19243247
PDF is available here.
PDF is available here.
Experimental Parasitology (121)2 2009
Abstract
We established a system to screen drugs in vitro that involved using cell viability methods to calculate drug selectivities, which are Trypan blue, [3-(4,5-dimethylthiazol-zyl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazoliuzolium, inner salt] (MTS) method and lactate dehydrogenase (LDH)...
|
PMID: 18977350
PDF is available here.
PDF is available here.
Abstract
The use of trypan blue led to significantly stiffer anterior lens capsules (P= .036). Trypan blue had the greatest effect on the stiffness of diabetic lens capsules (P= .046). CONCLUSION: Trypan blue contributed to increased capsule stiffness during capsulorhexis, especially in diabetic patients....
|
PMID: 19185249
PDF is available here.
PDF is available here.
Abstract
We report that classical dye-based assays such as MTT and neutral red (NR) that determine cell viability produce invalid results with some NM (nanomaterials) due to NM/dye interactions and/or NM adsorption of the dye/dye products. In this study, human epidermal keratinocytes (HEK) were exposed in vi...
|
PMID: 18983864
PDF is available here.
PDF is available here.
Modern Rheumatology (19)4 2009
Abstract
We report here our investigation of whether geranylgeranylacetone (GGA) induces cell death in RA synoviocytes. Synovial tissues were obtained from patients with RA at the time of total knee arthroplasty. Fibroblast-like synoviocytes (FLS) cultured in three passages were used for the experiments. The...
|
PMID: 19526306
PDF is available here.
PDF is available here.
Abstract
We report a case of iris atrophy and corneal endothelial decompensation following the inadvertent use of water-based ink for capsular staining during cataract surgery. The anterior chamber was thoroughly irrigated with balanced salt solution after the identification of the wrong dye. Phacoemulsifica...
|
PMID: 19888890
PDF is available here.
PDF is available here.
Abstract
Twenty-four macular holes were studied. The ILMs were selectively stained by trypan blue and then removed completely. No visible dye remained on the retina after peeling. The macular holes were closed in 22 eyes (92%) after a single surgery. The mean best-corrected visual acuity improved from 20/140...
|
PMID: 19694607
PDF is available here.
PDF is available here.
Abstract
Ultrasound-mediated gene transfection (sonotransfection) has been shown to be a promising physical method for gene therapy, especially for cancer gene therapy. The procedure being done in vitro uses several ultrasound exposure (sonication) setups. Although high transfection rates have been attained...
|
PMID: 19565903
PDF is available here.
PDF is available here.
Abstract
These results suggest that estrogen deficiency induces a loss of barrier function and an increase in hypoxia. The estrogen-dependent decreases of prostaglandin PGE(2) in the urothelium correlate with loss of barrier function, suggesting estrogen regulation of PGE(2) may contribute to maintenance of...
|
PMID: 19181506
PDF is available here.
PDF is available here.
Abstract
Manual small incision cataract surgery (MSICS) involves the manual removal of nucleus through a scleral tunnel. To achieve 100% success every time, one has to do a good capsulotomy and should master the technique to prolapse the nucleus into anterior chamber. During conversion from extracapsular cat...
|
PMID: 19075402
PDF is available here.
PDF is available here.
Abstract
We report in this study that 2Am-DNT treatment of mammalian cells causes cell death by apoptosis. Cell death was assayed by the Trypan Blue method. Apoptotic changes, such as DNA break down, were detected in treated cells by the production of a dark-brown DAB (diaminobenzidine) signal using the Frag...
|
PMID: 19888910
PDF is available here.
PDF is available here.
Abstract
We compare three methods for cell viability (CV) determination: (i) lactate dehydrogenase (LDH) staining on cryosections, (ii) calcein AM/ethidium homodimer-1 (CaAM/EthH) staining, and (iii) carrier digestion and trypan blue (TB) assay. Living and dead cell populations were generated from bovine cho...
|
PMID: 18800876
PDF is available here.
PDF is available here.
Abstract
Promoted VSMC proliferation significantly increased the number of VSMCs in the S phase, the expressions of PCNA and MMP-2, and MMP-2 activity, as well as decreased the proportion of VSMCs in the G(0)/G(1) phase. Rosiglitazone at a concentration of 10 mumol/L markedly inhibited glucose-induced VSMC p...
|
PMID: 18665442
PDF is available here.
PDF is available here.
Abstract
These results indicate that one-step dentin-bonding agents differ markedly in their cytotoxicity. Differential cytotoxic effects of one-step dentin-bonding agents should be considered during clinical application of operative restoration....
|
PMID: 18976265
PDF is available here.
PDF is available here.
Abstract
The results clearly indicate that the Lipo-Ga-GEN formulation is more effective than gentamicin alone in eradicating antibiotic-resistant P. aeruginosa isolates growing in a planktonic or biofilm community....
|
PMID: 18931388
PDF is available here.
PDF is available here.
Clinica Chimica Acta (397)1-2 2008
Abstract
Formaldehyde addition showed no effect on the percentage of fetal DNA in samples processed 6 h after blood collection. In samples processed at 36 h, formaldehyde addition inhibited blood cell lysis and nuclease-mediated DNA degradation, thus markedly decreasing the concentration of total DNA and inc...
|
PMID: 18692490
PDF is available here.
PDF is available here.
Abstract
This paper reports on a semi-automated microrobotic system for adherent cell injection. Different from embryos/oocytes that have a spherical shape and regular morphology, adherent cells are flat with a thickness of a few micrometers and are highly irregular in morphology. Based on computer vision mi...
|
PMID: 19044735
PDF is available here.
PDF is available here.
Archives of Dermatology (144)9 2008
Abstract
Abstract
Uniform gelatin microspheres (GMS) of a wet size of 100 microm in diameter were fabricated by the electric field assisted precision particle fabrication (E-PPF) method and crosslinked with different glutaraldehyde (GA) concentrations to study the effect of the crosslinking density on drug release. T...
|
PMID: 18446808
PDF is available here.
PDF is available here.
Abstract
We now have found that these lactams also possess antifungal activity against Candida and other fungi by exerting powerful cytostatic effects that disrupt the structural integrity of cytoplasmic membranes. The mode of action and structure-activity trends of these lactams as antifungals parallel that...
|
PMID: 18672374
PDF is available here.
PDF is available here.
Abstract
This study was conducted for morphological and quantitative evaluation of the diffusional pattern of infiltration anesthesia according to the site of injection. The maxillary first molar of rats was anesthetized by local infiltration of 100 microL of 2% lidocaine containing trypan blue at a distance...
|
PMID: 18554955
PDF is available here.
PDF is available here.
Dental Materials (24)8 2008
Abstract
Blue light exposure significantly inhibited proliferation and SDH activity of HGF in a dose-dependent manner; exposure more than 12J/cm(2) had a toxic effect on the cells. The blue light-induced cytotoxicity of the cells resulted from apoptosis, as proven by the migration of many cells to the sub-G(...
|
PMID: 18164755
PDF is available here.
PDF is available here.
Abstract
The aim of this study was to determine the toxic effect of atrazine at the ovarian cellular level. Chinese Hamster Ovary (CHO-K1) cell line was used to evaluate the degree of in vitro atrazine cytotoxicity and the morphological changes were followed during the cell death. Application of four bioassa...
|
PMID: 18465069
PDF is available here.
PDF is available here.
Archives of Dermatology (144)8 2008
Abstract
Abstract
Vital dye staining of corneal endothelial cells can be combined with Adobe Photoshop software to yield a quantitative assessment of areas of acute endothelial cell loss and/or damage. This described technique holds promise for a more consistent and accurate method to evaluate the surgical trauma to...
|
PMID: 18650669
PDF is available here.
PDF is available here.
Abstract
This study shows that endothelial damage occurs in ALCT-off corneas. We hypothesize that the absence of the Bowman layer may contribute to the damage, because it has been shown that the Bowman layer provides a barrier function. These data suggest that it is important to keep the ALCT/Bowman layer on...
|
PMID: 18650658
PDF is available here.
PDF is available here.
Abstract
Trypan blue staining affected the biomechanical properties of the human lens capsule and led to a significant reduction in elasticity and an increase in stiffness. These results confirm clinical reports of changes in the capsule by dye and should be taken into consideration in dye-enhanced cataract...
|
PMID: 18655989
PDF is available here.
PDF is available here.
Abstract
We describe a technique that uses trypan blue dye to identify residual recipient corneal stroma and Descemet membrane (DM) during conversion of deep anterior lamellar keratoplasty (DALK) to penetrating keratoplasty (PKP). After the host cornea is dissected, trypan blue dye (0.06%) is used to highlig...
|
PMID: 18655971
PDF is available here.
PDF is available here.
Abstract
We assessed the effect of a neuropeptide, bombesin, on keratinocytes during regeneration from scratch wounding. Bombesin purified from amphibian skin, is homologous of mammalian gastrin-releasing peptide and is active in mammals. Its pharmacological effects mediate various physiological activities:...
|
PMID: 18455266
PDF is available here.
PDF is available here.
Toxicology Letters (179)1 2008
Abstract
We have previously proposed that modulation of intracellular calcium ([Ca2+]i) homeostasis induced by As2O3 could be an important mechanism to induce cytotoxicity. Here we demonstrate, using human cell models (neuroblastoma (SY-5Y) or embryonic kidney cells (HEK)) and confocal microscopy in combinat...
|
PMID: 18485628
PDF is available here.
PDF is available here.
Molecular Biotechnology (39)2 2008
Abstract
We found no evidence of long-term protection of cells once Pluronic was removed. Pluronic was capable of repairing trypsin-damaged cells as evidenced by enhanced growth, reduced membrane porosity, and improved robustness under liquid shear. The proportion of adherent cells was reduced to a minimal l...
|
PMID: 18327558
PDF is available here.
PDF is available here.
Cryobiology (56)3 2008
Abstract
We reported some preliminary results on cryopreservation of zebrafish (Danio rerio) oocytes using controlled slow cooling and determined the optimum cryoprotective medium and cooling rate for stage III zebrafish oocytes. In the present study, the effects of two different cryopreservation media, cryo...
|
PMID: 18433743
PDF is available here.
PDF is available here.
Abstract
Abstract
We suggest that, before a commercial TA solution is used intravitreally, the vehicle should be removed to prevent damaging the RPE layer, particularly during macular hole surgery. Commercial development of a preservative-free TA suspension for intraocular use is urged....
|
PMID: 18420195
PDF is available here.
PDF is available here.
Abstract
Copper, an essential trace element, induces apoptosis in mammalian cells. However, the precise mechanism of copper-induced apoptosis is still unclear. In this study, to determine the apoptotic pathway initiated by copper treatment, apoptotic factors such as Bax, Bad and Bcl-2, and the caspase family...
|
PMID: 18381230
PDF is available here.
PDF is available here.
Abstract
Marking the DSAEK donor stromal surface with a gentian violet marker damages the endothelium. Surgeons should limit the size of the mark or use an insertion technique that avoids confusion about orientation of the donor cornea....
|
PMID: 18520506
PDF is available here.
PDF is available here.
Abstract
At time point 1, a statistically significant difference was observed in the ingress of trypan blue in the aqueous aspirate. The bimanual group had the highest ingress and the microcoaxial group, the lowest. The amount of ingress in the standard coaxial group fell between the other 2 groups (P< .001)...
|
PMID: 18499010
PDF is available here.
PDF is available here.
Abstract
ICG is decomposed by light within a self-sensitized photo oxidation. The decomposition products reduce the viability of RPE cells in vitro. The toxic effects of decomposed ICG should be further investigated under in vivo conditions....
|
PMID: 18436812
PDF is available here.
PDF is available here.
Abstract
In the present case series, PPV with Trypan Blue-assisted ILM peeling, short-acting internal tamponade and thus shorter face-down positioning was associated with a 93% macular hole closure rate....
|
PMID: 18296507
PDF is available here.
PDF is available here.
Abstract
We investigated the anti-tumor effect of C. versicolor methanol extract (which contains terpenoids and polyphenols) on B16 mouse melanoma cells both in vitro and in vivo.In vitro treatment of the cells with the methanol extract (25-1600 microg/ml) reduced melanoma cell viability in a dose-dependent...
|
PMID: 18313195
PDF is available here.
PDF is available here.
Abstract
The infection of foetal thymus with coxsackievirus B4 (CV-B4) E2 has been studied ex vivo by using CD-1 mice on foetal day 14, as a ready source of organs for experimentation to investigate the hypothesis of the role of thymic viral infections in the pathogenesis of type 1 diabetes. The replication...
|
PMID: 18297721
PDF is available here.
PDF is available here.
Abstract
On post-op day 1, 18% had an increased cellular response in the anterior chamber and anterior vitreous precluding fundus visualization, 12% had a hypopyon. Initial visual acuity (VA) ranged from hand motions to light perception. Eyes were initially managed with topical and oral steroids. Two eyes un...
|
PMID: 17634862
PDF is available here.
PDF is available here.
Anti-Cancer Drugs (19)3 2008
Abstract
We assessed the apoptotic effect of DHA on leukemia HL60 cells and its regulation of transferrin receptor (TfR). Cell growth inhibition was assessed by Trypan blue exclusive staining; the expression of caspase-3, Bcl-2, and Bax in HL60 cells was evaluated by Western blotting; DHA-induced apoptosis w...
|
PMID: 18510170
PDF is available here.
PDF is available here.