Abstract
A new high-sensitivity detection of protein assay at the nanogram level is proposed based on multi-spectroscopic methods including resonance light scattering (RLS), atomic force microscopy (AFM), ultraviolet spectra (UV) and fluorescence spectra etc. Under the optimum conditions, the amplified RLS s...
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PMID: 20638325
PDF is available here.
Abstract
Results indicated that AV7 showed a significant ability to induce chromosome aberrations, lipid peroxidation, and acetylcholinesterase inhibitory effect. The toxicity of AV7 increased significantly after static biodegradation with P. putida mt-2 and totally disappeared after shaken incubation. In ad...
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PMID: 20369386
PDF is available here.
Abstract
Ce-doped titania hollow spheres were prepared using carbon spheres as template and Ce-doped titania nanoparticles as building blocks. The Ce-doped titania nanoparticles were synthesized at low temperature. The prepared hollow spheres were characterized by X-ray diffraction (XRD), transmission electr...
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PMID: 20149535
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Anatoli Kamali,
Helen Byomire,
Catherine Muwonge,
Julie Bakobaki,
Clare Rutterford,
Pius Okong,
Albert Profy,
Romano Byaruhanga,
Stella Namukwaya,
Sheena McCormack,
Heiner Grosskurth,
Andrew J Nunn and
Charles J N Lacey
Abstract
Both concentrations of PRO 2000 gel were found to be safe and well tolerated. These data justified testing the gels in large-scale effectiveness trials....
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PMID: 20444744
PDF is available here.
Motoki Goto,
Kayo Shimada,
Ayako Sato,
Eri Takahashi,
Takafumi Fukasawa,
Tomoki Takahashi,
Seii Ohka,
Takahide Taniguchi,
Eiichi Honda,
Akio Nomoto,
Atsuo Ogura,
Teruo Kirikae and
Ken-Ichi Hanaki
Abstract
A colorimetric loop-mediated isothermal amplification (LAMP) assay with hydroxy naphthol blue was designed to amplify a region in the outer membrane lipoprotein (oprL) gene of Pseudomonas aeruginosa. The LAMP assay showed 100% specificity for the serogroup and other bacteria, and the sensitivity was...
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PMID: 20298724
PDF is available here.
Abstract
As new pharmaceutical products to combat the acquisition of HIV are produced, their clinical efficacy is determined through large-scale clinical trials. Trial participants, however, also independently evaluate the effectiveness of these technologies. During a phase III microbicide clinical trial in...
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PMID: 20419520
PDF is available here.
Abstract
Besides cardiovascular diseases, cancer represents the major cause of death in developed countries. In many different human tumors, increased activity of serine/threonine protein kinase CK2 has been detected, and recent in vivo studies support a direct involvement of CK2 in tumor progression. Theref...
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PMID: 19874207
PDF is available here.
Abstract
We concluded that this colorimetric RT-LAMP assay had potential for the rapid screening of the human influenza A H1N1 virus infection in National influenza monitoring network laboratories and sentinel hospitals of provincial and municipal region in China....
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PMID: 20480635
PDF is available here.
Abstract
We therefore examined whether these growth factors could activate m-calpain by MAPK-dependent phosphorylation using cultured primary neurons and HEK-TrkB cells, both of which express BDNF and EGF receptors. Calpain activation was monitored by quantitative analysis of spectrin degradation and by a fl...
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PMID: 20089917
PDF is available here.
Abstract
In the present study, Fenton's oxidation of a chromium complex disazo dye (Acid Blue 193) synthesis wastewater was evaluated, modeled and optimized by employing Central Composite Design. Within this context, the individual and interactive effects of critical process parameters such as Fe(2 + ), H(2)...
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PMID: 20595773
PDF is available here.
Abstract
An activated carbon-MnO(2) catalyst was prepared by the dipping-calcination method using activated carbon as catalyst support. The catalyst was used for the catalytic oxidation of simulated acid chrome blue K wastewater. The COD removal efficiency and decolor efficiency by catalytic oxidation are 72...
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PMID: 20388989
PDF is available here.
Abstract
Wastewaters of textile and leather dying industries may contain significant quantities of chromium(VI) ions besides anionic and water-soluble dyes. Moreover the temperature of these wastewaters may be a controlling parameter affecting the biosorption efficiency. In this study biosorp...
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PMID: 19278783
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Abstract
Uvitex 2B detected 5/120 (4.2%) while Chromotrope 2R detected 4/120 (3.3%) and there was no statistical significance difference between the two methods (P>0.05). The sensitivity and specificity of the chromotrope-2R method relative to Uvitex-2B were 80% and 100%, respectively and positive and negati...
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PMID: 19954127
PDF is available here.
Abstract
Loop-mediated isothermal amplification (LAMP), a novel gene amplification method, enables the synthesis of larger amounts of both DNA and a visible byproduct--namely, magnesium pyrophosphate--without thermal cycling. A positive reaction is indicated by the turbidity of the reaction solution or the c...
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PMID: 19317660
PDF is available here.
Abstract
Cultured glans and foreskin showed comparable epithelial thickness but some differences in CD4 and CD1a cell density. All tissue sites examined (foreskin, glans, meatus, urethra) were equally susceptible to R5 HIV-1 infection, which was productively disseminated by migratory cells emigrating from ti...
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PMID: 19114867
PDF is available here.
Abstract
Orientation and mobility of fluorescent probe N-((iodoacetyl)-(1-naphtyl-5-sulpho-ethylenediamine)(1.5-IAEDANS)) specifically bound to Cys-374 of actin in ghost muscle fibers isolated from fast and slow rat muscles were studied by polarized fluorimetry in the absence and presence of myosin subfragme...
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PMID: 19799356
PDF is available here.
Abstract
These findings indicate that disruption of the epithelium contributes to increased HSV-2 susceptibility and might provide a biomarker predictive of increased risk for HIV acquisition. The results are consistent with the safety outcomes of the recently completed Phase IIb clinical trial with 0.5% PRO...
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PMID: 20032541
PDF is available here.
Abstract
In this work, an upconversion luminescence agent, crystallized Er(3 + ):YAlO(3), was synthesized and then coated by the nano-sized TiO(2) film through sol-gel technique. A novel TiO(2) photocatalyst, Er(3 + ):YAlO(3)/TiO(2), with high activity in visible light was subsequently prepared. The Er(3 + )...
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PMID: 19700830
PDF is available here.
Abstract
The paper summarises the definition of an extended biokinetic model dedicated to micropollutant degradation in wastewater treatment and the parameter estimation methodology for this model. Additionally it describes results on experimental parameter estimation for two target micropollutants, naphthal...
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PMID: 19151497
PDF is available here.
Abstract
In the crystal structure of the complex between the soluble extracellular domain of tissue factor (sTF) and active-site-inhibited VIIa, residues 91 and 92 in the Pro(79)-Pro(92) loop of sTF interact with the catalytic domain of VIIa. It is not known, however, whether this loop has a role in alloster...
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PMID: 19167313
PDF is available here.
Abstract
This study focused on the effects of decolorization products of reactive black 5 (RB5) on anaerobic decolorization of direct yellow 11 (DY11) by Rhodopseudomonas palustris W1 and its reaction mechanisms. The results showed that the decolorization products of RB5 could obviously accelerate biological...
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PMID: 19186827
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Abstract
We have found that this lectin also interacts with adenine (k(D)=5.4+/-0.5 microM) and arylaminonaphthalene sulfonate TNS (k(D)=12+/-0.3 microM). Binding of HPA to hormones and adenine was accompanied by a significant increase of the intrinsic Trp fluorescence (up to 50%), characterizing the conform...
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PMID: 18501393
PDF is available here.
Abstract
PEG-CCK9 dose-dependently induced CTA, with a minimal effective dose of 8 microg kg(-1), whereas the minimal effective dose to induce satiety was 1 microg kg(-1). The CTA effects of PEG-CCK9 were completely abolished by i.p. administration of devazepide prior to PEG-CCK9 treatment and only partially...
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PMID: 18574458
PDF is available here.
Abstract
As a family of hydrophobic ionizable organic compounds, aromatic sulfonates can be present at high levels in industrial wastewaters. They tend to exist as anions over a wide range of pH and cannot be effectively trapped by conventional adsorbents. In the current study, a recyclable acrylic ester pol...
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PMID: 18939579
PDF is available here.
Abstract
An efficient route to prepare ionic liquids with chloroalkylsulfonate anions is presented; the synthesis proceeds in a one-step ring-opening reaction of sultones with an organic chloride salt and provides a very attractive access to new anion functionalised ionic liquids.
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PMID: 18726017
PDF is available here.
Abstract
These results and resonance light scattering experiment suggest that the binding force between TNS and Cen is hydrophobic. The distance (r) between TNS and tryptophan of mutant G115W, which sheds more insight into the binding of TNS to Cen, was determined as 4.85nm based on Förster non-radiative en...
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PMID: 18054271
PDF is available here.
Abstract
Time-resolved vibrational femtosecond spectroscopy is employed to investigate the photoinduced Wolff rearrangement reaction of diazonaphthoquinone (DNQ) dissolved in different solvents (methanol and water). DNQ is an important compound in commercial Novolak photoresists. Upon photoexcitation the ket...
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PMID: 19044874
PDF is available here.
Abstract
The white-rot fungus Bjerkandera sp. BOL-13 was evaluated regarding decolorization of four textile dyes Reactive blue 21, Reactive black 5, Reactive orange 13 and Reactive yellow 206. Experiments were performed in batch and continuous modes. The total dye concentration in all experiments was 100 mg...
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PMID: 18724647
PDF is available here.
Abstract
Among the many applications of 4-hydroxy-1-naphthalenesulfonic acid sodium salt (HNSA, CAS No. 6099-57-6) is its use as a common intermediate in the manufacture of dyes used as color additives. In order to develop an HPLC method to analyze HNSA in such additives, it was necessary to obtain pure HNSA...
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PMID: 18541251
PDF is available here.
Abstract
SiO2-C2H4-ferrocene (SiCFe) was synthesized by covalent grafting of ferrocene on functionalized silica gel with a -C2H4- linkage. On the basis of characterization by diffuse reflectance UV-vis spectra (DRS) and Fourier transform infrared spectra (FT-IR), ferrocene has been successfully anchored on t...
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PMID: 18023969
PDF is available here.
Abstract
This study makes a comparison between photo-Fenton and a novel electro-Fenton called Fered-Fenton to study the mineralization of 10,000 mg/L of dye-Reactive Black B (RBB) aqueous solution, which was chosen as the model dye contaminant. Results indicate that the traditional Fenton process only yields...
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PMID: 18063472
PDF is available here.
Abstract
This study elucidates the decolorization of C.I. Reactive Red 2 (RR2) by homogeneous catalytic ozonation. The effects of pH and catalyst dosage were evaluated in O3/Mn(II), O3/Fe(II), O3/Fe(III), O3/Zn(II), O3/Co(II) and O3/Ni(II) systems. In O3/Mn(II), O3/Fe(II) and O3/Fe(III) systems, increasing t...
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PMID: 18068896
PDF is available here.
Abstract
A new method for the highly sensitive and selective determination of boron at nanograms per cubic decimeter levels has been developed based on the derivatization reaction of boron using salicylaldehyde and 1-amino-8-naphtol-3,6-disulfonate with reversed-phase partition high-performance liquid chroma...
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PMID: 18415086
PDF is available here.
Abstract
We report detailed investigations into the interactions of Ga(3+) with apoovotransferrin at the molecular level. First, the characteristics of Ga(3+) binding to apoovotransferrin were analyzed using UV difference spectra. The results show that Ga(3+) prefers to bind to the N-terminal site rather tha...
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PMID: 18430582
PDF is available here.
Abstract
The chromium(III) complex species formed, in acidic and basic solutions at 25.0+/-0.1 degrees C, between Cr(III) and 2,3-dihydroxynaphthalene-6-sulfonic acid (2,3-DHN-6-SA, H(2)L(2-)) and 4,5-dihydroxynaphthalene-2,7-disulfonic acid (4,5-DHN-2,7-DSA, H(2)L(-)) were determined. Over the acidic pH ran...
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PMID: 18520078
PDF is available here.
Abstract
A study was undertaken to evaluate the efficiency of adsorption and desorption of three reactive dyes on to modified chitin. Black DN from the chlorotriazine group, Scarlet R from the vinylsulfone group of dyes and a chlorovinyl dye, Brilliantorange 3R, were examined. Following adsorption of the dye...
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PMID: 18702294
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Abstract
We show that this pretreatment can overcome the color interference and lead to highly accurate and precise boron analyses in wastewater. In soil extracts, the boron concentrations obtained using the suggested procedure were better correlated to inductively coupled plasma (ICP) spectrometry results t...
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PMID: 18396315
PDF is available here.
Abstract
This study discusses the effects of ultrasound (US) irradiation on the decolorization of C.I. Reactive Red 198 (RR198) in UV/ZnO system. The influences of ZnO dosage, pH and the addition of NaCl or a radical scavenger were evaluated. The decolorization rate of RR198 increased with the ZnO dosage in...
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PMID: 17980480
PDF is available here.
Abstract
These results indicate that biomass fly ash is an interesting alternative for dye removal from the wastewater....
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PMID: 17981394
PDF is available here.
Abstract
This study adopted O3, UV/TiO2/O3, O3/Mn(II) and O3/MnO2 systems to assess the decolorization efficiency of C.I. Reactive Red 2 (RR2). The decolorization rate increased with concentrations of Mn(II) and MnO2 in the ranges 0.05-0.1 and 0.05-0.8 g/l, respectively. However, when 0.5-3g/l TiO2 was added...
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PMID: 17964715
PDF is available here.
Abstract
We have previously cloned and sequenced the neocarzinostatin biosynthetic gene cluster and proposed that the biosynthesis of the naphthoic acid moiety and its incorporation into the neocarzinostatin chromophore are catalyzed by five enzymes NcsB, NcsB1, NcsB2, NcsB3, and NcsB4. Here we report the bi...
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PMID: 18387946
PDF is available here.
Abstract
The combined electrochemical oxidation-solar-light/immobilized TiO2 film process was conducted to degrade an azo dye, Reactive Black 5 (RB5). The toxicity was also monitored by the Vibrio fischeri light inhibition test. The electrochemical oxidation rapidly decolorized RB5 (55, 110 microM) with a su...
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PMID: 18359062
PDF is available here.
Carol Lackman-Smith,
Clay Osterling,
Katherine Luckenbaugh,
Marie Mankowski,
Beth Snyder,
Gareth Lewis,
Jeremy Paull,
Albert Profy,
Roger G Ptak,
Robert W Buckheit,
Karen M Watson,
James E Cummins and
Brigitte E Sanders-Beer
Abstract
Topical microbicides are self-administered, prophylactic products for protection against sexually transmitted pathogens. A large number of compounds with known anti-human immunodeficiency virus type 1 (HIV-1) inhibitory activity have been proposed as candidate topical microbicides. To identify poten...
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PMID: 18316528
PDF is available here.
Abstract
Without an effective vaccine against human immunodeficiency virus (HIV) infection, topical microbicide development has become a priority. The sulfonated polyanion PRO 2000, a candidate topical microbicide now in phase II/III clinical trials, blocks HIV infection of cervical tissue in vitro. Dendriti...
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PMID: 18332174
PDF is available here.
Abstract
The photocatalytic degradation of C.I. Direct Red 23 (4BS) in aqueous solutions under UV irradiation was investigated with SrTiO3/CeO2 composite as the catalyst. The SrTiO3/CeO2 powders had more photocatalytic activity for decolorization of 4BS than that of pure SrTiO3 powder under UV irradiation. T...
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PMID: 17850958
PDF is available here.
Abstract
Removal of Reactive Black 5 (RB5) from aqueous solutions was carried by the sorption process in the presence and in the absence of ultrasound. Sorption of the dye on the solid phase was investigated in a series of batch sorption experiments to determine the influence of different parameters such as...
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PMID: 17962064
PDF is available here.
Abstract
In this work, Fe-doped mixed crystal TiO2 powder as sonocatalyst was prepared by the sol-gel method and heat treatment, and a novel method combined with ultrasonic irradiation was propounded to degrade the organic polluted water. First, the Fe-doped mixed crystal TiO2 powder was characterized by TG-...
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PMID: 18206158
PDF is available here.
Abstract
We have explored binding interaction of a ligand protoporphyrin IX (PPIX), which is demonstrated (X-ray crystallography) to reside in domain-IB at the various pH-induced folded states of HSA. The ligand PPIX is found to remain attached to all the HSA conformers which offers an opportunity to use Fö...
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PMID: 18276152
PDF is available here.
Abstract
This study investigated the decolorization efficiency of C.I. Reactive Red 2 (RR2) in O3, O3/H2O2, O3/Fe3+, O3/H2O2/Fe3+, UV/O3, UV/O3/Fe3+, UV/O3/H2O2 and UV/O3/H2O2/Fe3+ systems at various pHs. The effective energy consumption constants and the electrical energy per order of pollutant removal (EE/...
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PMID: 17683857
PDF is available here.
Abstract
Polymer membrane electrodes operated by pulsed chronopotentiometry have recently been introduced to replace traditional ion-selective electrodes for a number of applications. While ion-selective electrodes for the polycation protamine have been reported, for instance, a pulsed chronopotentiometric r...
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PMID: 18022377
PDF is available here.
Abstract
Adsorption kinetic and equilibrium studies of three reactive dyes namely, Remazol Brillant Blue (RB), Remazol Red 133 (RR) and Rifacion Yellow HED (RY) from aqueous solutions at various initial dye concentration (100-500 mg/l), pH (2-8), particle size (45-112.5 microm) and temperature (293-323 K) on...
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PMID: 17574338
PDF is available here.
Abstract
We initiated a physicochemical study of the self-assembly properties of the purified protein in vitro. The native PAGE results indicate that only the integral protein shows multimers in the presence of Lu(3+). The dependence of Lu(3+) induced self-assembly of EoCen on various chemical and physical f...
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PMID: 17935787
PDF is available here.
Abstract
Indevus Pharmaceuticals Inc, under license from Paligent Inc, is developing PRO-2000, an antimicrobial gel for the prevention of HIV infection. The company is also investigating its potential to prevent the transmission of other sexually transmitted diseases. In February 2005, Indevus launched a piv...
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PMID: 18246522
PDF is available here.
Abstract
The interaction of Acid chrome blue K (ACBK) with nucleic acids in weak basic medium was studied in the presence of cetyltrimethylammonium bromide (CTMAB) based on the measurements of resonance light scattering (RLS), UV-vis, NMR spectra, etc. In hexamethylene tetramine (HMTA) buffer (pH 7.45), ACBK...
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PMID: 17611144
PDF is available here.
Abstract
Reactive black 5 (RB5), an azo dye, was degraded by using UV-irradiated TiO(2) coated on non woven paper with SiO(2) as a binder. The adsorption capacity of the photocatalyst was studied at natural pH, superior to pH(pzc) of the binder, for various ionic strengths. Different salts such as NaCl, KCl,...
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PMID: 17543449
PDF is available here.
Abstract
Colour in food is an integral part of our culture and is also indispensable to the modern day consumer. During the past several decades, the technology of food processing has changed dramatically and the growth in the use of synthetic food colours as an individual dye or in the form of blends has inc...
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PMID: 18222076
PDF is available here.