Find your PDFs fast.
Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Membrane Potentials (0):
Recent article
Membrane Potentials
Abstract
We monitored the assembly and localization of cell surface channel complexes in living cells. While PSD95-mediated clustering was subunit independent, selective visualization of heteromeric Kv complexes in rat hippocampal neurons revealed subunit-dependent localization that was not predicted by anal...
|
PMID: 21917805
PDF is available here.
PDF is available here.
| < More recent | Older article > |
Abstract
We found for the first time that betaAP (25-35 fragment, 10-6 M) disturbs the processes of the resting membrane potential generation in muscle fibres, leading to depolarization by two mechanisms: 1) inhibition of Na+,K(+)-ATPase, which leads to loss of impact of this pump to the resting membrane pot...
|
PMID: 21961303
PDF is available here.
PDF is available here.
Abstract
The purpose of this study was to provide direct evidences that rotor ablation suppresses atrial fibrillation (AF) inducibility.
|
PMID: 21718913
PDF is available here.
PDF is available here.
Abstract
We investigated this apparent discrepancy by studying the effects of in vivo conditions on VN neuron activity in vitro using a simple, physiologically based, model of cellular dynamics. We found that membrane potential oscillations were evoked both in response to step and zap current injection for a...
|
PMID: 21814508
PDF is available here.
PDF is available here.
Abstract
We examined the effects of the OR ligand thymol on ion transport in human and rat colonic epithelia using an Ussing chamber. In the mucosal-submucosal preparations, the mucosal addition of thymol evoked anion secretion concentration dependently. In addition, dextran (4 kDa) permeability was enhanced...
|
PMID: 21372164
PDF is available here.
PDF is available here.
Abstract
Hydrogen sulfide (H(2)S) is produced endogenously by L-cysteine metabolism. H(2)S modulates several ion channels with an unclear mechanism of action. A possible mechanism is through reduction-oxidation reactions attributable to the redox potential of the sulfur moiety. The aims of this study were to...
|
PMID: 21393430
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)9 2011
Abstract
The twin-arginine translocase (Tat) system is used by many bacteria and plants to move folded proteins across the cytoplasmic or thylakoid membrane. In most bacteria, the TatA protein is believed to form a defined pore in the membrane through homo-oligomerization with other TatA protomers. The predic...
|
PMID: 21683683
PDF is available here.
PDF is available here.
Abstract
We are interested to determine whether similar GPCR pathways can be used for chemosensory transduction in Tetrahymena. To confirm our procedures, we tested the known chemoattractant proteose peptone (at 1.0 mg/ml), which caused hyperpolarization and increased forward swimming speed in Tetrahymena, c...
|
PMID: 21091443
PDF is available here.
PDF is available here.
Abstract
We describe general characteristics of the Hodgkin-Huxley neuron's response to a periodic train of short current pulses with Gaussian noise. The deterministic neuron is bistable for antiresonant frequencies. When the stimuli arrive at the resonant frequency the firing rate is a continuous function o...
|
PMID: 21728566
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)8 2011
Abstract
We found that hKir2.1 current was reversibly inhibited by the broad spectrum PTK inhibitor genistein and the highly selective EGFR (epidermal growth factor receptor) kinase inhibitor AG556 in a concentration-dependent manner. The inhibition of hKir2.1 channels by genistein or AG556 was countered by...
|
PMID: 21570948
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)8 2011
Abstract
Molecular mechanisms of the influence of flavonoids on the voltage gating of a single alpha-hemolysin channel in planar lipid membranes are studied. It is shown that the addition of flavonoids hydroxylated in position 5 of the A-ring and in position 4' of the B-ring into bilayer bathing solution shif...
|
PMID: 21527242
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)9 2011
Abstract
We previously reported the existence of different TMEM16A isoforms generated by alternative splicing. In the present study, we have determined the functional properties of a minimal TMEM16A protein. This isoform, called TMEM16A(0), has a significantly shortened amino-terminus and lacks three alterna...
|
PMID: 21645494
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1807)7 2011
Abstract
Oxidative redox titrations of the mitochondrial cytochromes were performed in near-anoxic RAW 264.7 cells by inhibiting complex I. Cytochrome oxidation changes were measured with multi-wavelength spectroscopy and the ambient redox potential was calculated from the oxidation state of endogenous cytoch...
|
PMID: 21420929
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1808)8 2011
Abstract
We incorporate the electrostatic-hydrogen bond switch mechanism into the Gouy-Chapman model for a membrane that is composed of PA and a hydrogen bond-donating zwitterionic lipid. To this end, our model integrates into the Gouy-Chapman approach a recently suggested electrostatic model for zwitterioni...
|
PMID: 21406179
PDF is available here.
PDF is available here.
Abstract
We sought to determine whether loss of JPH2 expression is noted among patients with HCM and whether expression silencing might perturb Ca(2+) handling in a prohypertrophic manner.
JPH2 expression was reduced in flash-frozen human cardiac tissue procured from patients with HCM compare...
|
PMID: 21216834
PDF is available here.
PDF is available here.
Abstract
We sought to determine whether loss of JPH2 expression is noted among patients with HCM and whether expression silencing might perturb Ca(2+) handling in a prohypertrophic manner.
JPH2 expression was reduced in flash-frozen human cardiac tissue procured from patients with HCM compare...
|
PMID: 21216834
PDF is available here.
PDF is available here.
Long-term self-renewable feeder-free human induced pluripotent stem cell-derived neural progenitors.
Abstract
We have established a homogenous, expandable, and self-renewable population of multipotent NPs from hiPSCs, using an adherent system and defined medium supplemented with a combination of factors. The established hiPSC-NPs highly expressed Nestin and Sox1. These NPs were continuously propagated for ~...
|
PMID: 20632795
PDF is available here.
PDF is available here.
Abstract
We show that for a single postsynaptic neuron maximum energy efficiency, measured in bits of mutual information per molecule of adenosine triphosphate (ATP) consumed, requires maximum energy consumption. For groups of parallel postsynaptic neurons we determine values of the synaptic conductance at w...
|
PMID: 21517530
PDF is available here.
PDF is available here.
Abstract
We allowed SHR and control rats to drink 1% NaCl for two weeks. Again, proximal tubule peritubular membrane potential was not different in SHR on 1% NaCl (-67.0 ± 1.0 mV) as compared with control rats on 1% NaCl (-64.7 ± 1.3 mV). From these results we concluded that peritubular membrane potential...
|
PMID: 21388926
PDF is available here.
PDF is available here.
Abstract
We allowed SHR and control rats to drink 1% NaCl for two weeks. Again, proximal tubule peritubular membrane potential was not different in SHR on 1% NaCl (-67.0 ± 1.0 mV) as compared with control rats on 1% NaCl (-64.7 ± 1.3 mV). From these results we concluded that peritubular membrane potential...
|
PMID: 21388926
PDF is available here.
PDF is available here.
Abstract
CLC anion transport proteins function as Cl (-) channels and Cl (-) /H (+) exchangers and are found in all major groups of life including archaebacteria. Early electrophysiological studies suggested that CLC anion channels have two pores that are opened and closed independently by a...
|
PMID: 21317557
PDF is available here.
PDF is available here.
Abstract
We studied Xenopus laevis neural crest, an embryonic stem cell population that gives rise to many cell types, including melanocytes, and contributes to the morphogenesis of the face, heart and other complex structures. To investigate how depolarization of transmembrane potential of cells in the neur...
|
PMID: 20959630
PDF is available here.
PDF is available here.
Abstract
We focus on Nedd4 and Nedd4-2 ubiquitin ligases and the mechanisms by which they regulate voltage-gated ion channels and describe a novel paradigm on the mechanisms that underpin aberrant ion channel function in neurological disorders....
|
PMID: 21057195
PDF is available here.
PDF is available here.
Abstract
We have accomplished thermodynamic analysis of mouse TREK-1 gating and functional testing of several deletion mutants. The predicted increase of the channel in-plane area (ΔA) of ~5 nm(2) in models was supported by the experimental ΔA of ~4 nm(2) derived from the slope of open probability versus m...
|
PMID: 21057213
PDF is available here.
PDF is available here.
Abstract
Glial cells such as retinal Müller glial cells are involved in potassium ion and water homeostasis of the neural tissue. In these cells, inwardly rectifying potassium (Kir) channels and aquaporin-4 water channels play an important role in the process of spatial potassium buffering and water drainage...
|
PMID: 21283711
PDF is available here.
PDF is available here.
Abstract
We report that the binding of a murine monoclonal IgA (IgAC5) to the O-Ag of S. flexneri serotype 5a suppresses activity of the type 3 secretion (T3S) system, which is necessary for S. flexneri to gain entry into intestinal epithelial cells. IgAC5's effects on the T3S were rapid (5 to 15 min) and...
|
PMID: 21610121
PDF is available here.
PDF is available here.
Abstract
We will consider the influence of ion channels, as dynamic gatekeepers of membrane permeability, on urogenital function. Through their ability to act as key regulators of both the resting membrane potential and its dynamic changes, they provide important pharmacological targets for influencing uroge...
|
PMID: 21290236
PDF is available here.
PDF is available here.
Abstract
These results suggest that the endogenous NO may inhibit the tachykininergic neuro-muscular transmissions by the decrease of SP release from the tachykininergic neurons, possibly through a guanylate cyclase-cGMP-dependent mechanism in the hamster ileum....
|
PMID: 21139351
PDF is available here.
PDF is available here.
Abstract
We survey methods of optimizing the optical set-up, enhancing the β of the dye and achieving biological compatibility. In conclusion, we examine novel applications of SHG imaging and highlight promising directions for the development of the field....
|
PMID: 20820473
PDF is available here.
PDF is available here.
Journal of Biological Chemistry (285)45 2010
Abstract
We examined temporal (young, 0.5 months old, and senescent, 17 months old) and spatial (apical and basal) roles of K(v)7-mediated currents. We report that differential contribution of K(v)7 currents in mice SGNs results in distinct and profound variations of the membrane properties of basal versus a...
|
PMID: 20739290
PDF is available here.
PDF is available here.
Abstract
We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activ...
|
PMID: 20801170
PDF is available here.
PDF is available here.
Current Eye Research (35)11 2010
Abstract
Our results indicate that more RCS-ON-RBCs survived in the central retinal area near cone clusters, potentially as a result of ectopic neuritis. Meanwhile the surviving RCS-ON-RBCs remained immature and had no normal electrophysiological characteristics....
|
PMID: 20958189
PDF is available here.
PDF is available here.
Abstract
We show here by time-resolved optical and FTIR spectroscopy combined with time-resolved electrometry that some mutant enzymes incapable of proton pumping nevertheless initiate catalysis by proton transfer to a proton-loading site. A conserved tyrosine in the so-called D-channel is identified as a po...
|
PMID: 20937896
PDF is available here.
PDF is available here.
Abstract
Normal human epidermis possesses a transepithelial potential (TEP) that varies in different parts of the body (10–60mV). The role of TEP in normal epidermis is not yet identified; but after skin injury, TEP disruption induces an endogenous direct current electric field (100–200mV...
|
PMID: 20486795
PDF is available here.
PDF is available here.
Histology and histopathology (25)10 2010
Abstract
We investigated the intermediate- conductance Ca2+-activated K+ channels (hKCa3.1) expression in both breast carcinoma (BC) specimens and human breast cancer epithelial primary cell cultures (hBCE) using immuno-histochemistry (60 samples), quantitative Real-Time RT-PCR (30 samples) and Western blot...
|
PMID: 20712009
PDF is available here.
PDF is available here.
Abstract
We examined the effects of nominally selective inhibitors of PI3K (wortmannin, PI103, GDC-0941), SGK1 (GSK650394A) and PKB (Akti-1/2) on Na(+) transport in hormone-deprived and insulin-stimulated cortical collecting duct (mpkCCD) cells, while PI3K, SGK1 and PKB activities were assayed by monitoring...
|
PMID: 20880397
PDF is available here.
PDF is available here.
Abstract
K(v)11.1 channels are involved in regulating cellular excitability in various tissues including brain, heart and smooth muscle. In these tissues, at least two isoforms, K(v)11.1a and K(v)11.1b, with different kinetics, are expressed. K(v)11.1 activators are potential therapeutic agents, but their ef...
|
PMID: 20880400
PDF is available here.
PDF is available here.
Food and Chemical Toxicology (48)10 2010
Abstract
We assessed the capability of hesperidin to prevent tert-butyl hydroperoxide (t-BuOOH)-induced cell damage by augmenting cellular antioxidant defense. Hesperidin significantly protected hepatocytes against t-BuOOH-induced cell cytotoxicity, such as mitochondrial membrane potential (MMP) deplete and...
|
PMID: 20678535
PDF is available here.
PDF is available here.
Anesthesiology (113)4 2010
Abstract
We tested whether anesthetic-induced preconditioning (APC) with isoflurane elicits competent protective mechanisms in hESC-derived cardiomyocytes against oxidative stress to be used as a model of human cardiomyocytes for studying preconditioning.
H1 hESC cell line was differentiated into cardiomyocy...
|
PMID: 20823757
PDF is available here.
PDF is available here.
Abstract
We determined that LPS directly and reversibly affects cardiac myocyte function by altering regulation of intracellular Ca2+ concentration ([Ca2+]i) in immortalized cardiomyocytes, HL-1 cells. [Ca2+]i oscillated (<0.4 Hz), displaying slow and transient components. LPS (1 microg/ml), derived either f...
|
PMID: 20573997
PDF is available here.
PDF is available here.
Abstract
We hypothesized that PKC causes vasoconstriction by stimulating translocation of TRPM4 to the plasma membrane. Live-cell confocal imaging and fluorescence recovery after photobleaching (FRAP) analysis was performed using a green fluorescent protein (GFP)-tagged TRPM4 (TRPM4-GFP) construct expressed...
|
PMID: 20610768
PDF is available here.
PDF is available here.
Abstract
The data suggest that WJ9708011 induces transcriptional and translational suppression of cell-cycle regulators that might be through Akt- and AMPK-independent loss of DeltaPsi(m) and inhibition of mTOR signaling pathway, leading to G1 arrest of the cell cycle and subsequent apoptotic cell death.
(c)...
|
PMID: 20623631
PDF is available here.
PDF is available here.
Brain research (1349) 2010
Abstract
We have examined the effect of 5HT on electrical synapses possessing variable coupling strengths. While 5HT decreased electrical coupling at synapses with weak electrical connectivity, synapses with strong electrical coupling were less affected by 5HT treatment, as follows from the equations used fo...
|
PMID: 20599836
PDF is available here.
PDF is available here.
Abstract
Abstract
We propose that the loss of conductance is due to the induction, by H+ or Ni2+, of a resting inactivation process. To assess whether the two inactivation processes are mechanistically related, we examined the time courses for the onset of and recovery from H+- or Ni2+-enhanced slow inactivation and...
|
PMID: 20581043
PDF is available here.
PDF is available here.
Journal of the Neurological Sciences (295)1-2 2010
Abstract
We have investigated the influence of 15-hydroxyeicosatetrienoic acid (15-HETE) produced by 15-lipoxygenase (15-LOX) on vasoconstriction during hypoxia. As showed in our results, 15-LOX level increases in ICA endothelia and smooth muscles. 15-HETE enhances the tension of ICA ring in a dose-dependent...
|
PMID: 20483431
PDF is available here.
PDF is available here.
Abstract
Experimental evidence collected more than 20 years ago in different laboratories suggests that the interactions between live biological cells and micro- and nanoparticles depend on their metabolic state. These experiments were conducted by reputable groups, led by prominent leaders such as H. Pohl o...
|
PMID: 20637326
PDF is available here.
PDF is available here.
Physical Review E (82)2 Pt 1 2010
Abstract
We show using a FitzHugh-Nagumo excitable system that the second response threshold can be reached transiently by brief pulses in the negative noise component, leading to a coherence resonance phenomenon of its own. The resonance can occur both as a function of input amplitude and frequency. The phe...
|
PMID: 20866773
PDF is available here.
PDF is available here.
Abstract
Bacterial Trk and Ktr, fungal Trk and plant HKT form a family of membrane transporters permeable to K(+) and/or Na(+) and characterized by a common structure probably derived from an ancestral K(+) channel subunit. This transporter family, specific of non-animal cells, displays a lar...
|
PMID: 20333436
PDF is available here.
PDF is available here.
Abstract
The internal hydrostatic pressure (turgor) of the filamentous fungus Neurospora crassa is regulated at about 400-500 kiloPascals, primarily by an osmotic MAP kinase cascade which activates ion uptake from the extracellular medium and glycerol synthesis. In the absence of hyperosmotic stress, the phe...
|
PMID: 20546911
PDF is available here.
PDF is available here.
Neurotoxicity Research (18)2 2010
Abstract
We hypothesized that, in the treatment of ChEI poisoning, the high death rate may not be attributed to the insufficient muscarinic antagonism but to the lack of nicotinic antagonism. To test this hypothesis, we designed and synthesized benthiactzine, a drug that blocks both muscarinic acetylcholine...
|
PMID: 19830505
PDF is available here.
PDF is available here.
Abstract
Ropivacaine induced tonic inhibition of peak Na(+) currents of both subtypes in a dose- and frequency-dependent manner. rNav1.5 appeared to be more sensitive to ropivacaine. In addition, for both Na(+) channel subtypes, the steady-state inactivation curves, but not the activation curves, were signif...
|
PMID: 20651810
PDF is available here.
PDF is available here.
Abstract
We found that astrocytes of the brainstem chemoreceptor areas are highly chemosensitive. They responded to physiological decreases in pH with vigorous elevations in intracellular Ca2+ and release of adenosine triphosphate (ATP). ATP propagated astrocytic Ca2+ excitation, activated chemoreceptor neur...
|
PMID: 20647426
PDF is available here.
PDF is available here.
Abstract
We compared time courses of early signals induced by wounding in Lima bean. Neither MD nor MW alone was able to induce plasma membrane (V (m)) depolarization, as observed after Spodoptera littoralis HW, but V (m) depolarization occurred in both treatments when used in combination with herbivore-deri...
|
PMID: 20563731
PDF is available here.
PDF is available here.
Journal of Neurotrauma (27)8 2010
Abstract
We used a controlled cortical impact (CCI) model in rats to examine post-traumatic changes in neocortical excitability. Neocortical slices were prepared from rats at 7-9 days (week 1) and 14-16 days (week 2) after CCI injury. By week 2, we observed a substantial gray matter lesion with a cavity that...
|
PMID: 20504156
PDF is available here.
PDF is available here.
Abstract
We show that high-voltage activated Ca(2+) channels are regulated by membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) with different sensitivities. Plasma membrane PIP(2) depletion by rapamycin-induced translocation of an inositol lipid 5-phosphatase or by a voltage-sensitive 5-phosphatase (V...
|
PMID: 20670831
PDF is available here.
PDF is available here.
Abstract
We show that spontaneous calcium spike activity in the hindbrain of developing Xenopus laevis larvae modulates the specification of serotonergic neurons via regulation of expression of the Lmx1b transcription factor. Activity acts downstream of Nkx2.2 but upstream of Lmx1b, leading to regulation of...
|
PMID: 20670838
PDF is available here.
PDF is available here.
Abstract
COR-D possesses significant anti-leukemic activity in U937 and HL-60 cell lines by acting on the mitochondrial apoptotic pathways. Since the necrotic body formation is low after COR-D treatment, the occurrence of inflammation in in vivo systems could be reduced, which represents a positive indicatio...
|
PMID: 20033811
PDF is available here.
PDF is available here.
Abstract
We found that NO-generating substances (SNP and SNAP) activated the buccal rhythm while slowing the endogenous rhythm of B2 bursters. The inhibitor of NO-synthase, L-NNA, the NO scavenger PTIO, or the inhibitor of soluble guanylyl cyclase, ODQ, each produced opposite, depolarising effects on the B2...
|
PMID: 20559642
PDF is available here.
PDF is available here.
Cancer Letters (294)1 2010
Abstract
We have found that KG-135 potentates cytotoxicity of etoposide by modulating apoptotic signaling. Co-treatment of etoposide and KG-135 markedly elevated the expression and phosphorylation at the serine 15 residue of p53 as well as the cellular levels of Bax and p21(Waf1/Cip1). The increased accumula...
|
PMID: 20226587
PDF is available here.
PDF is available here.