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Welcome to the Pubget Medical Subject Headings (MESH) browser. Click on a topic or subtopic below to explore related papers. In the gold box is the most recent paper about the current MESH term, and below are 20 related papers.
Fluorescence (0):
Recent article
Fluorescence
Abstract
Amyloid-β protein (Aβ) is the principal component of the neuritic plaques found in Alzheimer's disease. The predominant Aβ morphology in the plaques is fibrillar which has prompted substantial in vitro work to better understand the molecular organization of Aβ fibrils. In the current study, trypt...
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PMID: 21843498
PDF is available here.
PDF is available here.
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Abstract
Medicinal plants have been widely recognized as a renewable resource for the discovery of novel leads and drug. In this study, an approach for screening and identification compounds with cardioprotective activity from medicinal plant extracts by cellular-fluorescence imaging technique was developed....
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PMID: 21819864
PDF is available here.
PDF is available here.
Abstract
We measured the fluorescence responses from single fusing vesicles expressing pHluorin (pHl), a pH-sensitive variant of GFP, fused to the luminal domain of the vesicular SNARE syb2 (spH) in cultured hippocampal neurons lacking endogenous syb2. Fluorescence responses were quantal, with the unitary si...
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PMID: 21844343
PDF is available here.
PDF is available here.
Abstract
Photophysical properties of a natural plant alkaloid, ellipticine (5,11-dimethyl-6H-pyrido[4,3-b]carbazole), which comprises both proton donating and accepting sites, have been studied in different solvents using steady state and time-resolved fluorescence techniques primarily to understand the origi...
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PMID: 21812494
PDF is available here.
PDF is available here.
Abstract
We were able to observe in 3D the presence of these cells within damaged cardiac tissue, with important structural details that are difficult to visualize by conventional bidimensional imaging techniques. This new 3D-imaging approach appears to be an important way to investigate the cellular events...
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PMID: 21360687
PDF is available here.
PDF is available here.
Ukrainskij biokhimicheskij zhurnal/Ukrayins'kij biokhimichnij zhurnal/Ukrainian biochemical journal (82)6 2011
Abstract
We suppose that diverse sensitivity to DT of monkey and mouse cells can be explained not only by differences in their receptor affinity for DT but also by the processes that occur after internalization of the toxin into the cells....
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PMID: 21805864
PDF is available here.
PDF is available here.
Medical Physics (38)7 2011
Abstract
We demonstrate that the Cerenkov emission can excite a fluorophore, protoporphyrin IX (PpIX), embedded in biological phantoms.
The results here indicate that molecular fluorescence monitoring during external beam radiotherapy is possible....
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PMID: 21859013
PDF is available here.
PDF is available here.
Abstract
We report the design and synthesis of a novel fluorescence probe for hydrogen peroxide, utilizing a photoinduced electron transfer strategy based on benzil chemistry to control the fluorescence. The practical value of this highly sensitive and selective fluorescence probe, NBzF, was confirmed by its...
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PMID: 21692459
PDF is available here.
PDF is available here.
Abstract
External electric field effects on absorption, fluorescence, and fluorescence decay of p-HBDI that is a model compound of the chromophore of GFP have been examined in a poly(methyl methacrylate) film. The electroabsorption spectrum is similar in shape to the first derivative of the absorption spectru...
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PMID: 21639105
PDF is available here.
PDF is available here.
Abstract
We suggest that the fastest decay component arises from a direct proton transfer process from the hydroxyl group of D-luciferin to the mild base. The proton donor and acceptor molecules form an ion pair prior to photoexcitation. Upon photoexcitation solvent rearrangement occurs on a 1 ps time-scale....
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PMID: 21630647
PDF is available here.
PDF is available here.
Abstract
We studied whether objective discrimination criteria can be set for this system when observing oral mucosal lesions. We examined 74 cases with biopsy-confirmed oral mucosal lesions; 37 squamous cell carcinoma lesions, 14 moderate to severe epithelial dysplasia lesions, 13 mild epithelial dysplasia l...
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PMID: 21827020
PDF is available here.
PDF is available here.
Journal of Trauma (70)6 2011
Abstract
We investigated the effectiveness of a mesenchymal stem cell (MSC)/hydroxyapatite/type I collagen hybrid graft for posterolateral spinal fusion in a rabbit model.
In vitro study, the hybrid graft was cultured in complete or osteogenic medium for 7 days and 14 days and examined by sca...
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PMID: 21817989
PDF is available here.
PDF is available here.
Abstract
Abstract
Mitochondria are most important organelles in the survival of eukaryotic aerobic cells because they are the primary producers of ATP, regulators of ion homeostasis or redox state, and producers of free radicals. The key role of mitochondria in the generation of primordial ATP for the...
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PMID: 21595013
PDF is available here.
PDF is available here.
Abstract
We immobilized primed DNA templates in the microreactors, then sequentially introduced one of the four identically labeled TPLFNs, sealed the microreactors and recorded a fluorescence image after template-directed primer extension. With cycle times of...
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PMID: 21666670
PDF is available here.
PDF is available here.
Abstract
We chose bicistronic lentiviral vectors coexpressing fluorescent reporters (eGFP, dTomato, Cerulean, or Venus) along with the canonical RFs to transduce a bulk of murine embryonic fibroblasts (MEFs). Using a flow cytometric approach, we were able to independently and proportionally quantify all fluo...
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PMID: 21548079
PDF is available here.
PDF is available here.
Abstract
We have developed a simple and controllable approach that allows construction of a two-level, three-dimensional (3D) networked structure to provide sufficient calcium source and achieve desired mechanical function and mass transport (permeability and diffusion) properties. Using a nontoxic cross-lin...
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PMID: 21491931
PDF is available here.
PDF is available here.
Physical Chemistry (62)1 2011
Abstract
We review the underlying principles and excitation and detection schemes, as well as exemplary biomedical applications of this emerging class of molecular imaging techniques....
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PMID: 21453061
PDF is available here.
PDF is available here.
Physical Chemistry (62)1 2011
Abstract
We review these FCS-based studies and the characteristic structural features of raft nanodomains. We also discuss the findings in regards to the current view of lipid rafts as a general membrane-organizing principle....
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PMID: 21219145
PDF is available here.
PDF is available here.
Physical Chemistry (62)1 2011
Abstract
We discuss here the detection of molecular transport by exploiting spatiotemporal correlations measured among points at large distances (>1 μm). We illustrate the evolution of the conceptual framework that started with single-point fluorescence fluctuation analysis based on the transit of fluoresce...
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PMID: 21219151
PDF is available here.
PDF is available here.
Abstract
We had to reduce the energy-transfer and charge-separation rates in complexes with decreasing size to obtain optimal fits. This strongly suggests that the antenna system is important for plant PSII integrity and functionality, which is supported by biochemical results. Furthermore, we used the coars...
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PMID: 21539776
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1807)9 2011
Abstract
We assume that conformational changes are only induced when Q(A) is reduced and relax rapidly on re-oxidation. This would explain the relationship between the fluorescence rise and the ETC-reduction.
Copyright © 2011. Published by Elsevier B.V....
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PMID: 21669182
PDF is available here.
PDF is available here.
Abstract
Intracellular viscosity strongly influences transportation of mass and signal, interactions between the biomacromolecules, and diffusion of reactive metabolites in live cells. Fluorescent molecular rotors are recently developed reagents used to determine the viscosity in solutions or...
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PMID: 21476543
PDF is available here.
PDF is available here.
Abstract
We use replica-exchange molecular dynamics simulations with an optimized all-atom protein force field (Amber ff03w) and an accurate water model (TIP4P/2005) to study the kinetics of helix formation in this peptide. We calculate temperature-dependent microscopic rate coefficients from the simulations...
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PMID: 21480610
PDF is available here.
PDF is available here.
Abstract
We present a cost-effective method for fluorescently end-labelling AFLPs that should make this tool more readily accessible for laboratories with limited budgets. Both standard fluorescent AFLPs and the end-labelled alternatives presented here are repeatable and produce similar numbers of fragments...
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PMID: 21481207
PDF is available here.
PDF is available here.
Abstract
A facile approach to prepare CdSe/ZnS quantum dot-encapsulated chitosan hybrid nanospheres (CS-QD) is developed by utilizing ethanol-aided counterion complexation in aqueous solution. The obtained CS-QD hybrid nanospheres have not only the loading space provided by the chitosan spher...
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PMID: 21388158
PDF is available here.
PDF is available here.
Abstract
We have evaluated three assay methods, making use of 59 marketed compounds and 28 novel GSK compounds. Recombinant bactosomes expressing the CYP3A4 isozyme were used with two fluorescence-based methods: a "Re-addition" assay and a "30 min" assay. The third method evaluated used pooled human liver mi...
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PMID: 21457139
PDF is available here.
PDF is available here.
Abstract
We performed a phenotype-driven ENU mutagenesis screen in mice with the aim of identifying novel alleles in an unbiased manner, that are critically required for early craniofacial development. Here we describe 10 new mutant lines, which exhibit phenotypes affecting frontonasal and pharyngeal arch pa...
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PMID: 21305688
PDF is available here.
PDF is available here.
Abstract
Detection of osteogenic differentiation is crucial for bone tissue engineering. Despite established standard end point assays, there is increasing demand for methods allowing noninvasive kinetic differentiation monitoring. Reporter gene assays employing tissue-specific promoters and...
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PMID: 21043997
PDF is available here.
PDF is available here.
Abstract
A far-red to near-infrared (NIR) fluorescence probe, MMSiR, based on Si-rhodamine, was designed and synthesized for sensitive and selective detection of HOCl in real time. MMSiR and its oxidized product SMSiR have excellent properties, including pH-independence of fluorescence, high resistance to aut...
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PMID: 21443186
PDF is available here.
PDF is available here.
Abstract
We coupled a specific anti-CysLT1R IgG antibody to near-infrared (NIR) hemicyanine fluorophore DY-734. The fluorophore was also coupled to unspecific rabbit-IgG antibody or corresponding Fab fragments. Expression of CysLT1R in HL-60 human promyelocytic leukemia cells in vitro could be proven by reve...
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PMID: 21439253
PDF is available here.
PDF is available here.
Biochimica et Biophysica Acta (1807)7 2011
Abstract
We show that Guillardia theta is able to perform non-photochemical quenching, although none of the usual xanthophyll cycle pigments (e.g., zeaxanthin, diadinoxanthin, diatoxanthin) are present at detectable levels. Instead, acclimation to high light intensity seems to involve an increase of alloxant...
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PMID: 21459077
PDF is available here.
PDF is available here.
Abstract
We have previously shown that the PNN can bind large amounts of iron due to its polyanionic charge. Because free iron can generate reactive oxygen species thus being potentially toxic, the PNN may have a protective function by "scavenging" this free iron. Because of this ability, we have hypothesize...
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PMID: 21425238
PDF is available here.
PDF is available here.
Abstract
We characterized metabolic C. elegans mutants by using novel and feasible BODIPY 493/503-based fat staining and flow cytometry approaches. Fixative as well as vital BODIPY staining procedures visualize major fat stores, preserve native lipid droplet morphology, and allow quantification of fat conten...
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PMID: 21421847
PDF is available here.
PDF is available here.
Abstract
Under specific light illumination, particularly ultraviolet (UV) and near-UV light stimulation, the skin produces both specular light reflectance and, possibly, specific fluorescent emission. These properties offer diagnostic clues and disclose some peculiar functions of the skin. A series of superfi...
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PMID: 21411414
PDF is available here.
PDF is available here.
Biopolymers (95)7 2011
Abstract
We employed such system to investigate melting transitions of ordered nucleic acid structures into disordered random coils. Fluorescent dye and quencher were attached to oligonucleotides in such a way that changes of fluorescence intensity with temperature indicated progression of denaturation. When...
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PMID: 21384337
PDF is available here.
PDF is available here.
Abstract
The merge of embryonic stem cell technology and developmental neurobiology provides valuable tools to decipher the mechanisms underlying human brain development and diseases, especially for neuronal populations that are not readily available in primary cultures. It is obviously the c...
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PMID: 21418521
PDF is available here.
PDF is available here.
Operative Dentistry (36)1 2011
Abstract
To (1) identify the methods that dentists in The Dental Practice-Based Research Network (DPBRN) use to diagnose dental caries; (2) quantify their frequency of use and (3) test the hypothesis that certain dentist and dental practice characteristics are significantly associated with their use.
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PMID: 21488724
PDF is available here.
PDF is available here.
Abstract
To study the value of fluorescence quantitative polymerase chain reaction (FQ-PCR) for detecting Mycoplasma pneumoniae (MP)-DNA in bronchoalveolar lavage fluid (BALF) in the early diagnosis of Mycoplasma pneumoniae pneumonia (MPP).
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PMID: 21426633
PDF is available here.
PDF is available here.
Monaldi archives for chest disease = Archivio Monaldi per le malattie del torace / Fondazione clinica del lavoro, IRCCS [and] Istituto di clinica tisiologica e malattie apparato respiratorio, Universita di Napoli, Secondo ateneo (75)1 2011
Abstract
Community dental health (28)1 2011
Abstract
These results suggest that although DIAGNOdent is not a substitute method for the radiographic examination in surveys, it may be an alternative as an auxiliary to visual inspection....
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PMID: 21485229
PDF is available here.
PDF is available here.
Abstract
We create unique particles that have all the necessary physical characteristics to serve as in vivo reporters, but with minimized diffusion from the point of injection. These particles, called microworms, have a cylindrical shape coated with a biocompatible porous membrane that possesses a large sur...
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PMID: 21282619
PDF is available here.
PDF is available here.
Abstract
We have focused on LAT ubiquitylation and have mapped the sites on which LAT is ubiquitylated. To elucidate the biological role of this process, we substituted LAT lysines with arginines. This resulted in a dramatic decrease in overall LAT ubiquitylation. Ubiquitylation-resistant mutants of LAT were...
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PMID: 21282648
PDF is available here.
PDF is available here.
Abstract
Abstract
We have investigated the use of a fluorescent image analyser and the application of fluorescence intensity measurements to describe and evaluate a tissue-engineered cartilage-like tissue construct. We evaluated the matrix production of a tissue-engineered cartilage-like tissue (TCL) construct in vit...
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PMID: 20734346
PDF is available here.
PDF is available here.
Abstract
We establish an intestinal epithelia barrier model using Caco-2 cells in vitro. Transepithelial electrical resistance and unidirectional flux of lucifer yellow were measured to evaluate barrier permeability; immunofluorescent staining and flow cytometry were applied to observe morphological alterati...
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PMID: 21380808
PDF is available here.
PDF is available here.
Abstract
To determine the origin of fundus autofluorescence (AF) patterns in central serous chorioretinopathy (CSC).
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PMID: 21257153
PDF is available here.
PDF is available here.
Journal of Molecular Biology (405)3 2011
Abstract
We report a fluorescence-based assay for measuring the real-time kinetics of single-nucleotide incorporation during transcription elongation. The fluorescent adenine analogue 2-aminopurine was incorporated at various single positions in the template or the nontemplate strand of the promoter-free elo...
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PMID: 21035457
PDF is available here.
PDF is available here.
Abstract
We demonstrate that the enzyme β-lactamase, a naturally occurring enzyme expressed by M. tuberculosis, can be used for REF. The resulting imaging system is the first that allows non-invasive detection of natural M. tuberculosis strains directly in pulmonary infected living animals. Use of REF for M...
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PMID: 21178505
PDF is available here.
PDF is available here.
Abstract
We characterise how the two noise sources confinement and burst modulate the relaxation kinetics of a non-linear reaction network around a non-equilibrium steady state. We find that the lifetimes of species change with burst input and confinement. Confinement increases the lifetimes of all species t...
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PMID: 21297975
PDF is available here.
PDF is available here.
Abstract
We investigated if commercially available compact fluorescent lamps with different colour temperatures can impact on alertness and cognitive performance....
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PMID: 21298068
PDF is available here.
PDF is available here.
Abstract
We investigated several different light scattering and fluorescence measurements in several different cell lines, sorting cell fractions from the high and low end of distributions, and measuring volume electronically to determine which sorting strategy yielded the best separated volume distributions...
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PMID: 21283800
PDF is available here.
PDF is available here.
Abstract
We outline the steps necessary to apply the FCB method to cell lines, as well as primary peripheral blood samples. Important technical considerations, such as choice of barcoding dyes, concentrations, labeling buffers, compensation, and software analysis, are discussed....
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PMID: 21207359
PDF is available here.
PDF is available here.
Abstract
We have co-infected Phlebotomus perniciosus and Lutzomyia longipalpis with transgenic promastigotes of Leishmania donovani strains carrying hygromycin or neomycin resistance genes and red or green fluorescent markers. Fed females were dissected at different times post bloodmeal (PBM) and examined by...
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PMID: 21637755
PDF is available here.
PDF is available here.
Abstract
Nanomaterials and nanostructures exhibit unique size-tunable and shape-dependent physicochemical properties that are different from those of bulk materials. Advances of nanomaterials and nanostructures open a new door to develop various novel biosensors. The present work has reviewed the recent progr...
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PMID: 21622273
PDF is available here.
PDF is available here.
Abstract
Single wall Carbon Nanotubes (SWCNTs) are hydrophobic and do not disperse in aqueous solvents. Acid functionalization of SWCNTs results in attachment of carboxy and sulfonate groups to carbon atoms and the resulting acid functionalized product (AF-SWCNTs) is negatively charged and disperses easily in...
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PMID: 21818289
PDF is available here.
PDF is available here.
Abstract
We aim at setting up and validating a blood test to evaluate CFTR expression and function in leukocytes....
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PMID: 21811577
PDF is available here.
PDF is available here.
Abstract
Glioma cells are exposed to elevated interstitial fluid flow during the onset of angiogenesis, at the tumor periphery while invading normal parenchyma, within white matter tracts, and during vascular normalization therapy. Glioma cell lines that have been exposed to fluid flow forces...
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PMID: 21637818
PDF is available here.
PDF is available here.
Abstract
Results of rapid laser-assisted identification of microorganisms for diagnostics of microbial processes based on auto-fluorescence effect in bacteria-containing materials are summarized. It is proposed to use the auto-fluorescence technique for express diagnostics of pyoinflammatory diseases, evaluat...
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PMID: 21395094
PDF is available here.
PDF is available here.
Voprosy Virusologii (56)1 2011
Abstract
A real-time fluorescent polymerase chain reaction was used to develop a RealBest HIV RNA kit that was clinically suitable for the detection of HIV-1 RNA and for the estimation of virus load in plasma and serum samples. Due to the selection of a highly conserved target region and to the experimental s...
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PMID: 21427951
PDF is available here.
PDF is available here.