Abstract
We developed a regression approach to correct for variability in fluorescence intensity due to differences in cell size and granularity without discarding any of the cells, which gating ipso facto does. This approach enables quantitative studies of cellular heterogeneity and transcriptional noise in...
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PMID: 21952134
PDF is available here.
Abstract
We developed a binary mixing system with two Pluronics, L121/P123, as a nano-sized drug delivery carrier. The lamellar-forming Pluronic L121 (0.1 wt%) was incorporated with Pluronic P123 to produce nano-sized dispersions (in case of 0.1 and 0.5 wt% P123) with high stability due to Pluronic P123 and...
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PMID: 20850281
PDF is available here.
Abstract
We demonstrate that the gene mafba (valentino) is expressed in CoPA interneurons....
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PMID: 21720556
PDF is available here.
Abstract
We successfully propagated floating spheres with stem cell properties in the SGC-7901 gastric cell line....
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PMID: 21789022
PDF is available here.
Author(s) unavailable
Abstract
The molecular mobility of the fluorescent probe, N-(carboxymethyl)imide of 4-(dimethylamino)naphthalic acid (K-35) in three types of binding sites on a human serum albumin (HSA) molecule has been studied. The time-resolved decay of K-35 polarized fluorescence in HSA has been studied and it has been s...
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PMID: 21786694
PDF is available here.
Abstract
We examined the conformational transitions in a multimeric and high molecular weight class II α-mannosidase from Canavalia ensiformis (Jack Bean) (Jbα-man) utilizing intrinsic fluorescence, solute quenching, hydrophobic dye binding, size exclusion chromatography and circular dichroism (CD) spectro...
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PMID: 21087596
PDF is available here.
Abstract
We demonstrate hybrid multifluorophore DNA-photonic wires that both self-assemble around semiconductor quantum dots (QDs) and exploit their unique photophysical properties. In this architecture, the QDs function as both central nanoscaffolds and ultraviolet energy harvesting donors that drive Förs...
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PMID: 21141858
PDF is available here.
Abstract
I and PS II fluorescence were calculated using a model that considers PS I fluorescence to be constant but variable PS II fluorescence. The photosystem spectra suggest that LHC II absorption results in high values of F(V)/F(M) between 470 and 490 nm in the two wild-type leaves but the absence of LHC...
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PMID: 19544007
PDF is available here.
Abstract
The spectroscopic behavior of lumichrome (7,8-dimethyl-alloxazine, LC) in aqueous solutions in a pH range from -1.08 to 14.6 is studied. Absorption spectra, fluorescence quantum distributions, quantum yields, and lifetimes are determined. The ionization stage of ground-state LC changes with rising pH...
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PMID: 21091486
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Abstract
We performed serial MPM and mechanical tests on acellular and cellularized (normal human lung fibroblasts) collagen hydrogels, before and after glutaraldehyde crosslinking. Following gel contraction over 16 days, cellularized collagen gel content approached that of native connective tissues (∼200...
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PMID: 20620246
PDF is available here.
Abstract
An unexpected discovery of new trans-4-acetyl-1,9-dimethyl-4,4a-dihydro-3H-fluoren-3-ones from one pot reactions of benzaldehydes and acetylacetone is described. The synthetic mechanism and stereochemistry were discussed. These new derivatives exhibit good fluorescent properties in solutions.
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PMID: 20820667
PDF is available here.
Abstract
3-Aminophenyl-modified analogues of the bicyclic nucleoside N-methyl-3H-pyrrolo[2,3-d]pyrimidin-2(7H)-one were synthesised and incorporated directly into triplex-forming oligonucleotides in order to utilise their extended hydrogen bonding motif for recognition of the CG base pair. All analogues demo...
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PMID: 20835452
PDF is available here.
Abstract
A promising one-step homogeneous fluoroimmunoassay based on nanoscale-graphene sheets as powerful fluorescence acceptors and CdTe quantum dots as vigorous donors was designed to detect trace biomarker protein with distance-independent quenching efficiency, which significantly broke the distance limi...
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PMID: 20856985
PDF is available here.
Abstract
We present an efficient, yet inexpensive, approach for isolating viable single cells or colonies from a mixed population. This cell microarray platform possesses innovations in both the array manufacture and the manner of target cell release. Arrays of microwells with bases composed of detachable co...
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PMID: 20838672
PDF is available here.
Abstract
Fluorescence-enhanced optical imaging using near-infrared (NIR) light developed for in vivo molecular targeting and reporting of various diseases provides promising opportunities for diagnostic imaging. However, the measurement sensitivity of NIR fluorescence (NIRF) optical imaging systems is limited...
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PMID: 21158309
PDF is available here.
Abstract
We have developed a molecular logic gate for the recognition of PPi with a fluorescent hypocrellin A-zinc(II) complex (HA-Zn(2+)), and the outputs of which correspond to the fluorescence emission ratio at 604 nm and wavelength shift relative to 629 nm. The emission at 604 nm is the intrinsic fluores...
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PMID: 20877861
PDF is available here.
Abstract
This paper describes the use of microarray-based biosensor system for the determination of phenol. Microarrays based on poly(ethylene glycol)(PEG) hydrogel were prepared by photopatterning of a solution containing PEG diacrylate (PEG-DA), photoinitiator, tyrosinase, and CdSe/ZnS quantum dots (QDs)....
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PMID: 20852777
PDF is available here.
Abstract
A simple and sensitive approach for the recognition of single base mismatches in duplex DNA was developed. The single base mismatched double-strand (ds) DNA and the completely complementary dsDNA can quench the fluorescence of methylene blue to the different values, so the point mutation dsDNA can b...
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PMID: 20830326
PDF is available here.
Abstract
Herein, a sensitive and selective sensor for mercury(II) and glutathione based on the aggregation-induced emission (AIE) of a tetraphenylethene derivative stimulated by Hg(2+)-DNA complexes is reported. Aggregation complexes of AIE probes, quaternized tetraphenylethene salt and anti-Hg(2+) aptamer s...
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PMID: 20877906
PDF is available here.
Abstract
We describe fluorescence-based 2-D gel electrophoresis methods for visualization of low abundant, cancer relevant tyrosine phosphorylated (pTyr) proteins. The methods investigated were fluorescent Western blotting and two-dimensional difference gel electrophoresis (2-D DIGE) for detection of non-enr...
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PMID: 20888324
PDF is available here.